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The map label for this gene is htrB [H]

Identifier: 157960110

GI number: 157960110

Start: 314232

End: 315161

Strand: Reverse

Name: htrB [H]

Synonym: Spea_0281

Alternate gene names: 157960110

Gene position: 315161-314232 (Counterclockwise)

Preceding gene: 157960112

Following gene: 157960106

Centisome position: 6.09

GC content: 44.95

Gene sequence:

>930_bases
ATGAGTAAGTACACAGTTCCAACCTTCTCACTAGCAATGCTACATCCCAAATATTGGTGTGTTTTATTAATTATTAGCAT
TAGCTACCTTTGCAGCCTATTACCATACGCGATTCAAATTCGTTTAGGTAAGCTAGTCGGCCGCGTCGTACTTAGCTTTA
TGAAGCGTCGCACACGCACAATTAAGCGCAATCTTGAGCTGTGTTTTCCAGACATGACTGACGCCGAGCGTGAAAAGTTA
ACCAAACAGAATATTGAGAATACCGGATTAGCCCTATTCGAAACGGGCATGGCTTGGTTTTGGTCTAATGCGCGTATTGC
CAAGCACACCAGCGTAGTTGGCATAGAGCATATCGAAAAGCTTAAAACCGAAGGTCGTGGCGCATTGCTGATTGCAGTGC
ATTCACTCAACCTTGAGTTAAGCGGTCGTGCCTTTGGCCAATATGCGCAGGGCATGGCAGTATACCGCCCAAACAACAAT
CCTTGCTTTGACTACTTCCAATTAAAGGGCCGTTCTCAAGGTGGTCATACCTTGATCCATCGTAAGAACGTTAAGTTTAT
GCTGGAGGCACTCAATAATGGCGCTTTTATGTGGTACGCACCGGATCATGATTACGGTCGTCGCCGCTCTACTTTCGCGC
CACTATTTGCCGTAGAAAATGCTTGTACTACAACGGGAACAAGTCTGTTAGCAGACAATACTAACTGCGCCATAGTACCG
GTGGTGTTTGTACGTGATAACGATAGTGGCCACTACACCTTAACTATTAAAGAGCCAATGATAGATAAGTTTCCCAAGGG
AGATGAACACGCAGGAGCCTGCTTTATCAATAAGATTGTTGAAGAGGCTATCATGGCTAAGCCAAGCCAATATATGTGGT
TACATAGACGCTTTAAGAACCGCCCAGATGGTGAAAGCTCTCTGTATTAA

Upstream 100 bases:

>100_bases
AGGCTTTAGATTAATTAACTAATTATACATAGCTATAAAGTGAAAAATATCACGTATAATCCCGCGACATTTTTCAGTAT
CACTAAAAGCTCAATCCCAC

Downstream 100 bases:

>100_bases
TGCTTTAAAAGCGATTCTAAAATCTAGCCTTTAACCTACTAATCAAACCAGGCTGCTGTTTTCTCGGCGGCTTGGTTTTT
TACGTTATCATCGACATACA

Product: lipid A biosynthesis lauroyl (or palmitoleoyl) acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MSKYTVPTFSLAMLHPKYWCVLLIISISYLCSLLPYAIQIRLGKLVGRVVLSFMKRRTRTIKRNLELCFPDMTDAEREKL
TKQNIENTGLALFETGMAWFWSNARIAKHTSVVGIEHIEKLKTEGRGALLIAVHSLNLELSGRAFGQYAQGMAVYRPNNN
PCFDYFQLKGRSQGGHTLIHRKNVKFMLEALNNGAFMWYAPDHDYGRRRSTFAPLFAVENACTTTGTSLLADNTNCAIVP
VVFVRDNDSGHYTLTIKEPMIDKFPKGDEHAGACFINKIVEEAIMAKPSQYMWLHRRFKNRPDGESSLY

Sequences:

>Translated_309_residues
MSKYTVPTFSLAMLHPKYWCVLLIISISYLCSLLPYAIQIRLGKLVGRVVLSFMKRRTRTIKRNLELCFPDMTDAEREKL
TKQNIENTGLALFETGMAWFWSNARIAKHTSVVGIEHIEKLKTEGRGALLIAVHSLNLELSGRAFGQYAQGMAVYRPNNN
PCFDYFQLKGRSQGGHTLIHRKNVKFMLEALNNGAFMWYAPDHDYGRRRSTFAPLFAVENACTTTGTSLLADNTNCAIVP
VVFVRDNDSGHYTLTIKEPMIDKFPKGDEHAGACFINKIVEEAIMAKPSQYMWLHRRFKNRPDGESSLY
>Mature_308_residues
SKYTVPTFSLAMLHPKYWCVLLIISISYLCSLLPYAIQIRLGKLVGRVVLSFMKRRTRTIKRNLELCFPDMTDAEREKLT
KQNIENTGLALFETGMAWFWSNARIAKHTSVVGIEHIEKLKTEGRGALLIAVHSLNLELSGRAFGQYAQGMAVYRPNNNP
CFDYFQLKGRSQGGHTLIHRKNVKFMLEALNNGAFMWYAPDHDYGRRRSTFAPLFAVENACTTTGTSLLADNTNCAIVPV
VFVRDNDSGHYTLTIKEPMIDKFPKGDEHAGACFINKIVEEAIMAKPSQYMWLHRRFKNRPDGESSLY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=304, Percent_Identity=48.6842105263158, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI87082094, Length=301, Percent_Identity=42.8571428571429, Blast_Score=275, Evalue=2e-75,
Organism=Escherichia coli, GI1788161, Length=310, Percent_Identity=26.7741935483871, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35168; Mature: 35037

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKYTVPTFSLAMLHPKYWCVLLIISISYLCSLLPYAIQIRLGKLVGRVVLSFMKRRTRT
CCCCCCCCEEEEHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKRNLELCFPDMTDAEREKLTKQNIENTGLALFETGMAWFWSNARIAKHTSVVGIEHIEK
HHHCHHEECCCCCCHHHHHHHHHCCCCCCEEEEECCHHEEECCCEEEHHHHHHHHHHHHH
LKTEGRGALLIAVHSLNLELSGRAFGQYAQGMAVYRPNNNPCFDYFQLKGRSQGGHTLIH
HHCCCCCEEEEEEEEECEEECCCHHHHHHCCEEEECCCCCCCEEEEEEECCCCCCCEEEE
RKNVKFMLEALNNGAFMWYAPDHDYGRRRSTFAPLFAVENACTTTGTSLLADNTNCAIVP
ECCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCEEEECCCCEEEEE
VVFVRDNDSGHYTLTIKEPMIDKFPKGDEHAGACFINKIVEEAIMAKPSQYMWLHRRFKN
EEEEEECCCCEEEEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
RPDGESSLY
CCCCCCCCC
>Mature Secondary Structure 
SKYTVPTFSLAMLHPKYWCVLLIISISYLCSLLPYAIQIRLGKLVGRVVLSFMKRRTRT
CCCCCCCEEEEHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKRNLELCFPDMTDAEREKLTKQNIENTGLALFETGMAWFWSNARIAKHTSVVGIEHIEK
HHHCHHEECCCCCCHHHHHHHHHCCCCCCEEEEECCHHEEECCCEEEHHHHHHHHHHHHH
LKTEGRGALLIAVHSLNLELSGRAFGQYAQGMAVYRPNNNPCFDYFQLKGRSQGGHTLIH
HHCCCCCEEEEEEEEECEEECCCHHHHHHCCEEEECCCCCCCEEEEEEECCCCCCCEEEE
RKNVKFMLEALNNGAFMWYAPDHDYGRRRSTFAPLFAVENACTTTGTSLLADNTNCAIVP
ECCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCEEEECCCCEEEEE
VVFVRDNDSGHYTLTIKEPMIDKFPKGDEHAGACFINKIVEEAIMAKPSQYMWLHRRFKN
EEEEEECCCCEEEEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
RPDGESSLY
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]