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The map label for this gene is priA [H]

Identifier: 157825861

GI number: 157825861

Start: 717412

End: 719358

Strand: Reverse

Name: priA [H]

Synonym: A1C_04000

Alternate gene names: 157825861

Gene position: 719358-717412 (Counterclockwise)

Preceding gene: 157825864

Following gene: 157825860

Centisome position: 58.43

GC content: 32.46

Gene sequence:

>1947_bases
ATGCGAATAGCAAAAATATTATTACCGGCAGCAAAATTATTCCCTTTAGATTATTTAATAGCTGAAGATTTAGAGCTAAA
TATCGGTGATCTTGTTGTGGTGCCTTTTAGAAATAAGGAATTAACCGGCATAATATGGGAGTTTACCACTATTCCTGAAG
CAAAAAAGCTAAAAATCATAAAAGAAAAAGTACCGTTAAATTTAAATATTACTTTAGAAGTTTTAGAATTAATAAAATGG
ATGAGTAGCTACTATATGTCGGAACTTGGGAGCATAGCAAAATTAGTATTACCTGTAGATATTTCTGAAAAACCGATTAA
AGTTAAAGAGCAGAAAGTAAATCATAACTTTGTGCTACCTAATTTGTCAGAAGAGCAGAATATGGCAGTAACAGTCTTAA
ATGAAAGCAGTAAGCCTGTGATTATTAAAGGTGTTACGGGGTCAGGTAAAACGGAAATATATTTTCATCTAATAGCAGAC
TACTTAGCAAAAGGAAAACAAGTGCTTGTTATGTTGCCTGAAATTGCTTTAAGTACACAAATTATTAATCGTTTTATAGA
GCGATTCGGTTTTGAGCCTATCATATGGAACTCAAACGTTACAAAAGCTCAAAAGAAAATGATTTTAAGAGGTATATTAA
GCGATAAAGTTAAAGTAGTAATTGGGGCTAGAAGTAGTTTATTTTTACCTTTTAAAAATCTCGGTTTAATAGTTATAGAT
GAGGAGCATGACGATTCCTATAAACAAGATGACGGGATATTGTATAATGCTAGGGATACGGCTATCGTAAGGGGTAGATT
TGATAAAGCACAAATCGTTTTGTGTTCGGCAACACCTTCTATTGAAACGATATATAATATAGAAACAGGTAAATATCAAC
GAATTACATTGGTTAATAGATATAAAGATGTCGATTTGCCAAATATAGAAATAATCGACATGACTAAAGAAAAGCTAGCT
AAGAATTCCTATTTATCCAAGCTTCTTATAAAAGCTATCAAAGATAATTTAGATAATAAAAAGCAAGTATTATTATTTCT
TAATAGGCGTGGTTATGCTCCGCTCATGTTATGCAAAACTTGTGGTCACAGATTTACCTGTAAATTCTGCTCTTCTTGGA
TGGTAGTACATAAATCAACTAAAAAACTTGAATGTCACCATTGCGGTTATCAAAGTAAAATTTTTAGTTCTTGTCCTGAA
TGTCTAGAGGGTGAAACATTAACTATCTGTGGTCCAGGAATAGAAAGGATAGAGGAAGAAGCAAAAGCACTTTTTCCTGA
GAGTAAAATTGCGTTGATAAGTAAAGATCATGCTAAAAATCCGGAAAAAATAGTACAACTTCTACATCAAATGGAAAATC
TAGAAATTGATATATTAATAGGAACGCAAATGATAACGAAAGGCTATCATTTTCCGTATCTTACTTTAGTTGGAGTAATA
GATGCTGATCTTGGTAGTAATAATGCCGATCTTAGAGCATCTGAGCGAACTTTTCAGCTACTGCATCAAGTAGGAGGTAG
AGCTGGTAGGGGAGATAGTAAAGGTGTAGTATATTTACAAAGCTATTATCCTGATAATATAATTTTTAGTTATGTTAAAG
CTGGTGATGAAGATAGTTTTTTCGCGCATGAACTTGAAATAAGAAAATCAGCAGATATGCCGCCCTTTTCTAAAACAGCA
TCGATAATTTTATCAGGTTCTAGTGAATCTAAAATTCTAGAAATAGCACGAGATATGGTACGAATTGCACCGAAAGCAAA
CGTGAAAATTTTAGGACCGGCAAGCTCATTAATGTCAAAGCTCGCCGGTAAATATCGTTACAGGATATTCATTATAGCCA
GTAAGCAATTTAATTTGCAGAAATATTTAAAATTTTGGCTAAGCCTTATAAAAATTCCTTCTTTTTGCCATCTAAAAATA
GATATCGATCCTAAAAGTTTTTATTAG

Upstream 100 bases:

>100_bases
GGAAATTGCTATAATAATCTTTTTATCTCTCATCATCTAAATTTCTACAATCCATTAAATTTAGTATATAATAAAAAAAA
AATTATAAAATACATTATAT

Downstream 100 bases:

>100_bases
CTTCTATATCTTGCTCCTTTACTCTTTGACGGTGGAACATTAGATGAATGAGGAGAAGGATTAGTAAGAGATTCTTGTAT
TTCTGCTATAGCTTTTTTAG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MRIAKILLPAAKLFPLDYLIAEDLELNIGDLVVVPFRNKELTGIIWEFTTIPEAKKLKIIKEKVPLNLNITLEVLELIKW
MSSYYMSELGSIAKLVLPVDISEKPIKVKEQKVNHNFVLPNLSEEQNMAVTVLNESSKPVIIKGVTGSGKTEIYFHLIAD
YLAKGKQVLVMLPEIALSTQIINRFIERFGFEPIIWNSNVTKAQKKMILRGILSDKVKVVIGARSSLFLPFKNLGLIVID
EEHDDSYKQDDGILYNARDTAIVRGRFDKAQIVLCSATPSIETIYNIETGKYQRITLVNRYKDVDLPNIEIIDMTKEKLA
KNSYLSKLLIKAIKDNLDNKKQVLLFLNRRGYAPLMLCKTCGHRFTCKFCSSWMVVHKSTKKLECHHCGYQSKIFSSCPE
CLEGETLTICGPGIERIEEEAKALFPESKIALISKDHAKNPEKIVQLLHQMENLEIDILIGTQMITKGYHFPYLTLVGVI
DADLGSNNADLRASERTFQLLHQVGGRAGRGDSKGVVYLQSYYPDNIIFSYVKAGDEDSFFAHELEIRKSADMPPFSKTA
SIILSGSSESKILEIARDMVRIAPKANVKILGPASSLMSKLAGKYRYRIFIIASKQFNLQKYLKFWLSLIKIPSFCHLKI
DIDPKSFY

Sequences:

>Translated_648_residues
MRIAKILLPAAKLFPLDYLIAEDLELNIGDLVVVPFRNKELTGIIWEFTTIPEAKKLKIIKEKVPLNLNITLEVLELIKW
MSSYYMSELGSIAKLVLPVDISEKPIKVKEQKVNHNFVLPNLSEEQNMAVTVLNESSKPVIIKGVTGSGKTEIYFHLIAD
YLAKGKQVLVMLPEIALSTQIINRFIERFGFEPIIWNSNVTKAQKKMILRGILSDKVKVVIGARSSLFLPFKNLGLIVID
EEHDDSYKQDDGILYNARDTAIVRGRFDKAQIVLCSATPSIETIYNIETGKYQRITLVNRYKDVDLPNIEIIDMTKEKLA
KNSYLSKLLIKAIKDNLDNKKQVLLFLNRRGYAPLMLCKTCGHRFTCKFCSSWMVVHKSTKKLECHHCGYQSKIFSSCPE
CLEGETLTICGPGIERIEEEAKALFPESKIALISKDHAKNPEKIVQLLHQMENLEIDILIGTQMITKGYHFPYLTLVGVI
DADLGSNNADLRASERTFQLLHQVGGRAGRGDSKGVVYLQSYYPDNIIFSYVKAGDEDSFFAHELEIRKSADMPPFSKTA
SIILSGSSESKILEIARDMVRIAPKANVKILGPASSLMSKLAGKYRYRIFIIASKQFNLQKYLKFWLSLIKIPSFCHLKI
DIDPKSFY
>Mature_648_residues
MRIAKILLPAAKLFPLDYLIAEDLELNIGDLVVVPFRNKELTGIIWEFTTIPEAKKLKIIKEKVPLNLNITLEVLELIKW
MSSYYMSELGSIAKLVLPVDISEKPIKVKEQKVNHNFVLPNLSEEQNMAVTVLNESSKPVIIKGVTGSGKTEIYFHLIAD
YLAKGKQVLVMLPEIALSTQIINRFIERFGFEPIIWNSNVTKAQKKMILRGILSDKVKVVIGARSSLFLPFKNLGLIVID
EEHDDSYKQDDGILYNARDTAIVRGRFDKAQIVLCSATPSIETIYNIETGKYQRITLVNRYKDVDLPNIEIIDMTKEKLA
KNSYLSKLLIKAIKDNLDNKKQVLLFLNRRGYAPLMLCKTCGHRFTCKFCSSWMVVHKSTKKLECHHCGYQSKIFSSCPE
CLEGETLTICGPGIERIEEEAKALFPESKIALISKDHAKNPEKIVQLLHQMENLEIDILIGTQMITKGYHFPYLTLVGVI
DADLGSNNADLRASERTFQLLHQVGGRAGRGDSKGVVYLQSYYPDNIIFSYVKAGDEDSFFAHELEIRKSADMPPFSKTA
SIILSGSSESKILEIARDMVRIAPKANVKILGPASSLMSKLAGKYRYRIFIIASKQFNLQKYLKFWLSLIKIPSFCHLKI
DIDPKSFY

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=538, Percent_Identity=36.4312267657993, Blast_Score=352, Evalue=3e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 73366; Mature: 73366

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAKILLPAAKLFPLDYLIAEDLELNIGDLVVVPFRNKELTGIIWEFTTIPEAKKLKII
CCHHHHHHCHHHHCCHHHHHHCCCEECCCCEEEEEECCCCEEEEEEEEECCCCHHHHHHH
KEKVPLNLNITLEVLELIKWMSSYYMSELGSIAKLVLPVDISEKPIKVKEQKVNHNFVLP
HHHCCEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEHEECCCCEECC
NLSEEQNMAVTVLNESSKPVIIKGVTGSGKTEIYFHLIADYLAKGKQVLVMLPEIALSTQ
CCCCCCCEEEEEEECCCCCEEEEECCCCCCCEEHHHHHHHHHCCCCEEEEEECHHHHHHH
IINRFIERFGFEPIIWNSNVTKAQKKMILRGILSDKVKVVIGARSSLFLPFKNLGLIVID
HHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEHHHCCEEEEE
EEHDDSYKQDDGILYNARDTAIVRGRFDKAQIVLCSATPSIETIYNIETGKYQRITLVNR
CCCCCCCCCCCCEEEECCCCEEEEECCCCEEEEEEECCCCCEEEEEECCCCEEEEEEEEC
YKDVDLPNIEIIDMTKEKLAKNSYLSKLLIKAIKDNLDNKKQVLLFLNRRGYAPLMLCKT
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHH
CGHRFTCKFCSSWMVVHKSTKKLECHHCGYQSKIFSSCPECLEGETLTICGPGIERIEEE
CCCCEEEHHHCCEEEEECCCCCEEHHHCCCHHHHHHCCHHHHCCCEEEEECCCHHHHHHH
AKALFPESKIALISKDHAKNPEKIVQLLHQMENLEIDILIGTQMITKGYHFPYLTLVGVI
HHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHCCCCCCHHHEEEHH
DADLGSNNADLRASERTFQLLHQVGGRAGRGDSKGVVYLQSYYPDNIIFSYVKAGDEDSF
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHEEHHHHCCCCCCE
FAHELEIRKSADMPPFSKTASIILSGSSESKILEIARDMVRIAPKANVKILGPASSLMSK
EEEEEEHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHH
LAGKYRYRIFIIASKQFNLQKYLKFWLSLIKIPSFCHLKIDIDPKSFY
HCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MRIAKILLPAAKLFPLDYLIAEDLELNIGDLVVVPFRNKELTGIIWEFTTIPEAKKLKII
CCHHHHHHCHHHHCCHHHHHHCCCEECCCCEEEEEECCCCEEEEEEEEECCCCHHHHHHH
KEKVPLNLNITLEVLELIKWMSSYYMSELGSIAKLVLPVDISEKPIKVKEQKVNHNFVLP
HHHCCEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEHEECCCCEECC
NLSEEQNMAVTVLNESSKPVIIKGVTGSGKTEIYFHLIADYLAKGKQVLVMLPEIALSTQ
CCCCCCCEEEEEEECCCCCEEEEECCCCCCCEEHHHHHHHHHCCCCEEEEEECHHHHHHH
IINRFIERFGFEPIIWNSNVTKAQKKMILRGILSDKVKVVIGARSSLFLPFKNLGLIVID
HHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEHHHCCEEEEE
EEHDDSYKQDDGILYNARDTAIVRGRFDKAQIVLCSATPSIETIYNIETGKYQRITLVNR
CCCCCCCCCCCCEEEECCCCEEEEECCCCEEEEEEECCCCCEEEEEECCCCEEEEEEEEC
YKDVDLPNIEIIDMTKEKLAKNSYLSKLLIKAIKDNLDNKKQVLLFLNRRGYAPLMLCKT
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHH
CGHRFTCKFCSSWMVVHKSTKKLECHHCGYQSKIFSSCPECLEGETLTICGPGIERIEEE
CCCCEEEHHHCCEEEEECCCCCEEHHHCCCHHHHHHCCHHHHCCCEEEEECCCHHHHHHH
AKALFPESKIALISKDHAKNPEKIVQLLHQMENLEIDILIGTQMITKGYHFPYLTLVGVI
HHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHCCCCCCHHHEEEHH
DADLGSNNADLRASERTFQLLHQVGGRAGRGDSKGVVYLQSYYPDNIIFSYVKAGDEDSF
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHEEHHHHCCCCCCE
FAHELEIRKSADMPPFSKTASIILSGSSESKILEIARDMVRIAPKANVKILGPASSLMSK
EEEEEEHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHH
LAGKYRYRIFIIASKQFNLQKYLKFWLSLIKIPSFCHLKIDIDPKSFY
HCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]