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The map label for this gene is puuA [H]

Identifier: 157825790

GI number: 157825790

Start: 652487

End: 653320

Strand: Reverse

Name: puuA [H]

Synonym: A1C_03605

Alternate gene names: 157825790

Gene position: 653320-652487 (Counterclockwise)

Preceding gene: 157825794

Following gene: 157825788

Centisome position: 53.07

GC content: 29.14

Gene sequence:

>834_bases
ATGAAACTACCCTATATTAATTATAATAGTTTTATTAATACTTTATCCAAACGTTTACAAAAAAATAAACAGTTGGAAAA
AATAATAGCACAATTTCACCAAGACTATAACCTAATTCCTATTATAGGCGTAGAAATAGAGTTTTATTTAAGTCACAATG
TTGATATAGCTAAATTTGAAATACTTTCAAGCAAATACTTAGCTAGATTCAAAATTGCTAAAATAAAAAAAGAAAAAGGC
AATAACCAATTTGAAATAGATCTCCCTCCTTCTGTAAACTTAATACAATATATCCACAATATATTAGAGGTTAAAAATAT
TTTAAAAAAAATTGCACAGCAGTTAAACGGGTATATAGATTTTTCTCCTAAGCCATTTTTGGATGATTATGGTAATAGTA
TGCATTTTCATATTAATTTTAATTCTGCGTTCAATGATTATTATATAATTTTAGCAGCACAAGGGCTATGTCACTATATG
CTAGATACCATACTCGCTTTTATGCCTACTACTCTAGATTATTCACGAATAAATAAACAATTTATGGCTCCTACTCATAT
ATCGTACGGTGGAAATAATAGAAGTGTAGCCGTACGAACTCCTAATTCTTTTCCTAAACGTTTAGAGCATCGCTTAGCTT
CTCCCGAAACCGATCCATATATAGCAATTTTTACTATTTTAAAGTCAATATTGTTGGCTTTAAGATCTCCAAGTTCGCTT
CACAAAATAGAAAAAATTTACGGGAATGCTTTCGATCCGCAATATAATTTAACTCCATTACCTACGTCATCTCAAGAGAG
CTTTATGTTATTTAAACCGGACTTTTTTAAATAA

Upstream 100 bases:

>100_bases
TTCATTTATAGAGTTATTTTTTTTTATATATCTATGACTAGAATTATTGAATGTTTTATCAAAAATTTTATAATAAACTG
ATTCGTTGATTAATCCTTCC

Downstream 100 bases:

>100_bases
ATTGTACTTCTAAAATAAAGATTGATATAAAGTTTTAAATATAAAACTGCCGAAATTGTTTCAATTAATTATTTTTTTTG
TAAAATTAGATAATTATGAA

Product: glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKLPYINYNSFINTLSKRLQKNKQLEKIIAQFHQDYNLIPIIGVEIEFYLSHNVDIAKFEILSSKYLARFKIAKIKKEKG
NNQFEIDLPPSVNLIQYIHNILEVKNILKKIAQQLNGYIDFSPKPFLDDYGNSMHFHINFNSAFNDYYIILAAQGLCHYM
LDTILAFMPTTLDYSRINKQFMAPTHISYGGNNRSVAVRTPNSFPKRLEHRLASPETDPYIAIFTILKSILLALRSPSSL
HKIEKIYGNAFDPQYNLTPLPTSSQESFMLFKPDFFK

Sequences:

>Translated_277_residues
MKLPYINYNSFINTLSKRLQKNKQLEKIIAQFHQDYNLIPIIGVEIEFYLSHNVDIAKFEILSSKYLARFKIAKIKKEKG
NNQFEIDLPPSVNLIQYIHNILEVKNILKKIAQQLNGYIDFSPKPFLDDYGNSMHFHINFNSAFNDYYIILAAQGLCHYM
LDTILAFMPTTLDYSRINKQFMAPTHISYGGNNRSVAVRTPNSFPKRLEHRLASPETDPYIAIFTILKSILLALRSPSSL
HKIEKIYGNAFDPQYNLTPLPTSSQESFMLFKPDFFK
>Mature_277_residues
MKLPYINYNSFINTLSKRLQKNKQLEKIIAQFHQDYNLIPIIGVEIEFYLSHNVDIAKFEILSSKYLARFKIAKIKKEKG
NNQFEIDLPPSVNLIQYIHNILEVKNILKKIAQQLNGYIDFSPKPFLDDYGNSMHFHINFNSAFNDYYIILAAQGLCHYM
LDTILAFMPTTLDYSRINKQFMAPTHISYGGNNRSVAVRTPNSFPKRLEHRLASPETDPYIAIFTILKSILLALRSPSSL
HKIEKIYGNAFDPQYNLTPLPTSSQESFMLFKPDFFK

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87081870, Length=294, Percent_Identity=24.8299319727891, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790301, Length=164, Percent_Identity=28.0487804878049, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 32142; Mature: 32142

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPYINYNSFINTLSKRLQKNKQLEKIIAQFHQDYNLIPIIGVEIEFYLSHNVDIAKFE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCHHHHH
ILSSKYLARFKIAKIKKEKGNNQFEIDLPPSVNLIQYIHNILEVKNILKKIAQQLNGYID
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEC
FSPKPFLDDYGNSMHFHINFNSAFNDYYIILAAQGLCHYMLDTILAFMPTTLDYSRINKQ
CCCCCCHHHCCCEEEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FMAPTHISYGGNNRSVAVRTPNSFPKRLEHRLASPETDPYIAIFTILKSILLALRSPSSL
HCCCCEEEECCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCHHH
HKIEKIYGNAFDPQYNLTPLPTSSQESFMLFKPDFFK
HHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCC
>Mature Secondary Structure
MKLPYINYNSFINTLSKRLQKNKQLEKIIAQFHQDYNLIPIIGVEIEFYLSHNVDIAKFE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCHHHHH
ILSSKYLARFKIAKIKKEKGNNQFEIDLPPSVNLIQYIHNILEVKNILKKIAQQLNGYID
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEC
FSPKPFLDDYGNSMHFHINFNSAFNDYYIILAAQGLCHYMLDTILAFMPTTLDYSRINKQ
CCCCCCHHHCCCEEEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FMAPTHISYGGNNRSVAVRTPNSFPKRLEHRLASPETDPYIAIFTILKSILLALRSPSSL
HCCCCEEEECCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCHHH
HKIEKIYGNAFDPQYNLTPLPTSSQESFMLFKPDFFK
HHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]