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The map label for this gene is moeB [H]

Identifier: 157959957

GI number: 157959957

Start: 139162

End: 139932

Strand: Reverse

Name: moeB [H]

Synonym: Spea_0127

Alternate gene names: 157959957

Gene position: 139932-139162 (Counterclockwise)

Preceding gene: 157959958

Following gene: 157959954

Centisome position: 2.7

GC content: 46.43

Gene sequence:

>771_bases
ATGTCGACCGAACAAATAGCAACAGATGAAATTTTATCTGATGCGGAAATATTACGTTATAGCCGCCAAATATCGATTAA
ATCAATGGACTTTGAAGGCCAGGAAAAACTCAAGCAAGCTAAGGTGCTCATTATCGGAGCTGGCGGTTTAGGTTGCGCCG
CGAGTCAATATCTTGCCGTAGCAGGTGCTGGCTCAATGACCTTAGTCGATTTTGATACCGTCGAGATATCAAATCTGCAA
AGACAGGTCTTACATCAAGACGCCAACGTCGGCCAAGCTAAAGTCGACTCGGCTAAGCAGAGTTTAACGGCACTTAACCC
GCACATTCAGATAGAAACAATCAATGCGGTCCTCGATGATCATGAGATCGATGCCCTCGTAGAGCAACACTCTATCGTGA
TGGATTGCACCGACAATGTTGCCGTGCGCGAACAGCTAAACCAAAGCTGCTTTAAGCACAAGAAGCCACTTATCTCGGCT
GCTGCGATTCGTATGGAAGGCATGGTCACGGTATTCGACTACCAAGACGATACTCCTTGCTACCACTGCTTTAGTAAGCT
TTTTGGTGAGCAACAACTTAGCTGTGTTGAATCTGGGATCTTAGCCCCCGTTGTAGGCATGATTGGTTGCTTACAAGCGG
TTGAAGCCATCAAGGTGATTGCTAATATGGGACAAACTCTAGCTGGGCGCATCCTGATGATCGATGCTATGACCATGGAG
TTTAGAGAGATGAAACTCCCAAAACTGCCGCAGTGTAAGGTTTGTAGCTAA

Upstream 100 bases:

>100_bases
GATTGCGATGGTTTAAGCAATGATACCCAGGTGACGGTTGAGCCTTTTAATAGCGTGCTTTGCTAAAACGAGCACCGCGT
AAACATATGAGATCAATTTG

Downstream 100 bases:

>100_bases
CCCATTTTGCTCCCATATTGACCTTTGTCATTAGCATATTAACTGACAAACAAGTAAACAAACGCAGCTCTTGGGCTGCG
TTTGTTGTTTAAAAGCAAAT

Product: molybdopterin biosynthesis protein MoeB

Products: NA

Alternate protein names: MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSTEQIATDEILSDAEILRYSRQISIKSMDFEGQEKLKQAKVLIIGAGGLGCAASQYLAVAGAGSMTLVDFDTVEISNLQ
RQVLHQDANVGQAKVDSAKQSLTALNPHIQIETINAVLDDHEIDALVEQHSIVMDCTDNVAVREQLNQSCFKHKKPLISA
AAIRMEGMVTVFDYQDDTPCYHCFSKLFGEQQLSCVESGILAPVVGMIGCLQAVEAIKVIANMGQTLAGRILMIDAMTME
FREMKLPKLPQCKVCS

Sequences:

>Translated_256_residues
MSTEQIATDEILSDAEILRYSRQISIKSMDFEGQEKLKQAKVLIIGAGGLGCAASQYLAVAGAGSMTLVDFDTVEISNLQ
RQVLHQDANVGQAKVDSAKQSLTALNPHIQIETINAVLDDHEIDALVEQHSIVMDCTDNVAVREQLNQSCFKHKKPLISA
AAIRMEGMVTVFDYQDDTPCYHCFSKLFGEQQLSCVESGILAPVVGMIGCLQAVEAIKVIANMGQTLAGRILMIDAMTME
FREMKLPKLPQCKVCS
>Mature_255_residues
STEQIATDEILSDAEILRYSRQISIKSMDFEGQEKLKQAKVLIIGAGGLGCAASQYLAVAGAGSMTLVDFDTVEISNLQR
QVLHQDANVGQAKVDSAKQSLTALNPHIQIETINAVLDDHEIDALVEQHSIVMDCTDNVAVREQLNQSCFKHKKPLISAA
AIRMEGMVTVFDYQDDTPCYHCFSKLFGEQQLSCVESGILAPVVGMIGCLQAVEAIKVIANMGQTLAGRILMIDAMTMEF
REMKLPKLPQCKVCS

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein moaD [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=238, Percent_Identity=44.9579831932773, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI4885649, Length=154, Percent_Identity=33.1168831168831, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI23510338, Length=175, Percent_Identity=31.4285714285714, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI23510340, Length=175, Percent_Identity=31.4285714285714, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI38045948, Length=163, Percent_Identity=32.5153374233129, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI38045944, Length=124, Percent_Identity=33.0645161290323, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI38045942, Length=124, Percent_Identity=33.0645161290323, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1787048, Length=245, Percent_Identity=62.4489795918367, Blast_Score=340, Evalue=8e-95,
Organism=Escherichia coli, GI87082356, Length=245, Percent_Identity=37.9591836734694, Blast_Score=167, Evalue=8e-43,
Organism=Escherichia coli, GI1789177, Length=153, Percent_Identity=28.1045751633987, Blast_Score=68, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17540406, Length=248, Percent_Identity=37.9032258064516, Blast_Score=191, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI193203301, Length=156, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI86565433, Length=162, Percent_Identity=28.3950617283951, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI86565431, Length=162, Percent_Identity=28.3950617283951, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71984476, Length=116, Percent_Identity=31.8965517241379, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6321903, Length=220, Percent_Identity=38.6363636363636, Blast_Score=162, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6322639, Length=165, Percent_Identity=32.1212121212121, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320598, Length=156, Percent_Identity=26.2820512820513, Blast_Score=65, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6325438, Length=102, Percent_Identity=34.3137254901961, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582879, Length=259, Percent_Identity=42.0849420849421, Blast_Score=219, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24660640, Length=155, Percent_Identity=31.6129032258064, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI20129973, Length=109, Percent_Identity=33.0275229357798, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI28573937, Length=161, Percent_Identity=27.9503105590062, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24641311, Length=255, Percent_Identity=25.4901960784314, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR012730
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 27953; Mature: 27822

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEQIATDEILSDAEILRYSRQISIKSMDFEGQEKLKQAKVLIIGAGGLGCAASQYLAV
CCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHCEEEEEECCCCCHHHHHHHEE
AGAGSMTLVDFDTVEISNLQRQVLHQDANVGQAKVDSAKQSLTALNPHIQIETINAVLDD
ECCCCEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHCC
HEIDALVEQHSIVMDCTDNVAVREQLNQSCFKHKKPLISAAAIRMEGMVTVFDYQDDTPC
HHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCCCCHH
YHCFSKLFGEQQLSCVESGILAPVVGMIGCLQAVEAIKVIANMGQTLAGRILMIDAMTME
HHHHHHHHCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEEEHHHHH
FREMKLPKLPQCKVCS
HHHCCCCCCCCCCCCC
>Mature Secondary Structure 
STEQIATDEILSDAEILRYSRQISIKSMDFEGQEKLKQAKVLIIGAGGLGCAASQYLAV
CCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHCEEEEEECCCCCHHHHHHHEE
AGAGSMTLVDFDTVEISNLQRQVLHQDANVGQAKVDSAKQSLTALNPHIQIETINAVLDD
ECCCCEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHCC
HEIDALVEQHSIVMDCTDNVAVREQLNQSCFKHKKPLISAAAIRMEGMVTVFDYQDDTPC
HHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCCCCHH
YHCFSKLFGEQQLSCVESGILAPVVGMIGCLQAVEAIKVIANMGQTLAGRILMIDAMTME
HHHHHHHHCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEEEHHHHH
FREMKLPKLPQCKVCS
HHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3045084; 8905232; 9278503 [H]