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The map label for this gene is queF [H]

Identifier: 157825232

GI number: 157825232

Start: 92929

End: 93750

Strand: Direct

Name: queF [H]

Synonym: A1C_00555

Alternate gene names: 157825232

Gene position: 92929-93750 (Clockwise)

Preceding gene: 157825231

Following gene: 157825236

Centisome position: 7.55

GC content: 30.66

Gene sequence:

>822_bases
ATGCCTTTATCTACTTCTTTGCTTGGTAAAACAAGTACCTATAAAGATAGTTATGATGCGACTTTATTATTTAAAATTCC
ACGTATAAATAATCGAAACGCACTTGGAATAAATAGTAATAATCTTCCGTTTTACGGTGTAGATATTTGGAATACATATG
AACTATCTTGTATTAATAAGAAAGGTAAGCCATGGGTTGGAGTAGGCACTTTTTATATACCGACGGATTCTGAAAACATA
GTAGAATCAAAATCATTTAAACTATATCTAAATTCTTTTAATAATTTTGTTGTTGAATCAGTAGAAGAACTAGAAAGAAT
TATCTTGCAGGATCTAAGTAATGTTACACACGCTAAAGTAACAGGACGCATATTTCCTATAAATACAAAAATAGCATTTG
GTGTTCCAAGCGGTAAAAATATCGATGCTTTAGATATAGTATGCAATAATTACGGTCCGCCCGATAATAGTTTAGTTGAG
TATGAAGAGGTTTTAGTAGAAGAAGAGATTAATTCTAATTTACTAAAATCAAATTGCTTAGTCACTGGTCAACCTGATTG
GGGTTCAATCGTTATAAAATATAAAGGAAAAAAGTTAAAACATGATTCTTTTCTGAAATATTTAATATCTTTTAGAAATT
ATCATGAGTTTGCAGAGCAGTGTGCTGAGCGTATTTTTACAGATATTAAAAATGCTATAAAGCCTGACTTTCTTTCTCTT
TATATAGTATATACTAGGCGCGGAGGAATTGATATTTGTCCCTATCGTTCTACCGACAAAAGTTATAGTTTACCTAGCGA
TAAACGACTTATTAGGCAATAA

Upstream 100 bases:

>100_bases
CGGTAGAGATTATATCGACACAGTATGGGGACGCGGTTATATGCTAAAAGAATATGATGAATTACAACAAAAAGAAATTT
TAGCACAAGGAGCATAATTA

Downstream 100 bases:

>100_bases
TCACTTTTCTAATCCATAAAAAATCCACTCGATGTCATATAGTGGTCAAAGCCACGGTGTTCAAAAACAACTTAAAATAC
TAATAGCTTTAGTATTTTAG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MPLSTSLLGKTSTYKDSYDATLLFKIPRINNRNALGINSNNLPFYGVDIWNTYELSCINKKGKPWVGVGTFYIPTDSENI
VESKSFKLYLNSFNNFVVESVEELERIILQDLSNVTHAKVTGRIFPINTKIAFGVPSGKNIDALDIVCNNYGPPDNSLVE
YEEVLVEEEINSNLLKSNCLVTGQPDWGSIVIKYKGKKLKHDSFLKYLISFRNYHEFAEQCAERIFTDIKNAIKPDFLSL
YIVYTRRGGIDICPYRSTDKSYSLPSDKRLIRQ

Sequences:

>Translated_273_residues
MPLSTSLLGKTSTYKDSYDATLLFKIPRINNRNALGINSNNLPFYGVDIWNTYELSCINKKGKPWVGVGTFYIPTDSENI
VESKSFKLYLNSFNNFVVESVEELERIILQDLSNVTHAKVTGRIFPINTKIAFGVPSGKNIDALDIVCNNYGPPDNSLVE
YEEVLVEEEINSNLLKSNCLVTGQPDWGSIVIKYKGKKLKHDSFLKYLISFRNYHEFAEQCAERIFTDIKNAIKPDFLSL
YIVYTRRGGIDICPYRSTDKSYSLPSDKRLIRQ
>Mature_272_residues
PLSTSLLGKTSTYKDSYDATLLFKIPRINNRNALGINSNNLPFYGVDIWNTYELSCINKKGKPWVGVGTFYIPTDSENIV
ESKSFKLYLNSFNNFVVESVEELERIILQDLSNVTHAKVTGRIFPINTKIAFGVPSGKNIDALDIVCNNYGPPDNSLVEY
EEVLVEEEINSNLLKSNCLVTGQPDWGSIVIKYKGKKLKHDSFLKYLISFRNYHEFAEQCAERIFTDIKNAIKPDFLSLY
IVYTRRGGIDICPYRSTDKSYSLPSDKRLIRQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=277, Percent_Identity=45.1263537906137, Blast_Score=239, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 31112; Mature: 30981

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSTSLLGKTSTYKDSYDATLLFKIPRINNRNALGINSNNLPFYGVDIWNTYELSCINK
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCCEEEEEECCEEEEEEECC
KGKPWVGVGTFYIPTDSENIVESKSFKLYLNSFNNFVVESVEELERIILQDLSNVTHAKV
CCCCEEEEEEEEEECCCCHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
TGRIFPINTKIAFGVPSGKNIDALDIVCNNYGPPDNSLVEYEEVLVEEEINSNLLKSNCL
EEEEEEECCEEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCE
VTGQPDWGSIVIKYKGKKLKHDSFLKYLISFRNYHEFAEQCAERIFTDIKNAIKPDFLSL
EECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
YIVYTRRGGIDICPYRSTDKSYSLPSDKRLIRQ
EEEEEECCCEEECCCCCCCCCCCCCCHHHHHCC
>Mature Secondary Structure 
PLSTSLLGKTSTYKDSYDATLLFKIPRINNRNALGINSNNLPFYGVDIWNTYELSCINK
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCCEEEEEECCEEEEEEECC
KGKPWVGVGTFYIPTDSENIVESKSFKLYLNSFNNFVVESVEELERIILQDLSNVTHAKV
CCCCEEEEEEEEEECCCCHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
TGRIFPINTKIAFGVPSGKNIDALDIVCNNYGPPDNSLVEYEEVLVEEEINSNLLKSNCL
EEEEEEECCEEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCE
VTGQPDWGSIVIKYKGKKLKHDSFLKYLISFRNYHEFAEQCAERIFTDIKNAIKPDFLSL
EECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
YIVYTRRGGIDICPYRSTDKSYSLPSDKRLIRQ
EEEEEECCCEEECCCCCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA