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The map label for this gene is lepB [H]

Identifier: 157959964

GI number: 157959964

Start: 149524

End: 150183

Strand: Reverse

Name: lepB [H]

Synonym: Spea_0134

Alternate gene names: 157959964

Gene position: 150183-149524 (Counterclockwise)

Preceding gene: 157959965

Following gene: 157959961

Centisome position: 2.9

GC content: 44.85

Gene sequence:

>660_bases
ATGAATAAAGTGAAACAGCTTTGGAAAGAAAATCGTCAGCTTGTGCTATTCATTGTTTTAATGTCTGTTTGCAGAAGTGC
TATCGCCGACTGGTACACAGTACCGACAGGCTCTATGCAGCCTACGATTAAAGAGGGCGACCGCATTGTGGTCGATAAAC
TGGCCTACGACTTACATGTGCCCTTTACTCAAATATCATTAGCCACAACTGGCGAGCCTGAACGCGGTGAAATAGTGGTG
TTTGAATCAAAAGCCGCTGATAAACGTTTAATCAAGCGGGTTATTGGCTTACCTGGCGATACCATTAGCTTAAGCAATGA
AGTGCTATTTATTAATGGCAAGGCGTTGGCTTACTCGGTGGTATCTAGCAGTGCTCAAGAATTAATTGCTAAGGAAGATC
TCAATGGCTTATCCCATAGCATCCGTATTGAAAAGCAGGCCAGTGATCAGTTAAGTAGCTTCGAAACGGTAATTGTGCCC
GAAGGGCATTATATGGTGATGGGCGATAACCGCCGTAACAGCGCTGACTCACGGGTATATGGCTTTGTCCCTCGGGATGA
AATCAAAGGTAAAGCAACCCAAGTTGCTTTCTCACTGGATTATGAAGACAACTACATACCCCGCCAAGATCGGTTTTTCA
CTAGCCTTTATGGCGGGTAA

Upstream 100 bases:

>100_bases
TAAGGGAACAACCATTATGTCGTCCATTCGCCTTGAATTAGTTTGCCAAAAAGCACTCTGAGTAGATCAACTTCTTATAC
TGATTGGTATAAGGTGCTTT

Downstream 100 bases:

>100_bases
ACACTGTTAACTCAAGTCAGTACATGGCGTAACCTCTGTAAGTGGTTACGCCATCAATACTTAACCTAACACTGCAAGTG
CTTAATCTCGCGCTGCGATC

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MNKVKQLWKENRQLVLFIVLMSVCRSAIADWYTVPTGSMQPTIKEGDRIVVDKLAYDLHVPFTQISLATTGEPERGEIVV
FESKAADKRLIKRVIGLPGDTISLSNEVLFINGKALAYSVVSSSAQELIAKEDLNGLSHSIRIEKQASDQLSSFETVIVP
EGHYMVMGDNRRNSADSRVYGFVPRDEIKGKATQVAFSLDYEDNYIPRQDRFFTSLYGG

Sequences:

>Translated_219_residues
MNKVKQLWKENRQLVLFIVLMSVCRSAIADWYTVPTGSMQPTIKEGDRIVVDKLAYDLHVPFTQISLATTGEPERGEIVV
FESKAADKRLIKRVIGLPGDTISLSNEVLFINGKALAYSVVSSSAQELIAKEDLNGLSHSIRIEKQASDQLSSFETVIVP
EGHYMVMGDNRRNSADSRVYGFVPRDEIKGKATQVAFSLDYEDNYIPRQDRFFTSLYGG
>Mature_219_residues
MNKVKQLWKENRQLVLFIVLMSVCRSAIADWYTVPTGSMQPTIKEGDRIVVDKLAYDLHVPFTQISLATTGEPERGEIVV
FESKAADKRLIKRVIGLPGDTISLSNEVLFINGKALAYSVVSSSAQELIAKEDLNGLSHSIRIEKQASDQLSSFETVIVP
EGHYMVMGDNRRNSADSRVYGFVPRDEIKGKATQVAFSLDYEDNYIPRQDRFFTSLYGG

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=246, Percent_Identity=34.9593495934959, Blast_Score=114, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019756
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF00717 Peptidase_S24 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 24526; Mature: 24526

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVKQLWKENRQLVLFIVLMSVCRSAIADWYTVPTGSMQPTIKEGDRIVVDKLAYDLHV
CCHHHHHHHCCCCEEHHHHHHHHHHHHHHCEEECCCCCCCCCHHCCCEEEEEEHHHEEEC
PFTQISLATTGEPERGEIVVFESKAADKRLIKRVIGLPGDTISLSNEVLFINGKALAYSV
CCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEECCCEEEEECCHHHHHH
VSSSAQELIAKEDLNGLSHSIRIEKQASDQLSSFETVIVPEGHYMVMGDNRRNSADSRVY
HHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCEEEEECCCEEEEECCCCCCCCCEEE
GFVPRDEIKGKATQVAFSLDYEDNYIPRQDRFFTSLYGG
ECCCHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MNKVKQLWKENRQLVLFIVLMSVCRSAIADWYTVPTGSMQPTIKEGDRIVVDKLAYDLHV
CCHHHHHHHCCCCEEHHHHHHHHHHHHHHCEEECCCCCCCCCHHCCCEEEEEEHHHEEEC
PFTQISLATTGEPERGEIVVFESKAADKRLIKRVIGLPGDTISLSNEVLFINGKALAYSV
CCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEECCCEEEEECCHHHHHH
VSSSAQELIAKEDLNGLSHSIRIEKQASDQLSSFETVIVPEGHYMVMGDNRRNSADSRVY
HHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCEEEEECCCEEEEECCCCCCCCCEEE
GFVPRDEIKGKATQVAFSLDYEDNYIPRQDRFFTSLYGG
ECCCHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1546969 [H]