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The map label for this gene is xthA [H]

Identifier: 157825475

GI number: 157825475

Start: 352631

End: 353464

Strand: Direct

Name: xthA [H]

Synonym: A1C_01880

Alternate gene names: 157825475

Gene position: 352631-353464 (Clockwise)

Preceding gene: 157825474

Following gene: 157825476

Centisome position: 28.64

GC content: 34.17

Gene sequence:

>834_bases
ATGAAGATAGTGACTTGGAATATTAATTCATTGCGTCTACGGATTGACTTATTAAGAAAATTAGTATATGAACATCAGCC
GGATATTATTTTACTTCAAGAAACCAAGGTATCGGACTCATTATTTCCTCTTGAAGTTATTAAAAATATAGGCTATGAAC
ATGTAATATATTCAGGGCAGAAGTCATATAATGGTGTTGCTATTATTTCAAAATTTCCTTTGAATAATGTTTTTTCATTA
GAGTTATATAATAGTGATAAAAGGCATATAGCGGCTATAGTTAATGATATAGAAATACATAATTTTTATGTTCCTGCTGG
TGGTGATATACCTGATATAGATGCAAACTTAAAATTTAAGCATAAGCTCGAATATGTAAGGTTAATGCAGGAATGGCTAA
CTACTAACCGTACTAGGAACGATAAAATTATCATTGTTGGGGACTTGAATATTGCTCCGCATGAACATGATGTATGGTCT
AGTAAGCAGTTGAGAAATGTTATCAGCCATACCGATATTGAACGCTCGTTATTGGTAGAGCTACAAAATTCGTTAAGTTT
TATCGATAGTAGCAGGTATTTTGTTCCACTCGACGAAAAGTTTTATACTTGGTGGAGCTATAGAAATATAGATTGGAAAA
AATCTAACAGAGGTAGAAGGCTTGATCATATTTGGGTTAGCAATAATTTAAAAGATGCATTATTTTCTATGCATTTACTA
TCAGAAGCACGTGATTGGTTGCAGCCTTCAGACCATGTGCCGTATTTTGTGACTTTGGATGTCATTCCCGTGGAGGCGGG
CATCCAAAAACAGATGTCATCCAGTGGCTCTTGA

Upstream 100 bases:

>100_bases
AAAATTGACATGAAAAATCCTTTAAATCAACTTGGAATAATTTATTTCAGAGATGCAATAATAGAGGCAGCTATAAAAAA
TGATTAAATAAAAACATTAA

Downstream 100 bases:

>100_bases
CCACTGGATGAAAATATACTATAGGAGTATAGGAGTAATGAGTGGATATTAAGTTAGGAAAATATAAGCCGATCAAAGAG
GAATATTTAAGAGCTTTTAA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKIVTWNINSLRLRIDLLRKLVYEHQPDIILLQETKVSDSLFPLEVIKNIGYEHVIYSGQKSYNGVAIISKFPLNNVFSL
ELYNSDKRHIAAIVNDIEIHNFYVPAGGDIPDIDANLKFKHKLEYVRLMQEWLTTNRTRNDKIIIVGDLNIAPHEHDVWS
SKQLRNVISHTDIERSLLVELQNSLSFIDSSRYFVPLDEKFYTWWSYRNIDWKKSNRGRRLDHIWVSNNLKDALFSMHLL
SEARDWLQPSDHVPYFVTLDVIPVEAGIQKQMSSSGS

Sequences:

>Translated_277_residues
MKIVTWNINSLRLRIDLLRKLVYEHQPDIILLQETKVSDSLFPLEVIKNIGYEHVIYSGQKSYNGVAIISKFPLNNVFSL
ELYNSDKRHIAAIVNDIEIHNFYVPAGGDIPDIDANLKFKHKLEYVRLMQEWLTTNRTRNDKIIIVGDLNIAPHEHDVWS
SKQLRNVISHTDIERSLLVELQNSLSFIDSSRYFVPLDEKFYTWWSYRNIDWKKSNRGRRLDHIWVSNNLKDALFSMHLL
SEARDWLQPSDHVPYFVTLDVIPVEAGIQKQMSSSGS
>Mature_277_residues
MKIVTWNINSLRLRIDLLRKLVYEHQPDIILLQETKVSDSLFPLEVIKNIGYEHVIYSGQKSYNGVAIISKFPLNNVFSL
ELYNSDKRHIAAIVNDIEIHNFYVPAGGDIPDIDANLKFKHKLEYVRLMQEWLTTNRTRNDKIIIVGDLNIAPHEHDVWS
SKQLRNVISHTDIERSLLVELQNSLSFIDSSRYFVPLDEKFYTWWSYRNIDWKKSNRGRRLDHIWVSNNLKDALFSMHLL
SEARDWLQPSDHVPYFVTLDVIPVEAGIQKQMSSSGS

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=262, Percent_Identity=29.0076335877863, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI18375503, Length=262, Percent_Identity=29.0076335877863, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI18375501, Length=262, Percent_Identity=29.0076335877863, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1788046, Length=272, Percent_Identity=31.9852941176471, Blast_Score=136, Evalue=2e-33,
Organism=Drosophila melanogaster, GI221330655, Length=267, Percent_Identity=28.0898876404494, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17136678, Length=267, Percent_Identity=28.0898876404494, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 32390; Mature: 32390

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVTWNINSLRLRIDLLRKLVYEHQPDIILLQETKVSDSLFPLEVIKNIGYEHVIYSGQ
CEEEEEECCCEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCEEEECCC
KSYNGVAIISKFPLNNVFSLELYNSDKRHIAAIVNDIEIHNFYVPAGGDIPDIDANLKFK
CCCCCEEEEEECCCCCEEEEEEECCCCCEEEEHHEEEEEEEEEECCCCCCCCCCCCCCHH
HKLEYVRLMQEWLTTNRTRNDKIIIVGDLNIAPHEHDVWSSKQLRNVISHTDIERSLLVE
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
LQNSLSFIDSSRYFVPLDEKFYTWWSYRNIDWKKSNRGRRLDHIWVSNNLKDALFSMHLL
HHHHHHHHCCCCEEEEECCCEEEEEEECCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHH
SEARDWLQPSDHVPYFVTLDVIPVEAGIQKQMSSSGS
HHHHHHCCCCCCCCEEEEEEEEECCHHHHHHHHCCCC
>Mature Secondary Structure
MKIVTWNINSLRLRIDLLRKLVYEHQPDIILLQETKVSDSLFPLEVIKNIGYEHVIYSGQ
CEEEEEECCCEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCEEEECCC
KSYNGVAIISKFPLNNVFSLELYNSDKRHIAAIVNDIEIHNFYVPAGGDIPDIDANLKFK
CCCCCEEEEEECCCCCEEEEEEECCCCCEEEEHHEEEEEEEEEECCCCCCCCCCCCCCHH
HKLEYVRLMQEWLTTNRTRNDKIIIVGDLNIAPHEHDVWSSKQLRNVISHTDIERSLLVE
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
LQNSLSFIDSSRYFVPLDEKFYTWWSYRNIDWKKSNRGRRLDHIWVSNNLKDALFSMHLL
HHHHHHHHCCCCEEEEECCCEEEEEEECCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHH
SEARDWLQPSDHVPYFVTLDVIPVEAGIQKQMSSSGS
HHHHHHCCCCCCCCEEEEEEEEECCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]