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The map label for this gene is rsmE [H]

Identifier: 157825352

GI number: 157825352

Start: 224328

End: 225092

Strand: Direct

Name: rsmE [H]

Synonym: A1C_01170

Alternate gene names: 157825352

Gene position: 224328-225092 (Clockwise)

Preceding gene: 157825349

Following gene: 157825354

Centisome position: 18.22

GC content: 32.03

Gene sequence:

>765_bases
ATGAGATTTAATCGTATTTATATTAATAGTCGTTTAGCTGAAAATAGCAAGATAGAATTAGCAAGTGATCATGTTCATTA
TGTTAAAACGGTGCTACGCCTTAAAGTAAATGATGGTTTGCGTATCTTTAACGGTACGGAAGGAGAATTTTTAGCACACA
TTACCGCTATAGGTAAGCATAACATATCGGTTAGGCTTAAAGAACAGTTAAAAAAGCCTTATACAGAATCTGCATTAACT
CTTGCTGTTGCTATAATAAAGCAAGATAAATTAATGCTTGCAATAAATATGGCTACGCAACTCGGTATAACTAAAATTAT
TCCGTTAATTACTAGTCGGTGTCAATTTAGAACAGTAAATATAGAACGTTTAACGAAATGTGTTATTGAGGCTACAGAGC
AATCAGAACGTCTTATTCCCCCGATAATTGAAAAAGCTATCACAATACAAGATTGTCTTAAAAAGCATAATAATTTTATG
TTATATGCTAATGAACATGAAAAAGAAGAAAATTCTATATTACGAATTTCATCATTACTGGATAATAATATAGCTATTAT
TATTGGACCGGAGGGAGGTTTTACTAATGATGAACTGGAACTTCTTGCTTCATACAAGAATACAAAATCCGTCAGTTTAG
GAAGTAATATATTACGTGCTGAAACAGCAGTAATAACTGCTATAGTACAAGTGAAGTTGTTAGGTCGTAATTGCGAGGAG
CAAAGCGACGTGGTAATCCAGAAAAATAATAAAAAAATGCTATAA

Upstream 100 bases:

>100_bases
ATTTACTCTTAAATTCATCCTGTCTGAAGCTTTCTGAATTTAGCTGTAAATTGTCTTCTTCTGATATATTATTATATTTT
TCATTCGTCATGGTAATAAT

Downstream 100 bases:

>100_bases
ATTAGCATTTTTTACTGGATTGCTTCGTCGAATTACTGTGTAATTCTTCTCGTAATGACGAAAAGGTAGAGTTACTTCGC
TTTCTCTATTACGGGTTTAC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MRFNRIYINSRLAENSKIELASDHVHYVKTVLRLKVNDGLRIFNGTEGEFLAHITAIGKHNISVRLKEQLKKPYTESALT
LAVAIIKQDKLMLAINMATQLGITKIIPLITSRCQFRTVNIERLTKCVIEATEQSERLIPPIIEKAITIQDCLKKHNNFM
LYANEHEKEENSILRISSLLDNNIAIIIGPEGGFTNDELELLASYKNTKSVSLGSNILRAETAVITAIVQVKLLGRNCEE
QSDVVIQKNNKKML

Sequences:

>Translated_254_residues
MRFNRIYINSRLAENSKIELASDHVHYVKTVLRLKVNDGLRIFNGTEGEFLAHITAIGKHNISVRLKEQLKKPYTESALT
LAVAIIKQDKLMLAINMATQLGITKIIPLITSRCQFRTVNIERLTKCVIEATEQSERLIPPIIEKAITIQDCLKKHNNFM
LYANEHEKEENSILRISSLLDNNIAIIIGPEGGFTNDELELLASYKNTKSVSLGSNILRAETAVITAIVQVKLLGRNCEE
QSDVVIQKNNKKML
>Mature_254_residues
MRFNRIYINSRLAENSKIELASDHVHYVKTVLRLKVNDGLRIFNGTEGEFLAHITAIGKHNISVRLKEQLKKPYTESALT
LAVAIIKQDKLMLAINMATQLGITKIIPLITSRCQFRTVNIERLTKCVIEATEQSERLIPPIIEKAITIQDCLKKHNNFM
LYANEHEKEENSILRISSLLDNNIAIIIGPEGGFTNDELELLASYKNTKSVSLGSNILRAETAVITAIVQVKLLGRNCEE
QSDVVIQKNNKKML

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=242, Percent_Identity=30.5785123966942, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 28649; Mature: 28649

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFNRIYINSRLAENSKIELASDHVHYVKTVLRLKVNDGLRIFNGTEGEFLAHITAIGKH
CCCEEEEEECCCCCCCCEEEECCHHHHHHHHHHEEECCCEEEEECCCCCEEEEEEECCCC
NISVRLKEQLKKPYTESALTLAVAIIKQDKLMLAINMATQLGITKIIPLITSRCQFRTVN
CEEEEEHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEEEC
IERLTKCVIEATEQSERLIPPIIEKAITIQDCLKKHNNFMLYANEHEKEENSILRISSLL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHEEEEHHH
DNNIAIIIGPEGGFTNDELELLASYKNTKSVSLGSNILRAETAVITAIVQVKLLGRNCEE
CCCEEEEECCCCCCCCHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCC
QSDVVIQKNNKKML
CCCEEEEECCCCCC
>Mature Secondary Structure
MRFNRIYINSRLAENSKIELASDHVHYVKTVLRLKVNDGLRIFNGTEGEFLAHITAIGKH
CCCEEEEEECCCCCCCCEEEECCHHHHHHHHHHEEECCCEEEEECCCCCEEEEEEECCCC
NISVRLKEQLKKPYTESALTLAVAIIKQDKLMLAINMATQLGITKIIPLITSRCQFRTVN
CEEEEEHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEEEC
IERLTKCVIEATEQSERLIPPIIEKAITIQDCLKKHNNFMLYANEHEKEENSILRISSLL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHEEEEHHH
DNNIAIIIGPEGGFTNDELELLASYKNTKSVSLGSNILRAETAVITAIVQVKLLGRNCEE
CCCEEEEECCCCCCCCHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCC
QSDVVIQKNNKKML
CCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]