Click here to switch to the map view.

The map label for this gene is argH [H]

Identifier: 157960060

GI number: 157960060

Start: 259924

End: 261294

Strand: Direct

Name: argH [H]

Synonym: Spea_0231

Alternate gene names: 157960060

Gene position: 259924-261294 (Clockwise)

Preceding gene: 157960059

Following gene: 157960062

Centisome position: 5.02

GC content: 46.17

Gene sequence:

>1371_bases
ATGGCATTATGGGGCGGCAGATTTAGCCAAGAGAGCAGTGCACTGTTTAAACTTTTCAATGACTCATTACCTGTGGACTT
TCGACTCATAGAGCAAGACATTGTCGGCTCAATTGCATGGGCAAGTGCCATTACCCAGGTTGGGATCCTTACCGAGAAAG
AATGCACCGAGCTACACCAAGCGTTAAATGAACTACTCGCGGAAACGCAAGATAGTCCTGAACTTATCATTGCTTCAGGA
GCTGAGGATATTCATAGTTTTGTTGAGCAGTCGCTGATTGCTAAAGTGGGAGACCTAGGTAAAAAGCTACATACGGGCCG
CTCGCGTAACGATCAGGTAGCGACGGATCTTAAGCTTTGGTGTAAGAAAGAAGGTGAGCAGTTACTCGGTTTACTTGCTA
ACTTAAGAGCGGCATTGATCGGACTTGCCGAGCGTGAAATCGATGCGGTGATGCCGGGTTATACACATCTGCAAAGAGCA
CAGCCAATATTGTTTGGTCATTGGTGTTTAGCTTATGTGGAGATGTTTGAACGTGATATTAGCCGTCTGCAAGATGCGCT
TAAGCGTGCCGATACTTGTCCGTTAGGTACGGGAGCATTAGCAGGTACAGCTTATCCGATGGACAGGGTGAAGCTGGCAA
AATCCTTAGGGTTTGCCTCGCCGACACTCAATAGTTTAGATACGGTTTCAGACCGAGATCATGTGATTGAGATCTGCAGC
AATGCCTCTATCAGTATGATGCACTTGAGTCGGATGGCTGAAGATTTAATCTTCTTTAATAGTGGTGAAGCGGGCTTTAT
TGAATTAGACGATGAAGTCACGTCAGGCTCGTCACTGATGCCGCAGAAGAAAAACCCTGATGCATTGGAGCTAATTAGAG
GCAAGACGGGTCGTGTTTACGGCGCCTTAATGGGAATATTAACCACCATGAAAGCATTGCCGCTTGCGTACAACAAAGAT
ATGCAGGAAGACAAAGAAGGCCTGTTCGATGTAATGGATAGCTGGTCGATCTGTTTAGAAATGGCAGCCTTGGTATTAAG
TGGCTTGCAGGTTAATAGAGAAAAAACCCTTAAGGCTGCTAAGCAAGGTTATGCTAACTCGACTGAACTGGCTGATTATT
TAGTGGCGAAAGGGATGCCTTTTAGAGAAGCACACCACGTGGTTGGTGAAGCAGTCGTGAGTGCTATTGCCAAGCAAACC
CCTCTAGAAGAGTTAACTCTAGTAGAGCTGCAAGCCTTTAGCGCAGCGATTGAAGCCGACGTATATGATTGCTTGACCAT
TGAGTCTTGCTTAGCTAAGCGTGAAGCGCTAGGTGGTACCTCTTTATCTCAAGTTCGTAACGCATTGGCGACTAAGAAAG
AGAACTGCTAA

Upstream 100 bases:

>100_bases
AATAAAAGTTAGTTAGCGCTAACGTGTATGAGTTAGCGCTCTATAAACATAGAGTTAAAGGTGAAGCTAGACTTTGCCTA
GATATAAAGAGGAAGTCAGA

Downstream 100 bases:

>100_bases
TAGTGCAATTAGTAGGTGTAAAAAAACGCGGTCATTCTGTTGAGTGACCGCGTTTTTATTATTTCTGACTATGGTTTACT
GGAATAGGTTTACGAGAAAA

Product: argininosuccinate lyase

Products: NA

Alternate protein names: ASAL; Arginosuccinase [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MALWGGRFSQESSALFKLFNDSLPVDFRLIEQDIVGSIAWASAITQVGILTEKECTELHQALNELLAETQDSPELIIASG
AEDIHSFVEQSLIAKVGDLGKKLHTGRSRNDQVATDLKLWCKKEGEQLLGLLANLRAALIGLAEREIDAVMPGYTHLQRA
QPILFGHWCLAYVEMFERDISRLQDALKRADTCPLGTGALAGTAYPMDRVKLAKSLGFASPTLNSLDTVSDRDHVIEICS
NASISMMHLSRMAEDLIFFNSGEAGFIELDDEVTSGSSLMPQKKNPDALELIRGKTGRVYGALMGILTTMKALPLAYNKD
MQEDKEGLFDVMDSWSICLEMAALVLSGLQVNREKTLKAAKQGYANSTELADYLVAKGMPFREAHHVVGEAVVSAIAKQT
PLEELTLVELQAFSAAIEADVYDCLTIESCLAKREALGGTSLSQVRNALATKKENC

Sequences:

>Translated_456_residues
MALWGGRFSQESSALFKLFNDSLPVDFRLIEQDIVGSIAWASAITQVGILTEKECTELHQALNELLAETQDSPELIIASG
AEDIHSFVEQSLIAKVGDLGKKLHTGRSRNDQVATDLKLWCKKEGEQLLGLLANLRAALIGLAEREIDAVMPGYTHLQRA
QPILFGHWCLAYVEMFERDISRLQDALKRADTCPLGTGALAGTAYPMDRVKLAKSLGFASPTLNSLDTVSDRDHVIEICS
NASISMMHLSRMAEDLIFFNSGEAGFIELDDEVTSGSSLMPQKKNPDALELIRGKTGRVYGALMGILTTMKALPLAYNKD
MQEDKEGLFDVMDSWSICLEMAALVLSGLQVNREKTLKAAKQGYANSTELADYLVAKGMPFREAHHVVGEAVVSAIAKQT
PLEELTLVELQAFSAAIEADVYDCLTIESCLAKREALGGTSLSQVRNALATKKENC
>Mature_455_residues
ALWGGRFSQESSALFKLFNDSLPVDFRLIEQDIVGSIAWASAITQVGILTEKECTELHQALNELLAETQDSPELIIASGA
EDIHSFVEQSLIAKVGDLGKKLHTGRSRNDQVATDLKLWCKKEGEQLLGLLANLRAALIGLAEREIDAVMPGYTHLQRAQ
PILFGHWCLAYVEMFERDISRLQDALKRADTCPLGTGALAGTAYPMDRVKLAKSLGFASPTLNSLDTVSDRDHVIEICSN
ASISMMHLSRMAEDLIFFNSGEAGFIELDDEVTSGSSLMPQKKNPDALELIRGKTGRVYGALMGILTTMKALPLAYNKDM
QEDKEGLFDVMDSWSICLEMAALVLSGLQVNREKTLKAAKQGYANSTELADYLVAKGMPFREAHHVVGEAVVSAIAKQTP
LEELTLVELQAFSAAIEADVYDCLTIESCLAKREALGGTSLSQVRNALATKKENC

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI31541964, Length=443, Percent_Identity=45.372460496614, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI68303542, Length=443, Percent_Identity=45.372460496614, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI68303549, Length=443, Percent_Identity=43.3408577878104, Blast_Score=335, Evalue=5e-92,
Organism=Homo sapiens, GI68303547, Length=443, Percent_Identity=42.8893905191874, Blast_Score=331, Evalue=9e-91,
Organism=Escherichia coli, GI1790398, Length=450, Percent_Identity=67.7777777777778, Blast_Score=623, Evalue=1e-180,
Organism=Saccharomyces cerevisiae, GI6321806, Length=454, Percent_Identity=44.9339207048458, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI221473854, Length=443, Percent_Identity=41.9864559819413, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI78706858, Length=443, Percent_Identity=41.9864559819413, Blast_Score=343, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF00206 Lyase_1 [H]

EC number: =4.3.2.1 [H]

Molecular weight: Translated: 49788; Mature: 49656

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALWGGRFSQESSALFKLFNDSLPVDFRLIEQDIVGSIAWASAITQVGILTEKECTELHQ
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ALNELLAETQDSPELIIASGAEDIHSFVEQSLIAKVGDLGKKLHTGRSRNDQVATDLKLW
HHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
CKKEGEQLLGLLANLRAALIGLAEREIDAVMPGYTHLQRAQPILFGHWCLAYVEMFERDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
SRLQDALKRADTCPLGTGALAGTAYPMDRVKLAKSLGFASPTLNSLDTVSDRDHVIEICS
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHC
NASISMMHLSRMAEDLIFFNSGEAGFIELDDEVTSGSSLMPQKKNPDALELIRGKTGRVY
CCCHHHHHHHHHHHHHEEECCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCHHH
GALMGILTTMKALPLAYNKDMQEDKEGLFDVMDSWSICLEMAALVLSGLQVNREKTLKAA
HHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KQGYANSTELADYLVAKGMPFREAHHVVGEAVVSAIAKQTPLEELTLVELQAFSAAIEAD
HHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VYDCLTIESCLAKREALGGTSLSQVRNALATKKENC
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALWGGRFSQESSALFKLFNDSLPVDFRLIEQDIVGSIAWASAITQVGILTEKECTELHQ
CCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ALNELLAETQDSPELIIASGAEDIHSFVEQSLIAKVGDLGKKLHTGRSRNDQVATDLKLW
HHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
CKKEGEQLLGLLANLRAALIGLAEREIDAVMPGYTHLQRAQPILFGHWCLAYVEMFERDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
SRLQDALKRADTCPLGTGALAGTAYPMDRVKLAKSLGFASPTLNSLDTVSDRDHVIEICS
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHC
NASISMMHLSRMAEDLIFFNSGEAGFIELDDEVTSGSSLMPQKKNPDALELIRGKTGRVY
CCCHHHHHHHHHHHHHEEECCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCHHH
GALMGILTTMKALPLAYNKDMQEDKEGLFDVMDSWSICLEMAALVLSGLQVNREKTLKAA
HHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KQGYANSTELADYLVAKGMPFREAHHVVGEAVVSAIAKQTPLEELTLVELQAFSAAIEAD
HHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VYDCLTIESCLAKREALGGTSLSQVRNALATKKENC
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA