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The map label for this gene is rlmE [H]

Identifier: 157825346

GI number: 157825346

Start: 220678

End: 221361

Strand: Direct

Name: rlmE [H]

Synonym: A1C_01140

Alternate gene names: 157825346

Gene position: 220678-221361 (Clockwise)

Preceding gene: 157825345

Following gene: 157825348

Centisome position: 17.93

GC content: 33.19

Gene sequence:

>684_bases
ATGACAAATAATTTAAGCGGCTATAGAAATAAATTCGTTAGAGTTAAGACTTCTAAAAAACGTACTGTTTCTTCTGGTAA
TTGGCTTAGACGCCAATTAAACGATCCATATGTTGCAAAAGCACGTATAGAAGGCTTTAGGTCGAGAGCGGCATATAAAC
TACTTGAAATTCATGAAAAATTTAAACTTTTTACTCCTAATATGAAAATTGTTGATCTAGGAGCAGCCCCTGGGGGATGG
AGCCAAGTAGCTTCCAAGCTCATTAAAGCTTCGGATAATAGCCTCAATAATAAAATAATCTCTATCGATTTGTTAGAAAT
CGAACCTATTATCGGAGTTGAGTTTCTTCAAAAAGACTTTTTTGAAGAAAATACGGAAGAATTAATTATTCAGGTTTTAG
ACGGTAAAGCTGATATAGTAATGAGTGATATGGCATCAAATACCACAGGTCATAAAGCTACAGACCACATACGAACCTTA
CTGTTATGTGAGCAAGCTTTTGAATTTGCTTTAAAAGTATTAAAGCCATCTGGTCATTTTATTGCTAAAATTTTTCGTGG
CGGTGCAGAAAATGAATTATTAAATAAAGTGAAGTGTGAATTTAAAACGGTAAAACATTTTAAACCTTCGTCCAGCCGCA
GCGAATCTACAGAAATATATTTAGTTGCTATAAATAAAAAATAA

Upstream 100 bases:

>100_bases
ACGAATATACGGATATAGCAAATGATATGTTAAATGGATACGAGATCGGTTTTGTTAATTCGGTATTAGACACAATAGCC
AAAGAGAAGAATAAAATTTC

Downstream 100 bases:

>100_bases
CATATATAAATTTACTATATATATGTTATTTTTTTATTTAACATACTAGATGATAATTACTTATATCCCCCGTTGTTTTG
ACTTCCTGAGCATATTCTTG

Product: ribosomal RNA large subunit methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MTNNLSGYRNKFVRVKTSKKRTVSSGNWLRRQLNDPYVAKARIEGFRSRAAYKLLEIHEKFKLFTPNMKIVDLGAAPGGW
SQVASKLIKASDNSLNNKIISIDLLEIEPIIGVEFLQKDFFEENTEELIIQVLDGKADIVMSDMASNTTGHKATDHIRTL
LLCEQAFEFALKVLKPSGHFIAKIFRGGAENELLNKVKCEFKTVKHFKPSSSRSESTEIYLVAINKK

Sequences:

>Translated_227_residues
MTNNLSGYRNKFVRVKTSKKRTVSSGNWLRRQLNDPYVAKARIEGFRSRAAYKLLEIHEKFKLFTPNMKIVDLGAAPGGW
SQVASKLIKASDNSLNNKIISIDLLEIEPIIGVEFLQKDFFEENTEELIIQVLDGKADIVMSDMASNTTGHKATDHIRTL
LLCEQAFEFALKVLKPSGHFIAKIFRGGAENELLNKVKCEFKTVKHFKPSSSRSESTEIYLVAINKK
>Mature_226_residues
TNNLSGYRNKFVRVKTSKKRTVSSGNWLRRQLNDPYVAKARIEGFRSRAAYKLLEIHEKFKLFTPNMKIVDLGAAPGGWS
QVASKLIKASDNSLNNKIISIDLLEIEPIIGVEFLQKDFFEENTEELIIQVLDGKADIVMSDMASNTTGHKATDHIRTLL
LCEQAFEFALKVLKPSGHFIAKIFRGGAENELLNKVKCEFKTVKHFKPSSSRSESTEIYLVAINKK

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=211, Percent_Identity=37.914691943128, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI29029591, Length=191, Percent_Identity=38.2198952879581, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI29029589, Length=191, Percent_Identity=38.2198952879581, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI7110661, Length=191, Percent_Identity=38.2198952879581, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI194097365, Length=191, Percent_Identity=34.5549738219895, Blast_Score=104, Evalue=6e-23,
Organism=Escherichia coli, GI1789569, Length=212, Percent_Identity=45.7547169811321, Blast_Score=187, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17553474, Length=214, Percent_Identity=35.5140186915888, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17554650, Length=196, Percent_Identity=37.7551020408163, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17553860, Length=193, Percent_Identity=36.2694300518135, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71987550, Length=194, Percent_Identity=36.0824742268041, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71987561, Length=193, Percent_Identity=36.2694300518135, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321302, Length=282, Percent_Identity=29.4326241134752, Blast_Score=120, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319535, Length=196, Percent_Identity=37.2448979591837, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319796, Length=191, Percent_Identity=34.5549738219895, Blast_Score=99, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21356387, Length=214, Percent_Identity=37.3831775700935, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24647580, Length=196, Percent_Identity=37.7551020408163, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24648639, Length=188, Percent_Identity=39.3617021276596, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI18859957, Length=190, Percent_Identity=35.7894736842105, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 25664; Mature: 25533

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNLSGYRNKFVRVKTSKKRTVSSGNWLRRQLNDPYVAKARIEGFRSRAAYKLLEIHEK
CCCCCCCHHCCEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FKLFTPNMKIVDLGAAPGGWSQVASKLIKASDNSLNNKIISIDLLEIEPIIGVEFLQKDF
HHEECCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEEECCHHHHHHHHHHH
FEENTEELIIQVLDGKADIVMSDMASNTTGHKATDHIRTLLLCEQAFEFALKVLKPSGHF
HCCCHHHHHHHHHCCCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
IAKIFRGGAENELLNKVKCEFKTVKHFKPSSSRSESTEIYLVAINKK
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure 
TNNLSGYRNKFVRVKTSKKRTVSSGNWLRRQLNDPYVAKARIEGFRSRAAYKLLEIHEK
CCCCCCHHCCEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FKLFTPNMKIVDLGAAPGGWSQVASKLIKASDNSLNNKIISIDLLEIEPIIGVEFLQKDF
HHEECCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEEECCHHHHHHHHHHH
FEENTEELIIQVLDGKADIVMSDMASNTTGHKATDHIRTLLLCEQAFEFALKVLKPSGHF
HCCCHHHHHHHHHCCCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
IAKIFRGGAENELLNKVKCEFKTVKHFKPSSSRSESTEIYLVAINKK
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA