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The map label for this gene is frmA [H]

Identifier: 157960098

GI number: 157960098

Start: 301818

End: 302948

Strand: Direct

Name: frmA [H]

Synonym: Spea_0269

Alternate gene names: 157960098

Gene position: 301818-302948 (Clockwise)

Preceding gene: 157960097

Following gene: 157960099

Centisome position: 5.83

GC content: 45.71

Gene sequence:

>1131_bases
ATGACAGCACAAACAATCAAATCAAAAGCCGCAGTTGCCTGGGCAGTTGGTCAACCGCTATCTATGGAAATCGTAGATGT
TATGCCGCCTCAAAAAGGCGAAGTACGTGTGAAAATGATCGCTACAGGCGTATGTCACACAGACGCATTTACTCTTTCTG
GTGACGATCCAGAAGGTATTTTCCCTTGTATTCTTGGTCACGAAGGTGGCGGTATCGTTGAGTCTATCGGTGAAGGCGTA
ACTAGCGTTAAAGTGGGTGACCACGTTATCCCTCTTTACACTCCTGAGTGCGGCGAATGTAAATTCTGTAAGTCTGGCAA
AACTAACCTTTGTCAGAAGATCCGTGAAACTCAAGGTAAAGGTTTGATGCCAGATGGCACCACTCGTTTCTCTAAAGATG
GTGTTGAAATTTACCACTACATGGGTACATCAACTTTCTCTGAATACACAGTATTACCTGAGATCTCACTAGCGAAAGTT
AACCCTGAAGCGCCACTTGAAGAAGTTTGTCTACTAGGTTGTGGTGTCACAACCGGTATGGGCGCGGTAATGAACACCGC
TAAAGTAGAAGAAGGCGCAACTGTTGCTATCTTCGGTATGGGGGGTATTGGTCTGTCAGCGGTTATTGGCGCGGCAATGG
CTAAAGCATCACGTATCATTGCTATCGACATTAACGAAAGCAAGTTTGACCTAGCGCGTAAGCTTGGTGCTACTGATTGC
ATCAACCCTAAAGACTACGACAAGCCAATCCAAGATGTGATTGTAGAGCTTACAGACGGCGGCGTAGATTACTCATTCGA
GTGTATCGGTAACGTACACGTTATGCGTAGTGCATTAGAGTGTTGTCATAAAGGTTGGGGTGAGTCAGTTGTTATTGGTG
TTGCTGGTGCAGGCCAAGAGATCTCAACTCGTCCATTCCAGTTAGTAACTGGTCGTGTATGGAAAGGTAGCGCGTTCGGT
GGTGTTAAAGGCCGCTCTGAACTACCTGAATACGTTGAGCGTTACCTAGCAGGTGAGTTCAAGTTGAGTGACTTTATCAC
TCACACTATGGCTCTTGAGCAAGTTAACGAAGCATTTGACCTAATGCACGAAGGTAAGAGTATTCGTACTGTTATCCACT
TCGACAAGTAA

Upstream 100 bases:

>100_bases
ATTGATGGGATTATCAATAATGAACAGGTAGTTATAATCTTTCCATACCCACTACGGTGACGCACCGTAATTTAAAGTTT
ATTAAAACTAGGAATACAAA

Downstream 100 bases:

>100_bases
CATCATTGTCTAAAATAACAAGCGCTCTCTGGCGCTTGTTATTTTTAAAGGCTTAATTTTTAAAGATAGAGCTTTAAAGG
TTGAGCTTTACAGAATTAGT

Product: S-(hydroxymethyl)glutathione dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTAQTIKSKAAVAWAVGQPLSMEIVDVMPPQKGEVRVKMIATGVCHTDAFTLSGDDPEGIFPCILGHEGGGIVESIGEGV
TSVKVGDHVIPLYTPECGECKFCKSGKTNLCQKIRETQGKGLMPDGTTRFSKDGVEIYHYMGTSTFSEYTVLPEISLAKV
NPEAPLEEVCLLGCGVTTGMGAVMNTAKVEEGATVAIFGMGGIGLSAVIGAAMAKASRIIAIDINESKFDLARKLGATDC
INPKDYDKPIQDVIVELTDGGVDYSFECIGNVHVMRSALECCHKGWGESVVIGVAGAGQEISTRPFQLVTGRVWKGSAFG
GVKGRSELPEYVERYLAGEFKLSDFITHTMALEQVNEAFDLMHEGKSIRTVIHFDK

Sequences:

>Translated_376_residues
MTAQTIKSKAAVAWAVGQPLSMEIVDVMPPQKGEVRVKMIATGVCHTDAFTLSGDDPEGIFPCILGHEGGGIVESIGEGV
TSVKVGDHVIPLYTPECGECKFCKSGKTNLCQKIRETQGKGLMPDGTTRFSKDGVEIYHYMGTSTFSEYTVLPEISLAKV
NPEAPLEEVCLLGCGVTTGMGAVMNTAKVEEGATVAIFGMGGIGLSAVIGAAMAKASRIIAIDINESKFDLARKLGATDC
INPKDYDKPIQDVIVELTDGGVDYSFECIGNVHVMRSALECCHKGWGESVVIGVAGAGQEISTRPFQLVTGRVWKGSAFG
GVKGRSELPEYVERYLAGEFKLSDFITHTMALEQVNEAFDLMHEGKSIRTVIHFDK
>Mature_375_residues
TAQTIKSKAAVAWAVGQPLSMEIVDVMPPQKGEVRVKMIATGVCHTDAFTLSGDDPEGIFPCILGHEGGGIVESIGEGVT
SVKVGDHVIPLYTPECGECKFCKSGKTNLCQKIRETQGKGLMPDGTTRFSKDGVEIYHYMGTSTFSEYTVLPEISLAKVN
PEAPLEEVCLLGCGVTTGMGAVMNTAKVEEGATVAIFGMGGIGLSAVIGAAMAKASRIIAIDINESKFDLARKLGATDCI
NPKDYDKPIQDVIVELTDGGVDYSFECIGNVHVMRSALECCHKGWGESVVIGVAGAGQEISTRPFQLVTGRVWKGSAFGG
VKGRSELPEYVERYLAGEFKLSDFITHTMALEQVNEAFDLMHEGKSIRTVIHFDK

Specific function: Has High Formaldehyde Dehydrogenase Activity In The Presence Of Glutathione And Catalyzes The Oxidation Of Normal Alcohols In A Reaction That Is Not Gsh-Dependent. In Addition, Hemithiolacetals Other Than Those Formed From Gsh, Including Omega-Thiol Fatty

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=372, Percent_Identity=65.0537634408602, Blast_Score=514, Evalue=1e-146,
Organism=Homo sapiens, GI34577061, Length=371, Percent_Identity=53.3692722371968, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI4501933, Length=371, Percent_Identity=51.2129380053908, Blast_Score=407, Evalue=1e-114,
Organism=Homo sapiens, GI4501929, Length=371, Percent_Identity=51.2129380053908, Blast_Score=407, Evalue=1e-114,
Organism=Homo sapiens, GI71565152, Length=375, Percent_Identity=51.7333333333333, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI156523966, Length=371, Percent_Identity=49.0566037735849, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI262073058, Length=372, Percent_Identity=49.1935483870968, Blast_Score=380, Evalue=1e-106,
Organism=Homo sapiens, GI71743840, Length=371, Percent_Identity=49.0566037735849, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI4501939, Length=365, Percent_Identity=49.3150684931507, Blast_Score=377, Evalue=1e-104,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=71.5447154471545, Blast_Score=542, Evalue=1e-155,
Organism=Escherichia coli, GI1790045, Length=362, Percent_Identity=26.5193370165746, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787863, Length=356, Percent_Identity=25.2808988764045, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87082125, Length=364, Percent_Identity=25.8241758241758, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI87081918, Length=348, Percent_Identity=29.3103448275862, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1788073, Length=351, Percent_Identity=26.2108262108262, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788407, Length=231, Percent_Identity=27.7056277056277, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1786825, Length=265, Percent_Identity=25.6603773584906, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786518, Length=368, Percent_Identity=23.3695652173913, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI226510992, Length=353, Percent_Identity=23.5127478753541, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25146526, Length=377, Percent_Identity=59.6816976127321, Blast_Score=489, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI71997431, Length=379, Percent_Identity=59.3667546174143, Blast_Score=485, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17565904, Length=376, Percent_Identity=55.0531914893617, Blast_Score=440, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17562584, Length=363, Percent_Identity=30.3030303030303, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71988145, Length=371, Percent_Identity=27.2237196765499, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17562582, Length=380, Percent_Identity=27.6315789473684, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320033, Length=377, Percent_Identity=58.3554376657825, Blast_Score=469, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6319621, Length=386, Percent_Identity=30.3108808290155, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6323099, Length=380, Percent_Identity=27.3684210526316, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6323729, Length=365, Percent_Identity=28.2191780821918, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324486, Length=364, Percent_Identity=25.5494505494505, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323961, Length=364, Percent_Identity=25.5494505494505, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322619, Length=362, Percent_Identity=26.7955801104972, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6319955, Length=362, Percent_Identity=26.7955801104972, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319257, Length=237, Percent_Identity=25.7383966244726, Blast_Score=63, Evalue=1e-10,
Organism=Drosophila melanogaster, GI17737895, Length=373, Percent_Identity=64.343163538874, Blast_Score=510, Evalue=1e-145,
Organism=Drosophila melanogaster, GI17137530, Length=347, Percent_Identity=27.3775216138329, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17737897, Length=239, Percent_Identity=28.0334728033473, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284; =1.1.1.1 [H]

Molecular weight: Translated: 40197; Mature: 40065

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQTIKSKAAVAWAVGQPLSMEIVDVMPPQKGEVRVKMIATGVCHTDAFTLSGDDPEGI
CCCHHHHHCCEEEEECCCCCCEEEEECCCCCCCCEEEEEEEECEEECCEEEECCCCCCCC
FPCILGHEGGGIVESIGEGVTSVKVGDHVIPLYTPECGECKFCKSGKTNLCQKIRETQGK
EEEEEECCCCCHHHHHCCCCEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
GLMPDGTTRFSKDGVEIYHYMGTSTFSEYTVLPEISLAKVNPEAPLEEVCLLGCGVTTGM
CCCCCCCCCCCCCCEEEEEEECCCCCCCEEECCCCEEEECCCCCCHHHHHHHHCCHHHHH
GAVMNTAKVEEGATVAIFGMGGIGLSAVIGAAMAKASRIIAIDINESKFDLARKLGATDC
HHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHCCCCCC
INPKDYDKPIQDVIVELTDGGVDYSFECIGNVHVMRSALECCHKGWGESVVIGVAGAGQE
CCCCCCCCCHHHHHEECCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCC
ISTRPFQLVTGRVWKGSAFGGVKGRSELPEYVERYLAGEFKLSDFITHTMALEQVNEAFD
CCCCCEEEEECEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LMHEGKSIRTVIHFDK
HHHCCCCEEEEEEECC
>Mature Secondary Structure 
TAQTIKSKAAVAWAVGQPLSMEIVDVMPPQKGEVRVKMIATGVCHTDAFTLSGDDPEGI
CCHHHHHCCEEEEECCCCCCEEEEECCCCCCCCEEEEEEEECEEECCEEEECCCCCCCC
FPCILGHEGGGIVESIGEGVTSVKVGDHVIPLYTPECGECKFCKSGKTNLCQKIRETQGK
EEEEEECCCCCHHHHHCCCCEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
GLMPDGTTRFSKDGVEIYHYMGTSTFSEYTVLPEISLAKVNPEAPLEEVCLLGCGVTTGM
CCCCCCCCCCCCCCEEEEEEECCCCCCCEEECCCCEEEECCCCCCHHHHHHHHCCHHHHH
GAVMNTAKVEEGATVAIFGMGGIGLSAVIGAAMAKASRIIAIDINESKFDLARKLGATDC
HHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHCCCCCC
INPKDYDKPIQDVIVELTDGGVDYSFECIGNVHVMRSALECCHKGWGESVVIGVAGAGQE
CCCCCCCCCHHHHHEECCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCC
ISTRPFQLVTGRVWKGSAFGGVKGRSELPEYVERYLAGEFKLSDFITHTMALEQVNEAFD
CCCCCEEEEECEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LMHEGKSIRTVIHFDK
HHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]