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The map label for this gene is trmE

Identifier: 157826149

GI number: 157826149

Start: 1001597

End: 1002934

Strand: Reverse

Name: trmE

Synonym: A1C_05630

Alternate gene names: 157826149

Gene position: 1002934-1001597 (Counterclockwise)

Preceding gene: 157826157

Following gene: 157826148

Centisome position: 81.47

GC content: 33.33

Gene sequence:

>1338_bases
GTGGAAACTATATTTGCACAAAGCTCTGCATTCGGTAAAGCAGGGGTAGCGGTTTTTAGGATTTCAGGTCCTAAAAGTCT
GGAAGTTTTGCAGCTGCTTACTGGTAAAAAAGCCTTTAAGCCGAGATTAATGTATTACCAACAAATTATTGCCCCTGAAA
CCAAGGAGCTAATTGATAATGCTATGGTTGTTTATTTTAAGCTACCTAATAGTTTTACTGGAGAAGACGTAGTCGAGATT
CATACTCACGGCAGTAAAGCTATATCTATTATGATGATAAATACTTTGTTAAATATCTCTGATATTCGTTTGGCAGAAGC
AGGGGAATTTACCAAAAGAGCTTTTTTAAATAATAAATTTGATTTAACAGCCGCAGAGGGAATAGCTGATCTAATTAACG
CCGAAACCATTATGCAGCATAGGCAAGCAATTAGACAAGCAAGTGGAAGACTTGAAGAATTATATAATAGCTGGCGTAGT
CAGCTTTTAAAAATTATCTCTCTGCTTGAAGCTTATATAGATTTCCCTGATGAAGATATACCGGATAGCGTCCTTAACGA
CGTTAATAATACTCATAAAAACCTTGTAAATGAAATATCTAATTATCTTAACGATAATAGACGAGGAGAATTACTAAATA
GCGGTCTTAAACTTGCAATTGTTGGTCCACCTAATACAGGTAAGTCTAGCTTATTAAACTTCTTAATGCAAAGAGACATA
GCGATGGTCTCAAATATTGCAGGCACTACTAGGGATATAATAGAGGGTCATTTAGACATTGGCGGTTATCCTATTATTTT
ACAAGATACAGCAGGTATAAGAGAAGAAAGTAGTGACATTATCGAGCAAGAAGGTATAAAAAGAGCTATTAATTCGGCTA
AAACAGCGGATATTAAAATTATTATGTTTGATGCCAAAAAATTAGATTCCTCTATAAATGAAGATATTACAGGTTTAATT
GATGAAAATACTATCGTAATAATTAATAAAATAGACCTTATAGAACCAAGCAAAGTATTTTCAATTGAAGATAAATATAA
ATGCTTAAGAGTTTCGGTAAAAAATAATATTGCTCTTTCAAGTATATTAAAAAATATTGAAAATATTGCCGAAAATATGG
CGGGCTTTACCGAAACGCCTTACATAACAAATCAACGTCATCGTCATTATTTAAAACAAGCTCTTTCACATTTAACGGAT
TTTAGCTTAGATAATGATTTAGTTCTAGCAACTGAAGATATTAGAATTACGGCACGCTGCATTGGTGCTATTACGGGCGT
TATTAACGTAGAAGAAATACTAGGCGAGATCTTTCAAAATTTCTGCATAGGGAAATAA

Upstream 100 bases:

>100_bases
AAGTTTAAAGGAGTTGTTTTGTATGCCATGATTTTCTCTAGAATAATAATAAAGTTATTATATTATATAAAATAACTTAA
GCAAGAGGCATAGAAAATAA

Downstream 100 bases:

>100_bases
TGTATATGTGATGCCAGCTTGGCATTGGTATTATTGCATGGATCAAAAAACGCACTTGGTGTCACATACCGTAGCTTGAC
CACGGGATCTAGTTAAAAAT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
METIFAQSSAFGKAGVAVFRISGPKSLEVLQLLTGKKAFKPRLMYYQQIIAPETKELIDNAMVVYFKLPNSFTGEDVVEI
HTHGSKAISIMMINTLLNISDIRLAEAGEFTKRAFLNNKFDLTAAEGIADLINAETIMQHRQAIRQASGRLEELYNSWRS
QLLKIISLLEAYIDFPDEDIPDSVLNDVNNTHKNLVNEISNYLNDNRRGELLNSGLKLAIVGPPNTGKSSLLNFLMQRDI
AMVSNIAGTTRDIIEGHLDIGGYPIILQDTAGIREESSDIIEQEGIKRAINSAKTADIKIIMFDAKKLDSSINEDITGLI
DENTIVIINKIDLIEPSKVFSIEDKYKCLRVSVKNNIALSSILKNIENIAENMAGFTETPYITNQRHRHYLKQALSHLTD
FSLDNDLVLATEDIRITARCIGAITGVINVEEILGEIFQNFCIGK

Sequences:

>Translated_445_residues
METIFAQSSAFGKAGVAVFRISGPKSLEVLQLLTGKKAFKPRLMYYQQIIAPETKELIDNAMVVYFKLPNSFTGEDVVEI
HTHGSKAISIMMINTLLNISDIRLAEAGEFTKRAFLNNKFDLTAAEGIADLINAETIMQHRQAIRQASGRLEELYNSWRS
QLLKIISLLEAYIDFPDEDIPDSVLNDVNNTHKNLVNEISNYLNDNRRGELLNSGLKLAIVGPPNTGKSSLLNFLMQRDI
AMVSNIAGTTRDIIEGHLDIGGYPIILQDTAGIREESSDIIEQEGIKRAINSAKTADIKIIMFDAKKLDSSINEDITGLI
DENTIVIINKIDLIEPSKVFSIEDKYKCLRVSVKNNIALSSILKNIENIAENMAGFTETPYITNQRHRHYLKQALSHLTD
FSLDNDLVLATEDIRITARCIGAITGVINVEEILGEIFQNFCIGK
>Mature_445_residues
METIFAQSSAFGKAGVAVFRISGPKSLEVLQLLTGKKAFKPRLMYYQQIIAPETKELIDNAMVVYFKLPNSFTGEDVVEI
HTHGSKAISIMMINTLLNISDIRLAEAGEFTKRAFLNNKFDLTAAEGIADLINAETIMQHRQAIRQASGRLEELYNSWRS
QLLKIISLLEAYIDFPDEDIPDSVLNDVNNTHKNLVNEISNYLNDNRRGELLNSGLKLAIVGPPNTGKSSLLNFLMQRDI
AMVSNIAGTTRDIIEGHLDIGGYPIILQDTAGIREESSDIIEQEGIKRAINSAKTADIKIIMFDAKKLDSSINEDITGLI
DENTIVIINKIDLIEPSKVFSIEDKYKCLRVSVKNNIALSSILKNIENIAENMAGFTETPYITNQRHRHYLKQALSHLTD
FSLDNDLVLATEDIRITARCIGAITGVINVEEILGEIFQNFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=459, Percent_Identity=38.1263616557734, Blast_Score=315, Evalue=4e-86,
Organism=Homo sapiens, GI193082993, Length=447, Percent_Identity=38.0313199105145, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI193082988, Length=491, Percent_Identity=35.8452138492872, Blast_Score=303, Evalue=3e-82,
Organism=Escherichia coli, GI2367268, Length=459, Percent_Identity=34.8583877995643, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI87082120, Length=122, Percent_Identity=31.1475409836066, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17507259, Length=451, Percent_Identity=32.8159645232816, Blast_Score=221, Evalue=7e-58,
Organism=Saccharomyces cerevisiae, GI6323665, Length=491, Percent_Identity=36.8635437881874, Blast_Score=265, Evalue=1e-71,
Organism=Drosophila melanogaster, GI45550813, Length=470, Percent_Identity=37.6595744680851, Blast_Score=327, Evalue=8e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_RICAH (A8GPN6)

Other databases:

- EMBL:   CP000847
- RefSeq:   YP_001493869.1
- ProteinModelPortal:   A8GPN6
- SMR:   A8GPN6
- STRING:   A8GPN6
- GeneID:   5644757
- GenomeReviews:   CP000847_GR
- KEGG:   rak:A1C_05630
- NMPDR:   fig|293614.3.peg.1060
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   LIDNAMV
- ProtClustDB:   PRK05291
- BioCyc:   RAKA293614:A1C_05630-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 49589; Mature: 49589

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: BINDING 20-20 BINDING 79-79 BINDING 119-119 BINDING 445-445

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METIFAQSSAFGKAGVAVFRISGPKSLEVLQLLTGKKAFKPRLMYYQQIIAPETKELIDN
CCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCHHCCHHHHHHHHHHCCCHHHHHCC
AMVVYFKLPNSFTGEDVVEIHTHGSKAISIMMINTLLNISDIRLAEAGEFTKRAFLNNKF
CEEEEEECCCCCCCCCEEEEECCCCCEEEEEEHHHHHCHHHCEEHHCCHHHHHHHHCCCC
DLTAAEGIADLINAETIMQHRQAIRQASGRLEELYNSWRSQLLKIISLLEAYIDFPDEDI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PDSVLNDVNNTHKNLVNEISNYLNDNRRGELLNSGLKLAIVGPPNTGKSSLLNFLMQRDI
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
AMVSNIAGTTRDIIEGHLDIGGYPIILQDTAGIREESSDIIEQEGIKRAINSAKTADIKI
HHHHHHCCCHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
IMFDAKKLDSSINEDITGLIDENTIVIINKIDLIEPSKVFSIEDKYKCLRVSVKNNIALS
EEEECHHHHHHHCCCHHEEECCCEEEEEEEECCCCCCCEEECCCCCEEEEEECCCCHHHH
SILKNIENIAENMAGFTETPYITNQRHRHYLKQALSHLTDFSLDNDLVLATEDIRITARC
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHH
IGAITGVINVEEILGEIFQNFCIGK
HHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
METIFAQSSAFGKAGVAVFRISGPKSLEVLQLLTGKKAFKPRLMYYQQIIAPETKELIDN
CCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCHHCCHHHHHHHHHHCCCHHHHHCC
AMVVYFKLPNSFTGEDVVEIHTHGSKAISIMMINTLLNISDIRLAEAGEFTKRAFLNNKF
CEEEEEECCCCCCCCCEEEEECCCCCEEEEEEHHHHHCHHHCEEHHCCHHHHHHHHCCCC
DLTAAEGIADLINAETIMQHRQAIRQASGRLEELYNSWRSQLLKIISLLEAYIDFPDEDI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PDSVLNDVNNTHKNLVNEISNYLNDNRRGELLNSGLKLAIVGPPNTGKSSLLNFLMQRDI
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
AMVSNIAGTTRDIIEGHLDIGGYPIILQDTAGIREESSDIIEQEGIKRAINSAKTADIKI
HHHHHHCCCHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
IMFDAKKLDSSINEDITGLIDENTIVIINKIDLIEPSKVFSIEDKYKCLRVSVKNNIALS
EEEECHHHHHHHCCCHHEEECCCEEEEEEEECCCCCCCEEECCCCCEEEEEECCCCHHHH
SILKNIENIAENMAGFTETPYITNQRHRHYLKQALSHLTDFSLDNDLVLATEDIRITARC
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHH
IGAITGVINVEEILGEIFQNFCIGK
HHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA