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The map label for this gene is ilvG [H]

Identifier: 157960174

GI number: 157960174

Start: 391294

End: 393003

Strand: Direct

Name: ilvG [H]

Synonym: Spea_0345

Alternate gene names: 157960174

Gene position: 391294-393003 (Clockwise)

Preceding gene: 157960173

Following gene: 157960175

Centisome position: 7.56

GC content: 51.11

Gene sequence:

>1710_bases
ATGGAACGAGGGCAAATGGATCAGAGTCAAATGGAAGCTGGGCAGATGATACGAGGCGCTGATGCCGTCATAAAAGCGAT
TGCCGCACACGGTGTAACCACAGTATTTGGTTATCCTGGGGGGGCGATTATGCCTATCTATGACGCACTCTATGGTAGCC
CGGTAGAACATCTTCTCAGTCGTCATGAGCAAGGTGCTGCATTTGCAGCTGTGGGTTACGCCAGAGCCAGTGGCCAAACT
GGAGTGTGTTTTGCGACTTCCGGCCCAGGTGCTACTAACTTAGTGACCTCACTTGCCGATGCCCTGCTAGATTCTGTTCC
CGTTGTAGCCATCACAGGCCAAGTATCTACCGCCGTTATTGGTACCGACGCCTTCCAAGAGGTCGATGTTCTAGGCATGT
CGTTATCGTGTACTAAGCACAGCTTTATGGTGACCCATGTCGATGAGTTAGTTCCTACTCTCTATAAAGCATTCGAGATT
GCCGCATCTGGGCGTCCAGGTCCTGTTCTGGTCGATATCCCAAAAGATGTGCAAATCGCCATGCTGGAATATAAGACGCC
ACTGCAGGCGGTGACGCAAGAGCCAGAGCATACGCCAGAGCAAATAAATGATGCCCGCGAGCTTATCAGCCAAGCTAAGC
AGCCTATGCTCTATGTGGGCGGCGGTGTTGGCATGGCAAGTGCGGTGGATCAGCTACGAGACTTTATCAAGGTGACAGGC
ATTCCATCGGTTGCGACATTGAAAGGGCTAGGCTCAATCACTATCGATACGCCGGGCTACTTAGGCATGCTAGGTATGCA
TGGCTCAAAGGCGGCTAACTTAGCAGTGCAAGAGTGCGACTTGTTGATTGTTGTTGGCGCGCGATTTGATGATAGGGTGA
CAGGCCGTCTAGCTAGCTTCGCCGAAAATGCCAAGGTGATCCACTTAGATATCGATGCCGCAGAGCTAGGCAAACTACGT
CTACCTGAAGTGGCCATAGCTGGCGATCTACGCCAGATCCTTCCTGCGTTAACTCAAAACTTGGGCGAGCAATTTGCGCC
ATGGCAAGCCAAGGTTGCACAGATGAAGCAGGAATTTCAATGGCGTTATGACCGTCCCGGTGAGCTTATCTATGCGCCAG
CCATGCTGAATCGTCTTGCTCATAAGTTACCCCAAGACAGTGTTGTAGCTTGTGATGTCGGCCAGCACCAGATGTGGGTT
GCCCAGCATATGTTCTTTAATCGCCCGGAAGATCACCTATCGAGTGCAGGCCTTGGCACCATGGGCTTTGGCTTACCAGC
GGCCATCGGCGCCAAGGTTGCACGCCCAGATGCATGTGTTGTAGTTGTCTCTGGCGATGGCTCTTTTATGATGAATGTAC
AGGAGCTGACCACCATAAAGCGTAAGCGCTTACCGGTAAAAATCTTACTTGTCGATAACCAAAAGCTGGGCATGGTCAAG
CAATGGCAGCAGCTGTTTTTTGAAGAGCGTTATAGCGAGACAGACCTTTCCGATAACCCTGATTTCGTCAAGATGGCGTC
GGCGTTCGATATCCCAGGGCGCAATATCTATAAGGCCTGCGATGTAGAGGCGGGCTTAGATGAAATGCTTAATAGCCAAG
GACCATTTTTACTGCATGTGTCGATAGATGATGCCCATAACGTGTGGCCGCTAGTGCCGCCAGGGGCATCGAATCAAGAC
ATGATGGAAGAGATGGATAAGCAAACTTAA

Upstream 100 bases:

>100_bases
TCCGAAAGGAACGGGGGTTTTTTCGTATAAGGACCTAGATAAAGCATAACTCTTATCTAAGTCATTATGAAATAAGTGAA
TGGTAGCGGAGCGCGGATAT

Downstream 100 bases:

>100_bases
TCGTCTCGGCTTTGAGTTTGAAACACAGAATTAAAGCACTGAAGCATTAGCGATAACACCAGATGGAGAGCAAGAATATG
AACAATTACTCATTAGCGTT

Product: acetolactate synthase 2 catalytic subunit

Products: NA

Alternate protein names: AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit [H]

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MERGQMDQSQMEAGQMIRGADAVIKAIAAHGVTTVFGYPGGAIMPIYDALYGSPVEHLLSRHEQGAAFAAVGYARASGQT
GVCFATSGPGATNLVTSLADALLDSVPVVAITGQVSTAVIGTDAFQEVDVLGMSLSCTKHSFMVTHVDELVPTLYKAFEI
AASGRPGPVLVDIPKDVQIAMLEYKTPLQAVTQEPEHTPEQINDARELISQAKQPMLYVGGGVGMASAVDQLRDFIKVTG
IPSVATLKGLGSITIDTPGYLGMLGMHGSKAANLAVQECDLLIVVGARFDDRVTGRLASFAENAKVIHLDIDAAELGKLR
LPEVAIAGDLRQILPALTQNLGEQFAPWQAKVAQMKQEFQWRYDRPGELIYAPAMLNRLAHKLPQDSVVACDVGQHQMWV
AQHMFFNRPEDHLSSAGLGTMGFGLPAAIGAKVARPDACVVVVSGDGSFMMNVQELTTIKRKRLPVKILLVDNQKLGMVK
QWQQLFFEERYSETDLSDNPDFVKMASAFDIPGRNIYKACDVEAGLDEMLNSQGPFLLHVSIDDAHNVWPLVPPGASNQD
MMEEMDKQT

Sequences:

>Translated_569_residues
MERGQMDQSQMEAGQMIRGADAVIKAIAAHGVTTVFGYPGGAIMPIYDALYGSPVEHLLSRHEQGAAFAAVGYARASGQT
GVCFATSGPGATNLVTSLADALLDSVPVVAITGQVSTAVIGTDAFQEVDVLGMSLSCTKHSFMVTHVDELVPTLYKAFEI
AASGRPGPVLVDIPKDVQIAMLEYKTPLQAVTQEPEHTPEQINDARELISQAKQPMLYVGGGVGMASAVDQLRDFIKVTG
IPSVATLKGLGSITIDTPGYLGMLGMHGSKAANLAVQECDLLIVVGARFDDRVTGRLASFAENAKVIHLDIDAAELGKLR
LPEVAIAGDLRQILPALTQNLGEQFAPWQAKVAQMKQEFQWRYDRPGELIYAPAMLNRLAHKLPQDSVVACDVGQHQMWV
AQHMFFNRPEDHLSSAGLGTMGFGLPAAIGAKVARPDACVVVVSGDGSFMMNVQELTTIKRKRLPVKILLVDNQKLGMVK
QWQQLFFEERYSETDLSDNPDFVKMASAFDIPGRNIYKACDVEAGLDEMLNSQGPFLLHVSIDDAHNVWPLVPPGASNQD
MMEEMDKQT
>Mature_569_residues
MERGQMDQSQMEAGQMIRGADAVIKAIAAHGVTTVFGYPGGAIMPIYDALYGSPVEHLLSRHEQGAAFAAVGYARASGQT
GVCFATSGPGATNLVTSLADALLDSVPVVAITGQVSTAVIGTDAFQEVDVLGMSLSCTKHSFMVTHVDELVPTLYKAFEI
AASGRPGPVLVDIPKDVQIAMLEYKTPLQAVTQEPEHTPEQINDARELISQAKQPMLYVGGGVGMASAVDQLRDFIKVTG
IPSVATLKGLGSITIDTPGYLGMLGMHGSKAANLAVQECDLLIVVGARFDDRVTGRLASFAENAKVIHLDIDAAELGKLR
LPEVAIAGDLRQILPALTQNLGEQFAPWQAKVAQMKQEFQWRYDRPGELIYAPAMLNRLAHKLPQDSVVACDVGQHQMWV
AQHMFFNRPEDHLSSAGLGTMGFGLPAAIGAKVARPDACVVVVSGDGSFMMNVQELTTIKRKRLPVKILLVDNQKLGMVK
QWQQLFFEERYSETDLSDNPDFVKMASAFDIPGRNIYKACDVEAGLDEMLNSQGPFLLHVSIDDAHNVWPLVPPGASNQD
MMEEMDKQT

Specific function: Catalyzes the first step in the biosynthesis of branched-chain amino acids [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=519, Percent_Identity=27.9383429672447, Blast_Score=169, Evalue=7e-42,
Organism=Homo sapiens, GI93004078, Length=563, Percent_Identity=24.6891651865009, Blast_Score=148, Evalue=2e-35,
Organism=Escherichia coli, GI1790104, Length=550, Percent_Identity=46.5454545454545, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI87081685, Length=574, Percent_Identity=40.5923344947735, Blast_Score=428, Evalue=1e-121,
Organism=Escherichia coli, GI1786717, Length=561, Percent_Identity=30.1247771836007, Blast_Score=258, Evalue=8e-70,
Organism=Escherichia coli, GI1787096, Length=538, Percent_Identity=27.6951672862454, Blast_Score=178, Evalue=1e-45,
Organism=Escherichia coli, GI1788716, Length=551, Percent_Identity=25.2268602540835, Blast_Score=148, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=555, Percent_Identity=26.3063063063063, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=555, Percent_Identity=26.3063063063063, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=535, Percent_Identity=25.981308411215, Blast_Score=133, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6323755, Length=577, Percent_Identity=43.8474870017331, Blast_Score=454, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6320816, Length=475, Percent_Identity=23.7894736842105, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321524, Length=480, Percent_Identity=25, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323163, Length=477, Percent_Identity=23.8993710691824, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320123, Length=496, Percent_Identity=21.7741935483871, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323073, Length=479, Percent_Identity=24.2171189979123, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922626, Length=504, Percent_Identity=26.3888888888889, Blast_Score=164, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61400; Mature: 61400

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERGQMDQSQMEAGQMIRGADAVIKAIAAHGVTTVFGYPGGAIMPIYDALYGSPVEHLLS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCHHHHHHH
RHEQGAAFAAVGYARASGQTGVCFATSGPGATNLVTSLADALLDSVPVVAITGQVSTAVI
CCCCCCEEEEEHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCEEEEE
GTDAFQEVDVLGMSLSCTKHSFMVTHVDELVPTLYKAFEIAASGRPGPVLVDIPKDVQIA
CCCHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEE
MLEYKTPLQAVTQEPEHTPEQINDARELISQAKQPMLYVGGGVGMASAVDQLRDFIKVTG
EEECCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCC
IPSVATLKGLGSITIDTPGYLGMLGMHGSKAANLAVQECDLLIVVGARFDDRVTGRLASF
CCCHHHHHCCCEEEECCCCCEEEECCCCCHHHHEEHCCCCEEEEECCCCCCHHHHHHHHH
AENAKVIHLDIDAAELGKLRLPEVAIAGDLRQILPALTQNLGEQFAPWQAKVAQMKQEFQ
HCCCEEEEEECCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
WRYDRPGELIYAPAMLNRLAHKLPQDSVVACDVGQHQMWVAQHMFFNRPEDHLSSAGLGT
CCCCCCCCEEECHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHCCCCCC
MGFGLPAAIGAKVARPDACVVVVSGDGSFMMNVQELTTIKRKRLPVKILLVDNQKLGMVK
CCCCCHHHHCCCCCCCCEEEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCCHHH
QWQQLFFEERYSETDLSDNPDFVKMASAFDIPGRNIYKACDVEAGLDEMLNSQGPFLLHV
HHHHHHHHHHCCCCCCCCCCCCEEHHHHCCCCCCCHHHHCCCCCCHHHHHCCCCCEEEEE
SIDDAHNVWPLVPPGASNQDMMEEMDKQT
EECCCCCCCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MERGQMDQSQMEAGQMIRGADAVIKAIAAHGVTTVFGYPGGAIMPIYDALYGSPVEHLLS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCHHHHHHH
RHEQGAAFAAVGYARASGQTGVCFATSGPGATNLVTSLADALLDSVPVVAITGQVSTAVI
CCCCCCEEEEEHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCEEEEE
GTDAFQEVDVLGMSLSCTKHSFMVTHVDELVPTLYKAFEIAASGRPGPVLVDIPKDVQIA
CCCHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEE
MLEYKTPLQAVTQEPEHTPEQINDARELISQAKQPMLYVGGGVGMASAVDQLRDFIKVTG
EEECCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCC
IPSVATLKGLGSITIDTPGYLGMLGMHGSKAANLAVQECDLLIVVGARFDDRVTGRLASF
CCCHHHHHCCCEEEECCCCCEEEECCCCCHHHHEEHCCCCEEEEECCCCCCHHHHHHHHH
AENAKVIHLDIDAAELGKLRLPEVAIAGDLRQILPALTQNLGEQFAPWQAKVAQMKQEFQ
HCCCEEEEEECCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
WRYDRPGELIYAPAMLNRLAHKLPQDSVVACDVGQHQMWVAQHMFFNRPEDHLSSAGLGT
CCCCCCCCEEECHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHCCCCCC
MGFGLPAAIGAKVARPDACVVVVSGDGSFMMNVQELTTIKRKRLPVKILLVDNQKLGMVK
CCCCCHHHHCCCCCCCCEEEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCCHHH
QWQQLFFEERYSETDLSDNPDFVKMASAFDIPGRNIYKACDVEAGLDEMLNSQGPFLLHV
HHHHHHHHHHCCCCCCCCCCCCEEHHHHCCCCCCCHHHHCCCCCCHHHHHCCCCCEEEEE
SIDDAHNVWPLVPPGASNQDMMEEMDKQT
EECCCCCCCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3550695; 1379743; 9278503; 1995430; 6154938; 7015336; 3897211 [H]