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The map label for this gene is tuf

Identifier: 157826065

GI number: 157826065

Start: 914013

End: 915200

Strand: Reverse

Name: tuf

Synonym: A1C_05115

Alternate gene names: 157826065

Gene position: 915200-914013 (Counterclockwise)

Preceding gene: 157826074

Following gene: 157826064

Centisome position: 74.34

GC content: 39.56

Gene sequence:

>1188_bases
ATGGCAAAAGCAAAATTTGAACGAACTAAACCGCACGTTAATATAGGTACGATCGGTCATGTAGATCACGGTAAGACTTC
CTTAACGGCAGCAATAACTACAGTACTTGCTAAGACAGGTGGAGCAAAAGCTACAGCGTATGATCAAATTGACGCTGCTC
CTGAAGAAAAAGAAAGAGGCATAACTATTTCTACCGCACACGTAGAGTATGAGACTAAAAATAGACACTATGCACACGTA
GATTGTCCGGGACACGCTGACTATATAAAGAACATGATAACCGGTGCCGCTCAGATGGATGGTGCAATATTAGTAGTTTC
TGCTGCTGATGGTCCAATGCTGCAAACTAGAGAACATATATTACTAGCAAAACAGGTAGGTGTACCTGCTATGGTGGTAT
TCTTAAATAAAGTAGATGTGGTGGATGATCCTGCTCTATTAGAATTAGTAGAGATGGAAGTAAGGGACTTATTATTACAA
TACGGTTTTCCGGCTTATGAAGTACCTATTATTAAAGGTTCTGCGCTTCAGGCCTTAGCAGGAAAGCCTGAAGGTGAAAA
AGCTATTAATGAGTTAATGGATGCAGTAGATAATTATATACCGCATCCCGTAAGAGCTACTGATAAACCCTTCTTAATGC
CGATAGAAGATGTATTCTCGATTTCAGGAAGAGGTACTGTTGTAACTGGTAGAGTAGAGTCAGGAATAATTAAGGTAGGT
GAGGAAGTTGAAATAGTCGGTTTAAAAGAGACACAAAAAACTACCTGTACCGGTGTAGAAATGTTCAGGAAATTACTTGA
TGAAGGACAAGCCGGAGATAATGTCGGTATATTACTACGTGGTACAAAAAGGGAAGAAGTTGAAAGAGGACAAGTTCTCG
CAAAACCTGGTAGCATAAAACCGCATGATCAGTTTGAAGCTGAAGTATATGTGCTTAGTAAAGAAGAAGGTGGACGTCAT
ACACCATTTACTAATGACTATCGTCCGCAGTTTTATTTTAGAACGACAGACGTTACCGGTACAATAAAATTGCCTGCTGA
TAAGCAGATGGTGATGCCTGGAGATAATGCTACTACTTTTACAGTGGAATTAATTAAACCAATTGCTATGCAGCAAGGGT
CAAAATTCTCTATACGTGAAGGTGGTAAAACAGTGGGTGCCGGCGTAGTAACTAAAATAAATAATTAA

Upstream 100 bases:

>100_bases
GGGTTCGATTCCCATCACCCGCTCCAGAGATAGTGGTAATAAATAAATTTAGAAAAAAATTAACTTGTATTAAACCTAAA
AAATTGTAGAGAGTAACAAT

Downstream 100 bases:

>100_bases
TTTATTTTATAAATACCTATTATTTTTTAATAATGGGTATTTATATTTTAAGATGTCTGCAACACATCTGGCTGTTTAAA
TATTAGGTTTACTGAATGAA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MAKAKFERTKPHVNIGTIGHVDHGKTSLTAAITTVLAKTGGAKATAYDQIDAAPEEKERGITISTAHVEYETKNRHYAHV
DCPGHADYIKNMITGAAQMDGAILVVSAADGPMLQTREHILLAKQVGVPAMVVFLNKVDVVDDPALLELVEMEVRDLLLQ
YGFPAYEVPIIKGSALQALAGKPEGEKAINELMDAVDNYIPHPVRATDKPFLMPIEDVFSISGRGTVVTGRVESGIIKVG
EEVEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGILLRGTKREEVERGQVLAKPGSIKPHDQFEAEVYVLSKEEGGRH
TPFTNDYRPQFYFRTTDVTGTIKLPADKQMVMPGDNATTFTVELIKPIAMQQGSKFSIREGGKTVGAGVVTKINN

Sequences:

>Translated_395_residues
MAKAKFERTKPHVNIGTIGHVDHGKTSLTAAITTVLAKTGGAKATAYDQIDAAPEEKERGITISTAHVEYETKNRHYAHV
DCPGHADYIKNMITGAAQMDGAILVVSAADGPMLQTREHILLAKQVGVPAMVVFLNKVDVVDDPALLELVEMEVRDLLLQ
YGFPAYEVPIIKGSALQALAGKPEGEKAINELMDAVDNYIPHPVRATDKPFLMPIEDVFSISGRGTVVTGRVESGIIKVG
EEVEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGILLRGTKREEVERGQVLAKPGSIKPHDQFEAEVYVLSKEEGGRH
TPFTNDYRPQFYFRTTDVTGTIKLPADKQMVMPGDNATTFTVELIKPIAMQQGSKFSIREGGKTVGAGVVTKINN
>Mature_394_residues
AKAKFERTKPHVNIGTIGHVDHGKTSLTAAITTVLAKTGGAKATAYDQIDAAPEEKERGITISTAHVEYETKNRHYAHVD
CPGHADYIKNMITGAAQMDGAILVVSAADGPMLQTREHILLAKQVGVPAMVVFLNKVDVVDDPALLELVEMEVRDLLLQY
GFPAYEVPIIKGSALQALAGKPEGEKAINELMDAVDNYIPHPVRATDKPFLMPIEDVFSISGRGTVVTGRVESGIIKVGE
EVEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGILLRGTKREEVERGQVLAKPGSIKPHDQFEAEVYVLSKEEGGRHT
PFTNDYRPQFYFRTTDVTGTIKLPADKQMVMPGDNATTFTVELIKPIAMQQGSKFSIREGGKTVGAGVVTKINN

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=392, Percent_Identity=56.6326530612245, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI4503475, Length=453, Percent_Identity=29.8013245033113, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI4503471, Length=453, Percent_Identity=29.3598233995585, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI223555963, Length=444, Percent_Identity=29.0540540540541, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI5729864, Length=444, Percent_Identity=29.0540540540541, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI54607086, Length=319, Percent_Identity=30.4075235109718, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI194018522, Length=443, Percent_Identity=26.410835214447, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI194018520, Length=443, Percent_Identity=26.410835214447, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI194097354, Length=443, Percent_Identity=26.410835214447, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI46094014, Length=443, Percent_Identity=26.410835214447, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI4503507, Length=268, Percent_Identity=29.4776119402985, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI82546879, Length=428, Percent_Identity=24.0654205607477, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1790412, Length=393, Percent_Identity=76.5903307888041, Blast_Score=591, Evalue=1e-170,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=76.5903307888041, Blast_Score=591, Evalue=1e-170,
Organism=Escherichia coli, GI2367247, Length=339, Percent_Identity=30.3834808259587, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI48994988, Length=281, Percent_Identity=31.6725978647687, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789108, Length=267, Percent_Identity=27.7153558052434, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788922, Length=151, Percent_Identity=31.1258278145695, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=51.3784461152882, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25141371, Length=388, Percent_Identity=44.5876288659794, Blast_Score=336, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=450, Percent_Identity=30.6666666666667, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17569207, Length=450, Percent_Identity=30.6666666666667, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI32566303, Length=431, Percent_Identity=29.2343387470998, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=433, Percent_Identity=27.2517321016166, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=430, Percent_Identity=27.2093023255814, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=456, Percent_Identity=25, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=271, Percent_Identity=29.1512915129151, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI32566301, Length=148, Percent_Identity=37.8378378378378, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=266, Percent_Identity=27.4436090225564, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=26.8398268398268, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17563834, Length=430, Percent_Identity=23.7209302325581, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=64.9874055415617, Blast_Score=515, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6325337, Length=450, Percent_Identity=30.8888888888889, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=450, Percent_Identity=30.8888888888889, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=349, Percent_Identity=26.9340974212034, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320377, Length=341, Percent_Identity=27.8592375366569, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=275, Percent_Identity=26.5454545454545, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=256, Percent_Identity=27.734375, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=175, Percent_Identity=28, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI281363316, Length=391, Percent_Identity=58.5677749360614, Blast_Score=476, Evalue=1e-134,
Organism=Drosophila melanogaster, GI17864358, Length=391, Percent_Identity=58.5677749360614, Blast_Score=476, Evalue=1e-134,
Organism=Drosophila melanogaster, GI19921738, Length=387, Percent_Identity=49.6124031007752, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45553807, Length=456, Percent_Identity=30.9210526315789, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI45553816, Length=456, Percent_Identity=30.9210526315789, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24651721, Length=456, Percent_Identity=30.9210526315789, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI17864154, Length=456, Percent_Identity=30.9210526315789, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24652838, Length=453, Percent_Identity=30.242825607064, Blast_Score=168, Evalue=5e-42,
Organism=Drosophila melanogaster, GI17137572, Length=453, Percent_Identity=30.242825607064, Blast_Score=168, Evalue=5e-42,
Organism=Drosophila melanogaster, GI45550900, Length=434, Percent_Identity=26.9585253456221, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17137380, Length=341, Percent_Identity=30.791788856305, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=30.6930693069307, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24647046, Length=358, Percent_Identity=26.8156424581006, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24647048, Length=358, Percent_Identity=26.8156424581006, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI78706572, Length=305, Percent_Identity=27.5409836065574, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582462, Length=146, Percent_Identity=32.1917808219178, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI28572034, Length=173, Percent_Identity=34.6820809248555, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_RICAH (A8GPF2)

Other databases:

- EMBL:   CP000847
- RefSeq:   YP_001493785.1
- ProteinModelPortal:   A8GPF2
- SMR:   A8GPF2
- STRING:   A8GPF2
- GeneID:   5645309
- GenomeReviews:   CP000847_GR
- KEGG:   rak:A1C_05115
- NMPDR:   fig|293614.3.peg.963
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- ProtClustDB:   PRK00049
- BioCyc:   RAKA293614:A1C_05115-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 42891; Mature: 42760

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHVNIGTIGHVDHGKTSLTAAITTVLAKTGGAKATAYDQIDAAPEEKERG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCEEHHHCCCCCCHHHCC
ITISTAHVEYETKNRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMLQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCHHHHHHE
LLAKQVGVPAMVVFLNKVDVVDDPALLELVEMEVRDLLLQYGFPAYEVPIIKGSALQALA
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHC
GKPEGEKAINELMDAVDNYIPHPVRATDKPFLMPIEDVFSISGRGTVVTGRVESGIIKVG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHCCCCCCEEEECCCCCCEEECC
EEVEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGILLRGTKREEVERGQVLAKPGSIK
CEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHCCCEEECCCCCC
PHDQFEAEVYVLSKEEGGRHTPFTNDYRPQFYFRTTDVTGTIKLPADKQMVMPGDNATTF
CCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCCEEE
TVELIKPIAMQQGSKFSIREGGKTVGAGVVTKINN
EEEEHHHHHHCCCCEEEECCCCCEECCEEEEEECC
>Mature Secondary Structure 
AKAKFERTKPHVNIGTIGHVDHGKTSLTAAITTVLAKTGGAKATAYDQIDAAPEEKERG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCEEHHHCCCCCCHHHCC
ITISTAHVEYETKNRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMLQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCHHHHHHE
LLAKQVGVPAMVVFLNKVDVVDDPALLELVEMEVRDLLLQYGFPAYEVPIIKGSALQALA
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHC
GKPEGEKAINELMDAVDNYIPHPVRATDKPFLMPIEDVFSISGRGTVVTGRVESGIIKVG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHCCCCCCEEEECCCCCCEEECC
EEVEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGILLRGTKREEVERGQVLAKPGSIK
CEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHCCCEEECCCCCC
PHDQFEAEVYVLSKEEGGRHTPFTNDYRPQFYFRTTDVTGTIKLPADKQMVMPGDNATTF
CCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCCEEE
TVELIKPIAMQQGSKFSIREGGKTVGAGVVTKINN
EEEEHHHHHHCCCCEEEECCCCCEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA