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The map label for this gene is glnG [H]

Identifier: 157373114

GI number: 157373114

Start: 5411333

End: 5412745

Strand: Direct

Name: glnG [H]

Synonym: Spro_4883

Alternate gene names: 157373114

Gene position: 5411333-5412745 (Clockwise)

Preceding gene: 157373113

Following gene: 157373117

Centisome position: 99.31

GC content: 59.02

Gene sequence:

>1413_bases
ATGCAACGAGGGATAGTCTGGATCGTCGATGACGATAGCTCCATCCGCTGGGTGCTTGAACGCGCGCTCACTGGAGCGGG
TGTGAGTTGCGTGACCTTTGAAAATGGCAATGCGGTACTGGAAGCCCTGGCCACGCAAACTCCTGACGTGTTGCTGTCTG
ACATCCGCATGCCGGGTATGGATGGTCTGGCGTTGCTCAAGCAGATCAAGCAGCGTCACCCCATGCTTCCGGTCATCATA
ATGACGGCACATTCGGATTTGGACGCCGCGGTCAGCGCCTACCAGCAAGGAGCGTTCGATTACCTGCCAAAACCCTTTGA
TATCGATGAAGCGGTCGCGCTGGTAGAGCGCGCCATCAGCCATTATCAGGAACAACAACAACCGGCACGCAGCCAGCCGG
CCAACGACCCGGTGGCGGACATTATCGGCGAAGCCCCGGCGATGCAGGACGTGTTCCGCATCATTGGCCGTCTGTCGCGT
TCATCGATCAGCGTGCTGATCAACGGCGAATCCGGCACCGGTAAAGAGCTGGTGGCCCATGCCCTGCATCGCCACAGCCC
GCGAGCCAAATCTCCGTTTATCGCGTTGAATATGGCGGCGATCCCTAAAGACCTGATCGAATCCGAGCTGTTCGGCCACG
AGAAAGGGGCTTTTACCGGTGCCAATCAGATCCGTCAGGGCCGTTTTGAGCAGGCCGACGGCGGAACGCTGTTCCTGGAT
GAAATCGGCGATATGCCGCTGGATGTTCAAACCCGTTTACTGCGGGTGCTGGCCGATGGTCAATTCTACCGGGTCGGTGG
ATACGCGCCGGTGAAGGTGGACGTGCGCATTATCGCCGCCACCCATCAGAACCTGGAGCTGCGGGTACAGGAAGGTAAAT
TCCGTGAAGATCTGTTCCATCGCCTGAACGTCATTCGCGTACATCTGCCACCGTTGCGTGAACGTCGTGAGGACATACCT
CGTCTGGCGCGCTACTTCCTGCAGGTGGCGGCAAAAGAGCTGGGCGTTGAGGCGAAGAATCTGCATCCGGAAACTGAAAC
CGCCCTGACCCGCCTGCCGTGGCCAGGTAACGTGCGTCAGTTGGAAAACACCTGTCGCTGGTTAACCGTGATGGCCGCCG
GGCAGGAAGTGCTGATTCAGGATCTGCCGAGTGAGCTGTTTGAAACCGCTGCGCCGGAAACGGCCGGCCACAGCTTGCCG
GACAGTTGGGCTACGCTACTGGCTCAGTGGGCCGACCGGGCACTGCGTTCCGGTCATCAAAACTTGCTGTCGGAAGCGCA
ACCGGAAATGGAGCGCACCTTGCTCACCACCGCGCTGCGCCATACCCAGGGGCATAAGCAGGAAGCCGCTCGCTTGCTGG
GTTGGGGCCGCAATACGTTAACGCGCAAGTTGAAAGAGTTGGGAATGGAATAG

Upstream 100 bases:

>100_bases
GTAATCTTATCGATCAGCATTGCGGAAAAATTGAATTTAACAGTTGGCCAGGACATACCGAATTCTCGGTTTACCTGCCT
ATTCGCCAGTGAGGTTTTCT

Downstream 100 bases:

>100_bases
TTTGCGTTGTTGATTATAAAAAACATAATCAAGCGCACAAAAAATCGACGGTTTCAGGCTTTACAGCCGCTCTGCCGGCA
GTATGATCGGGGCGCACACT

Product: nitrogen regulation protein NR(I)

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MQRGIVWIVDDDSSIRWVLERALTGAGVSCVTFENGNAVLEALATQTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPARSQPANDPVADIIGEAPAMQDVFRIIGRLSR
SSISVLINGESGTGKELVAHALHRHSPRAKSPFIALNMAAIPKDLIESELFGHEKGAFTGANQIRQGRFEQADGGTLFLD
EIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLELRVQEGKFREDLFHRLNVIRVHLPPLRERREDIP
RLARYFLQVAAKELGVEAKNLHPETETALTRLPWPGNVRQLENTCRWLTVMAAGQEVLIQDLPSELFETAAPETAGHSLP
DSWATLLAQWADRALRSGHQNLLSEAQPEMERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME

Sequences:

>Translated_470_residues
MQRGIVWIVDDDSSIRWVLERALTGAGVSCVTFENGNAVLEALATQTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPARSQPANDPVADIIGEAPAMQDVFRIIGRLSR
SSISVLINGESGTGKELVAHALHRHSPRAKSPFIALNMAAIPKDLIESELFGHEKGAFTGANQIRQGRFEQADGGTLFLD
EIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLELRVQEGKFREDLFHRLNVIRVHLPPLRERREDIP
RLARYFLQVAAKELGVEAKNLHPETETALTRLPWPGNVRQLENTCRWLTVMAAGQEVLIQDLPSELFETAAPETAGHSLP
DSWATLLAQWADRALRSGHQNLLSEAQPEMERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME
>Mature_470_residues
MQRGIVWIVDDDSSIRWVLERALTGAGVSCVTFENGNAVLEALATQTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPARSQPANDPVADIIGEAPAMQDVFRIIGRLSR
SSISVLINGESGTGKELVAHALHRHSPRAKSPFIALNMAAIPKDLIESELFGHEKGAFTGANQIRQGRFEQADGGTLFLD
EIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLELRVQEGKFREDLFHRLNVIRVHLPPLRERREDIP
RLARYFLQVAAKELGVEAKNLHPETETALTRLPWPGNVRQLENTCRWLTVMAAGQEVLIQDLPSELFETAAPETAGHSLP
DSWATLLAQWADRALRSGHQNLLSEAQPEMERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=470, Percent_Identity=92.1276595744681, Blast_Score=883, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=468, Percent_Identity=42.5213675213675, Blast_Score=370, Evalue=1e-103,
Organism=Escherichia coli, GI1790437, Length=463, Percent_Identity=42.548596112311, Blast_Score=347, Evalue=7e-97,
Organism=Escherichia coli, GI1788905, Length=462, Percent_Identity=38.961038961039, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI87082117, Length=353, Percent_Identity=41.643059490085, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI1789233, Length=348, Percent_Identity=42.816091954023, Blast_Score=248, Evalue=9e-67,
Organism=Escherichia coli, GI1789087, Length=335, Percent_Identity=42.3880597014925, Blast_Score=238, Evalue=8e-64,
Organism=Escherichia coli, GI1786524, Length=347, Percent_Identity=42.9394812680115, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1787583, Length=331, Percent_Identity=40.785498489426, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI87082152, Length=334, Percent_Identity=41.6167664670659, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI87081872, Length=332, Percent_Identity=40.3614457831325, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI87081858, Length=312, Percent_Identity=31.4102564102564, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1789828, Length=236, Percent_Identity=34.3220338983051, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1790860, Length=129, Percent_Identity=34.1085271317829, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789809, Length=124, Percent_Identity=29.8387096774194, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1786911, Length=136, Percent_Identity=31.6176470588235, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789402, Length=111, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787487, Length=100, Percent_Identity=34, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52312; Mature: 52312

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRGIVWIVDDDSSIRWVLERALTGAGVSCVTFENGNAVLEALATQTPDVLLSDIRMPGM
CCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHCCCCCC
DGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAIS
CHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHH
HYQEQQQPARSQPANDPVADIIGEAPAMQDVFRIIGRLSRSSISVLINGESGTGKELVAH
HHHHHHCCHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
ALHRHSPRAKSPFIALNMAAIPKDLIESELFGHEKGAFTGANQIRQGRFEQADGGTLFLD
HHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEEHH
EIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLELRVQEGKFREDLFH
HHCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEEECCCCEEEEECCCHHHHHHH
RLNVIRVHLPPLRERREDIPRLARYFLQVAAKELGVEAKNLHPETETALTRLPWPGNVRQ
HHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHCCCCCCCHHH
LENTCRWLTVMAAGQEVLIQDLPSELFETAAPETAGHSLPDSWATLLAQWADRALRSGHQ
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
NLLSEAQPEMERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME
HHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MQRGIVWIVDDDSSIRWVLERALTGAGVSCVTFENGNAVLEALATQTPDVLLSDIRMPGM
CCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHCCCCCC
DGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAIS
CHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHH
HYQEQQQPARSQPANDPVADIIGEAPAMQDVFRIIGRLSRSSISVLINGESGTGKELVAH
HHHHHHCCHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
ALHRHSPRAKSPFIALNMAAIPKDLIESELFGHEKGAFTGANQIRQGRFEQADGGTLFLD
HHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEEHH
EIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLELRVQEGKFREDLFH
HHCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEEECCCCEEEEECCCHHHHHHH
RLNVIRVHLPPLRERREDIPRLARYFLQVAAKELGVEAKNLHPETETALTRLPWPGNVRQ
HHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHCCCCCCCHHH
LENTCRWLTVMAAGQEVLIQDLPSELFETAAPETAGHSLPDSWATLLAQWADRALRSGHQ
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
NLLSEAQPEMERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME
HHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]