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The map label for this gene is oprF [H]

Identifier: 157960193

GI number: 157960193

Start: 420184

End: 420789

Strand: Reverse

Name: oprF [H]

Synonym: Spea_0364

Alternate gene names: 157960193

Gene position: 420789-420184 (Counterclockwise)

Preceding gene: 157960194

Following gene: 157960192

Centisome position: 8.13

GC content: 44.88

Gene sequence:

>606_bases
ATGAAAATCATACTAGTTCTATTAAGTCTCGCGATTTTAGGTGGCTGCTCAATGCGTGATATTGCCACCATGGATGAGCC
AACGGTACAGGTTAACGATCTAAGCGATATCGATCATGATGGTGTGATTGTAGCAAGAGAGCGCTGTAACGAAACTGCGC
TTGGAGCAACTATCGATAACTATGGTTGCGGTAAAATCAAGCCAATTAATGAGCGTCAGGAGCTCAAGATCCTGTTTGCT
AATGACTCTTACTATATCGATCCTCAGTATTATCCGCAGGTAGAAGTGATCGCAAGCTTTATGCAGAAGTTCCCTAATAC
CCAAGCGGTTATTGAGGGCCACTGTAGTAAAACGGGATCACACCAACATAACCAAGTCCTGTCTCAAAATCGTGCCAATG
CAGTCAGCTCTCTACTTGCTGAACGTTTTGGTATTGATTCAGGTAGACTCAGTGCAGTAGGTTATAGCTTCGATCGTCCT
ATCGATCCGACTCATACCGCATCAGCACATAAGATAAACCGTCGAGTCATCGCTGAACTCACTGGCGACGACACCACTGC
GGATATGAAGTGGCACATCTACACGGTAGACGAAGAAGTTAAATAG

Upstream 100 bases:

>100_bases
ACCGCGTATTAAATGCCACCGGGCAACTGCTCGATTCACTGCGCGTGACTAGACCCGATGTCTGGAATGGAGAGAGAGAT
TACGAAGGAGGAGTCCCACA

Downstream 100 bases:

>100_bases
AAATCGTTCTGGAGCACCATGGTTGGCAGGCTTTATCAATTAAAAAAATTACGAAAAGTCTGCCAATTAAAATTGACGGT
ATTGGCTCTGTCTGTCTCTA

Product: OmpA/MotB domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MKIILVLLSLAILGGCSMRDIATMDEPTVQVNDLSDIDHDGVIVARERCNETALGATIDNYGCGKIKPINERQELKILFA
NDSYYIDPQYYPQVEVIASFMQKFPNTQAVIEGHCSKTGSHQHNQVLSQNRANAVSSLLAERFGIDSGRLSAVGYSFDRP
IDPTHTASAHKINRRVIAELTGDDTTADMKWHIYTVDEEVK

Sequences:

>Translated_201_residues
MKIILVLLSLAILGGCSMRDIATMDEPTVQVNDLSDIDHDGVIVARERCNETALGATIDNYGCGKIKPINERQELKILFA
NDSYYIDPQYYPQVEVIASFMQKFPNTQAVIEGHCSKTGSHQHNQVLSQNRANAVSSLLAERFGIDSGRLSAVGYSFDRP
IDPTHTASAHKINRRVIAELTGDDTTADMKWHIYTVDEEVK
>Mature_201_residues
MKIILVLLSLAILGGCSMRDIATMDEPTVQVNDLSDIDHDGVIVARERCNETALGATIDNYGCGKIKPINERQELKILFA
NDSYYIDPQYYPQVEVIASFMQKFPNTQAVIEGHCSKTGSHQHNQVLSQNRANAVSSLLAERFGIDSGRLSAVGYSFDRP
IDPTHTASAHKINRRVIAELTGDDTTADMKWHIYTVDEEVK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 22325; Mature: 22325

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIILVLLSLAILGGCSMRDIATMDEPTVQVNDLSDIDHDGVIVARERCNETALGATIDN
CHHHHHHHHHHHHCCCCCHHCCCCCCCCEEECCCCCCCCCCEEEECCCCCCCCCCCEECC
YGCGKIKPINERQELKILFANDSYYIDPQYYPQVEVIASFMQKFPNTQAVIEGHCSKTGS
CCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCC
HQHNQVLSQNRANAVSSLLAERFGIDSGRLSAVGYSFDRPIDPTHTASAHKINRRVIAEL
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHEEEEEE
TGDDTTADMKWHIYTVDEEVK
CCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MKIILVLLSLAILGGCSMRDIATMDEPTVQVNDLSDIDHDGVIVARERCNETALGATIDN
CHHHHHHHHHHHHCCCCCHHCCCCCCCCEEECCCCCCCCCCEEEECCCCCCCCCCCEECC
YGCGKIKPINERQELKILFANDSYYIDPQYYPQVEVIASFMQKFPNTQAVIEGHCSKTGS
CCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCC
HQHNQVLSQNRANAVSSLLAERFGIDSGRLSAVGYSFDRPIDPTHTASAHKINRRVIAEL
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHEEEEEE
TGDDTTADMKWHIYTVDEEVK
CCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]