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The map label for this gene is aceA [H]

Identifier: 157372735

GI number: 157372735

Start: 4989151

End: 4990458

Strand: Reverse

Name: aceA [H]

Synonym: Spro_4502

Alternate gene names: 157372735

Gene position: 4990458-4989151 (Counterclockwise)

Preceding gene: 157372736

Following gene: 157372734

Centisome position: 91.59

GC content: 58.79

Gene sequence:

>1308_bases
ATGTCTACCTCTCGTGCTCAACAGATTCAACAACTGGAACAGGAATGGAAATCGGCTCGCTGGGAAGGCATCACCCGCCC
GTACAGCGCGGAAGACGTCATTAACCTGCGCGGTTCGGTTAACCCGGTTTGCACCCTGGCACAGAACGGTGCCGCCAAAC
TGTGGGAACTGCTGCACGGTAAATCACGCAAAGGCTACGTCAACAGTCTGGGGGCGCTCACTGGCGGCCAGGCACTGCAG
CAGGCCAAGGCCGGCATCGAGGCGATTTACCTGTCCGGTTGGCAGGTGGCGGCGGATGCCAACACGGCGTCTGCGATGTA
CCCGGACCAGTCGCTGTACCCGGTTGACTCTGTGCCGGGTGTGGTTGAGCGCATCAACAACACCTTCCGCCGCGCCGACC
AGATCCAGTGGGCCAACAAAATTGAGCCGGGCAGCAAAGGTTACACCGACTACTTCCTGCCGATCGTCGCCGATGCCGAA
GCCGGCTTTGGTGGCGTGCTGAACGCTTTCGAACTGATGAAGGCGATGATTGAGGCCGGTGCTGCCGGGGTGCACTTCGA
AGACCAACTGGCGGCGGTGAAAAAGTGCGGTCATATGGGCGGCAAAGTGCTGGTGCCAACCCAGGAAGCGATCCAGAAAT
TGGTTGCTGCGCGCCTGGCGGCCGATGTGCTCGGCGTGCCGACGTTAGTCATCGCACGTACCGATGCCGATGCCGCCGAT
TTACTGACTTCTGACTGCGACCCGTACGACCGCGAGTTTGTGTCCGGTGAGCGCACTGCCGAAGGTTTCTTCCGCACCCA
TGCCGGTGTCGAGCAAGCGATCAGCCGTGGCCTGGCATATTGCCCTTACGCTGACCTGGTGTGGTGCGAAACCTCCACGC
CGGATCTGCAGGCGGCCAAGCGCTTTGCCGATGCGATCCATGCCAAATTCCCGGGCAAACTGCTGGCGTACAACTGCTCG
CCGTCATTCAACTGGAAAAAGAACCTGGACGACCAGACTATTGCTCGCTTCCAGGAAGAGCTGTCGGCGATGGGCTACAA
GTACCAGTTCATTACGCTAGCGGGCATCCACAGCATGTGGTTCAACATGTTTGACCTGGCGCATGCCTATGCACAGGGCG
AAGGCATGAAACACTACGTCGAAAAAGTGCAGCAGCCAGAGTTCGACGCGGTGTCTCGCGGTTATACCTTTGCCTCACAC
CAACAGGAAGTGGGTACCGGTTACTTCGACAAGGTGACCACCATTATTCAGGGCGGTGCTTCCTCAGTGACGGCATTGAC
CGGTTCTACGGAAGAACAGCAGTTCTGA

Upstream 100 bases:

>100_bases
AAGACGAATTAATCGATTTCCTGACTTTACCTGGCTATGAGCTACTGGCCTGAATCCGTTCCTGACCATTAACCCTACAT
TATTGAAAAGGATAAAAATT

Downstream 100 bases:

>100_bases
TGCAGTCACGCTGACGATAGTTAACTCTGAGCCTGCTTATGCAGGCTCTCTTTTCTGGCGGGAGGTAAGGATGGCGGCAA
AACTGGAGCTGTTGATCGCG

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSTSRAQQIQQLEQEWKSARWEGITRPYSAEDVINLRGSVNPVCTLAQNGAAKLWELLHGKSRKGYVNSLGALTGGQALQ
QAKAGIEAIYLSGWQVAADANTASAMYPDQSLYPVDSVPGVVERINNTFRRADQIQWANKIEPGSKGYTDYFLPIVADAE
AGFGGVLNAFELMKAMIEAGAAGVHFEDQLAAVKKCGHMGGKVLVPTQEAIQKLVAARLAADVLGVPTLVIARTDADAAD
LLTSDCDPYDREFVSGERTAEGFFRTHAGVEQAISRGLAYCPYADLVWCETSTPDLQAAKRFADAIHAKFPGKLLAYNCS
PSFNWKKNLDDQTIARFQEELSAMGYKYQFITLAGIHSMWFNMFDLAHAYAQGEGMKHYVEKVQQPEFDAVSRGYTFASH
QQEVGTGYFDKVTTIIQGGASSVTALTGSTEEQQF

Sequences:

>Translated_435_residues
MSTSRAQQIQQLEQEWKSARWEGITRPYSAEDVINLRGSVNPVCTLAQNGAAKLWELLHGKSRKGYVNSLGALTGGQALQ
QAKAGIEAIYLSGWQVAADANTASAMYPDQSLYPVDSVPGVVERINNTFRRADQIQWANKIEPGSKGYTDYFLPIVADAE
AGFGGVLNAFELMKAMIEAGAAGVHFEDQLAAVKKCGHMGGKVLVPTQEAIQKLVAARLAADVLGVPTLVIARTDADAAD
LLTSDCDPYDREFVSGERTAEGFFRTHAGVEQAISRGLAYCPYADLVWCETSTPDLQAAKRFADAIHAKFPGKLLAYNCS
PSFNWKKNLDDQTIARFQEELSAMGYKYQFITLAGIHSMWFNMFDLAHAYAQGEGMKHYVEKVQQPEFDAVSRGYTFASH
QQEVGTGYFDKVTTIIQGGASSVTALTGSTEEQQF
>Mature_434_residues
STSRAQQIQQLEQEWKSARWEGITRPYSAEDVINLRGSVNPVCTLAQNGAAKLWELLHGKSRKGYVNSLGALTGGQALQQ
AKAGIEAIYLSGWQVAADANTASAMYPDQSLYPVDSVPGVVERINNTFRRADQIQWANKIEPGSKGYTDYFLPIVADAEA
GFGGVLNAFELMKAMIEAGAAGVHFEDQLAAVKKCGHMGGKVLVPTQEAIQKLVAARLAADVLGVPTLVIARTDADAADL
LTSDCDPYDREFVSGERTAEGFFRTHAGVEQAISRGLAYCPYADLVWCETSTPDLQAAKRFADAIHAKFPGKLLAYNCSP
SFNWKKNLDDQTIARFQEELSAMGYKYQFITLAGIHSMWFNMFDLAHAYAQGEGMKHYVEKVQQPEFDAVSRGYTFASHQ
QEVGTGYFDKVTTIIQGGASSVTALTGSTEEQQF

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=432, Percent_Identity=85.1851851851852, Blast_Score=788, Evalue=0.0,
Organism=Escherichia coli, GI1786525, Length=257, Percent_Identity=29.9610894941634, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17561814, Length=437, Percent_Identity=55.1487414187643, Blast_Score=478, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI71982926, Length=437, Percent_Identity=55.1487414187643, Blast_Score=478, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6320908, Length=254, Percent_Identity=38.5826771653543, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6325263, Length=253, Percent_Identity=39.1304347826087, Blast_Score=173, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 47503; Mature: 47372

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00161 ISOCITRATE_LYASE ; PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSRAQQIQQLEQEWKSARWEGITRPYSAEDVINLRGSVNPVCTLAQNGAAKLWELLHG
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEECCCCHHHHHHHHCC
KSRKGYVNSLGALTGGQALQQAKAGIEAIYLSGWQVAADANTASAMYPDQSLYPVDSVPG
CCCCCHHHHHHHHCCHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCH
VVERINNTFRRADQIQWANKIEPGSKGYTDYFLPIVADAEAGFGGVLNAFELMKAMIEAG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCC
AAGVHFEDQLAAVKKCGHMGGKVLVPTQEAIQKLVAARLAADVLGVPTLVIARTDADAAD
CCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHH
LLTSDCDPYDREFVSGERTAEGFFRTHAGVEQAISRGLAYCPYADLVWCETSTPDLQAAK
HHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHH
RFADAIHAKFPGKLLAYNCSPSFNWKKNLDDQTIARFQEELSAMGYKYQFITLAGIHSMW
HHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHH
FNMFDLAHAYAQGEGMKHYVEKVQQPEFDAVSRGYTFASHQQEVGTGYFDKVTTIIQGGA
HHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHCCC
SSVTALTGSTEEQQF
CCEEEECCCCCCCCC
>Mature Secondary Structure 
STSRAQQIQQLEQEWKSARWEGITRPYSAEDVINLRGSVNPVCTLAQNGAAKLWELLHG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEECCCCHHHHHHHHCC
KSRKGYVNSLGALTGGQALQQAKAGIEAIYLSGWQVAADANTASAMYPDQSLYPVDSVPG
CCCCCHHHHHHHHCCHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCH
VVERINNTFRRADQIQWANKIEPGSKGYTDYFLPIVADAEAGFGGVLNAFELMKAMIEAG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCC
AAGVHFEDQLAAVKKCGHMGGKVLVPTQEAIQKLVAARLAADVLGVPTLVIARTDADAAD
CCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHH
LLTSDCDPYDREFVSGERTAEGFFRTHAGVEQAISRGLAYCPYADLVWCETSTPDLQAAK
HHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHH
RFADAIHAKFPGKLLAYNCSPSFNWKKNLDDQTIARFQEELSAMGYKYQFITLAGIHSMW
HHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHH
FNMFDLAHAYAQGEGMKHYVEKVQQPEFDAVSRGYTFASHQQEVGTGYFDKVTTIIQGGA
HHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHCCC
SSVTALTGSTEEQQF
CCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]