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The map label for this gene is dnaB [H]

Identifier: 157372682

GI number: 157372682

Start: 4922148

End: 4923572

Strand: Reverse

Name: dnaB [H]

Synonym: Spro_4449

Alternate gene names: 157372682

Gene position: 4923572-4922148 (Counterclockwise)

Preceding gene: 157372685

Following gene: 157372681

Centisome position: 90.36

GC content: 56.63

Gene sequence:

>1425_bases
ATGGCAGGAAAAAAACCAACCAACAGAACAAACGCAGACGAAGCCAAAGAGCGCCCAAGAGACCGCCAGATGGAAGGGCT
GAAGATGCCGCCACATTCGCTGGAGGCGGAACAGTCCGTGTTGGGCGGTTTGATGCTGGATAACGAGCGCTGGGACAACG
TGTCCGAACGCGTAGTTTCCAACGATTTCTTCAGCCGCCCGCATCGCCTGATCTTTACCGAGATGCAGCGCCTGCTGGAA
ATGAGCCGACCGATCGACCTGATCACCCTGTCCGAATCGCTGGAACAGCGCGGTGAGCTCGATTCCGTCGGTGGGTTCGC
CTACCTGGCCGAATTATCGAAAAACACCCCAAGTGCTGCCAACATCGGTGCTTATGCAGATATCGTACGTGAACGTGCGG
TAGTGCGTGAGATGATCTCGGTGGCCAATGAAATCGCCGACGCCGGTTATGATCCGCAGGGTCGCAGCAGTGAAGACCTG
CTCGATCTGGCCGAATCACGGGTGTTCCAAATCGCGGAAAACCGGGCCAGCAAAGATGAAGGCCCGAAAGGCATTGAGCG
TATCCTGGAAGATACCGTTGCGCGCATAGAACAGCTGTATCAGCAGCCGCACGATGGCGTTACCGGGGTGGATACCGGCT
ATCAGGATCTCAATAAGAAAACCGCCGGTTTGCAAAAATCGGATCTGATCATCGTCGCCGCCCGTCCTTCGATGGGTAAA
ACTACCTTCGCCATGAACCTGTGCGAACACGCTGCGATGACGCAGGAAAAACCGGTGCTGATCTTCAGCCTTGAGATGCC
AGGCAACCAGATCATGATGCGTATGCTGGCGTCGCTTTCGCGCGTAGACCAGACGCGTATTCGTACCGGCCAGCTCGATG
ACGAGGACTGGGCGCGAATCTCCAGCACCATGGGTATCCTGCTGGAGAAGCGCAATATGTACATTGATGACTCCTCCGGC
CTGACGCCAACCGAAGTTCGCTCCCGCGCCCGCCGTATTTTCCGTGAACATGACGGCCTGAGCCTGATCATGATCGACTA
CCTGCAGCTGATGCGCGTGCCGTCCCTGTCCGACAACCGTACGCTGGAGATCGCTGAAATTTCCCGCTCGCTCAAGGCGT
TGGCGAAAGAACTGCAGGTGCCGGTGGTGGCGCTGTCGCAGTTGAACCGAAGCCTGGAGCAGCGTGCCGACAAGCGCCCG
GTCAACTCCGACCTGCGTGAGTCCGGTTCCATCGAGCAGGATGCCGACCTGATTATGTTCATCTACCGTGATGAGGTTTA
TCACGAGAACAGCGATCTGAAAGGGATTGCCGAAATTATTCTGGGTAAGCAGCGTAACGGCCCGATCGGTTCCGTGCGGC
TGACCTTTAACGGCCAGTGGTCGCGTTTCGATAACTACGCGGGGCCACAATATGATGACGAATAA

Upstream 100 bases:

>100_bases
GATACTTTTTGCATCTGTTATGCGCAGGAATCACAGCTAAAAATCACGTATACTCTCGCGTCAGGATCCTCCATTCGCCA
CCGGATAAAGAATACGTTTC

Downstream 100 bases:

>100_bases
TCAGCTAAGGAACTGAAATGAAAGCGGCAACCGCTGTAATCGACCGCCGCGCTCTGCGACATAATCTGCAACAGGTGCGC
CGTCAGGCGCCGCAAAGTCG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MAGKKPTNRTNADEAKERPRDRQMEGLKMPPHSLEAEQSVLGGLMLDNERWDNVSERVVSNDFFSRPHRLIFTEMQRLLE
MSRPIDLITLSESLEQRGELDSVGGFAYLAELSKNTPSAANIGAYADIVRERAVVREMISVANEIADAGYDPQGRSSEDL
LDLAESRVFQIAENRASKDEGPKGIERILEDTVARIEQLYQQPHDGVTGVDTGYQDLNKKTAGLQKSDLIIVAARPSMGK
TTFAMNLCEHAAMTQEKPVLIFSLEMPGNQIMMRMLASLSRVDQTRIRTGQLDDEDWARISSTMGILLEKRNMYIDDSSG
LTPTEVRSRARRIFREHDGLSLIMIDYLQLMRVPSLSDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRP
VNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIILGKQRNGPIGSVRLTFNGQWSRFDNYAGPQYDDE

Sequences:

>Translated_474_residues
MAGKKPTNRTNADEAKERPRDRQMEGLKMPPHSLEAEQSVLGGLMLDNERWDNVSERVVSNDFFSRPHRLIFTEMQRLLE
MSRPIDLITLSESLEQRGELDSVGGFAYLAELSKNTPSAANIGAYADIVRERAVVREMISVANEIADAGYDPQGRSSEDL
LDLAESRVFQIAENRASKDEGPKGIERILEDTVARIEQLYQQPHDGVTGVDTGYQDLNKKTAGLQKSDLIIVAARPSMGK
TTFAMNLCEHAAMTQEKPVLIFSLEMPGNQIMMRMLASLSRVDQTRIRTGQLDDEDWARISSTMGILLEKRNMYIDDSSG
LTPTEVRSRARRIFREHDGLSLIMIDYLQLMRVPSLSDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRP
VNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIILGKQRNGPIGSVRLTFNGQWSRFDNYAGPQYDDE
>Mature_473_residues
AGKKPTNRTNADEAKERPRDRQMEGLKMPPHSLEAEQSVLGGLMLDNERWDNVSERVVSNDFFSRPHRLIFTEMQRLLEM
SRPIDLITLSESLEQRGELDSVGGFAYLAELSKNTPSAANIGAYADIVRERAVVREMISVANEIADAGYDPQGRSSEDLL
DLAESRVFQIAENRASKDEGPKGIERILEDTVARIEQLYQQPHDGVTGVDTGYQDLNKKTAGLQKSDLIIVAARPSMGKT
TFAMNLCEHAAMTQEKPVLIFSLEMPGNQIMMRMLASLSRVDQTRIRTGQLDDEDWARISSTMGILLEKRNMYIDDSSGL
TPTEVRSRARRIFREHDGLSLIMIDYLQLMRVPSLSDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPV
NSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIILGKQRNGPIGSVRLTFNGQWSRFDNYAGPQYDDE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=474, Percent_Identity=85.8649789029536, Blast_Score=831, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 53437; Mature: 53306

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKKPTNRTNADEAKERPRDRQMEGLKMPPHSLEAEQSVLGGLMLDNERWDNVSERVVS
CCCCCCCCCCCCHHHHHCCHHHHHCCCCCCCCCCHHHHHHHCCHHCCCCHHCCHHHHHHH
NDFFSRPHRLIFTEMQRLLEMSRPIDLITLSESLEQRGELDSVGGFAYLAELSKNTPSAA
HHHHHCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCC
NIGAYADIVRERAVVREMISVANEIADAGYDPQGRSSEDLLDLAESRVFQIAENRASKDE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
GPKGIERILEDTVARIEQLYQQPHDGVTGVDTGYQDLNKKTAGLQKSDLIIVAARPSMGK
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCC
TTFAMNLCEHAAMTQEKPVLIFSLEMPGNQIMMRMLASLSRVDQTRIRTGQLDDEDWARI
HHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
SSTMGILLEKRNMYIDDSSGLTPTEVRSRARRIFREHDGLSLIMIDYLQLMRVPSLSDNR
HHHHHHHEECCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCC
TLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMF
EEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEE
IYRDEVYHENSDLKGIAEIILGKQRNGPIGSVRLTFNGQWSRFDNYAGPQYDDE
EEEHHHHCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AGKKPTNRTNADEAKERPRDRQMEGLKMPPHSLEAEQSVLGGLMLDNERWDNVSERVVS
CCCCCCCCCCCHHHHHCCHHHHHCCCCCCCCCCHHHHHHHCCHHCCCCHHCCHHHHHHH
NDFFSRPHRLIFTEMQRLLEMSRPIDLITLSESLEQRGELDSVGGFAYLAELSKNTPSAA
HHHHHCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCC
NIGAYADIVRERAVVREMISVANEIADAGYDPQGRSSEDLLDLAESRVFQIAENRASKDE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
GPKGIERILEDTVARIEQLYQQPHDGVTGVDTGYQDLNKKTAGLQKSDLIIVAARPSMGK
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCC
TTFAMNLCEHAAMTQEKPVLIFSLEMPGNQIMMRMLASLSRVDQTRIRTGQLDDEDWARI
HHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
SSTMGILLEKRNMYIDDSSGLTPTEVRSRARRIFREHDGLSLIMIDYLQLMRVPSLSDNR
HHHHHHHEECCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCC
TLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMF
EEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEE
IYRDEVYHENSDLKGIAEIILGKQRNGPIGSVRLTFNGQWSRFDNYAGPQYDDE
EEEHHHHCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]