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The map label for this gene is gfa [H]

Identifier: 157960096

GI number: 157960096

Start: 300361

End: 300918

Strand: Direct

Name: gfa [H]

Synonym: Spea_0267

Alternate gene names: 157960096

Gene position: 300361-300918 (Clockwise)

Preceding gene: 157960094

Following gene: 157960097

Centisome position: 5.8

GC content: 47.49

Gene sequence:

>558_bases
ATGACAACGTTAATTCACCCACAAGTCGACAACGGCATTCCTGCAACGGCAGCTAGTTTTGCTGGCGGATCACTGCAATG
TAAATGTGCAAACAATCCAGTTGAAGTCACCATTGCAGCACAAACTGCTCATAACCATGTATGTGGCTGCAGTAAATGTT
GGAAGCCTGAAGGCGCGCTATTTGCACAAATTGCCGTAGTCGCAAAAGATAAGGTTAGCGTAACCGCTAATGCCGACAAG
CTACACATCATTGATGAGAGCGCTGCAATTCAACGTCACGCTTGTAAAGAGTGCGGCACTCATATGTATGGCCGCATCGA
AAACACGGGGCACCCTTTCTATGGCTTAGACTTTGTGCATACAGAGCTATCTAAGCAGCAAGGTTGGTCGGCGCCAGAGT
TCGCTGCGTTTGTATCTTCAATCATAGAGTCGGGTACTGCGCCTGAGGCTATGGCAGATGTTCGCCAAACTCTTAAGGAT
CTCGGTTTACCTCCTTATGACTGTTTATCACCAGCATTAATGGATGCAATCGCGACCCATGTTGCGGCTAACAAATAA

Upstream 100 bases:

>100_bases
ACCGATATCGCCAAGGCTTAAGTTCCCCCCCCCTGCGTTTAGTATGGAATTTAGTATGGGGTTGATATTTGAGTCTTGGC
CCATTTTAGGATCAATTATT

Downstream 100 bases:

>100_bases
CGCTGAAGAGTGAGCCGATACCGGCTTAAATAGCACTTATACCAGTAATGCAAAAAGCGCTTCGGCGCTTTTTTTACATC
TATAGCCTAGCAATATGGCT

Product: glutathione-dependent formaldehyde-activating protein

Products: NA

Alternate protein names: S-(hydroxymethyl)glutathione synthase [H]

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MTTLIHPQVDNGIPATAASFAGGSLQCKCANNPVEVTIAAQTAHNHVCGCSKCWKPEGALFAQIAVVAKDKVSVTANADK
LHIIDESAAIQRHACKECGTHMYGRIENTGHPFYGLDFVHTELSKQQGWSAPEFAAFVSSIIESGTAPEAMADVRQTLKD
LGLPPYDCLSPALMDAIATHVAANK

Sequences:

>Translated_185_residues
MTTLIHPQVDNGIPATAASFAGGSLQCKCANNPVEVTIAAQTAHNHVCGCSKCWKPEGALFAQIAVVAKDKVSVTANADK
LHIIDESAAIQRHACKECGTHMYGRIENTGHPFYGLDFVHTELSKQQGWSAPEFAAFVSSIIESGTAPEAMADVRQTLKD
LGLPPYDCLSPALMDAIATHVAANK
>Mature_184_residues
TTLIHPQVDNGIPATAASFAGGSLQCKCANNPVEVTIAAQTAHNHVCGCSKCWKPEGALFAQIAVVAKDKVSVTANADKL
HIIDESAAIQRHACKECGTHMYGRIENTGHPFYGLDFVHTELSKQQGWSAPEFAAFVSSIIESGTAPEAMADVRQTLKDL
GLPPYDCLSPALMDAIATHVAANK

Specific function: Catalyzes the condensation of formaldehyde and glutathione to S-hydroxymethylglutathione [H]

COG id: COG3791

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfa family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014185
- InterPro:   IPR006913
- InterPro:   IPR011057 [H]

Pfam domain/function: PF04828 GFA [H]

EC number: =4.4.1.22 [H]

Molecular weight: Translated: 19615; Mature: 19484

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLIHPQVDNGIPATAASFAGGSLQCKCANNPVEVTIAAQTAHNHVCGCSKCWKPEGAL
CCCCCCCCCCCCCCCCHHHCCCCCEEEEECCCCEEEEEEECCCCCCCCCCHHHCCCCCHH
FAQIAVVAKDKVSVTANADKLHIIDESAAIQRHACKECGTHMYGRIENTGHPFYGLDFVH
HHHHHEEECCCEEEEECCCEEEEECCHHHHHHHHHHHHCCHHEEEECCCCCCEECHHHHH
TELSKQQGWSAPEFAAFVSSIIESGTAPEAMADVRQTLKDLGLPPYDCLSPALMDAIATH
HHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VAANK
HHCCC
>Mature Secondary Structure 
TTLIHPQVDNGIPATAASFAGGSLQCKCANNPVEVTIAAQTAHNHVCGCSKCWKPEGAL
CCCCCCCCCCCCCCCHHHCCCCCEEEEECCCCEEEEEEECCCCCCCCCCHHHCCCCCHH
FAQIAVVAKDKVSVTANADKLHIIDESAAIQRHACKECGTHMYGRIENTGHPFYGLDFVH
HHHHHEEECCCEEEEECCCEEEEECCHHHHHHHHHHHHCCHHEEEECCCCCCEECHHHHH
TELSKQQGWSAPEFAAFVSSIIESGTAPEAMADVRQTLKDLGLPPYDCLSPALMDAIATH
HHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VAANK
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]