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The map label for this gene is trmA [H]

Identifier: 157959994

GI number: 157959994

Start: 179017

End: 180114

Strand: Reverse

Name: trmA [H]

Synonym: Spea_0164

Alternate gene names: 157959994

Gene position: 180114-179017 (Counterclockwise)

Preceding gene: 157959996

Following gene: 157959992

Centisome position: 3.48

GC content: 41.53

Gene sequence:

>1098_bases
ATGAATTTAGCAGCAATGGATCCTACAACCTATGATGTGCAACTAGAAGCAAAGTGCGTCAAGTTAAAGCAGTTATTTGC
TGATTTTGATACCCCTGAACTGGAAACATTCAGTTCCGAACCTGCTCACTACCGTATGCGTGCTGAGTTTAGAGTTTGGC
ATGAAGGTGAAGACCTCTATTACTATATGTTTGATAAAGAGCTTAATAGTAAAGTTCGTTGTGATCAGTTCCTGCCGGCA
AGCGAATTGATTAATAAAATGATGCCTGCTTTAGTAGAGTTATTAAAGCCGAATACTATTTTACGCCACCGTCTGTTCCA
AATTGATTTCTTGTCCACGCTAAGCGGCGAGATTTTAGTTTCACTGTTATATCACAAGCAGCTGGATAGTGAGTGGGAAC
AACAGGCTAAAATCCTTAAACAAACTCTTAGCACCCAGTTTAACGTAAACTTAATTGGCCGAGCGCGTAAGCAGAAGATC
ATCTTTGATAAAGACTTCGTGGTTGAGTCACTCAATGTTGCTGGCAAACAACTACAGTATCATCAGATTGAGAACAGCTT
CACTCAGCCAAACGGTAAGGTGTCAGTTAAGATGCTTGAGTGGGCTATTGATATCACCAAGAATAGCAGTGGTGATCTAC
TAGAGCTTTATTGTGGTAACGGTAACTTCTCTATCGCGTTAGCACAAAACTTTGAGCGCGTATTAGCAACTGAACTGGCC
AAGCCTTCTGTTGAGTCAGCGCAATACAATATCAAAGTTAACCAAGTCGAGAACCTGCAGATCATCCGTATGTCAGCAGA
AGACTTTACCGAAGCTATGGCGAAGAAGCGCAGCTTTAGACGCTTAGAAGGTATCGATTTAGATAGTTATAACTGTAATA
CCATTTTTGTTGACCCACCACGAGCTGGGCTAGATGCGGATACGGTTAAGCTTGTGCAAGGTTATGAGCGTATCGTTTAT
ATCTCATGTAACCCTAATACCCTTATCGATAACTTAGCTGAGCTGAGCAAGACCCATAAGATCACTCGCTTTGCCTTATT
CGATCAGTTCCCTTATACGGATCATATGGAGTCAGGGGTTTTCCTTGAGAAAAAATAA

Upstream 100 bases:

>100_bases
TTTTCCCACAACCTAGGAAAAATCGGAAGTGAGAAACTGGACATCCCAGCTATCTAGTATAATATTCCGCCCCTAATTTA
ATGCCAACGGTGATCAAGAT

Downstream 100 bases:

>100_bases
CTTGAAGTAAATATTCAAAAGTCTCATTAAAAACGGCATCTTTATGGATGCCGTTTTTGTTTCAGTTAATTTTGTTCTTT
CTTGTTTTGTAGTGCATCAG

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MNLAAMDPTTYDVQLEAKCVKLKQLFADFDTPELETFSSEPAHYRMRAEFRVWHEGEDLYYYMFDKELNSKVRCDQFLPA
SELINKMMPALVELLKPNTILRHRLFQIDFLSTLSGEILVSLLYHKQLDSEWEQQAKILKQTLSTQFNVNLIGRARKQKI
IFDKDFVVESLNVAGKQLQYHQIENSFTQPNGKVSVKMLEWAIDITKNSSGDLLELYCGNGNFSIALAQNFERVLATELA
KPSVESAQYNIKVNQVENLQIIRMSAEDFTEAMAKKRSFRRLEGIDLDSYNCNTIFVDPPRAGLDADTVKLVQGYERIVY
ISCNPNTLIDNLAELSKTHKITRFALFDQFPYTDHMESGVFLEKK

Sequences:

>Translated_365_residues
MNLAAMDPTTYDVQLEAKCVKLKQLFADFDTPELETFSSEPAHYRMRAEFRVWHEGEDLYYYMFDKELNSKVRCDQFLPA
SELINKMMPALVELLKPNTILRHRLFQIDFLSTLSGEILVSLLYHKQLDSEWEQQAKILKQTLSTQFNVNLIGRARKQKI
IFDKDFVVESLNVAGKQLQYHQIENSFTQPNGKVSVKMLEWAIDITKNSSGDLLELYCGNGNFSIALAQNFERVLATELA
KPSVESAQYNIKVNQVENLQIIRMSAEDFTEAMAKKRSFRRLEGIDLDSYNCNTIFVDPPRAGLDADTVKLVQGYERIVY
ISCNPNTLIDNLAELSKTHKITRFALFDQFPYTDHMESGVFLEKK
>Mature_365_residues
MNLAAMDPTTYDVQLEAKCVKLKQLFADFDTPELETFSSEPAHYRMRAEFRVWHEGEDLYYYMFDKELNSKVRCDQFLPA
SELINKMMPALVELLKPNTILRHRLFQIDFLSTLSGEILVSLLYHKQLDSEWEQQAKILKQTLSTQFNVNLIGRARKQKI
IFDKDFVVESLNVAGKQLQYHQIENSFTQPNGKVSVKMLEWAIDITKNSSGDLLELYCGNGNFSIALAQNFERVLATELA
KPSVESAQYNIKVNQVENLQIIRMSAEDFTEAMAKKRSFRRLEGIDLDSYNCNTIFVDPPRAGLDADTVKLVQGYERIVY
ISCNPNTLIDNLAELSKTHKITRFALFDQFPYTDHMESGVFLEKK

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=164, Percent_Identity=26.8292682926829, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI51173878, Length=164, Percent_Identity=26.8292682926829, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1790403, Length=356, Percent_Identity=60.3932584269663, Blast_Score=469, Evalue=1e-134,
Organism=Escherichia coli, GI1789148, Length=185, Percent_Identity=32.972972972973, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71997438, Length=163, Percent_Identity=28.2208588957055, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24666579, Length=203, Percent_Identity=28.5714285714286, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011869
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.35 [H]

Molecular weight: Translated: 42104; Mature: 42104

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLAAMDPTTYDVQLEAKCVKLKQLFADFDTPELETFSSEPAHYRMRAEFRVWHEGEDLY
CCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCHHCCCCCCCEEEEEEEEEEECCCCEE
YYMFDKELNSKVRCDQFLPASELINKMMPALVELLKPNTILRHRLFQIDFLSTLSGEILV
EEEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHH
SLLYHKQLDSEWEQQAKILKQTLSTQFNVNLIGRARKQKIIFDKDFVVESLNVAGKQLQY
HHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHHHHH
HQIENSFTQPNGKVSVKMLEWAIDITKNSSGDLLELYCGNGNFSIALAQNFERVLATELA
HHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHHC
KPSVESAQYNIKVNQVENLQIIRMSAEDFTEAMAKKRSFRRLEGIDLDSYNCNTIFVDPP
CCCCCCEEEEEEEEEECCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCC
RAGLDADTVKLVQGYERIVYISCNPNTLIDNLAELSKTHKITRFALFDQFPYTDHMESGV
CCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCE
FLEKK
EEECC
>Mature Secondary Structure
MNLAAMDPTTYDVQLEAKCVKLKQLFADFDTPELETFSSEPAHYRMRAEFRVWHEGEDLY
CCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCHHCCCCCCCEEEEEEEEEEECCCCEE
YYMFDKELNSKVRCDQFLPASELINKMMPALVELLKPNTILRHRLFQIDFLSTLSGEILV
EEEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHH
SLLYHKQLDSEWEQQAKILKQTLSTQFNVNLIGRARKQKIIFDKDFVVESLNVAGKQLQY
HHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHHHHH
HQIENSFTQPNGKVSVKMLEWAIDITKNSSGDLLELYCGNGNFSIALAQNFERVLATELA
HHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHHC
KPSVESAQYNIKVNQVENLQIIRMSAEDFTEAMAKKRSFRRLEGIDLDSYNCNTIFVDPP
CCCCCCEEEEEEEEEECCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCC
RAGLDADTVKLVQGYERIVYISCNPNTLIDNLAELSKTHKITRFALFDQFPYTDHMESGV
CCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCE
FLEKK
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA