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The map label for this gene is comM [H]

Identifier: 157960161

GI number: 157960161

Start: 372147

End: 373667

Strand: Reverse

Name: comM [H]

Synonym: Spea_0332

Alternate gene names: 157960161

Gene position: 373667-372147 (Counterclockwise)

Preceding gene: 157960163

Following gene: 157960160

Centisome position: 7.22

GC content: 52.07

Gene sequence:

>1521_bases
ATGCCCATAGCCAGTATTGCCACCCGTGCCAGCTTTGGCGTCCAAGCCCCACCTGTCACCGTCGAAGTCCACCTCAGCAA
CGGACTGCCCGCATTCAACCTAGTAGGACTGCCCGAAGCCTCAGTCAAAGAGGCCAAAGAACGTGTGAGAAGCGCACTCA
TCAACGCCGGATTTGAATTCCCCATGCGCCGCATCACAGTTAACCTAGCGCCAGCGGATCTCCCCAAACAAGGTGGCCGC
TATGATCTACCTATCGCCATCGGTATCCTCGCCGCCTCGAAACAGATCCCTGCAAAATCTGTCGAGCAACATGAATTCGT
CGGCGAACTAGCGCTAACCGGCCATATCCGTTACTGCCAAGGCCTACTGCCTGTGATAGTCGAAGCCAGCAAGCAAAATA
GCACCTTAGTCTTGCCGATTGATAATCGTGAAGATGCCGAGCTGGTCGGTTTCGAGAAAGTCTGCTTCGCTACCCATCTG
CAATCACTGGCTGCGTATCTGCACGGCCAATCGCAACTGCCGAGTATCGATCTTAACCTCGAATGGCTAACACAGAGTTC
AGAGCCTAAAACCTCCTGTTTAAGTGAGGTTATAGGCCAATATCAGGCTAAACAAGCGTTAGAGATTGCCGCTGCGGGTA
ACCATAATTTGTTGTTACTAGGGCCTCCCGGCACAGGCAAGACCATGCTCGCCAGCCGCATGATGCAGCTATTACCTAAA
TTGAACTACGACGAAGCACTGGAAGTTGCCGCCATTCACTCCGTCGCGGGTAACAACATCAAACCCGATAGCTTCTATCG
CCGCCCCTTTAGAAGCCCTCACCATACCTGTTCGGCGGTGTCTCTGGTGGGTGGCGGTGCCCAGCCTAAACCCGGTGAAA
TATCATTAGCCCATAGAGGCGTTCTGTTTTTAGATGAGGTAGCTGAGTTTCCTAGAAAAGTACTCGATTGCCTACGTGAG
CCAATGGAAACAGGTGAAGTGAGTATCTCGCGCGCGGCTGCAAAGCTGACCTTTGCCAGTCGATTTCAGTTAATCGCTGC
CATGAATCCTAGCCCCTGTGGTGATACCGATAATGCTAGGGCTAGTAATGAGCAGATCCAACGCTATCTTTCAAAGTTGT
CTGGGCCGTTTCTCGACCGCTTCGACCTCACCATAGATGTGCCTAAACTGCCTTTAGGCTCTCTTAACAGTCAAAACACT
CAAGCCGAAACCAGTGAAACAATAGCCAAACGGGTACAACAAGCACGAAAAACGCAACTCGCTCGCTCTGGTGTGCTCAA
CAGTGAACTCACGGGCAAGCAACTCAAACAAGATGCTAGGTTTCGCGATGATGACTTAGTCTTTTTAGAGCAAAGCGTAC
ATAGGCTCGGCTTATCGGTGCGTAGTTATCACCGCCTACAGCGGGTGGCCCGCACCATTGCCGACCTTCAACAGGAAGAT
AACGTCAGCCGCGCCCATATCGCCCAGGCGTTAGGGTACCGCGCCATGGATAGATTACTTGCCTCACTGTCTAGCCAATA
A

Upstream 100 bases:

>100_bases
AAACCCCATATAACTGCTAAACTTAACCAGAACCAAAACAAAGGGAAAATTGGATTCCCCTATCCAACAAAGTAACCTCA
TAAAACAAAAGGATTTGTTT

Downstream 100 bases:

>100_bases
TGACTATGCAGTTCATTTGCAGCAGCCGAGATATCTTAAAATCGAATATCTATTAGTCATGACACAAATAAGTTGAATCC
GACGCCAGTTTGCAGTAAAT

Product: Mg chelatase subunit ChlI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MPIASIATRASFGVQAPPVTVEVHLSNGLPAFNLVGLPEASVKEAKERVRSALINAGFEFPMRRITVNLAPADLPKQGGR
YDLPIAIGILAASKQIPAKSVEQHEFVGELALTGHIRYCQGLLPVIVEASKQNSTLVLPIDNREDAELVGFEKVCFATHL
QSLAAYLHGQSQLPSIDLNLEWLTQSSEPKTSCLSEVIGQYQAKQALEIAAAGNHNLLLLGPPGTGKTMLASRMMQLLPK
LNYDEALEVAAIHSVAGNNIKPDSFYRRPFRSPHHTCSAVSLVGGGAQPKPGEISLAHRGVLFLDEVAEFPRKVLDCLRE
PMETGEVSISRAAAKLTFASRFQLIAAMNPSPCGDTDNARASNEQIQRYLSKLSGPFLDRFDLTIDVPKLPLGSLNSQNT
QAETSETIAKRVQQARKTQLARSGVLNSELTGKQLKQDARFRDDDLVFLEQSVHRLGLSVRSYHRLQRVARTIADLQQED
NVSRAHIAQALGYRAMDRLLASLSSQ

Sequences:

>Translated_506_residues
MPIASIATRASFGVQAPPVTVEVHLSNGLPAFNLVGLPEASVKEAKERVRSALINAGFEFPMRRITVNLAPADLPKQGGR
YDLPIAIGILAASKQIPAKSVEQHEFVGELALTGHIRYCQGLLPVIVEASKQNSTLVLPIDNREDAELVGFEKVCFATHL
QSLAAYLHGQSQLPSIDLNLEWLTQSSEPKTSCLSEVIGQYQAKQALEIAAAGNHNLLLLGPPGTGKTMLASRMMQLLPK
LNYDEALEVAAIHSVAGNNIKPDSFYRRPFRSPHHTCSAVSLVGGGAQPKPGEISLAHRGVLFLDEVAEFPRKVLDCLRE
PMETGEVSISRAAAKLTFASRFQLIAAMNPSPCGDTDNARASNEQIQRYLSKLSGPFLDRFDLTIDVPKLPLGSLNSQNT
QAETSETIAKRVQQARKTQLARSGVLNSELTGKQLKQDARFRDDDLVFLEQSVHRLGLSVRSYHRLQRVARTIADLQQED
NVSRAHIAQALGYRAMDRLLASLSSQ
>Mature_505_residues
PIASIATRASFGVQAPPVTVEVHLSNGLPAFNLVGLPEASVKEAKERVRSALINAGFEFPMRRITVNLAPADLPKQGGRY
DLPIAIGILAASKQIPAKSVEQHEFVGELALTGHIRYCQGLLPVIVEASKQNSTLVLPIDNREDAELVGFEKVCFATHLQ
SLAAYLHGQSQLPSIDLNLEWLTQSSEPKTSCLSEVIGQYQAKQALEIAAAGNHNLLLLGPPGTGKTMLASRMMQLLPKL
NYDEALEVAAIHSVAGNNIKPDSFYRRPFRSPHHTCSAVSLVGGGAQPKPGEISLAHRGVLFLDEVAEFPRKVLDCLREP
METGEVSISRAAAKLTFASRFQLIAAMNPSPCGDTDNARASNEQIQRYLSKLSGPFLDRFDLTIDVPKLPLGSLNSQNTQ
AETSETIAKRVQQARKTQLARSGVLNSELTGKQLKQDARFRDDDLVFLEQSVHRLGLSVRSYHRLQRVARTIADLQQEDN
VSRAHIAQALGYRAMDRLLASLSSQ

Specific function: Induced during competence development. Not needed for DNA uptake. May be involved in a recombination step [H]

COG id: COG0606

COG function: function code O; Predicted ATPase with chaperone activity

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Mg-chelatase subunits D/I family. ComM subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693202, Length=512, Percent_Identity=50.1953125, Blast_Score=515, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001208
- InterPro:   IPR004482
- InterPro:   IPR000523
- InterPro:   IPR020568 [H]

Pfam domain/function: PF01078 Mg_chelatase [H]

EC number: NA

Molecular weight: Translated: 55195; Mature: 55064

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS50051 MCM_2 ; PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIASIATRASFGVQAPPVTVEVHLSNGLPAFNLVGLPEASVKEAKERVRSALINAGFEF
CCCHHHHHHHHCCCCCCCEEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCC
PMRRITVNLAPADLPKQGGRYDLPIAIGILAASKQIPAKSVEQHEFVGELALTGHIRYCQ
CEEEEEEEECCCCCCCCCCCCCCCHHEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GLLPVIVEASKQNSTLVLPIDNREDAELVGFEKVCFATHLQSLAAYLHGQSQLPSIDLNL
HHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCEE
EWLTQSSEPKTSCLSEVIGQYQAKQALEIAAAGNHNLLLLGPPGTGKTMLASRMMQLLPK
EEECCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCHHHHHHHHHHHHCC
LNYDEALEVAAIHSVAGNNIKPDSFYRRPFRSPHHTCSAVSLVGGGAQPKPGEISLAHRG
CCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHEECCCCCCCCCCEEEHHCC
VLFLDEVAEFPRKVLDCLREPMETGEVSISRAAAKLTFASRFQLIAAMNPSPCGDTDNAR
HHHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCC
ASNEQIQRYLSKLSGPFLDRFDLTIDVPKLPLGSLNSQNTQAETSETIAKRVQQARKTQL
CCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ARSGVLNSELTGKQLKQDARFRDDDLVFLEQSVHRLGLSVRSYHRLQRVARTIADLQQED
HHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NVSRAHIAQALGYRAMDRLLASLSSQ
CCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PIASIATRASFGVQAPPVTVEVHLSNGLPAFNLVGLPEASVKEAKERVRSALINAGFEF
CCHHHHHHHHCCCCCCCEEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCC
PMRRITVNLAPADLPKQGGRYDLPIAIGILAASKQIPAKSVEQHEFVGELALTGHIRYCQ
CEEEEEEEECCCCCCCCCCCCCCCHHEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GLLPVIVEASKQNSTLVLPIDNREDAELVGFEKVCFATHLQSLAAYLHGQSQLPSIDLNL
HHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCEE
EWLTQSSEPKTSCLSEVIGQYQAKQALEIAAAGNHNLLLLGPPGTGKTMLASRMMQLLPK
EEECCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCHHHHHHHHHHHHCC
LNYDEALEVAAIHSVAGNNIKPDSFYRRPFRSPHHTCSAVSLVGGGAQPKPGEISLAHRG
CCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHEECCCCCCCCCCEEEHHCC
VLFLDEVAEFPRKVLDCLREPMETGEVSISRAAAKLTFASRFQLIAAMNPSPCGDTDNAR
HHHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCC
ASNEQIQRYLSKLSGPFLDRFDLTIDVPKLPLGSLNSQNTQAETSETIAKRVQQARKTQL
CCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ARSGVLNSELTGKQLKQDARFRDDDLVFLEQSVHRLGLSVRSYHRLQRVARTIADLQQED
HHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NVSRAHIAQALGYRAMDRLLASLSSQ
CCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800; 9457884 [H]