Click here to switch to the map view.

The map label for this gene is gph [C]

Identifier: 157960074

GI number: 157960074

Start: 275049

End: 275747

Strand: Direct

Name: gph [C]

Synonym: Spea_0245

Alternate gene names: 157960074

Gene position: 275049-275747 (Clockwise)

Preceding gene: 157960073

Following gene: 157960075

Centisome position: 5.32

GC content: 47.21

Gene sequence:

>699_bases
ATGAACCCTTTTAGTCAAATCAACGCGGTAGCATTTGACTTAGACGGAACGCTGATCGATAGCGTTCCGGATCTAGCGGC
TGCCACGCGCGCGACCCTAGAAGAGCTAAACCTTCCACTTTGCAGCGATGAACTAGTGCGAAGCTGGGTGGGTAATGGCG
CTGAGATGCTAATGCGTAGAGCATTATCTTTTGCCCTTGCTACTGAAGTGAGTGAAGAAAAGCTGCAAAACACGATGCCG
CGCTTTATGCATTTTTATAAGCAAAACCTGCAGCAACACAGCGTGCTATATGCCGGTGTGGAGTCGGTATTATCCCAGCT
CAAGCAAGCGGGCTATCGGATGGCGATTGTCACCAACAAGCCTTACGAATTCACCATACCGCTACTTAAAGCATTTAATA
TTCATGGTTACTTTGACATAGTGCTTGGCGGAGACTCATTAACAAAGATGAAACCCGATCCATTGCCACTGCAGCATATT
TTGCACGAGTGGCAGTTAGAATCTGAGCAGTTGTTGATGGTTGGCGACTCAAAGAATGACATATTAGCGGCAAAAGCGGC
TAAGGTTGCTTCTGTGGGCTTGACCTATGGCTACAACTACGGTGAAGATATCGGGCTAAGCGGCCCAGATGCCGTATGTG
AACAATTTAGTGAAATAACCGCGCTACTTAATCAGTGCGATAAAGCAACGGAGCAATAA

Upstream 100 bases:

>100_bases
GGACATGTTTGTTGCAGGTTCAGCTATTTTCAATAAGCCTGATTACAAGGCGGTAATCGACGAGATGCGTGCAGAACTTG
CAAGCGTTGAGGCGTAATCA

Downstream 100 bases:

>100_bases
ACAAAATGACGACCCCCCTAACGAAACCAGTAGTTCTAAGCGGAGCGCAGCCATCGGGCGAGCTAACCATCGGCAACTAT
ATGGGCGCGTTACGTCAGTG

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MNPFSQINAVAFDLDGTLIDSVPDLAAATRATLEELNLPLCSDELVRSWVGNGAEMLMRRALSFALATEVSEEKLQNTMP
RFMHFYKQNLQQHSVLYAGVESVLSQLKQAGYRMAIVTNKPYEFTIPLLKAFNIHGYFDIVLGGDSLTKMKPDPLPLQHI
LHEWQLESEQLLMVGDSKNDILAAKAAKVASVGLTYGYNYGEDIGLSGPDAVCEQFSEITALLNQCDKATEQ

Sequences:

>Translated_232_residues
MNPFSQINAVAFDLDGTLIDSVPDLAAATRATLEELNLPLCSDELVRSWVGNGAEMLMRRALSFALATEVSEEKLQNTMP
RFMHFYKQNLQQHSVLYAGVESVLSQLKQAGYRMAIVTNKPYEFTIPLLKAFNIHGYFDIVLGGDSLTKMKPDPLPLQHI
LHEWQLESEQLLMVGDSKNDILAAKAAKVASVGLTYGYNYGEDIGLSGPDAVCEQFSEITALLNQCDKATEQ
>Mature_232_residues
MNPFSQINAVAFDLDGTLIDSVPDLAAATRATLEELNLPLCSDELVRSWVGNGAEMLMRRALSFALATEVSEEKLQNTMP
RFMHFYKQNLQQHSVLYAGVESVLSQLKQAGYRMAIVTNKPYEFTIPLLKAFNIHGYFDIVLGGDSLTKMKPDPLPLQHI
LHEWQLESEQLLMVGDSKNDILAAKAAKVASVGLTYGYNYGEDIGLSGPDAVCEQFSEITALLNQCDKATEQ

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=240, Percent_Identity=41.6666666666667, Blast_Score=189, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 25675; Mature: 25675

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPFSQINAVAFDLDGTLIDSVPDLAAATRATLEELNLPLCSDELVRSWVGNGAEMLMRR
CCCHHHHCEEEEECCCHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHH
ALSFALATEVSEEKLQNTMPRFMHFYKQNLQQHSVLYAGVESVLSQLKQAGYRMAIVTNK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
PYEFTIPLLKAFNIHGYFDIVLGGDSLTKMKPDPLPLQHILHEWQLESEQLLMVGDSKND
CCEEEHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCC
ILAAKAAKVASVGLTYGYNYGEDIGLSGPDAVCEQFSEITALLNQCDKATEQ
CHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNPFSQINAVAFDLDGTLIDSVPDLAAATRATLEELNLPLCSDELVRSWVGNGAEMLMRR
CCCHHHHCEEEEECCCHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHH
ALSFALATEVSEEKLQNTMPRFMHFYKQNLQQHSVLYAGVESVLSQLKQAGYRMAIVTNK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
PYEFTIPLLKAFNIHGYFDIVLGGDSLTKMKPDPLPLQHILHEWQLESEQLLMVGDSKND
CCEEEHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCC
ILAAKAAKVASVGLTYGYNYGEDIGLSGPDAVCEQFSEITALLNQCDKATEQ
CHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA