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The map label for this gene is murF [H]

Identifier: 157825983

GI number: 157825983

Start: 839258

End: 840607

Strand: Reverse

Name: murF [H]

Synonym: A1C_04665

Alternate gene names: 157825983

Gene position: 840607-839258 (Counterclockwise)

Preceding gene: 157825984

Following gene: 157825982

Centisome position: 68.28

GC content: 30.44

Gene sequence:

>1350_bases
ATGATCTGGAACTCTAAAACTTTAAGTTTTGCTCTTGGTATAACAATCTCTAATTCTATCAATTGTAATGAGGTACAATT
AAACTCTAAAGATGTTAAAAAAGGTGACTTATTTATAGCACTTAAAGGTAATAGAGACGGTCATGATTATGCTCTAGATG
CTATAGATAAAGGGGCGGCAGCCGTAATTGTTAGTAAGCAAGTAGAGATAACAGATAAAGATAAAATTCTCTTAGTCGAT
GATTGTTTTGAAGCTCTAAAAAAAATGGCTCTATATAAACGAGAGAATTCAAAAGCTAAATTTATAGCTATAACTGGTAG
TGTCGGTAAAACTTCCACTAAAGAAGCATTAAAAATATTATTACAACATGATTCGTTAGTTTTTGCAGGTAGAGGCAACT
TTAATAACTATTTGGGGTTACTTATTAATCTTGCATCAATGGCTGATGATATGGAATATGCTATTCTGGAGCTTGGTATG
AACCATAAAGGCGAGATAAGGGAACTGGTTCAAATATTAAAGCCGAATATTGCTGTGATTACTAATATTTCGGAAGCTCA
TTTAGAGTTTTTTAACTCGCTTGAAGAGATAGCTGAAGCTAAATGTGAGATTTTTGAAAATTTTAGCAAAAATGATATTG
CTGTTATTAATGCCGATACTAATTGTTATAGCAAAATATTATCGATATTAAAAAACCTTTATATTGATAGGGTATATAAC
TTCGGCATATTGCATAATACACATGCACAATTAGTTTTATATGAGCACCTAGGAGCGCAAGTCCGCTTAAAATATAAGAT
AAATAATAAATCCTTAGAGATCACTATACCGTTCATTCCTAGGCATTTTGCAAAAAATTACACCGTGGTACTTTTAATTA
TCGATATATTAGGTAAAGATATAGAGATAGCTGCAAATCACTTAGCAAATATTTATCCTACTAAAGGAAGAGGGGATATT
ATCAATATACAAAATGCTCGTGTAATTTGTGATTACTATAACGCAAGTCCCAAGTCAATGCAAGCTGCATTAGAATATTT
AAAGCAAGTAATGGCTGAGAATAAAACCGCTATAATAGGCGATATGCTAGAGTTAGGGAAAAATTCTAAACTGTTACATG
AGGAACTAGTGCCTTATATATTAGATGTCGGTTGTTCTAAGATCTATTTAGTAGGAGTAAATACTAAATATATTTATGAT
TTACTTCCTTCCAAGATAGCTAAAAAATACTTTAAAAATGTTGATGAATTAATTACACATATTACGGATTTATTTAAAGG
TAATGATCTTATCTTAATAAAAGGTTCAAGAGGTCTAAAACTTGATAAGATTGTTGATTATTATAAATAA

Upstream 100 bases:

>100_bases
TTACAGTCGCTCGCAATGACGGGTTTGGCATCTACGTGGGCAATGCCATGCGGGCATGACATAAACAAGCCATACAACAA
CACATAAATAACAAAAACAT

Downstream 100 bases:

>100_bases
ATACACTAGGATATTTTTAATGTTGTGTGGTTTGGTACAATGGTTGTCATCCTGCGACTTGATCGCGGTATGACATCGAG
TGCGTTTTTCTAGCTATACA

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MIWNSKTLSFALGITISNSINCNEVQLNSKDVKKGDLFIALKGNRDGHDYALDAIDKGAAAVIVSKQVEITDKDKILLVD
DCFEALKKMALYKRENSKAKFIAITGSVGKTSTKEALKILLQHDSLVFAGRGNFNNYLGLLINLASMADDMEYAILELGM
NHKGEIRELVQILKPNIAVITNISEAHLEFFNSLEEIAEAKCEIFENFSKNDIAVINADTNCYSKILSILKNLYIDRVYN
FGILHNTHAQLVLYEHLGAQVRLKYKINNKSLEITIPFIPRHFAKNYTVVLLIIDILGKDIEIAANHLANIYPTKGRGDI
INIQNARVICDYYNASPKSMQAALEYLKQVMAENKTAIIGDMLELGKNSKLLHEELVPYILDVGCSKIYLVGVNTKYIYD
LLPSKIAKKYFKNVDELITHITDLFKGNDLILIKGSRGLKLDKIVDYYK

Sequences:

>Translated_449_residues
MIWNSKTLSFALGITISNSINCNEVQLNSKDVKKGDLFIALKGNRDGHDYALDAIDKGAAAVIVSKQVEITDKDKILLVD
DCFEALKKMALYKRENSKAKFIAITGSVGKTSTKEALKILLQHDSLVFAGRGNFNNYLGLLINLASMADDMEYAILELGM
NHKGEIRELVQILKPNIAVITNISEAHLEFFNSLEEIAEAKCEIFENFSKNDIAVINADTNCYSKILSILKNLYIDRVYN
FGILHNTHAQLVLYEHLGAQVRLKYKINNKSLEITIPFIPRHFAKNYTVVLLIIDILGKDIEIAANHLANIYPTKGRGDI
INIQNARVICDYYNASPKSMQAALEYLKQVMAENKTAIIGDMLELGKNSKLLHEELVPYILDVGCSKIYLVGVNTKYIYD
LLPSKIAKKYFKNVDELITHITDLFKGNDLILIKGSRGLKLDKIVDYYK
>Mature_449_residues
MIWNSKTLSFALGITISNSINCNEVQLNSKDVKKGDLFIALKGNRDGHDYALDAIDKGAAAVIVSKQVEITDKDKILLVD
DCFEALKKMALYKRENSKAKFIAITGSVGKTSTKEALKILLQHDSLVFAGRGNFNNYLGLLINLASMADDMEYAILELGM
NHKGEIRELVQILKPNIAVITNISEAHLEFFNSLEEIAEAKCEIFENFSKNDIAVINADTNCYSKILSILKNLYIDRVYN
FGILHNTHAQLVLYEHLGAQVRLKYKINNKSLEITIPFIPRHFAKNYTVVLLIIDILGKDIEIAANHLANIYPTKGRGDI
INIQNARVICDYYNASPKSMQAALEYLKQVMAENKTAIIGDMLELGKNSKLLHEELVPYILDVGCSKIYLVGVNTKYIYD
LLPSKIAKKYFKNVDELITHITDLFKGNDLILIKGSRGLKLDKIVDYYK

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=430, Percent_Identity=30.6976744186047, Blast_Score=178, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50474; Mature: 50474

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWNSKTLSFALGITISNSINCNEVQLNSKDVKKGDLFIALKGNRDGHDYALDAIDKGAA
CCCCCCEEEEEEEEEEECCCCCCEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHCCCCE
AVIVSKQVEITDKDKILLVDDCFEALKKMALYKRENSKAKFIAITGSVGKTSTKEALKIL
EEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHH
LQHDSLVFAGRGNFNNYLGLLINLASMADDMEYAILELGMNHKGEIRELVQILKPNIAVI
HHCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHCCCEEEE
TNISEAHLEFFNSLEEIAEAKCEIFENFSKNDIAVINADTNCYSKILSILKNLYIDRVYN
ECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
FGILHNTHAQLVLYEHLGAQVRLKYKINNKSLEITIPFIPRHFAKNYTVVLLIIDILGKD
CCEEECCCCEEEEEECCCCEEEEEEEECCCEEEEEECCCCHHHCCCCEEEEEEEHHHCCC
IEIAANHLANIYPTKGRGDIINIQNARVICDYYNASPKSMQAALEYLKQVMAENKTAIIG
HHHHHHHHHHCCCCCCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEH
DMLELGKNSKLLHEELVPYILDVGCSKIYLVGVNTKYIYDLLPSKIAKKYFKNVDELITH
HHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHH
ITDLFKGNDLILIKGSRGLKLDKIVDYYK
HHHHHCCCCEEEEECCCCCCHHHHHHHCC
>Mature Secondary Structure
MIWNSKTLSFALGITISNSINCNEVQLNSKDVKKGDLFIALKGNRDGHDYALDAIDKGAA
CCCCCCEEEEEEEEEEECCCCCCEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHCCCCE
AVIVSKQVEITDKDKILLVDDCFEALKKMALYKRENSKAKFIAITGSVGKTSTKEALKIL
EEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHH
LQHDSLVFAGRGNFNNYLGLLINLASMADDMEYAILELGMNHKGEIRELVQILKPNIAVI
HHCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHCCCEEEE
TNISEAHLEFFNSLEEIAEAKCEIFENFSKNDIAVINADTNCYSKILSILKNLYIDRVYN
ECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
FGILHNTHAQLVLYEHLGAQVRLKYKINNKSLEITIPFIPRHFAKNYTVVLLIIDILGKD
CCEEECCCCEEEEEECCCCEEEEEEEECCCEEEEEECCCCHHHCCCCEEEEEEEHHHCCC
IEIAANHLANIYPTKGRGDIINIQNARVICDYYNASPKSMQAALEYLKQVMAENKTAIIG
HHHHHHHHHHCCCCCCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEH
DMLELGKNSKLLHEELVPYILDVGCSKIYLVGVNTKYIYDLLPSKIAKKYFKNVDELITH
HHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHH
ITDLFKGNDLILIKGSRGLKLDKIVDYYK
HHHHHCCCCEEEEECCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]