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The map label for this gene is ilvK [H]

Identifier: 157960156

GI number: 157960156

Start: 366353

End: 367444

Strand: Direct

Name: ilvK [H]

Synonym: Spea_0327

Alternate gene names: 157960156

Gene position: 366353-367444 (Clockwise)

Preceding gene: 157960155

Following gene: 157960162

Centisome position: 7.08

GC content: 48.44

Gene sequence:

>1092_bases
ATGCAAATAACTTATAATTTAAAACCTGCGTTAGAGCGCCGTACCGAGCAATTTAAGCCGACAACTAATGTAGGCTTCGG
TAACCTCAGAACTGACCACATGTTTTTAATGGATTATCGCGACGGCGAGTGGTGTGATCCAAGAGTCGTTCCCTATGGCC
CATTTCAAGTCGCACCGGGTGCCATCGCTCTACATTATGGTCAGTCTGTATTTGAGGGCGCAAAAGCCTTTCAACATGAC
GACGGTGAGATCTACACCTTCCGTATCGATAAAAATGCCGAGCGCTTAAATCGCTCTGCTGACATTTTGTGTATTCCAGC
GCTGCCTGAGCAGATGCAGGTTGATGCCATCAATGCCTTGATTGATGTCGATCGTCTATGGTTTCCGATGCAGGAAGGGG
CTTGCCTGTATATTCGTCCGTTTATCTTTGCCACAGAAGATCGTCTATCGGTTAGTCCGAGCGAGCAATACACTTTCTGC
GTAATATTAAGCCCAAGCGGGCCTTATTACAGCGATGGCTTCGATAAGGCGATCCGTTTATTGATCAGTGAGCGTTTCCA
CCGTGCAGTTTCGGGTGGTACTGGCGCATCGAAGGCTGCGGGTAACTACGCGGCATCACTAAAAGCGGGTAAGGCGGCTG
CTGAATATGGCGCCGCGCAGGTACTTTACCTAGATGCTAACAATAAGCAGATTGAAGAAGTGGGCGCGATGAACCATTTT
CACGTGCTTAAAGATGGCACCATTATTATCCCTAAGTTCACCGACACCATCTTAAAGTCGATTACCTCACAGTCTATTCT
CGATTTGGGTGAGTTACTGGGCTGCGAAGTACGCCAAGAGACAGTGATGCTGGAGCAGTTTATTAGCGACATAGAGTCGG
GTGAGATTATCGAGGCGGGCGGTTTTGGTACTGCGGCCGTGGTATCGCCAGTGGGTTCTTATATCTTTGAAGATAACCGC
ATCGTAACCGTTGGAAACGGTGAAGTGGGTGAGCACACTAAGCGAATTTACAAGGTATTGACCGATATTCAGAAGGGGCT
TATTCAAGGTCCTGCAGGCTGGGTACAGTACGTTAAGCGTGTGCAAGCTTAG

Upstream 100 bases:

>100_bases
CCGTTCTTGGTTATTGAAGAACTTGAAGCTAGCCCCCGTTTTGCTTGACTTTTTTATGACCACTGATAGGCTGCTCAGTC
AGTAATCAAGGTGGTATACA

Downstream 100 bases:

>100_bases
CCTTCACTTAGCTGACCAAACCCTGCAGAAGGCTCCTTATGGGGCCTTTTTTATGGGGGGCTCTTTTTAAGCGCCACTCT
TTTACAGTGCAGCTCTTTTA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT 2; Vegetative protein 85; VEG85 [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MQITYNLKPALERRTEQFKPTTNVGFGNLRTDHMFLMDYRDGEWCDPRVVPYGPFQVAPGAIALHYGQSVFEGAKAFQHD
DGEIYTFRIDKNAERLNRSADILCIPALPEQMQVDAINALIDVDRLWFPMQEGACLYIRPFIFATEDRLSVSPSEQYTFC
VILSPSGPYYSDGFDKAIRLLISERFHRAVSGGTGASKAAGNYAASLKAGKAAAEYGAAQVLYLDANNKQIEEVGAMNHF
HVLKDGTIIIPKFTDTILKSITSQSILDLGELLGCEVRQETVMLEQFISDIESGEIIEAGGFGTAAVVSPVGSYIFEDNR
IVTVGNGEVGEHTKRIYKVLTDIQKGLIQGPAGWVQYVKRVQA

Sequences:

>Translated_363_residues
MQITYNLKPALERRTEQFKPTTNVGFGNLRTDHMFLMDYRDGEWCDPRVVPYGPFQVAPGAIALHYGQSVFEGAKAFQHD
DGEIYTFRIDKNAERLNRSADILCIPALPEQMQVDAINALIDVDRLWFPMQEGACLYIRPFIFATEDRLSVSPSEQYTFC
VILSPSGPYYSDGFDKAIRLLISERFHRAVSGGTGASKAAGNYAASLKAGKAAAEYGAAQVLYLDANNKQIEEVGAMNHF
HVLKDGTIIIPKFTDTILKSITSQSILDLGELLGCEVRQETVMLEQFISDIESGEIIEAGGFGTAAVVSPVGSYIFEDNR
IVTVGNGEVGEHTKRIYKVLTDIQKGLIQGPAGWVQYVKRVQA
>Mature_363_residues
MQITYNLKPALERRTEQFKPTTNVGFGNLRTDHMFLMDYRDGEWCDPRVVPYGPFQVAPGAIALHYGQSVFEGAKAFQHD
DGEIYTFRIDKNAERLNRSADILCIPALPEQMQVDAINALIDVDRLWFPMQEGACLYIRPFIFATEDRLSVSPSEQYTFC
VILSPSGPYYSDGFDKAIRLLISERFHRAVSGGTGASKAAGNYAASLKAGKAAAEYGAAQVLYLDANNKQIEEVGAMNHF
HVLKDGTIIIPKFTDTILKSITSQSILDLGELLGCEVRQETVMLEQFISDIESGEIIEAGGFGTAAVVSPVGSYIFEDNR
IVTVGNGEVGEHTKRIYKVLTDIQKGLIQGPAGWVQYVKRVQA

Specific function: Transaminates branched-chain amino acids and ketoglutarate [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI50658084, Length=335, Percent_Identity=34.6268656716418, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI296010904, Length=332, Percent_Identity=35.5421686746988, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI296010906, Length=332, Percent_Identity=35.5421686746988, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI38176287, Length=332, Percent_Identity=35.5421686746988, Blast_Score=188, Evalue=6e-48,
Organism=Homo sapiens, GI258614015, Length=286, Percent_Identity=34.965034965035, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI296010902, Length=332, Percent_Identity=31.6265060240964, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI296010900, Length=332, Percent_Identity=31.6265060240964, Blast_Score=149, Evalue=4e-36,
Organism=Escherichia coli, GI48994963, Length=321, Percent_Identity=31.1526479750779, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17568601, Length=359, Percent_Identity=33.7047353760446, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17565728, Length=341, Percent_Identity=33.4310850439883, Blast_Score=169, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6322002, Length=361, Percent_Identity=32.9639889196676, Blast_Score=185, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6322608, Length=340, Percent_Identity=34.1176470588235, Blast_Score=182, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24641779, Length=349, Percent_Identity=34.6704871060172, Blast_Score=184, Evalue=1e-46,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 40136; Mature: 40136

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITYNLKPALERRTEQFKPTTNVGFGNLRTDHMFLMDYRDGEWCDPRVVPYGPFQVAPG
CEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCC
AIALHYGQSVFEGAKAFQHDDGEIYTFRIDKNAERLNRSADILCIPALPEQMQVDAINAL
EEEEECCHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCCCCEEEECCCCCHHHHHHHHHH
IDVDRLWFPMQEGACLYIRPFIFATEDRLSVSPSEQYTFCVILSPSGPYYSDGFDKAIRL
HHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHH
LISERFHRAVSGGTGASKAAGNYAASLKAGKAAAEYGAAQVLYLDANNKQIEEVGAMNHF
HHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHCCCEEEEEEECCCCHHHHHCCCCCE
HVLKDGTIIIPKFTDTILKSITSQSILDLGELLGCEVRQETVMLEQFISDIESGEIIEAG
EEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEC
GFGTAAVVSPVGSYIFEDNRIVTVGNGEVGEHTKRIYKVLTDIQKGLIQGPAGWVQYVKR
CCCHHHHHHHHHHHEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VQA
HCC
>Mature Secondary Structure
MQITYNLKPALERRTEQFKPTTNVGFGNLRTDHMFLMDYRDGEWCDPRVVPYGPFQVAPG
CEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCC
AIALHYGQSVFEGAKAFQHDDGEIYTFRIDKNAERLNRSADILCIPALPEQMQVDAINAL
EEEEECCHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCCCCEEEECCCCCHHHHHHHHHH
IDVDRLWFPMQEGACLYIRPFIFATEDRLSVSPSEQYTFCVILSPSGPYYSDGFDKAIRL
HHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHH
LISERFHRAVSGGTGASKAAGNYAASLKAGKAAAEYGAAQVLYLDANNKQIEEVGAMNHF
HHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHCCCEEEEEEECCCCHHHHHCCCCCE
HVLKDGTIIIPKFTDTILKSITSQSILDLGELLGCEVRQETVMLEQFISDIESGEIIEAG
EEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEC
GFGTAAVVSPVGSYIFEDNRIVTVGNGEVGEHTKRIYKVLTDIQKGLIQGPAGWVQYVKR
CCCHHHHHHHHHHHEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VQA
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 7934828; 9298659 [H]