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The map label for this gene is rfaB [H]

Identifier: 157959945

GI number: 157959945

Start: 128921

End: 129997

Strand: Reverse

Name: rfaB [H]

Synonym: Spea_0115

Alternate gene names: 157959945

Gene position: 129997-128921 (Counterclockwise)

Preceding gene: 157959954

Following gene: 157959940

Centisome position: 2.51

GC content: 39.74

Gene sequence:

>1077_bases
ATGAAAAAGATTGCTATCTTTTGCCACAACTTTTATGCAAACGGAACAGTCAAAGTTGCGTTAACTCAAGCAGAGATTTT
GCAAGAAGCTGGACAAGATGTACATCTATTCGTTTTCAAGCAAGAGGGAGATTTTCCTCCGCCACAAAACGTAACTATTC
ACTATGTTTATAAAAACCAGAAAGAGCTAAAACTTAAAGAGCAACAGCAACAATTAAAACGGTGCGTTGAGGCTGTCGAG
GACTCAAGCGGGAAGTTTGATCTATTTTTATCCAACTCGACAGACTGCGATACAGTTGTTAGTGGCTGTAACTTTGATCC
CTGTTATTACTTTTGCCACTGTGCGTTAAAACAAGAATTAATCATGGAGCTCAAGCGAGGTCCAATCCACTTCTATCGAC
GCTGGATAAAAGCCAAAGCTTTAATTAAAAAAAATATCATCACGGTTTCTGAAGGCATTGCGGATGAGCTAAAAAATACG
GGCTGGCTAAAAGCTAAAAGTATTCAAGCTATTTACAACCCTTTTAGAATTGAAGATATAAGACATAAAGCATTGGCTGA
AAACGATGAAATCCCAAATGAGCCCTTTATGATCCATATTGGACGAATTACGCGACAGAAACGTTTAGACATCCTTTTTC
AAGCGCTTGCCCTAATGCCATCGGCTCCTAAATTAGTATTACTAACCAATAGACCCGAAAGAGCACGAAAGCTAGCGAAA
AAATATAATGTCGAAAAACGCATTATTACACCCGGCTTTCAATCGAATCCTTACGCTTGGATTGCACGTGCAGAATTGAT
GCTACTTAGTTCAGACTTTGAGGGTTTTGGACTCGTAATTGCAGAGTCTCTTATCTGCGGAACCCCTGTGGTAAGTACAG
ATTGCCCTTACGGTCCAAATGAAATTCTAACTGGAGAGCTAGCAAGTTACTTAGTTCCTCGCAGAAATCCAAAAGCGTTA
GCTGAATGTGCAGTAGCATGCCTCGCAGCCCCTCCTCAATTAGAGAATATCGATATTATAAAAAAGGTAGATAGTCGTCA
TATTGCACAAAAATACATTGAGCTCTGTCATCAGTAA

Upstream 100 bases:

>100_bases
TCGAGACTCTCTTCCTCAAAGTCAATGCATTTACTTATGCTCACTAGCAGCACATCTGATATAATTCTTACCTTAATATT
ATGTAAGAGACATATGTAGA

Downstream 100 bases:

>100_bases
AATTGCGCTATATTCTCCAATAGCCTAATTTGCGTCGCATCTTTAAGTGGCGCAATAAATTTAATGGCTTACGCTTAAGT
CCGGCAGTACCATTTTCAAG

Product: group 1 glycosyl transferase

Products: NA

Alternate protein names: UDP-D-galactose--(Glucosyl)lipopolysaccharide-alpha-1,3-D-galactosyltransferase [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKKIAIFCHNFYANGTVKVALTQAEILQEAGQDVHLFVFKQEGDFPPPQNVTIHYVYKNQKELKLKEQQQQLKRCVEAVE
DSSGKFDLFLSNSTDCDTVVSGCNFDPCYYFCHCALKQELIMELKRGPIHFYRRWIKAKALIKKNIITVSEGIADELKNT
GWLKAKSIQAIYNPFRIEDIRHKALAENDEIPNEPFMIHIGRITRQKRLDILFQALALMPSAPKLVLLTNRPERARKLAK
KYNVEKRIITPGFQSNPYAWIARAELMLLSSDFEGFGLVIAESLICGTPVVSTDCPYGPNEILTGELASYLVPRRNPKAL
AECAVACLAAPPQLENIDIIKKVDSRHIAQKYIELCHQ

Sequences:

>Translated_358_residues
MKKIAIFCHNFYANGTVKVALTQAEILQEAGQDVHLFVFKQEGDFPPPQNVTIHYVYKNQKELKLKEQQQQLKRCVEAVE
DSSGKFDLFLSNSTDCDTVVSGCNFDPCYYFCHCALKQELIMELKRGPIHFYRRWIKAKALIKKNIITVSEGIADELKNT
GWLKAKSIQAIYNPFRIEDIRHKALAENDEIPNEPFMIHIGRITRQKRLDILFQALALMPSAPKLVLLTNRPERARKLAK
KYNVEKRIITPGFQSNPYAWIARAELMLLSSDFEGFGLVIAESLICGTPVVSTDCPYGPNEILTGELASYLVPRRNPKAL
AECAVACLAAPPQLENIDIIKKVDSRHIAQKYIELCHQ
>Mature_358_residues
MKKIAIFCHNFYANGTVKVALTQAEILQEAGQDVHLFVFKQEGDFPPPQNVTIHYVYKNQKELKLKEQQQQLKRCVEAVE
DSSGKFDLFLSNSTDCDTVVSGCNFDPCYYFCHCALKQELIMELKRGPIHFYRRWIKAKALIKKNIITVSEGIADELKNT
GWLKAKSIQAIYNPFRIEDIRHKALAENDEIPNEPFMIHIGRITRQKRLDILFQALALMPSAPKLVLLTNRPERARKLAK
KYNVEKRIITPGFQSNPYAWIARAELMLLSSDFEGFGLVIAESLICGTPVVSTDCPYGPNEILTGELASYLVPRRNPKAL
AECAVACLAAPPQLENIDIIKKVDSRHIAQKYIELCHQ

Specific function: Adds a galactose goup to a glucose group of LPS [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI226510984, Length=217, Percent_Identity=25.3456221198157, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.4.1.- [C]

Molecular weight: Translated: 40693; Mature: 40693

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIAIFCHNFYANGTVKVALTQAEILQEAGQDVHLFVFKQEGDFPPPQNVTIHYVYKNQ
CCEEEEEEEEEECCCEEEEEEEHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEEEEECCC
KELKLKEQQQQLKRCVEAVEDSSGKFDLFLSNSTDCDTVVSGCNFDPCYYFCHCALKQEL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHCCCCCCHHHHHHHHHHHHHH
IMELKRGPIHFYRRWIKAKALIKKNIITVSEGIADELKNTGWLKAKSIQAIYNPFRIEDI
HHHHHCCHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHCCCCEEHHHHHHHHCCHHHHHH
RHKALAENDEIPNEPFMIHIGRITRQKRLDILFQALALMPSAPKLVLLTNRPERARKLAK
HHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHH
KYNVEKRIITPGFQSNPYAWIARAELMLLSSDFEGFGLVIAESLICGTPVVSTDCPYGPN
HCCCCCEEECCCCCCCCEEEEEHEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
EILTGELASYLVPRRNPKALAECAVACLAAPPQLENIDIIKKVDSRHIAQKYIELCHQ
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIAIFCHNFYANGTVKVALTQAEILQEAGQDVHLFVFKQEGDFPPPQNVTIHYVYKNQ
CCEEEEEEEEEECCCEEEEEEEHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEEEEECCC
KELKLKEQQQQLKRCVEAVEDSSGKFDLFLSNSTDCDTVVSGCNFDPCYYFCHCALKQEL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHCCCCCCHHHHHHHHHHHHHH
IMELKRGPIHFYRRWIKAKALIKKNIITVSEGIADELKNTGWLKAKSIQAIYNPFRIEDI
HHHHHCCHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHCCCCEEHHHHHHHHCCHHHHHH
RHKALAENDEIPNEPFMIHIGRITRQKRLDILFQALALMPSAPKLVLLTNRPERARKLAK
HHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHH
KYNVEKRIITPGFQSNPYAWIARAELMLLSSDFEGFGLVIAESLICGTPVVSTDCPYGPN
HCCCCCEEECCCCCCCCEEEEEHEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
EILTGELASYLVPRRNPKALAECAVACLAAPPQLENIDIIKKVDSRHIAQKYIELCHQ
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Glycosyltransferases; Hexosyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1624461; 8041620; 9278503 [H]