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The map label for this gene is nth [H]

Identifier: 157826170

GI number: 157826170

Start: 1019479

End: 1020165

Strand: Reverse

Name: nth [H]

Synonym: A1C_05735

Alternate gene names: 157826170

Gene position: 1020165-1019479 (Counterclockwise)

Preceding gene: 157826183

Following gene: 157826169

Centisome position: 82.87

GC content: 32.17

Gene sequence:

>687_bases
ATGCAAGCACAAATAGTTAATAAAATTCTTGAAATTTTTAGTCAGAATAATCCAAATCCGAAAACTGAATTAATATATAA
GAATGATTTTACCTTATTAGTAGCAGTAATATTATCTGCTCAAGCCACCGATATATCAGTAAATTTAGCAACTAAATCTT
TATTTGAAGCTTACGATACACCGGAAAAAATTCTAGAACTCGGTGAAGAAGGACTTAAAAAATATATAAAATCTATCGGA
CTATTTAACAGCAAAGCAAAAAATATTATCGCATTATGTAAAATATTGATAAGCAATTATCAAGCTTCAGTACCAAATGA
CTTTCAGGAATTAGTTAAACTACCAGGCGTTGGTAGAAAAACTGCCAATGTTGTACAAAACTGCTTATTCGGTATGCCGA
CAATGGCAGTTGATACGCATGTTTTTAGAGTAGCCAAAAGGATTGGACTTGCTAAAGGTAATAGTCCTGAAATAGTAGAG
AAAGAGCTACTGCAAATTATCGATGGAAAATGGCTAACTCACGCCCATCACTGGCTAATTCTGCATGGTAGATATATTTG
CAAAGCTAGAAAACCAGATTGTGACATATGCCCTATTAAAGATGATTGTGAGTATTATATTAATAAACTCACAGTAAATG
AAATAGATATACGAAGATCAACTTCAAAAAGAGCAAGGAGTCTATAA

Upstream 100 bases:

>100_bases
ATTATTTTTTAGTGCATTTTCTATAAATTCAAAATTTTTATTTTCTAGCATTTTAAGACTCAATAATTTATTTTCTAGAA
TACACTTTCTATAGCAATAT

Downstream 100 bases:

>100_bases
GTCGAGGAACTGGGCCTATACTTAATACGTGAGTACCGCAGATCTTATAAGACGATGTAGCCAATTTTTGAAGCTCTATA
TGAGTATACATTTTAAGTTG

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MQAQIVNKILEIFSQNNPNPKTELIYKNDFTLLVAVILSAQATDISVNLATKSLFEAYDTPEKILELGEEGLKKYIKSIG
LFNSKAKNIIALCKILISNYQASVPNDFQELVKLPGVGRKTANVVQNCLFGMPTMAVDTHVFRVAKRIGLAKGNSPEIVE
KELLQIIDGKWLTHAHHWLILHGRYICKARKPDCDICPIKDDCEYYINKLTVNEIDIRRSTSKRARSL

Sequences:

>Translated_228_residues
MQAQIVNKILEIFSQNNPNPKTELIYKNDFTLLVAVILSAQATDISVNLATKSLFEAYDTPEKILELGEEGLKKYIKSIG
LFNSKAKNIIALCKILISNYQASVPNDFQELVKLPGVGRKTANVVQNCLFGMPTMAVDTHVFRVAKRIGLAKGNSPEIVE
KELLQIIDGKWLTHAHHWLILHGRYICKARKPDCDICPIKDDCEYYINKLTVNEIDIRRSTSKRARSL
>Mature_228_residues
MQAQIVNKILEIFSQNNPNPKTELIYKNDFTLLVAVILSAQATDISVNLATKSLFEAYDTPEKILELGEEGLKKYIKSIG
LFNSKAKNIIALCKILISNYQASVPNDFQELVKLPGVGRKTANVVQNCLFGMPTMAVDTHVFRVAKRIGLAKGNSPEIVE
KELLQIIDGKWLTHAHHWLILHGRYICKARKPDCDICPIKDDCEYYINKLTVNEIDIRRSTSKRARSL

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=171, Percent_Identity=30.9941520467836, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1787920, Length=198, Percent_Identity=56.0606060606061, Blast_Score=231, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI17554540, Length=194, Percent_Identity=31.4432989690722, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319304, Length=183, Percent_Identity=30.0546448087432, Blast_Score=69, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324530, Length=199, Percent_Identity=27.6381909547739, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=30.5555555555556, Blast_Score=84, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 25726; Mature: 25726

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAQIVNKILEIFSQNNPNPKTELIYKNDFTLLVAVILSAQATDISVNLATKSLFEAYDT
CCHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCC
PEKILELGEEGLKKYIKSIGLFNSKAKNIIALCKILISNYQASVPNDFQELVKLPGVGRK
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCH
TANVVQNCLFGMPTMAVDTHVFRVAKRIGLAKGNSPEIVEKELLQIIDGKWLTHAHHWLI
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHEEEE
LHGRYICKARKPDCDICPIKDDCEYYINKLTVNEIDIRRSTSKRARSL
EECCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQAQIVNKILEIFSQNNPNPKTELIYKNDFTLLVAVILSAQATDISVNLATKSLFEAYDT
CCHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCC
PEKILELGEEGLKKYIKSIGLFNSKAKNIIALCKILISNYQASVPNDFQELVKLPGVGRK
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCH
TANVVQNCLFGMPTMAVDTHVFRVAKRIGLAKGNSPEIVEKELLQIIDGKWLTHAHHWLI
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHEEEE
LHGRYICKARKPDCDICPIKDDCEYYINKLTVNEIDIRRSTSKRARSL
EECCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA