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The map label for this gene is moeA [H]

Identifier: 157959958

GI number: 157959958

Start: 139967

End: 141211

Strand: Reverse

Name: moeA [H]

Synonym: Spea_0128

Alternate gene names: 157959958

Gene position: 141211-139967 (Counterclockwise)

Preceding gene: 157959960

Following gene: 157959957

Centisome position: 2.73

GC content: 49.88

Gene sequence:

>1245_bases
ATGCTGACTCATGCAGATCCATGTTCTCAACCTAGCCTAATGCATCCCGATCAGGCTATCGTTAAATTACTAGAACAAGT
TAATCCAATCACAGACTCTGAAGTCGTGACACTGCCACAAGCGTTGGGTCGAGTACTTGCTGAAGATCTCGCCTCTCATA
TCGACCTCCCACCATTCGATAATTCGGCAATGGATGGCTATGCCTTCAATTTTGCCGACTTAAGTGCTAACGCGCCGCTA
AAATTAATCGGCCAATCTTTCGCAGGTCACCCATTTACAGGTGAATGTATCGAAGGGGGTTGCATTCGCATCATGACCGG
TGCCCCAGTGCCTAGCGGTTACGATACGGTACAGATGCAGGAAAAGGTCACAGCCGATGGCGAGCAGATCACTATTGAAG
CGCCTAAAGAGCTTGGCGCTAACGTGCGCCTTCGCGGTGAAGAGCTCACAAGTGGCACTAAGGTATTAACTGCGGGCACA
TTGATTGGCGCGGCAGAAATGGGCGTACTGGCAACAATAGGCGCAAGCCAGTTACGCGTTAAACGTATCGTTAAAGTCGC
TTTCTTCTCTACCGGTGACGAGCTACGCCCAGTTGGAAGCGAACTAGGCCCAGGACAAATATACGACTCTAACCGCTATA
GTATTCAAGGCTTACTGAGCCGCGGCAATATCGAATGGATCGATCTTGGCGTCATCGAAGATGATAAAGAAGCGATTCGC
CAAGCTTTTAAAACCGCATCAGAATGCGCCGATATGGTACTGACCTCGGGCGGAGTCTCCGTAGGCGACGCCGACTATAC
CAAGCAGATTTTAGATGAAGAGGGTCAGATCACCTTCTGGAAGCTAGCCATTAAACCGGGTAAACCTTTTGCCTTTGGTC
ACCTTGGAGATGCCGTCTTCTGTGGGCTACCTGGCAACCCAGTTTCATCCATGGTGACTTTCTACAAGCTTGTGTGGCCA
CTACTCAATAAGATGCAGGGCTTACCTCAAGTTACACCGGTAACACTGCAAGCTAGGCTCAAAGGTGAGATTCGTAAGGC
CCCTGGTCGCGTGGAATACCAACGCGCCATCTTAAGCTATAACGCGAAAGGTGAAGCCGAAGTTGAAGTCACCGGTGGCC
AAGGTTCAGGAATGCTGACCTCCATGACTCTGGCGAACTGCTTCATTATCTTGGCTCAGGATTGCGATGGTTTAAGCAAT
GATACCCAGGTGACGGTTGAGCCTTTTAATAGCGTGCTTTGCTAA

Upstream 100 bases:

>100_bases
AAAACTGCCGCGCAGATCATAGCCATCTACATTCACCGTGGCATAATGCACTATATTCGTTTTTACTTAACGTTCCGATT
TAACTCTCAAGGATAGATAG

Downstream 100 bases:

>100_bases
AACGAGCACCGCGTAAACATATGAGATCAATTTGATGTCGACCGAACAAATAGCAACAGATGAAATTTTATCTGATGCGG
AAATATTACGTTATAGCCGC

Product: molybdenum cofactor synthesis domain-containing protein

Products: NA

Alternate protein names: MPT Mo-transferase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MLTHADPCSQPSLMHPDQAIVKLLEQVNPITDSEVVTLPQALGRVLAEDLASHIDLPPFDNSAMDGYAFNFADLSANAPL
KLIGQSFAGHPFTGECIEGGCIRIMTGAPVPSGYDTVQMQEKVTADGEQITIEAPKELGANVRLRGEELTSGTKVLTAGT
LIGAAEMGVLATIGASQLRVKRIVKVAFFSTGDELRPVGSELGPGQIYDSNRYSIQGLLSRGNIEWIDLGVIEDDKEAIR
QAFKTASECADMVLTSGGVSVGDADYTKQILDEEGQITFWKLAIKPGKPFAFGHLGDAVFCGLPGNPVSSMVTFYKLVWP
LLNKMQGLPQVTPVTLQARLKGEIRKAPGRVEYQRAILSYNAKGEAEVEVTGGQGSGMLTSMTLANCFIILAQDCDGLSN
DTQVTVEPFNSVLC

Sequences:

>Translated_414_residues
MLTHADPCSQPSLMHPDQAIVKLLEQVNPITDSEVVTLPQALGRVLAEDLASHIDLPPFDNSAMDGYAFNFADLSANAPL
KLIGQSFAGHPFTGECIEGGCIRIMTGAPVPSGYDTVQMQEKVTADGEQITIEAPKELGANVRLRGEELTSGTKVLTAGT
LIGAAEMGVLATIGASQLRVKRIVKVAFFSTGDELRPVGSELGPGQIYDSNRYSIQGLLSRGNIEWIDLGVIEDDKEAIR
QAFKTASECADMVLTSGGVSVGDADYTKQILDEEGQITFWKLAIKPGKPFAFGHLGDAVFCGLPGNPVSSMVTFYKLVWP
LLNKMQGLPQVTPVTLQARLKGEIRKAPGRVEYQRAILSYNAKGEAEVEVTGGQGSGMLTSMTLANCFIILAQDCDGLSN
DTQVTVEPFNSVLC
>Mature_414_residues
MLTHADPCSQPSLMHPDQAIVKLLEQVNPITDSEVVTLPQALGRVLAEDLASHIDLPPFDNSAMDGYAFNFADLSANAPL
KLIGQSFAGHPFTGECIEGGCIRIMTGAPVPSGYDTVQMQEKVTADGEQITIEAPKELGANVRLRGEELTSGTKVLTAGT
LIGAAEMGVLATIGASQLRVKRIVKVAFFSTGDELRPVGSELGPGQIYDSNRYSIQGLLSRGNIEWIDLGVIEDDKEAIR
QAFKTASECADMVLTSGGVSVGDADYTKQILDEEGQITFWKLAIKPGKPFAFGHLGDAVFCGLPGNPVSSMVTFYKLVWP
LLNKMQGLPQVTPVTLQARLKGEIRKAPGRVEYQRAILSYNAKGEAEVEVTGGQGSGMLTSMTLANCFIILAQDCDGLSN
DTQVTVEPFNSVLC

Specific function: Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP [H]

COG id: COG0303

COG function: function code H; Molybdopterin biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moeA family [H]

Homologues:

Organism=Homo sapiens, GI66932975, Length=390, Percent_Identity=35.8974358974359, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI10880983, Length=390, Percent_Identity=35.8974358974359, Blast_Score=219, Evalue=3e-57,
Organism=Escherichia coli, GI1787049, Length=402, Percent_Identity=49.7512437810945, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI25147659, Length=333, Percent_Identity=32.4324324324324, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17562606, Length=316, Percent_Identity=28.4810126582279, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI17136978, Length=361, Percent_Identity=31.0249307479224, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24638841, Length=361, Percent_Identity=31.0249307479224, Blast_Score=138, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020817
- InterPro:   IPR008284
- InterPro:   IPR005111
- InterPro:   IPR005110
- InterPro:   IPR001453 [H]

Pfam domain/function: PF00994 MoCF_biosynth; PF03454 MoeA_C; PF03453 MoeA_N [H]

EC number: NA

Molecular weight: Translated: 44252; Mature: 44252

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS01079 MOCF_BIOSYNTHESIS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTHADPCSQPSLMHPDQAIVKLLEQVNPITDSEVVTLPQALGRVLAEDLASHIDLPPFD
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCC
NSAMDGYAFNFADLSANAPLKLIGQSFAGHPFTGECIEGGCIRIMTGAPVPSGYDTVQMQ
CCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCHHCCCCEEEEEECCCCCCCCCHHHHH
EKVTADGEQITIEAPKELGANVRLRGEELTSGTKVLTAGTLIGAAEMGVLATIGASQLRV
HHHCCCCCEEEEECHHHHCCCEEEECCCCCCCCEEEEECHHHHHHHHCHHHHCCHHHHHH
KRIVKVAFFSTGDELRPVGSELGPGQIYDSNRYSIQGLLSRGNIEWIDLGVIEDDKEAIR
HHHHHHHEECCCCCCCCCCCCCCCCCEECCCCEEEEEECCCCCEEEEEEEEEECHHHHHH
QAFKTASECADMVLTSGGVSVGDADYTKQILDEEGQITFWKLAIKPGKPFAFGHLGDAVF
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCEEEECCCCEEE
CGLPGNPVSSMVTFYKLVWPLLNKMQGLPQVTPVTLQARLKGEIRKAPGRVEYQRAILSY
ECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHEE
NAKGEAEVEVTGGQGSGMLTSMTLANCFIILAQDCDGLSNDTQVTVEPFNSVLC
CCCCCEEEEEECCCCCCHHHHHHHHHEEEEEEECCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MLTHADPCSQPSLMHPDQAIVKLLEQVNPITDSEVVTLPQALGRVLAEDLASHIDLPPFD
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCC
NSAMDGYAFNFADLSANAPLKLIGQSFAGHPFTGECIEGGCIRIMTGAPVPSGYDTVQMQ
CCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCHHCCCCEEEEEECCCCCCCCCHHHHH
EKVTADGEQITIEAPKELGANVRLRGEELTSGTKVLTAGTLIGAAEMGVLATIGASQLRV
HHHCCCCCEEEEECHHHHCCCEEEECCCCCCCCEEEEECHHHHHHHHCHHHHCCHHHHHH
KRIVKVAFFSTGDELRPVGSELGPGQIYDSNRYSIQGLLSRGNIEWIDLGVIEDDKEAIR
HHHHHHHEECCCCCCCCCCCCCCCCCEECCCCEEEEEECCCCCEEEEEEEEEECHHHHHH
QAFKTASECADMVLTSGGVSVGDADYTKQILDEEGQITFWKLAIKPGKPFAFGHLGDAVF
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCEEEECCCCEEE
CGLPGNPVSSMVTFYKLVWPLLNKMQGLPQVTPVTLQARLKGEIRKAPGRVEYQRAILSY
ECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHEE
NAKGEAEVEVTGGQGSGMLTSMTLANCFIILAQDCDGLSNDTQVTVEPFNSVLC
CCCCCEEEEEECCCCCCHHHHHHHHHEEEEEEECCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3045084; 8905232; 9278503; 9515915 [H]