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The map label for this gene is ddl

Identifier: 157825461

GI number: 157825461

Start: 338040

End: 339002

Strand: Direct

Name: ddl

Synonym: A1C_01810

Alternate gene names: 157825461

Gene position: 338040-339002 (Clockwise)

Preceding gene: 157825460

Following gene: 157825462

Centisome position: 27.46

GC content: 33.44

Gene sequence:

>963_bases
ATGCAATATCAAACACATTGGATAGAACATTCTGTAGTCAAGATATTAAGTACTACCGGTAAAAACCATATAGCGTTAGT
AGCTGGCGGTATGTCTGCTGAGCGTGAAGTATCATTAGTATCAAGCGAAGGGGTTCAGCAAGCCTTAATTGCATTAGGAT
ATAAAGTTACCTTTATTGATATGGGAGCGGATATTGCTGTTAAATTACAAGAAATAAAACCTGATATTGTTTTTAACTGT
TTACACGGTACATATGGTGAAGATGGTTGCTTGCCTGGATTACTTAATATAATGAGGATTCCTTATACTCATAGCGGCGT
GTTATCTTCAGCTTTAGCATTTAATAAAATACATTCTAGCAGTTGGTTTTTCGCGAATAGTATTAATACGGCAGAAAGTA
TTGTTGTAAGTAAAAGCTATAATATAAACACTGATCCTATGAAACGACCATATGTAATCAAACCTCTTACGCAAGGATCA
AGTATTGGTGTTGAAGTTATATTTGAAGAAGATGATTTTAACTTTGCCGATTATGATTTTCCTTACGGTGATCAGGTAGT
AATAGAGCGATATATAAAAGGACGCGAATTTCAAGTAGCGGTATTAAACAGTAAAGCTTTAGGAGCTTTAGAGATTAAAT
TAATAAAAAATCGTTTCTATGATTATGAAACTAAATATACCGAAGGATTTGCTGAACATCTGTGTCCTGCTCCGCTACAT
GCTAATTTATATGAAAAATTACTTATAGAATCAGAAAAAATTTATAAAACAATGAATTGTAAAGGTCCTGCTAGAGCAGA
GTTTATTTTAGAAGAGCAGACAAACAAATTATATGCTTTGGAGATAAATACTCACCCAGGTATGACTCCTTTATCAATAG
TGCCGGAAATTGCAGCTTATGCAGGGATAAATTTTACTAACTTAATTGCAGAAATTATAAAGACGGCAAGCTTTGAATCA
TGA

Upstream 100 bases:

>100_bases
CTATCGCATGTTTTACTGGATTGCTTCGTTAAAACTTATAGTTTTTCTCGCAATGAAAAAATAAATCACAGGATGAAAAT
ATAGAATAAAAGCAAATAAT

Downstream 100 bases:

>100_bases
GAAAAAAAACGAGCTCCAATAAAAAAAACAAAAAAAAAAAACTAATAATATCTCATTACGCCGAAAATTAGGACTAATTT
ACAAAAAAGCAATATTAGGG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MQYQTHWIEHSVVKILSTTGKNHIALVAGGMSAEREVSLVSSEGVQQALIALGYKVTFIDMGADIAVKLQEIKPDIVFNC
LHGTYGEDGCLPGLLNIMRIPYTHSGVLSSALAFNKIHSSSWFFANSINTAESIVVSKSYNINTDPMKRPYVIKPLTQGS
SIGVEVIFEEDDFNFADYDFPYGDQVVIERYIKGREFQVAVLNSKALGALEIKLIKNRFYDYETKYTEGFAEHLCPAPLH
ANLYEKLLIESEKIYKTMNCKGPARAEFILEEQTNKLYALEINTHPGMTPLSIVPEIAAYAGINFTNLIAEIIKTASFES

Sequences:

>Translated_320_residues
MQYQTHWIEHSVVKILSTTGKNHIALVAGGMSAEREVSLVSSEGVQQALIALGYKVTFIDMGADIAVKLQEIKPDIVFNC
LHGTYGEDGCLPGLLNIMRIPYTHSGVLSSALAFNKIHSSSWFFANSINTAESIVVSKSYNINTDPMKRPYVIKPLTQGS
SIGVEVIFEEDDFNFADYDFPYGDQVVIERYIKGREFQVAVLNSKALGALEIKLIKNRFYDYETKYTEGFAEHLCPAPLH
ANLYEKLLIESEKIYKTMNCKGPARAEFILEEQTNKLYALEINTHPGMTPLSIVPEIAAYAGINFTNLIAEIIKTASFES
>Mature_320_residues
MQYQTHWIEHSVVKILSTTGKNHIALVAGGMSAEREVSLVSSEGVQQALIALGYKVTFIDMGADIAVKLQEIKPDIVFNC
LHGTYGEDGCLPGLLNIMRIPYTHSGVLSSALAFNKIHSSSWFFANSINTAESIVVSKSYNINTDPMKRPYVIKPLTQGS
SIGVEVIFEEDDFNFADYDFPYGDQVVIERYIKGREFQVAVLNSKALGALEIKLIKNRFYDYETKYTEGFAEHLCPAPLH
ANLYEKLLIESEKIYKTMNCKGPARAEFILEEQTNKLYALEINTHPGMTPLSIVPEIAAYAGINFTNLIAEIIKTASFES

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=305, Percent_Identity=33.7704918032787, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1786579, Length=350, Percent_Identity=30.2857142857143, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_RICAH (A8GMP8)

Other databases:

- EMBL:   CP000847
- RefSeq:   YP_001493181.1
- ProteinModelPortal:   A8GMP8
- SMR:   A8GMP8
- STRING:   A8GMP8
- GeneID:   5645119
- GenomeReviews:   CP000847_GR
- KEGG:   rak:A1C_01810
- NMPDR:   fig|293614.3.peg.339
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   PLSIVPE
- ProtClustDB:   PRK01372
- BioCyc:   RAKA293614:A1C_01810-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 35666; Mature: 35666

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYQTHWIEHSVVKILSTTGKNHIALVAGGMSAEREVSLVSSEGVQQALIALGYKVTFID
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEHHHCCHHHHHHHHCCEEEEEE
MGADIAVKLQEIKPDIVFNCLHGTYGEDGCLPGLLNIMRIPYTHSGVLSSALAFNKIHSS
CCCCEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
SWFFANSINTAESIVVSKSYNINTDPMKRPYVIKPLTQGSSIGVEVIFEEDDFNFADYDF
CEEEECCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCC
PYGDQVVIERYIKGREFQVAVLNSKALGALEIKLIKNRFYDYETKYTEGFAEHLCPAPLH
CCCCHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEHHHCCCCCCHHHHHHHHHCCCCCCH
ANLYEKLLIESEKIYKTMNCKGPARAEFILEEQTNKLYALEINTHPGMTPLSIVPEIAAY
HHHHHHHHHCHHHHHHHCCCCCCCHHEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHH
AGINFTNLIAEIIKTASFES
CCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQYQTHWIEHSVVKILSTTGKNHIALVAGGMSAEREVSLVSSEGVQQALIALGYKVTFID
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEHHHCCHHHHHHHHCCEEEEEE
MGADIAVKLQEIKPDIVFNCLHGTYGEDGCLPGLLNIMRIPYTHSGVLSSALAFNKIHSS
CCCCEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
SWFFANSINTAESIVVSKSYNINTDPMKRPYVIKPLTQGSSIGVEVIFEEDDFNFADYDF
CEEEECCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCC
PYGDQVVIERYIKGREFQVAVLNSKALGALEIKLIKNRFYDYETKYTEGFAEHLCPAPLH
CCCCHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEHHHCCCCCCHHHHHHHHHCCCCCCH
ANLYEKLLIESEKIYKTMNCKGPARAEFILEEQTNKLYALEINTHPGMTPLSIVPEIAAY
HHHHHHHHHCHHHHHHHCCCCCCCHHEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHH
AGINFTNLIAEIIKTASFES
CCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA