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The map label for this gene is odhL [H]

Identifier: 157825888

GI number: 157825888

Start: 746259

End: 747638

Strand: Reverse

Name: odhL [H]

Synonym: A1C_04135

Alternate gene names: 157825888

Gene position: 747638-746259 (Counterclockwise)

Preceding gene: 157825889

Following gene: 157825887

Centisome position: 60.73

GC content: 39.2

Gene sequence:

>1380_bases
ATGCAAGAATTTGATTTAGTGGTAATAGGTAGCGGTCCGGCCGGTTATACAGGTAGTATTAGAGCTGCTCAGCTTGGTAT
GAAAGTAGTGTGTGTTGAGAAAAATGATACTCTCGGTGGCACTTGTTTAAATATAGGATGCATACCGTCGAAAGCTTTAC
TTAATTCCTCTGAAAAATATGAAGAAGCTTTAAAGCATTTTGAGAGTATAGGTATTACTGCGGATGTAAAATTAGACTTA
CAAAAAATGATAGCTAATAAAGATAAAGTAGTTTCTGACCTTACAAAAGGGATAGAAAGCTTATTTGCTAAGAATAAGAT
TACTAGGATAAAGGGTGAAGCTAAAATTATCGCGAGCAACATTGTGGAAGTAAATAATGAGCAAATTAAAGCCAAAAATA
TCCTAATTACTACGGGTTCCAGCGTCATAGAAATACCAAATATCAAAATAGATGAAGAGTTTATAGTTTCATCTACCGGA
GCTTTAAAGCTTTCTAAAGTACCTGAGACCTTAATAGTTGTCGGGGGAGGATATATAGGCTTAGAACTCGGCTCAGTTTG
GCGTCGTTTAGGTGCTAAAGTAACGGTTATTGAGTATGCACCGAGCATTGTGCCTATGCTTGATAAAGAAATTGCTACGC
AGTTTATGAAGCTACAGCAAAAACAAGGTATAGAGTTTAAACTAAATACTAGAGTGTTGTCTGCTGAAGTAAAGTCAGGA
AAAGTAAATCTGACAATAGAGGAGGGCGGTAAGAGTGCGGTAGTAACTAGCGATGTGATATTAATGGCAGTAGGTAGAAA
AGCCTACACGCAAAATCTAGGGCTTGAGTCTGTCGGCATTATTACAGATAAGCAAGGTAGAATTGAGATTAATGACCGGT
TCCAAACAGCAATTTCAAATATTTATGCAGTCGGGGATGTGGTAAAAGGAGCTATGCTTGCTCATAAAGCCGAGGAAGAG
GCTGTAGCTGCCGTGGAAATTATGGCAGGGCAGGCAGGACATGTTAACTATAATCTAATTCCAAGCGTAATATATACTTA
TCCTGAAGTAGCAAGCGTCGGTGAAACGGAAGAACAGTTAAAAGCAAAAGGCATTAATTATAAAGTAGGTAAGTTTCCGT
TTTTAGCAAATAGTAGAGCAAGAGCAATCGGTAGTACGGAAGGGCTTGTTAAGATCCTTGCCGATAGTAAAACCGATAGG
GTTTTAGGAGCTCATATTATAGGAGCCAATGCAGGTACACTCATTGCCGAGCTTACGGCTTATATGGAATTCGGAGCAGC
TTCAGAAGATATAGCAAGAACCTGCCACGCCCACCCGACTTTAAGCGAGGCTATAAAAGAAGCAGCTCTTAGTGTGGATA
AGAGGAGTATCAATATGTGA

Upstream 100 bases:

>100_bases
TCCCGCGCCTTGATTTTGGGATCCAGTCTTTTTTAATTTTTTTCTGGATACCGTGGTCAAGCCACGGTATGACAAAGAGA
GGATAATATAAGGTAAACAT

Downstream 100 bases:

>100_bases
TATTGTTCTACTCCAATGTCATTCCTGCGTAGGAGGGAATCTAGTAAAACATATAAAAAACTTGTTTTAAATGTTTATTT
TCAAATATTGAATGTAATTT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MQEFDLVVIGSGPAGYTGSIRAAQLGMKVVCVEKNDTLGGTCLNIGCIPSKALLNSSEKYEEALKHFESIGITADVKLDL
QKMIANKDKVVSDLTKGIESLFAKNKITRIKGEAKIIASNIVEVNNEQIKAKNILITTGSSVIEIPNIKIDEEFIVSSTG
ALKLSKVPETLIVVGGGYIGLELGSVWRRLGAKVTVIEYAPSIVPMLDKEIATQFMKLQQKQGIEFKLNTRVLSAEVKSG
KVNLTIEEGGKSAVVTSDVILMAVGRKAYTQNLGLESVGIITDKQGRIEINDRFQTAISNIYAVGDVVKGAMLAHKAEEE
AVAAVEIMAGQAGHVNYNLIPSVIYTYPEVASVGETEEQLKAKGINYKVGKFPFLANSRARAIGSTEGLVKILADSKTDR
VLGAHIIGANAGTLIAELTAYMEFGAASEDIARTCHAHPTLSEAIKEAALSVDKRSINM

Sequences:

>Translated_459_residues
MQEFDLVVIGSGPAGYTGSIRAAQLGMKVVCVEKNDTLGGTCLNIGCIPSKALLNSSEKYEEALKHFESIGITADVKLDL
QKMIANKDKVVSDLTKGIESLFAKNKITRIKGEAKIIASNIVEVNNEQIKAKNILITTGSSVIEIPNIKIDEEFIVSSTG
ALKLSKVPETLIVVGGGYIGLELGSVWRRLGAKVTVIEYAPSIVPMLDKEIATQFMKLQQKQGIEFKLNTRVLSAEVKSG
KVNLTIEEGGKSAVVTSDVILMAVGRKAYTQNLGLESVGIITDKQGRIEINDRFQTAISNIYAVGDVVKGAMLAHKAEEE
AVAAVEIMAGQAGHVNYNLIPSVIYTYPEVASVGETEEQLKAKGINYKVGKFPFLANSRARAIGSTEGLVKILADSKTDR
VLGAHIIGANAGTLIAELTAYMEFGAASEDIARTCHAHPTLSEAIKEAALSVDKRSINM
>Mature_459_residues
MQEFDLVVIGSGPAGYTGSIRAAQLGMKVVCVEKNDTLGGTCLNIGCIPSKALLNSSEKYEEALKHFESIGITADVKLDL
QKMIANKDKVVSDLTKGIESLFAKNKITRIKGEAKIIASNIVEVNNEQIKAKNILITTGSSVIEIPNIKIDEEFIVSSTG
ALKLSKVPETLIVVGGGYIGLELGSVWRRLGAKVTVIEYAPSIVPMLDKEIATQFMKLQQKQGIEFKLNTRVLSAEVKSG
KVNLTIEEGGKSAVVTSDVILMAVGRKAYTQNLGLESVGIITDKQGRIEINDRFQTAISNIYAVGDVVKGAMLAHKAEEE
AVAAVEIMAGQAGHVNYNLIPSVIYTYPEVASVGETEEQLKAKGINYKVGKFPFLANSRARAIGSTEGLVKILADSKTDR
VLGAHIIGANAGTLIAELTAYMEFGAASEDIARTCHAHPTLSEAIKEAALSVDKRSINM

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=467, Percent_Identity=56.3169164882227, Blast_Score=512, Evalue=1e-145,
Organism=Homo sapiens, GI50301238, Length=466, Percent_Identity=29.6137339055794, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI33519430, Length=472, Percent_Identity=26.6949152542373, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI33519428, Length=472, Percent_Identity=26.6949152542373, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI33519426, Length=472, Percent_Identity=26.6949152542373, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI148277065, Length=472, Percent_Identity=26.6949152542373, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI148277071, Length=472, Percent_Identity=26.6949152542373, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI291045266, Length=435, Percent_Identity=25.5172413793103, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI22035672, Length=476, Percent_Identity=26.0504201680672, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI291045268, Length=423, Percent_Identity=24.3498817966903, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1786307, Length=451, Percent_Identity=39.689578713969, Blast_Score=324, Evalue=8e-90,
Organism=Escherichia coli, GI87082354, Length=462, Percent_Identity=28.7878787878788, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI87081717, Length=457, Percent_Identity=29.5404814004376, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789915, Length=431, Percent_Identity=29.4663573085847, Blast_Score=179, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI32565766, Length=458, Percent_Identity=52.6200873362445, Blast_Score=491, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI71983419, Length=460, Percent_Identity=26.0869565217391, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=460, Percent_Identity=26.304347826087, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17557007, Length=487, Percent_Identity=26.4887063655031, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=497, Percent_Identity=25.3521126760563, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17559934, Length=239, Percent_Identity=27.1966527196653, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6321091, Length=476, Percent_Identity=50.6302521008403, Blast_Score=451, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6325240, Length=467, Percent_Identity=32.9764453961456, Blast_Score=244, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6325166, Length=467, Percent_Identity=27.6231263383298, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=54.7045951859956, Blast_Score=494, Evalue=1e-140,
Organism=Drosophila melanogaster, GI24640551, Length=489, Percent_Identity=28.4253578732106, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24640553, Length=490, Percent_Identity=28.9795918367347, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24640549, Length=490, Percent_Identity=28.9795918367347, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI17737741, Length=495, Percent_Identity=25.4545454545455, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49121; Mature: 49121

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEFDLVVIGSGPAGYTGSIRAAQLGMKVVCVEKNDTLGGTCLNIGCIPSKALLNSSEKY
CCCEEEEEECCCCCCCCCCCCHHHCCEEEEEEECCCCCCCEEEEECCCCCHHHHCCCHHH
EEALKHFESIGITADVKLDLQKMIANKDKVVSDLTKGIESLFAKNKITRIKGEAKIIASN
HHHHHHHHHCCCEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEH
IVEVNNEQIKAKNILITTGSSVIEIPNIKIDEEFIVSSTGALKLSKVPETLIVVGGGYIG
HEECCCCEEEEEEEEEECCCCEEECCCCEECCHHEEECCCCEEEECCCCEEEEEECCEEE
LELGSVWRRLGAKVTVIEYAPSIVPMLDKEIATQFMKLQQKQGIEFKLNTRVLSAEVKSG
EEHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEEEECCC
KVNLTIEEGGKSAVVTSDVILMAVGRKAYTQNLGLESVGIITDKQGRIEINDRFQTAISN
EEEEEEECCCCEEEEECCEEEEECCCHHHHHCCCCCEEEEEECCCCEEEECHHHHHHHHH
IYAVGDVVKGAMLAHKAEEEAVAAVEIMAGQAGHVNYNLIPSVIYTYPEVASVGETEEQL
HHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEHHHHHHCCCCHHCCCCCHHHH
KAKGINYKVGKFPFLANSRARAIGSTEGLVKILADSKTDRVLGAHIIGANAGTLIAELTA
HHCCCCEECCCCCEECCCCHHHCCCCCCHHEEEECCCCCCEEEEEEECCCCHHHHHHHHH
YMEFGAASEDIARTCHAHPTLSEAIKEAALSVDKRSINM
HHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure
MQEFDLVVIGSGPAGYTGSIRAAQLGMKVVCVEKNDTLGGTCLNIGCIPSKALLNSSEKY
CCCEEEEEECCCCCCCCCCCCHHHCCEEEEEEECCCCCCCEEEEECCCCCHHHHCCCHHH
EEALKHFESIGITADVKLDLQKMIANKDKVVSDLTKGIESLFAKNKITRIKGEAKIIASN
HHHHHHHHHCCCEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEH
IVEVNNEQIKAKNILITTGSSVIEIPNIKIDEEFIVSSTGALKLSKVPETLIVVGGGYIG
HEECCCCEEEEEEEEEECCCCEEECCCCEECCHHEEECCCCEEEECCCCEEEEEECCEEE
LELGSVWRRLGAKVTVIEYAPSIVPMLDKEIATQFMKLQQKQGIEFKLNTRVLSAEVKSG
EEHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEEEECCC
KVNLTIEEGGKSAVVTSDVILMAVGRKAYTQNLGLESVGIITDKQGRIEINDRFQTAISN
EEEEEEECCCCEEEEECCEEEEECCCHHHHHCCCCCEEEEEECCCCEEEECHHHHHHHHH
IYAVGDVVKGAMLAHKAEEEAVAAVEIMAGQAGHVNYNLIPSVIYTYPEVASVGETEEQL
HHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEHHHHHHCCCCHHCCCCCHHHH
KAKGINYKVGKFPFLANSRARAIGSTEGLVKILADSKTDRVLGAHIIGANAGTLIAELTA
HHCCCCEECCCCCEECCCCHHHCCCCCCHHEEEECCCCCCEEEEEEECCCCHHHHHHHHH
YMEFGAASEDIARTCHAHPTLSEAIKEAALSVDKRSINM
HHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]