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The map label for this gene is epsD [H]

Identifier: 157959974

GI number: 157959974

Start: 159791

End: 161923

Strand: Direct

Name: epsD [H]

Synonym: Spea_0144

Alternate gene names: 157959974

Gene position: 159791-161923 (Clockwise)

Preceding gene: 157959973

Following gene: 157959975

Centisome position: 3.09

GC content: 46.04

Gene sequence:

>2133_bases
ATGAATAATAAGCGGATTAGACGCAGATTAATTGCCGGTATGGTGATGGGGGCTTCCTTACTGGCCCCACAACTTGCATG
GTCTGAACAATATGCGGCTAACTTTAAAGGCACAGATATCCAAGAGTTTATCAATATTGTTGGTAAAAACTTGAATCGAA
CTATTATTGTCGACCCAACCGTACGCGGAAAAATCAATGTTCGTAGTTACGATCTACTAAACGACGAACAGTATTACCAG
TTCTTCCTCAACGTATTACAAGTCTATGGTTATGCTGTTGTCGAGATGGACAACAACATCATAAAGGTAATCAAAGATAA
AGATGCTAAAACGGCATCAATCCGTGTCGCCGATGATGATGCACCAGGCTTGGGTGACGAGATGGTTACCCGTATCGTCG
CACTGTATAACACAGAAGCAAAGCAGCTAGCCCCTCTATTACGTCAGCTAAACGATAACGCCGGCGGCGGTAACGTAGTT
AACTACGATCCGTCCAATGTTTTGATGATCTCTGGTCGTGCGGCCGTGGTTAATAAACTGGTAGAGATCGTGCGCCGCGT
AGATAAGCAAGGTGATACAGCCGTTGAAGTGGTTAAGCTTGAATATGCCTCAGCCGGTGAAATCGTTCGTATTATAGATA
CCTTGTACCGTTCAACGGCTAATCAAGCACAGATGCCTGGCCAAGCACCAAAAGTTGTTGCCGATGAGCGTACCAACGCT
GTGGTGGTAAGTGGTGATGAGAAGAGTCGTGAGCGAGTGGTTAAGCTGATTAAAAACCTAGATGCAGAGCAAGCAAGTAC
GGGTAACACTAAGGTCCGCTACCTGCGTTATGCAAAAGCCGAAGATTTAGTCGAGGTGTTAACGGGTTTTGCTGAACAAC
TTGTCGATGATAAAGGCACCTCACAAGGTGGCGGCAGCAAGCGTCGAAATGAAATTAATATCATGGCCCACAATGACACC
AATGCCTTGGTGATTAGTGCGGAACCAGATCAGATGCGCACCATCGAGAGCGTGATTAATCAGCTGGATATTCGCCGAGC
ACAAGTACTGGTTGAAGCGATCATTGTAGAAGTTGGCGAAGGTGATGATGTTGGTTTTGGTGTTCAGTGGGCAACCGAAG
CTGGCGGTGGTACTCAGTTTAATAACCTAGGCCCAACCATTGGTGAAATTGGTGCAGGTGTTTGGCAGGCTCAAGGTGAA
GAGGGCTCAACAGTCTGTAACGACGGTACTTGTACCGAGAATCCTGACACTCGTGGTGATATTACCTTGCTTGCTCAAGC
GCTTGGTAAGGTCAATGGTATGGCGTGGGGCGTGGCAATGGGCGATTTTGCTGCCTTGATTCAAGCCGTGTCGAGTGATA
CCAAATCAAATGTTCTTGCTACACCATCTATCACTACGCTTGATAACCAAGAAGCCTCATTTATCGTAGGTGATGAAGTC
CCTATTTTAACCGGTAGTCAAAATTCTAGTAACGGTAACAGCAATCCATTCCAAACGGTTGAGCGTAAGGAAGTGGGCGT
AAAGCTTAAAGTTGTTCCTCAAATTAACGAAGGAAGCTCGGTTAAGCTGACAATCGAGCAGGAAGTTTCAGGTATTAACG
GTAAGACTGGCGTCGATGTAACCTTTGCTACTCGTCGTTTAACCACAACGGTAATGGCTGATTCAGGCCAAATCGTGGTA
CTCGGTGGTTTGATTAAAGAAGAGGTGCAAGAGTCAGTACAAAAAGTCCCGTTCTTGGGTGATATCCCGATCATTGGCCA
CCTATTTAAGTCCTCTTCTAGCGGTAAGAAAAAAACCAACTTGATGGTATTTATCAAGCCAACGATTATCCGCGACGGTA
TGACCATGGAAGGCATCGCAGGACGTAAGTATAATTACTTCCGAGCGCTGCAACTCGAGCAGCAGGAACGCGGCGTTAAC
TTAATGCCAAATACCGATGTGCCGATCCTCGAAGAGTGGAATCAAGCTGATTATCTGCCTCCTGAAGTCAACGACGTGCT
GAACCGTTACAAAGAAGGTAAAGGTTTAGACACAAAGATGCGTGAAACCGATCCGGCGCTAAAGTCTATCTCGAATAGCA
AAGATGCCGATAAGCAAGGCACTGATACAGATAAAGAAACTTCTGATGAGTGA

Upstream 100 bases:

>100_bases
TAGTCTGCCGCAATAGAATAAGCAGCAGAAAATAACAATAAAATTTGCAGTAATACACATTGAGTTTGCCGCAACGAAAG
GGTTTAGGGGAAGTATAACA

Downstream 100 bases:

>100_bases
GAATTCAATTATAGTCGATGAAGAGCTAGTGCAGGTCTCGAATGAGCTTGGACTAGTGTCTGAGGAAGAGGCGGATCAGC
TTTTTCATTCGAATAGCAAA

Product: general secretion pathway protein D

Products: NA

Alternate protein names: Cholera toxin secretion protein epsD [H]

Number of amino acids: Translated: 710; Mature: 710

Protein sequence:

>710_residues
MNNKRIRRRLIAGMVMGASLLAPQLAWSEQYAANFKGTDIQEFINIVGKNLNRTIIVDPTVRGKINVRSYDLLNDEQYYQ
FFLNVLQVYGYAVVEMDNNIIKVIKDKDAKTASIRVADDDAPGLGDEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVV
NYDPSNVLMISGRAAVVNKLVEIVRRVDKQGDTAVEVVKLEYASAGEIVRIIDTLYRSTANQAQMPGQAPKVVADERTNA
VVVSGDEKSRERVVKLIKNLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAEQLVDDKGTSQGGGSKRRNEINIMAHNDT
NALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVGEGDDVGFGVQWATEAGGGTQFNNLGPTIGEIGAGVWQAQGE
EGSTVCNDGTCTENPDTRGDITLLAQALGKVNGMAWGVAMGDFAALIQAVSSDTKSNVLATPSITTLDNQEASFIVGDEV
PILTGSQNSSNGNSNPFQTVERKEVGVKLKVVPQINEGSSVKLTIEQEVSGINGKTGVDVTFATRRLTTTVMADSGQIVV
LGGLIKEEVQESVQKVPFLGDIPIIGHLFKSSSSGKKKTNLMVFIKPTIIRDGMTMEGIAGRKYNYFRALQLEQQERGVN
LMPNTDVPILEEWNQADYLPPEVNDVLNRYKEGKGLDTKMRETDPALKSISNSKDADKQGTDTDKETSDE

Sequences:

>Translated_710_residues
MNNKRIRRRLIAGMVMGASLLAPQLAWSEQYAANFKGTDIQEFINIVGKNLNRTIIVDPTVRGKINVRSYDLLNDEQYYQ
FFLNVLQVYGYAVVEMDNNIIKVIKDKDAKTASIRVADDDAPGLGDEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVV
NYDPSNVLMISGRAAVVNKLVEIVRRVDKQGDTAVEVVKLEYASAGEIVRIIDTLYRSTANQAQMPGQAPKVVADERTNA
VVVSGDEKSRERVVKLIKNLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAEQLVDDKGTSQGGGSKRRNEINIMAHNDT
NALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVGEGDDVGFGVQWATEAGGGTQFNNLGPTIGEIGAGVWQAQGE
EGSTVCNDGTCTENPDTRGDITLLAQALGKVNGMAWGVAMGDFAALIQAVSSDTKSNVLATPSITTLDNQEASFIVGDEV
PILTGSQNSSNGNSNPFQTVERKEVGVKLKVVPQINEGSSVKLTIEQEVSGINGKTGVDVTFATRRLTTTVMADSGQIVV
LGGLIKEEVQESVQKVPFLGDIPIIGHLFKSSSSGKKKTNLMVFIKPTIIRDGMTMEGIAGRKYNYFRALQLEQQERGVN
LMPNTDVPILEEWNQADYLPPEVNDVLNRYKEGKGLDTKMRETDPALKSISNSKDADKQGTDTDKETSDE
>Mature_710_residues
MNNKRIRRRLIAGMVMGASLLAPQLAWSEQYAANFKGTDIQEFINIVGKNLNRTIIVDPTVRGKINVRSYDLLNDEQYYQ
FFLNVLQVYGYAVVEMDNNIIKVIKDKDAKTASIRVADDDAPGLGDEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVV
NYDPSNVLMISGRAAVVNKLVEIVRRVDKQGDTAVEVVKLEYASAGEIVRIIDTLYRSTANQAQMPGQAPKVVADERTNA
VVVSGDEKSRERVVKLIKNLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAEQLVDDKGTSQGGGSKRRNEINIMAHNDT
NALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVGEGDDVGFGVQWATEAGGGTQFNNLGPTIGEIGAGVWQAQGE
EGSTVCNDGTCTENPDTRGDITLLAQALGKVNGMAWGVAMGDFAALIQAVSSDTKSNVLATPSITTLDNQEASFIVGDEV
PILTGSQNSSNGNSNPFQTVERKEVGVKLKVVPQINEGSSVKLTIEQEVSGINGKTGVDVTFATRRLTTTVMADSGQIVV
LGGLIKEEVQESVQKVPFLGDIPIIGHLFKSSSSGKKKTNLMVFIKPTIIRDGMTMEGIAGRKYNYFRALQLEQQERGVN
LMPNTDVPILEEWNQADYLPPEVNDVLNRYKEGKGLDTKMRETDPALKSISNSKDADKQGTDTDKETSDE

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=653, Percent_Identity=45.635528330781, Blast_Score=550, Evalue=1e-157,
Organism=Escherichia coli, GI1789793, Length=344, Percent_Identity=24.4186046511628, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845
- InterPro:   IPR001814 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 77265; Mature: 77265

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKRIRRRLIAGMVMGASLLAPQLAWSEQYAANFKGTDIQEFINIVGKNLNRTIIVDPT
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHCCCCCCEEEECCC
VRGKINVRSYDLLNDEQYYQFFLNVLQVYGYAVVEMDNNIIKVIKDKDAKTASIRVADDD
CCCEEEEEEEECCCCHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCCEEEEEEECCC
APGLGDEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVVNYDPSNVLMISGRAAVVNKL
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCHHHHHHH
VEIVRRVDKQGDTAVEVVKLEYASAGEIVRIIDTLYRSTANQAQMPGQAPKVVADERTNA
HHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCHHCCCCCCCCEEECCCCCE
VVVSGDEKSRERVVKLIKNLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAEQLVDDKGT
EEEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCC
SQGGGSKRRNEINIMAHNDTNALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVGE
CCCCCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GDDVGFGVQWATEAGGGTQFNNLGPTIGEIGAGVWQAQGEEGSTVCNDGTCTENPDTRGD
CCCCCCCEEEECCCCCCCCCCCCCCCHHHHCCCCEECCCCCCCEEECCCCCCCCCCCCCH
ITLLAQALGKVNGMAWGVAMGDFAALIQAVSSDTKSNVLATPSITTLDNQEASFIVGDEV
HHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCCCEEECCCEEECCCCCCEEEECCCC
PILTGSQNSSNGNSNPFQTVERKEVGVKLKVVPQINEGSSVKLTIEQEVSGINGKTGVDV
EEEECCCCCCCCCCCCHHHHHHHHCCEEEEEEEECCCCCEEEEEEHHHCCCCCCCCCCEE
TFATRRLTTTVMADSGQIVVLGGLIKEEVQESVQKVPFLGDIPIIGHLFKSSSSGKKKTN
EEEHHEEEEEEEECCCCEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCC
LMVFIKPTIIRDGMTMEGIAGRKYNYFRALQLEQQERGVNLMPNTDVPILEEWNQADYLP
EEEEEEEEEEECCCCCCCCCCCCCCEEEEEEEHHHHCCCCCCCCCCCCHHHCCCCCCCCC
PEVNDVLNRYKEGKGLDTKMRETDPALKSISNSKDADKQGTDTDKETSDE
CHHHHHHHHHHCCCCCCCHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNNKRIRRRLIAGMVMGASLLAPQLAWSEQYAANFKGTDIQEFINIVGKNLNRTIIVDPT
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHCCCCCCEEEECCC
VRGKINVRSYDLLNDEQYYQFFLNVLQVYGYAVVEMDNNIIKVIKDKDAKTASIRVADDD
CCCEEEEEEEECCCCHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCCEEEEEEECCC
APGLGDEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVVNYDPSNVLMISGRAAVVNKL
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCHHHHHHH
VEIVRRVDKQGDTAVEVVKLEYASAGEIVRIIDTLYRSTANQAQMPGQAPKVVADERTNA
HHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCHHCCCCCCCCEEECCCCCE
VVVSGDEKSRERVVKLIKNLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAEQLVDDKGT
EEEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCC
SQGGGSKRRNEINIMAHNDTNALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVGE
CCCCCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GDDVGFGVQWATEAGGGTQFNNLGPTIGEIGAGVWQAQGEEGSTVCNDGTCTENPDTRGD
CCCCCCCEEEECCCCCCCCCCCCCCCHHHHCCCCEECCCCCCCEEECCCCCCCCCCCCCH
ITLLAQALGKVNGMAWGVAMGDFAALIQAVSSDTKSNVLATPSITTLDNQEASFIVGDEV
HHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCCCEEECCCEEECCCCCCEEEECCCC
PILTGSQNSSNGNSNPFQTVERKEVGVKLKVVPQINEGSSVKLTIEQEVSGINGKTGVDV
EEEECCCCCCCCCCCCHHHHHHHHCCEEEEEEEECCCCCEEEEEEHHHCCCCCCCCCCEE
TFATRRLTTTVMADSGQIVVLGGLIKEEVQESVQKVPFLGDIPIIGHLFKSSSSGKKKTN
EEEHHEEEEEEEECCCCEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCC
LMVFIKPTIIRDGMTMEGIAGRKYNYFRALQLEQQERGVNLMPNTDVPILEEWNQADYLP
EEEEEEEEEEECCCCCCCCCCCCCCEEEEEEEHHHHCCCCCCCCCCCCHHHCCCCCCCCC
PEVNDVLNRYKEGKGLDTKMRETDPALKSISNSKDADKQGTDTDKETSDE
CHHHHHHHHHHCCCCCCCHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]