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The map label for this gene is kefB [H]

Identifier: 157826270

GI number: 157826270

Start: 1126654

End: 1128381

Strand: Reverse

Name: kefB [H]

Synonym: A1C_06275

Alternate gene names: 157826270

Gene position: 1128381-1126654 (Counterclockwise)

Preceding gene: 157826271

Following gene: 157826269

Centisome position: 91.66

GC content: 35.88

Gene sequence:

>1728_bases
ATGAACGATCATATTCTTATTAACGTTATAATTTTACTCGGTACTGCGGTTTTTATTGTGGCTATACTTAAGCGTCTTAG
ATTAAGTCCGGTACTTGGTTACTTAATTGCAGGAGCTGCAATAGGTGATCACGGTTTAAAAATCGTAACATATGACCAAA
CTAAGTTACTAGGTGAACTTGGTGTAGTATTTCTATTATTTGCGATAGGACTGGAGTTATCTTTTGAGCGATTAAAAGCT
ATGAGACGATATGTATTTGGTCTTGGTAGTTTGCAAGTTGTAACAACCGCTATAGTAATTGCCGGTGCAATGGTGCTTAT
AGACGGCAATAGTAGTGCAGCTATTATTACCGGCGGTGGTTTGGCCCTTTCATCTACTGCACTTGTTATGCAGGTTATTG
AGGAAAATCGTAGTCAATCGACACAAATAGGAAGAATCTCTTTGGCTATTCTGCTACTGCAAGATTTGGCTGTAGTGCCA
TTGCTTGTGATAGTTCCGTTACTTGCCGGTAATAATAAAGCCTCGCTTGCAGGGGCTCTTGGTATTGCACTCTTAAAAGC
AGTAACGGCCCTACTTACAATATTTATTGTTGGTCGTGTTTTTCTTCGTCCTGTATTTTCATTCATTTCATCAGAAAGTA
ATAATACAAGCGAGTTACCTATTTCGATGACTTTGTTAATAGTACTTTCTGCTGCTTGGGCTACCGAAACATTTGGTTTA
TCTTTAGCCTTAGGAGCATTTGTTGCTGGAGTATTAGTTGCCGAAACTGAATTTAGATTGCAGGCAGAAGAAAGTATTTA
TCCTTTCAAAAGTTTATTTTTAGGATTGTTTTTCATGACTGTCGGTATGAATATCGACGCACTAGAAATGTATGAAAAAA
TATCTCATATTCTTACTTTATCTATTGCTTTAATAGGTATAAAAACCTTAATCATAACGGCGTTTTGTCTTTTATTCGGT
TTTAATAAAGGAGTTGCTTTTTATTCAGGCTTATTATTATCGCAAGGTGGAGAGTTTGGTTTTATATTATTCAGTTTAGG
TAAAGATAGTGGAGTATTAGAAGAAAGTACTGCCGATATATTATTACTCGTAGTTACTTTTACTATGGCACTTACTCCAT
TACTCGCGGCTTTAGGGAAAAAGATTGCTGAGAAAGTTGATAAAGGACTCGGTAAAACTCCGACTCAAATGATTGAGCTT
GGGGCAAGAGATTTAACAAACCATATTATTATTGCCGGCCTTGGTAATACCGGTAAAATGGTAGCAAGAGTTTTGGAAGC
GGAAGGTATAAGTTACGTAATACTTGATTTAGATGATGATAGAGTCAAAGAAGAGCTATCAAACGGTTTGCCGGTTTTTA
AAGGTGATGTATCGCAAGCCGATACTCTAAAGGCATTAGGTACGGAAAGAGCATTCGCTATAATACTTACCATGAATAAT
CAAGTCACTATAAAGAAATCGCTTAAAACAATTAGCGGTAATTATCAGGATATACCGGTTGTCGTTAAGTTAAAAAATTT
AAAAAATGCTAGAGAGTTTTATGATTTAGGTGCCACAACTATTATTCCGGAAAGTTATGAGGCCGGATTACAAATCGGTG
GAACAGTCCTGAAGAATATAGGTATTAGCGAGCAGGAAATTAATAGAATAAAAGTACAATTTAGGCTTGGTAATTATATA
ATAGCAAAAAAAGAAGATGCTTTATCTGAAGTGGAAGATAATGATTAA

Upstream 100 bases:

>100_bases
GTAAATCAGAGTGGGTACTAATAGATGCAGGTACTGTTTTAATTAATATTTTTTATCCTGAAGCACGGGAGCATTTTAAG
CTAGAAGAAATTTGGAAAAG

Downstream 100 bases:

>100_bases
AGTTTTTATCATTTTAATTACAATAATATTATCTACAACAATTAACGCCGATAATAAAAAATTGTCTATTCCGAGATTTG
TTTCAATAAAATCTAATGAA

Product: glutathione-regulated potassium-efflux system protein kefB

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MNDHILINVIILLGTAVFIVAILKRLRLSPVLGYLIAGAAIGDHGLKIVTYDQTKLLGELGVVFLLFAIGLELSFERLKA
MRRYVFGLGSLQVVTTAIVIAGAMVLIDGNSSAAIITGGGLALSSTALVMQVIEENRSQSTQIGRISLAILLLQDLAVVP
LLVIVPLLAGNNKASLAGALGIALLKAVTALLTIFIVGRVFLRPVFSFISSESNNTSELPISMTLLIVLSAAWATETFGL
SLALGAFVAGVLVAETEFRLQAEESIYPFKSLFLGLFFMTVGMNIDALEMYEKISHILTLSIALIGIKTLIITAFCLLFG
FNKGVAFYSGLLLSQGGEFGFILFSLGKDSGVLEESTADILLLVVTFTMALTPLLAALGKKIAEKVDKGLGKTPTQMIEL
GARDLTNHIIIAGLGNTGKMVARVLEAEGISYVILDLDDDRVKEELSNGLPVFKGDVSQADTLKALGTERAFAIILTMNN
QVTIKKSLKTISGNYQDIPVVVKLKNLKNAREFYDLGATTIIPESYEAGLQIGGTVLKNIGISEQEINRIKVQFRLGNYI
IAKKEDALSEVEDND

Sequences:

>Translated_575_residues
MNDHILINVIILLGTAVFIVAILKRLRLSPVLGYLIAGAAIGDHGLKIVTYDQTKLLGELGVVFLLFAIGLELSFERLKA
MRRYVFGLGSLQVVTTAIVIAGAMVLIDGNSSAAIITGGGLALSSTALVMQVIEENRSQSTQIGRISLAILLLQDLAVVP
LLVIVPLLAGNNKASLAGALGIALLKAVTALLTIFIVGRVFLRPVFSFISSESNNTSELPISMTLLIVLSAAWATETFGL
SLALGAFVAGVLVAETEFRLQAEESIYPFKSLFLGLFFMTVGMNIDALEMYEKISHILTLSIALIGIKTLIITAFCLLFG
FNKGVAFYSGLLLSQGGEFGFILFSLGKDSGVLEESTADILLLVVTFTMALTPLLAALGKKIAEKVDKGLGKTPTQMIEL
GARDLTNHIIIAGLGNTGKMVARVLEAEGISYVILDLDDDRVKEELSNGLPVFKGDVSQADTLKALGTERAFAIILTMNN
QVTIKKSLKTISGNYQDIPVVVKLKNLKNAREFYDLGATTIIPESYEAGLQIGGTVLKNIGISEQEINRIKVQFRLGNYI
IAKKEDALSEVEDND
>Mature_575_residues
MNDHILINVIILLGTAVFIVAILKRLRLSPVLGYLIAGAAIGDHGLKIVTYDQTKLLGELGVVFLLFAIGLELSFERLKA
MRRYVFGLGSLQVVTTAIVIAGAMVLIDGNSSAAIITGGGLALSSTALVMQVIEENRSQSTQIGRISLAILLLQDLAVVP
LLVIVPLLAGNNKASLAGALGIALLKAVTALLTIFIVGRVFLRPVFSFISSESNNTSELPISMTLLIVLSAAWATETFGL
SLALGAFVAGVLVAETEFRLQAEESIYPFKSLFLGLFFMTVGMNIDALEMYEKISHILTLSIALIGIKTLIITAFCLLFG
FNKGVAFYSGLLLSQGGEFGFILFSLGKDSGVLEESTADILLLVVTFTMALTPLLAALGKKIAEKVDKGLGKTPTQMIEL
GARDLTNHIIIAGLGNTGKMVARVLEAEGISYVILDLDDDRVKEELSNGLPVFKGDVSQADTLKALGTERAFAIILTMNN
QVTIKKSLKTISGNYQDIPVVVKLKNLKNAREFYDLGATTIIPESYEAGLQIGGTVLKNIGISEQEINRIKVQFRLGNYI
IAKKEDALSEVEDND

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=383, Percent_Identity=25.8485639686684, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI1786232, Length=559, Percent_Identity=33.6314847942755, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1789749, Length=563, Percent_Identity=35.3463587921847, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1786685, Length=514, Percent_Identity=29.7665369649805, Blast_Score=155, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 61626; Mature: 61626

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDHILINVIILLGTAVFIVAILKRLRLSPVLGYLIAGAAIGDHGLKIVTYDQTKLLGEL
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHH
GVVFLLFAIGLELSFERLKAMRRYVFGLGSLQVVTTAIVIAGAMVLIDGNSSAAIITGGG
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCEEEEEECCCCEEEEECCC
LALSSTALVMQVIEENRSQSTQIGRISLAILLLQDLAVVPLLVIVPLLAGNNKASLAGAL
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
GIALLKAVTALLTIFIVGRVFLRPVFSFISSESNNTSELPISMTLLIVLSAAWATETFGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SLALGAFVAGVLVAETEFRLQAEESIYPFKSLFLGLFFMTVGMNIDALEMYEKISHILTL
HHHHHHHHHHHHHHCHHHHEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
SIALIGIKTLIITAFCLLFGFNKGVAFYSGLLLSQGGEFGFILFSLGKDSGVLEESTADI
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHH
LLLVVTFTMALTPLLAALGKKIAEKVDKGLGKTPTQMIELGARDLTNHIIIAGLGNTGKM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHCCCCEEEEECCCHHHH
VARVLEAEGISYVILDLDDDRVKEELSNGLPVFKGDVSQADTLKALGTERAFAIILTMNN
HHHHHHHCCCEEEEEECCHHHHHHHHHCCCCEEECCCCHHHHHHHHCCCCEEEEEEEECC
QVTIKKSLKTISGNYQDIPVVVKLKNLKNAREFYDLGATTIIPESYEAGLQIGGTVLKNI
CEEEHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHCCCCEECCCCHHCCHHHCHHHHHHC
GISEQEINRIKVQFRLGNYIIAKKEDALSEVEDND
CCCHHHHCEEEEEEEECCEEEEECHHHHHHCCCCC
>Mature Secondary Structure
MNDHILINVIILLGTAVFIVAILKRLRLSPVLGYLIAGAAIGDHGLKIVTYDQTKLLGEL
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHH
GVVFLLFAIGLELSFERLKAMRRYVFGLGSLQVVTTAIVIAGAMVLIDGNSSAAIITGGG
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCEEEEEECCCCEEEEECCC
LALSSTALVMQVIEENRSQSTQIGRISLAILLLQDLAVVPLLVIVPLLAGNNKASLAGAL
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
GIALLKAVTALLTIFIVGRVFLRPVFSFISSESNNTSELPISMTLLIVLSAAWATETFGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SLALGAFVAGVLVAETEFRLQAEESIYPFKSLFLGLFFMTVGMNIDALEMYEKISHILTL
HHHHHHHHHHHHHHCHHHHEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
SIALIGIKTLIITAFCLLFGFNKGVAFYSGLLLSQGGEFGFILFSLGKDSGVLEESTADI
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHH
LLLVVTFTMALTPLLAALGKKIAEKVDKGLGKTPTQMIELGARDLTNHIIIAGLGNTGKM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHCCCCEEEEECCCHHHH
VARVLEAEGISYVILDLDDDRVKEELSNGLPVFKGDVSQADTLKALGTERAFAIILTMNN
HHHHHHHCCCEEEEEECCHHHHHHHHHCCCCEEECCCCHHHHHHHHCCCCEEEEEEEECC
QVTIKKSLKTISGNYQDIPVVVKLKNLKNAREFYDLGATTIIPESYEAGLQIGGTVLKNI
CEEEHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHCCCCEECCCCHHCCHHHCHHHHHHC
GISEQEINRIKVQFRLGNYIIAKKEDALSEVEDND
CCCHHHHCEEEEEEEECCEEEEECHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA