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The map label for this gene is hup [H]

Identifier: 157825356

GI number: 157825356

Start: 228997

End: 229329

Strand: Direct

Name: hup [H]

Synonym: A1C_01220

Alternate gene names: 157825356

Gene position: 228997-229329 (Clockwise)

Preceding gene: 157825354

Following gene: 157825357

Centisome position: 18.6

GC content: 31.83

Gene sequence:

>333_bases
ATGAGTACACATAACAAAAGCGAACCAAAAAAAATGAATAAAACCGAATTTATAGCATTTATGACAGACCATGCTCATAA
TCATAAACATGCTTCACATAAAACATTAACAAAAGCTGATGCTGAGAAAGCACTTAATTTAGTTCTTGATAGTGTGATTA
GTGCTATAAGGAGCCACTATAATATTAATATAACAGGTTTTGGTTCATTTGAAATACATCATAGAAAAGCACGTGAGGGC
CGTAACCCAAAAACTGGAGCAAAAATGAAAATAGATGCTTACAATCAACCTACTTTTAGAGCTGGAAGAAAAATGAAAGA
AGCTTGTAATTAA

Upstream 100 bases:

>100_bases
CATAAAAAATATATTGATGTATATTATTGCCAGTTGTATATATTGTTTTATAAAAAAACATCAATACATATTAACCCATT
AAAGGAGAGTTGATTTTATT

Downstream 100 bases:

>100_bases
AAAAGTAACTTATTACAGTACTGATAGTTTCCAAACCACGTTATAAGTTTGCAGGGTGATACTAGAAAAACCGGCTCATG
CAACAATGCTCTGCAAAAGC

Product: DNA-binding protein HU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 110; Mature: 109

Protein sequence:

>110_residues
MSTHNKSEPKKMNKTEFIAFMTDHAHNHKHASHKTLTKADAEKALNLVLDSVISAIRSHYNINITGFGSFEIHHRKAREG
RNPKTGAKMKIDAYNQPTFRAGRKMKEACN

Sequences:

>Translated_110_residues
MSTHNKSEPKKMNKTEFIAFMTDHAHNHKHASHKTLTKADAEKALNLVLDSVISAIRSHYNINITGFGSFEIHHRKAREG
RNPKTGAKMKIDAYNQPTFRAGRKMKEACN
>Mature_109_residues
STHNKSEPKKMNKTEFIAFMTDHAHNHKHASHKTLTKADAEKALNLVLDSVISAIRSHYNINITGFGSFEIHHRKAREGR
NPKTGAKMKIDAYNQPTFRAGRKMKEACN

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=99, Percent_Identity=36.3636363636364, Blast_Score=77, Evalue=3e-16,
Organism=Escherichia coli, GI1790433, Length=98, Percent_Identity=34.6938775510204, Blast_Score=72, Evalue=7e-15,
Organism=Escherichia coli, GI1787141, Length=99, Percent_Identity=34.3434343434343, Blast_Score=62, Evalue=1e-11,
Organism=Escherichia coli, GI1788005, Length=71, Percent_Identity=36.6197183098592, Blast_Score=59, Evalue=5e-11,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 12486; Mature: 12355

Theoretical pI: Translated: 10.71; Mature: 10.71

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTHNKSEPKKMNKTEFIAFMTDHAHNHKHASHKTLTKADAEKALNLVLDSVISAIRSHY
CCCCCCCCCCCCCCHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NINITGFGSFEIHHRKAREGRNPKTGAKMKIDAYNQPTFRAGRKMKEACN
CCEEEECCCEEEHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
STHNKSEPKKMNKTEFIAFMTDHAHNHKHASHKTLTKADAEKALNLVLDSVISAIRSHY
CCCCCCCCCCCCCHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NINITGFGSFEIHHRKAREGRNPKTGAKMKIDAYNQPTFRAGRKMKEACN
CCEEEECCCEEEHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]