Click here to switch to the map view.

The map label for this gene is aceK

Identifier: 157372734

GI number: 157372734

Start: 4987326

End: 4989080

Strand: Reverse

Name: aceK

Synonym: Spro_4501

Alternate gene names: 157372734

Gene position: 4989080-4987326 (Counterclockwise)

Preceding gene: 157372735

Following gene: 157372723

Centisome position: 91.56

GC content: 56.81

Gene sequence:

>1755_bases
ATGGCGGCAAAACTGGAGCTGTTGATCGCGCAGACGATCCTGCAGGGCTTTGATGCACAGTACGGCCGCTTCCTGGAGGT
GACCGCCGGTGCGCAGCAGCGTTTTGAGCGGGCGGACTGGCCGGCAGTACAGCAGGCGATGAAAAAACGAATCCACCTGT
ACGATCATCACGTCGGCCTGGTGGTAGAGCAGCTGAAGTGCATCACCGGGCAACAGTATTTTGATGCAGACTTCCCCAGT
CGGGTGAAGGCGGTGTATACCGATCTGCTGCCGGAGTACCCACGTTTTGAGATTGCCGAAAGCTTCTTCAATTCGGTCTA
CTGTCGGCTGTTCAGACATCGCGACCTGACGCCGGAAAAACTGTTTGTTTTCAGCTCTCAGCCGGAAGGGCGCTTTCGCG
ACATCCCGCGTCCATTATCACGCGATTTCACCGCCAACGGTGACGTTTCGGCAATGTTGTACAACCTGCTGACCGATTTG
CCTCTGCGGCTGCCGTGGGAAAACCTATCGCGCGATATTGATTACATCACGCTGGCCTTGCAGCAGAGCTTCAGCGCGCA
ACAATTGGCAGGTGCAACGTTCCAGATCGCCAATGAGCTGTTTTACCGCAACAAGGCGGCCTGGCTGGTGGGGAAATTGC
GTGTGGCAGACCGGGTCTATCCTTTCCTGCTGCCGATCCATCACAGTGATTCCGGCGCCTTGTTTATTGATACCTGCCTG
ACCAGCAAGGCCGAGGCCAGCATCGTATTCGGCTTCGCCCGCTCCTACTTTATGGTTTACGCGCCCCTGCCGGCGGCAAT
GGTCGAGTGGCTGCGTGAAATACTGCCGGGCAAAACCACCGCTGAGCTGTATATGGCGATTGGTTGCCAGAAGCACGGAA
AAACAGAATGTTACCGTGAGTACCTGACCTTTATGAGTGGCTCTCAGGAGCAGTTCATCATCGCGCCGGGGGTGAAGGGC
ATGGTGATGCTGGTGTTTACCCTGCCGTCTTTCGATCGGGTATTCAAGGTGATCAAGGATGAGTTTGCGCCACAAAAAGA
GGTGACGCAGGCGCAGGTGATGGCCTGCTACCAACTGGTGAAAGAACACGATCGCGTAGGACGCATGGCGGATACCCAGG
AGTACGAAAACTTTGTCGTCGACAAGGCGCGACTCAGTCCGGAACTGCTGGCGGAACTGCAGCGCGAGGTGCCGGATAAG
CTGGAGGATCTGGGCGATCGCATCGCGATCAGGCATCTGTATATGGAACGACGCATGACACCGCTGAATCTGTATCTGGA
GCAGGCCGATGAACAGCAGATGCGCGACGCGATAGAAGAATACGGTAACGCCATCAAGCAACTGGCCGCCGCCAATATTT
TCCCCGGCGATATGCTGTTCAAGAACTTTGGCGTGACCCGGCACGGGCGGGTGGTGTTCTACGACTACGATGAGATTTGC
TACATGACCGAGGTCAACTTCCGCGATATTCCGCCACCGCGCTATCCGGAAGATGAGTTGGCCAGCGAACCCTGGTACAG
CGTGTCACCCAATGACGTCTTCCCGGAGGAATTCAGGCACTTTCTGTGTGGTGACCGGCGCATCCGGCAGGTGTTTGAAG
AGATGCATCGCGACCTGTTCGAGGCCGACTACTGGCGCGGGTTGCAACAGCGCATCCGCGATGGGCATGTGGAAGACGTG
TTTGCTTACCGCAAAAAACGGCGCTTCAGCCAGCGTAGTGGTGCGGCGCTGCCCGCGACATCGGCTACGGCATAA

Upstream 100 bases:

>100_bases
ACCGGTTCTACGGAAGAACAGCAGTTCTGATGCAGTCACGCTGACGATAGTTAACTCTGAGCCTGCTTATGCAGGCTCTC
TTTTCTGGCGGGAGGTAAGG

Downstream 100 bases:

>100_bases
GGGCGCGGTTCATTACCGCGCCCGGCAAGATTACGCTTTTGCACCCGCAACGGCGGCGCGCGCCAATTCGGTAATGCGGG
CGTAGTCACCGCTTTCCAGT

Product: bifunctional isocitrate dehydrogenase kinase/phosphatase protein

Products: NA

Alternate protein names: IDH kinase/phosphatase; IDHK/P

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MAAKLELLIAQTILQGFDAQYGRFLEVTAGAQQRFERADWPAVQQAMKKRIHLYDHHVGLVVEQLKCITGQQYFDADFPS
RVKAVYTDLLPEYPRFEIAESFFNSVYCRLFRHRDLTPEKLFVFSSQPEGRFRDIPRPLSRDFTANGDVSAMLYNLLTDL
PLRLPWENLSRDIDYITLALQQSFSAQQLAGATFQIANELFYRNKAAWLVGKLRVADRVYPFLLPIHHSDSGALFIDTCL
TSKAEASIVFGFARSYFMVYAPLPAAMVEWLREILPGKTTAELYMAIGCQKHGKTECYREYLTFMSGSQEQFIIAPGVKG
MVMLVFTLPSFDRVFKVIKDEFAPQKEVTQAQVMACYQLVKEHDRVGRMADTQEYENFVVDKARLSPELLAELQREVPDK
LEDLGDRIAIRHLYMERRMTPLNLYLEQADEQQMRDAIEEYGNAIKQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
YMTEVNFRDIPPPRYPEDELASEPWYSVSPNDVFPEEFRHFLCGDRRIRQVFEEMHRDLFEADYWRGLQQRIRDGHVEDV
FAYRKKRRFSQRSGAALPATSATA

Sequences:

>Translated_584_residues
MAAKLELLIAQTILQGFDAQYGRFLEVTAGAQQRFERADWPAVQQAMKKRIHLYDHHVGLVVEQLKCITGQQYFDADFPS
RVKAVYTDLLPEYPRFEIAESFFNSVYCRLFRHRDLTPEKLFVFSSQPEGRFRDIPRPLSRDFTANGDVSAMLYNLLTDL
PLRLPWENLSRDIDYITLALQQSFSAQQLAGATFQIANELFYRNKAAWLVGKLRVADRVYPFLLPIHHSDSGALFIDTCL
TSKAEASIVFGFARSYFMVYAPLPAAMVEWLREILPGKTTAELYMAIGCQKHGKTECYREYLTFMSGSQEQFIIAPGVKG
MVMLVFTLPSFDRVFKVIKDEFAPQKEVTQAQVMACYQLVKEHDRVGRMADTQEYENFVVDKARLSPELLAELQREVPDK
LEDLGDRIAIRHLYMERRMTPLNLYLEQADEQQMRDAIEEYGNAIKQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
YMTEVNFRDIPPPRYPEDELASEPWYSVSPNDVFPEEFRHFLCGDRRIRQVFEEMHRDLFEADYWRGLQQRIRDGHVEDV
FAYRKKRRFSQRSGAALPATSATA
>Mature_583_residues
AAKLELLIAQTILQGFDAQYGRFLEVTAGAQQRFERADWPAVQQAMKKRIHLYDHHVGLVVEQLKCITGQQYFDADFPSR
VKAVYTDLLPEYPRFEIAESFFNSVYCRLFRHRDLTPEKLFVFSSQPEGRFRDIPRPLSRDFTANGDVSAMLYNLLTDLP
LRLPWENLSRDIDYITLALQQSFSAQQLAGATFQIANELFYRNKAAWLVGKLRVADRVYPFLLPIHHSDSGALFIDTCLT
SKAEASIVFGFARSYFMVYAPLPAAMVEWLREILPGKTTAELYMAIGCQKHGKTECYREYLTFMSGSQEQFIIAPGVKGM
VMLVFTLPSFDRVFKVIKDEFAPQKEVTQAQVMACYQLVKEHDRVGRMADTQEYENFVVDKARLSPELLAELQREVPDKL
EDLGDRIAIRHLYMERRMTPLNLYLEQADEQQMRDAIEEYGNAIKQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEICY
MTEVNFRDIPPPRYPEDELASEPWYSVSPNDVFPEEFRHFLCGDRRIRQVFEEMHRDLFEADYWRGLQQRIRDGHVEDVF
AYRKKRRFSQRSGAALPATSATA

Specific function: Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source

COG id: COG4579

COG function: function code T; Isocitrate dehydrogenase kinase/phosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AceK family

Homologues:

Organism=Escherichia coli, GI1790446, Length=577, Percent_Identity=75.3899480069324, Blast_Score=911, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ACEK_SERP5 (A8GKF5)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480723.1
- STRING:   A8GKF5
- GeneID:   5607461
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4501
- eggNOG:   COG4579
- HOGENOM:   HBG298593
- OMA:   RMTPLNI
- ProtClustDB:   PRK02946
- BioCyc:   SPRO399741:SPRO_4501-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00747
- InterPro:   IPR010452
- PIRSF:   PIRSF000719
- ProDom:   PD043552

Pfam domain/function: PF06315 AceK

EC number: =2.7.11.5

Molecular weight: Translated: 67748; Mature: 67617

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: ACT_SITE 371-371 BINDING 336-336

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKLELLIAQTILQGFDAQYGRFLEVTAGAQQRFERADWPAVQQAMKKRIHLYDHHVGL
CCCHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VVEQLKCITGQQYFDADFPSRVKAVYTDLLPEYPRFEIAESFFNSVYCRLFRHRDLTPEK
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
LFVFSSQPEGRFRDIPRPLSRDFTANGDVSAMLYNLLTDLPLRLPWENLSRDIDYITLAL
EEEECCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHH
QQSFSAQQLAGATFQIANELFYRNKAAWLVGKLRVADRVYPFLLPIHHSDSGALFIDTCL
HHCCCHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEEEHH
TSKAEASIVFGFARSYFMVYAPLPAAMVEWLREILPGKTTAELYMAIGCQKHGKTECYRE
CCCCCHHHHHHHHHHHEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHH
YLTFMSGSQEQFIIAPGVKGMVMLVFTLPSFDRVFKVIKDEFAPQKEVTQAQVMACYQLV
HHHHHCCCCCCEEECCCCCCEEEHEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KEHDRVGRMADTQEYENFVVDKARLSPELLAELQREVPDKLEDLGDRIAIRHLYMERRMT
HHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLNLYLEQADEQQMRDAIEEYGNAIKQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCCEE
YMTEVNFRDIPPPRYPEDELASEPWYSVSPNDVFPEEFRHFLCGDRRIRQVFEEMHRDLF
EEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
EADYWRGLQQRIRDGHVEDVFAYRKKRRFSQRSGAALPATSATA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AAKLELLIAQTILQGFDAQYGRFLEVTAGAQQRFERADWPAVQQAMKKRIHLYDHHVGL
CCHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VVEQLKCITGQQYFDADFPSRVKAVYTDLLPEYPRFEIAESFFNSVYCRLFRHRDLTPEK
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
LFVFSSQPEGRFRDIPRPLSRDFTANGDVSAMLYNLLTDLPLRLPWENLSRDIDYITLAL
EEEECCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHH
QQSFSAQQLAGATFQIANELFYRNKAAWLVGKLRVADRVYPFLLPIHHSDSGALFIDTCL
HHCCCHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEEEHH
TSKAEASIVFGFARSYFMVYAPLPAAMVEWLREILPGKTTAELYMAIGCQKHGKTECYRE
CCCCCHHHHHHHHHHHEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHH
YLTFMSGSQEQFIIAPGVKGMVMLVFTLPSFDRVFKVIKDEFAPQKEVTQAQVMACYQLV
HHHHHCCCCCCEEECCCCCCEEEHEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KEHDRVGRMADTQEYENFVVDKARLSPELLAELQREVPDKLEDLGDRIAIRHLYMERRMT
HHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLNLYLEQADEQQMRDAIEEYGNAIKQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCCEE
YMTEVNFRDIPPPRYPEDELASEPWYSVSPNDVFPEEFRHFLCGDRRIRQVFEEMHRDLF
EEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
EADYWRGLQQRIRDGHVEDVFAYRKKRRFSQRSGAALPATSATA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA