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The map label for this gene is aroE

Identifier: 157372739

GI number: 157372739

Start: 5000107

End: 5000925

Strand: Reverse

Name: aroE

Synonym: Spro_4506

Alternate gene names: 157372739

Gene position: 5000925-5000107 (Counterclockwise)

Preceding gene: 157372740

Following gene: 157372737

Centisome position: 91.78

GC content: 54.58

Gene sequence:

>819_bases
ATGGAGAAGTTTGCAGTATTCGGTAATCCTATCGGCCACAGCAAGTCACCGCGTATTCATGCGCTATTTGCCGCGCAAAC
AGGGATAGAACATCCTTATGGCACTGTGCTGGCGCCACTGGATGGTTTCGAAACCACTTTGCAGACGTTTATTCAGGCGG
GTGGCAAGGGAGCGAACGTCACGGTGCCTTTTAAAGAAAATGCTTATGAAGCGGCGACCGAACTGAGTCAACGAGCCTCG
CTGGCCGGTGCGGTAAATACCTTGAAGATACTGCCAGACGGTGGGTTACTGGGTGACAACACTGATGGGATCGGCCTGCT
GACCGATCTTGAACGTCAGGGGTTGATCCAACCCAAGGATCGTATCTTGTTGGTGGGGGCTGGTGGCGCGGCACGTGGCG
CGATCTTGCCGCTACTGTCTTTTGGTTGTGAAGTGGTGATCACCAACCGTACTTTTAGCCGTGCGCAGACATTGGCGCAG
GTATTCCAGCATCTGGGCGAAATATCCGCGTTACCCCTGGATCAACTTGATCAACAGCGGTTTGATTTAGTGATTAACGC
TACGGCATCCGGTATCAGCGGTGACATCCCGGCGCTACCGGTTGGTACGGTCAATGGCCATACGCGTTGTTATGACATGT
TTTATCAGCAGGGATTAACTCCCTTTCTGGCCTGGGCGCAGCAACAGGGTGCCACAGAATATGCCGACGGTTTAGGGATG
CTGGTGGGGCAGGCCGCGCATGCGTTCCTGCTGTGGCATGGTGTGATGCCGGAGATCGAACCGGTATTGCGCCAGCTGCG
TCACGAACTGGCAGTGTAA

Upstream 100 bases:

>100_bases
TCCCGCTGCTGGCCGGTAGCGTCGGTGGCCGTCTTAACCCTTCCGAAATCAGAGATGTCCTGACCGGTGAGCAGATCCGT
CAAGGCTAGAGGCAACGTGC

Downstream 100 bases:

>100_bases
CTCTGAGCCCGTTCCCCTGAGCGGGAACGGGCGCCTGTCAGATATCCTCTGACAAATATTCGTCTTTCCAACGTACATAG
TTAGTAGAAGAGTAAAGCAA

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MEKFAVFGNPIGHSKSPRIHALFAAQTGIEHPYGTVLAPLDGFETTLQTFIQAGGKGANVTVPFKENAYEAATELSQRAS
LAGAVNTLKILPDGGLLGDNTDGIGLLTDLERQGLIQPKDRILLVGAGGAARGAILPLLSFGCEVVITNRTFSRAQTLAQ
VFQHLGEISALPLDQLDQQRFDLVINATASGISGDIPALPVGTVNGHTRCYDMFYQQGLTPFLAWAQQQGATEYADGLGM
LVGQAAHAFLLWHGVMPEIEPVLRQLRHELAV

Sequences:

>Translated_272_residues
MEKFAVFGNPIGHSKSPRIHALFAAQTGIEHPYGTVLAPLDGFETTLQTFIQAGGKGANVTVPFKENAYEAATELSQRAS
LAGAVNTLKILPDGGLLGDNTDGIGLLTDLERQGLIQPKDRILLVGAGGAARGAILPLLSFGCEVVITNRTFSRAQTLAQ
VFQHLGEISALPLDQLDQQRFDLVINATASGISGDIPALPVGTVNGHTRCYDMFYQQGLTPFLAWAQQQGATEYADGLGM
LVGQAAHAFLLWHGVMPEIEPVLRQLRHELAV
>Mature_272_residues
MEKFAVFGNPIGHSKSPRIHALFAAQTGIEHPYGTVLAPLDGFETTLQTFIQAGGKGANVTVPFKENAYEAATELSQRAS
LAGAVNTLKILPDGGLLGDNTDGIGLLTDLERQGLIQPKDRILLVGAGGAARGAILPLLSFGCEVVITNRTFSRAQTLAQ
VFQHLGEISALPLDQLDQQRFDLVINATASGISGDIPALPVGTVNGHTRCYDMFYQQGLTPFLAWAQQQGATEYADGLGM
LVGQAAHAFLLWHGVMPEIEPVLRQLRHELAV

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1789675, Length=271, Percent_Identity=65.6826568265683, Blast_Score=373, Evalue=1e-105,
Organism=Escherichia coli, GI1787983, Length=276, Percent_Identity=31.1594202898551, Blast_Score=89, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320332, Length=281, Percent_Identity=28.1138790035587, Blast_Score=89, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_SERP5 (A8GKG0)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480728.1
- ProteinModelPortal:   A8GKG0
- SMR:   A8GKG0
- STRING:   A8GKG0
- GeneID:   5605842
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4506
- eggNOG:   COG0169
- HOGENOM:   HBG553408
- OMA:   FAAQTGI
- ProtClustDB:   PRK00258
- BioCyc:   SPRO399741:SPRO_4506-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 29022; Mature: 29022

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: ACT_SITE 65-65

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFAVFGNPIGHSKSPRIHALFAAQTGIEHPYGTVLAPLDGFETTLQTFIQAGGKGANV
CCCEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCEE
TVPFKENAYEAATELSQRASLAGAVNTLKILPDGGLLGDNTDGIGLLTDLERQGLIQPKD
EEECCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCEEEECHHHCCCCCCCC
RILLVGAGGAARGAILPLLSFGCEVVITNRTFSRAQTLAQVFQHLGEISALPLDQLDQQR
CEEEEECCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
FDLVINATASGISGDIPALPVGTVNGHTRCYDMFYQQGLTPFLAWAQQQGATEYADGLGM
EEEEEECCCCCCCCCCCCCEECCCCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHH
LVGQAAHAFLLWHGVMPEIEPVLRQLRHELAV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKFAVFGNPIGHSKSPRIHALFAAQTGIEHPYGTVLAPLDGFETTLQTFIQAGGKGANV
CCCEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCEE
TVPFKENAYEAATELSQRASLAGAVNTLKILPDGGLLGDNTDGIGLLTDLERQGLIQPKD
EEECCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCEEEECHHHCCCCCCCC
RILLVGAGGAARGAILPLLSFGCEVVITNRTFSRAQTLAQVFQHLGEISALPLDQLDQQR
CEEEEECCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
FDLVINATASGISGDIPALPVGTVNGHTRCYDMFYQQGLTPFLAWAQQQGATEYADGLGM
EEEEEECCCCCCCCCCCCCEECCCCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHH
LVGQAAHAFLLWHGVMPEIEPVLRQLRHELAV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA