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The map label for this gene is trpS [H]

Identifier: 157960075

GI number: 157960075

Start: 275753

End: 276763

Strand: Direct

Name: trpS [H]

Synonym: Spea_0246

Alternate gene names: 157960075

Gene position: 275753-276763 (Clockwise)

Preceding gene: 157960074

Following gene: 157960077

Centisome position: 5.33

GC content: 46.88

Gene sequence:

>1011_bases
ATGACGACCCCCCTAACGAAACCAGTAGTTCTAAGCGGAGCGCAGCCATCGGGCGAGCTAACCATCGGCAACTATATGGG
CGCGTTACGTCAGTGGGTTGCAATGCAAGATAGTCATGATTGCCTTTATTGCGTCGTTGACCTGCATGCGATTACTGTCA
GACAAGACCCAGTAAAATTACGTGAGGCGTGTCTTGATACGTTAGCACTGTATTTAGCTTGTGGTGTTGACCCTAAAAAG
AGCACGGTATTTATCCAATCTCAGGTGCCACAGCACACTCAGCTTGGCTGGGCGCTAAACTGTTACACCCAGATGGGTGA
ACTAAACCGTATGACGCAGTTTAAAGACAAGTCTCAGAAGCATGCTAACAACATCAACGTCGGCCTATTTGGTTACCCTG
TATTGATGGCTGCTGATATCTTGCTTTACCAAGCGAACGAAATCCCTGTGGGTCAAGATCAGAAGCAACACTTAGAGCTG
ACTCGTGATATCGCGACACGCTTTAACAATGCTTATGGCGAAACATTTGTCGTTCCAGAACCATTTATTCCTGAGCATGG
CGCTAAGGTGATGTCACTACAAGATCCGCTGAAGAAGATGTCTAAGTCTGACGATAACCGTAATAACGTAATCGGCCTGC
TTGAAGATCCGAAAGCTATCATGAAGAAGATCAAAAAAGCGATGACAGATGGCCAAGAGCCGCCAGTTGTACGTTTCGAC
GTTGCTGAAAAGCCAGGTGTATCAAACCTACTGAGTCTAATGTCTGGCTGTACTGGTCAGTCAATTGCCAGTCTTGAAGC
TGAGTTTGAAGGCAAGATGTATGGTCATTTGAAAGTGGCAACCGGTGAAGCCGTTGTCGGTATGCTTGAGCCGCTACAGC
AGCGCTTTAAAGAATACCGTGCAGACCAAGACTTCTTGAATCAAGTGATGAGAGACGGAGCTGAAAAAGCACAGGCTCGC
GCTGAAGTCACAATCAAGAACGTCTACGAGAAGATCGGTCTTATCGTTTAA

Upstream 100 bases:

>100_bases
AAGATATCGGGCTAAGCGGCCCAGATGCCGTATGTGAACAATTTAGTGAAATAACCGCGCTACTTAATCAGTGCGATAAA
GCAACGGAGCAATAAACAAA

Downstream 100 bases:

>100_bases
CTCGATGCGAGTTAATGAAAGCCAGCGATATTCGCTGGCTTTTTTGTTGGGTTATTCATAGATTAGCTACTGTAGGCTTA
ACCAAGTCACAAAGGCGGCA

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTTPLTKPVVLSGAQPSGELTIGNYMGALRQWVAMQDSHDCLYCVVDLHAITVRQDPVKLREACLDTLALYLACGVDPKK
STVFIQSQVPQHTQLGWALNCYTQMGELNRMTQFKDKSQKHANNINVGLFGYPVLMAADILLYQANEIPVGQDQKQHLEL
TRDIATRFNNAYGETFVVPEPFIPEHGAKVMSLQDPLKKMSKSDDNRNNVIGLLEDPKAIMKKIKKAMTDGQEPPVVRFD
VAEKPGVSNLLSLMSGCTGQSIASLEAEFEGKMYGHLKVATGEAVVGMLEPLQQRFKEYRADQDFLNQVMRDGAEKAQAR
AEVTIKNVYEKIGLIV

Sequences:

>Translated_336_residues
MTTPLTKPVVLSGAQPSGELTIGNYMGALRQWVAMQDSHDCLYCVVDLHAITVRQDPVKLREACLDTLALYLACGVDPKK
STVFIQSQVPQHTQLGWALNCYTQMGELNRMTQFKDKSQKHANNINVGLFGYPVLMAADILLYQANEIPVGQDQKQHLEL
TRDIATRFNNAYGETFVVPEPFIPEHGAKVMSLQDPLKKMSKSDDNRNNVIGLLEDPKAIMKKIKKAMTDGQEPPVVRFD
VAEKPGVSNLLSLMSGCTGQSIASLEAEFEGKMYGHLKVATGEAVVGMLEPLQQRFKEYRADQDFLNQVMRDGAEKAQAR
AEVTIKNVYEKIGLIV
>Mature_335_residues
TTPLTKPVVLSGAQPSGELTIGNYMGALRQWVAMQDSHDCLYCVVDLHAITVRQDPVKLREACLDTLALYLACGVDPKKS
TVFIQSQVPQHTQLGWALNCYTQMGELNRMTQFKDKSQKHANNINVGLFGYPVLMAADILLYQANEIPVGQDQKQHLELT
RDIATRFNNAYGETFVVPEPFIPEHGAKVMSLQDPLKKMSKSDDNRNNVIGLLEDPKAIMKKIKKAMTDGQEPPVVRFDV
AEKPGVSNLLSLMSGCTGQSIASLEAEFEGKMYGHLKVATGEAVVGMLEPLQQRFKEYRADQDFLNQVMRDGAEKAQARA
EVTIKNVYEKIGLIV

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=331, Percent_Identity=41.6918429003021, Blast_Score=274, Evalue=9e-74,
Organism=Homo sapiens, GI41352700, Length=179, Percent_Identity=51.9553072625698, Blast_Score=201, Evalue=9e-52,
Organism=Escherichia coli, GI1789786, Length=331, Percent_Identity=71.9033232628399, Blast_Score=516, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI71982800, Length=342, Percent_Identity=34.2105263157895, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI71982793, Length=342, Percent_Identity=34.2105263157895, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6320474, Length=345, Percent_Identity=40.2898550724638, Blast_Score=238, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24666151, Length=327, Percent_Identity=40.6727828746177, Blast_Score=251, Evalue=4e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 37316; Mature: 37184

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPLTKPVVLSGAQPSGELTIGNYMGALRQWVAMQDSHDCLYCVVDLHAITVRQDPVKL
CCCCCCCCEEEECCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEEHHHEEECCCHHHH
REACLDTLALYLACGVDPKKSTVFIQSQVPQHTQLGWALNCYTQMGELNRMTQFKDKSQK
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHH
HANNINVGLFGYPVLMAADILLYQANEIPVGQDQKQHLELTRDIATRFNNAYGETFVVPE
HCCCCCEEHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCC
PFIPEHGAKVMSLQDPLKKMSKSDDNRNNVIGLLEDPKAIMKKIKKAMTDGQEPPVVRFD
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEEE
VAEKPGVSNLLSLMSGCTGQSIASLEAEFEGKMYGHLKVATGEAVVGMLEPLQQRFKEYR
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHC
ADQDFLNQVMRDGAEKAQARAEVTIKNVYEKIGLIV
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTPLTKPVVLSGAQPSGELTIGNYMGALRQWVAMQDSHDCLYCVVDLHAITVRQDPVKL
CCCCCCCEEEECCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEEHHHEEECCCHHHH
REACLDTLALYLACGVDPKKSTVFIQSQVPQHTQLGWALNCYTQMGELNRMTQFKDKSQK
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHH
HANNINVGLFGYPVLMAADILLYQANEIPVGQDQKQHLELTRDIATRFNNAYGETFVVPE
HCCCCCEEHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCC
PFIPEHGAKVMSLQDPLKKMSKSDDNRNNVIGLLEDPKAIMKKIKKAMTDGQEPPVVRFD
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEEE
VAEKPGVSNLLSLMSGCTGQSIASLEAEFEGKMYGHLKVATGEAVVGMLEPLQQRFKEYR
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHC
ADQDFLNQVMRDGAEKAQARAEVTIKNVYEKIGLIV
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]