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The map label for this gene is dosC [H]

Identifier: 157960094

GI number: 157960094

Start: 297718

End: 299088

Strand: Direct

Name: dosC [H]

Synonym: Spea_0265

Alternate gene names: 157960094

Gene position: 297718-299088 (Clockwise)

Preceding gene: 157960092

Following gene: 157960096

Centisome position: 5.75

GC content: 42.01

Gene sequence:

>1371_bases
ATGCTAGAAAGTAAAGTAAGCGAAAACTCTTGGTTATTAGTTAAGTATTCTCAAGCTATGATTCGGCCCTTATCGGCTGT
CGGTATTATTTTTGGCTGTGCGGCAATGTTTGGCTATTTGGGCGCTATGGAATGGATATATAGACCTGTCACTAATGGAC
CTGCGACGAACCCATTAACTGCAATGTGTATGATTTTTATAGGAACCGCTCTTTGGATAAGTAAAAAGAAAAGTGATGCT
AACTTGGCGCTGTTGTTATTATCGGCAGTTATCATTATTACATTGACCCTTATTTTTGATGTAATTTCTGGCTCAAACTT
CTCAGCGATACTCACGCCTTTTCAGGAACAAGTCGCGCTAGAATTAAAAAATGGCAAAAGTAATAGCATGGGGATTAACT
CCTCTGTCATGTTTCTTTTTATTGCTATTAGCTTGGTATTGTTTGTTGGGCAAAAATTAATTGCTTCGCAGTTGTTTGCC
TTCATTGCTTTAGCCATACCCACGGTTTCTTTTACTGGTTATGCCTACGGGCTTGAACAGTTTTACGGCCAAATGTCGAT
GTTGACGGCATTTATAGGGTTTGTTCTGGCCATAGCGGCATTAATGATGACGGCTAAAGTTGGTGGTGTGAACGCAATCT
TAAGTCCTTTTATTGGCGGGAAAATAGCACGTCGACAAACCGCTGCAGGTTATTTCGTCCCAGCAATACTTGGTTATTTG
TTGATTAAATCGCTTTGCGCAATAGATGGGGAACTATGTGGCATTTTTGTCGTAACTATCTGTTGGTTTGTCATACTCAT
GATAAGTTATTCAGCTCTTTTTCAAGAGAAGATGGATCTTGAACGTCGACAAATGGAACAGCTACTGACCTTAGCTGCAA
TGAACGACCAATTAACCGGGCTGGCAAACCGTAGAAAGTTCCATGAATTTGCGCAAGAGGAGTTAAACCGTGTACAGCGT
AACAAAAGCCAGTTTTGGGTTTTGGTTATGGACGTGGATTTCTTTAAAAAGATTAATGATACCGCTGGCCATGATATGGG
AGACCAAGTGCTTATTGAATTGAGCAAGGTGTTAAAAAATTCAGTTCGTGCCGTAGATCTGGTGAGTCGAATGGGGGGGG
AAGAGTTTTCCATCATTCTGAGCGACACGACAAAGCAGGGAGCTGAACGCGTGGCTGAGAGTATTCGAAGTAGCATTGAA
AGCATCAAGATTAAGGGCTGGACAGATATATATGGACCCATTACGGCTTCTATTGGCGGCGCTACGAGTGATGGGGCCGA
TAAGCTTGAAAATACGCTGAAGATTGCAGATAGTGCGTTATATCAAGCAAAACAGCGTGGTCGTAATCAGGTCTACTTTG
CTGCTGAGTGA

Upstream 100 bases:

>100_bases
TTCCCTATCTAGAGAACAGCAATTATTATAGTCAGTTATGTTGTTTTAACCTGTTAAAACAAGGCTAATTAGTTGTATTG
GAACGTGAAATAAATCACTT

Downstream 100 bases:

>100_bases
TAAGCATATCACTCAGCAGGCCAAAGCGAGTGGGGCCTAGTCTCTATTTTAGGCCTTCACTCAGGAAATCCAGCAGCATG
CGTACCTTAGGCGAAAGGTG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MLESKVSENSWLLVKYSQAMIRPLSAVGIIFGCAAMFGYLGAMEWIYRPVTNGPATNPLTAMCMIFIGTALWISKKKSDA
NLALLLLSAVIIITLTLIFDVISGSNFSAILTPFQEQVALELKNGKSNSMGINSSVMFLFIAISLVLFVGQKLIASQLFA
FIALAIPTVSFTGYAYGLEQFYGQMSMLTAFIGFVLAIAALMMTAKVGGVNAILSPFIGGKIARRQTAAGYFVPAILGYL
LIKSLCAIDGELCGIFVVTICWFVILMISYSALFQEKMDLERRQMEQLLTLAAMNDQLTGLANRRKFHEFAQEELNRVQR
NKSQFWVLVMDVDFFKKINDTAGHDMGDQVLIELSKVLKNSVRAVDLVSRMGGEEFSIILSDTTKQGAERVAESIRSSIE
SIKIKGWTDIYGPITASIGGATSDGADKLENTLKIADSALYQAKQRGRNQVYFAAE

Sequences:

>Translated_456_residues
MLESKVSENSWLLVKYSQAMIRPLSAVGIIFGCAAMFGYLGAMEWIYRPVTNGPATNPLTAMCMIFIGTALWISKKKSDA
NLALLLLSAVIIITLTLIFDVISGSNFSAILTPFQEQVALELKNGKSNSMGINSSVMFLFIAISLVLFVGQKLIASQLFA
FIALAIPTVSFTGYAYGLEQFYGQMSMLTAFIGFVLAIAALMMTAKVGGVNAILSPFIGGKIARRQTAAGYFVPAILGYL
LIKSLCAIDGELCGIFVVTICWFVILMISYSALFQEKMDLERRQMEQLLTLAAMNDQLTGLANRRKFHEFAQEELNRVQR
NKSQFWVLVMDVDFFKKINDTAGHDMGDQVLIELSKVLKNSVRAVDLVSRMGGEEFSIILSDTTKQGAERVAESIRSSIE
SIKIKGWTDIYGPITASIGGATSDGADKLENTLKIADSALYQAKQRGRNQVYFAAE
>Mature_456_residues
MLESKVSENSWLLVKYSQAMIRPLSAVGIIFGCAAMFGYLGAMEWIYRPVTNGPATNPLTAMCMIFIGTALWISKKKSDA
NLALLLLSAVIIITLTLIFDVISGSNFSAILTPFQEQVALELKNGKSNSMGINSSVMFLFIAISLVLFVGQKLIASQLFA
FIALAIPTVSFTGYAYGLEQFYGQMSMLTAFIGFVLAIAALMMTAKVGGVNAILSPFIGGKIARRQTAAGYFVPAILGYL
LIKSLCAIDGELCGIFVVTICWFVILMISYSALFQEKMDLERRQMEQLLTLAAMNDQLTGLANRRKFHEFAQEELNRVQR
NKSQFWVLVMDVDFFKKINDTAGHDMGDQVLIELSKVLKNSVRAVDLVSRMGGEEFSIILSDTTKQGAERVAESIRSSIE
SIKIKGWTDIYGPITASIGGATSDGADKLENTLKIADSALYQAKQRGRNQVYFAAE

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=41.6666666666667, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87081881, Length=171, Percent_Identity=38.5964912280702, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=38.0368098159509, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1786584, Length=233, Percent_Identity=28.755364806867, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87082007, Length=196, Percent_Identity=34.6938775510204, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1788085, Length=170, Percent_Identity=31.7647058823529, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788381, Length=172, Percent_Identity=31.9767441860465, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1787541, Length=170, Percent_Identity=30.5882352941176, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081974, Length=191, Percent_Identity=29.8429319371728, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787816, Length=169, Percent_Identity=33.1360946745562, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787802, Length=175, Percent_Identity=30.2857142857143, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788956, Length=190, Percent_Identity=31.5789473684211, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787056, Length=163, Percent_Identity=32.5153374233129, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87081977, Length=173, Percent_Identity=28.3236994219653, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 50006; Mature: 50006

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLESKVSENSWLLVKYSQAMIRPLSAVGIIFGCAAMFGYLGAMEWIYRPVTNGPATNPLT
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
AMCMIFIGTALWISKKKSDANLALLLLSAVIIITLTLIFDVISGSNFSAILTPFQEQVAL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHH
ELKNGKSNSMGINSSVMFLFIAISLVLFVGQKLIASQLFAFIALAIPTVSFTGYAYGLEQ
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYGQMSMLTAFIGFVLAIAALMMTAKVGGVNAILSPFIGGKIARRQTAAGYFVPAILGYL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHH
LIKSLCAIDGELCGIFVVTICWFVILMISYSALFQEKMDLERRQMEQLLTLAAMNDQLTG
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
LANRRKFHEFAQEELNRVQRNKSQFWVLVMDVDFFKKINDTAGHDMGDQVLIELSKVLKN
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
SVRAVDLVSRMGGEEFSIILSDTTKQGAERVAESIRSSIESIKIKGWTDIYGPITASIGG
HHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHCCCC
ATSDGADKLENTLKIADSALYQAKQRGRNQVYFAAE
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MLESKVSENSWLLVKYSQAMIRPLSAVGIIFGCAAMFGYLGAMEWIYRPVTNGPATNPLT
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
AMCMIFIGTALWISKKKSDANLALLLLSAVIIITLTLIFDVISGSNFSAILTPFQEQVAL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHH
ELKNGKSNSMGINSSVMFLFIAISLVLFVGQKLIASQLFAFIALAIPTVSFTGYAYGLEQ
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYGQMSMLTAFIGFVLAIAALMMTAKVGGVNAILSPFIGGKIARRQTAAGYFVPAILGYL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHH
LIKSLCAIDGELCGIFVVTICWFVILMISYSALFQEKMDLERRQMEQLLTLAAMNDQLTG
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
LANRRKFHEFAQEELNRVQRNKSQFWVLVMDVDFFKKINDTAGHDMGDQVLIELSKVLKN
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
SVRAVDLVSRMGGEEFSIILSDTTKQGAERVAESIRSSIESIKIKGWTDIYGPITASIGG
HHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHCCCC
ATSDGADKLENTLKIADSALYQAKQRGRNQVYFAAE
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]