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The map label for this gene is cca

Identifier: 157372526

GI number: 157372526

Start: 4760034

End: 4761278

Strand: Direct

Name: cca

Synonym: Spro_4293

Alternate gene names: 157372526

Gene position: 4760034-4761278 (Clockwise)

Preceding gene: 157372525

Following gene: 157372529

Centisome position: 87.36

GC content: 60.8

Gene sequence:

>1245_bases
GTGAAGACTTACCTGGTCGGCGGTGCCGTCCGCGACAGCTTGCTTGAGATACCGGTAGTCGACCGCGACTGGGTGGTGGT
AGGTGCTGCGCCTTCGGAGCTGACAGCCCTGGGCTATCAACAGGTCGGTAAAGACTTCCCGGTGTTTCTCAACCCCAAGA
CACATGAAGAATATGCGCTGGCGCGCACCGAGCGGAAATCCGGCCAGGGCTATACCGGTTTTACCTGCTACGCCGCGCCG
GACGTAACGCTGGAAGAAGATTTGCAACGGCGCGATTTAACCATCAACGCCATTGCCCGTAGCGACGACGGTGAGCTGAT
CGACCCTTTCAACGGCGTGGCCGACCTGCAAGCACGAGTACTGCGCCACGTGTCCGATGCCTTTGGTGAAGATCCGTTGC
GTGTACTGCGCGTGGCGCGTTTCGCCGCCCGCTTCGCCCATTTGGGTTTTAGCATCGCGCCGGAAACCGCCGAGTTGATG
CGTCAGATGGCACGCAGTGGTGAACTGGCAGCGCTGACGGCCGAGCGGGTATGGAAAGAAACCGAGAAGGCGCTGCAAAG
CCAGAGTCCACAGGTCTATTTTCAGGTACTGCGTGACTGCGAGGCGCTGGGCGTGCTGTTCCCGGAGATCGACACGCTGT
TCGGCGTACCGGCCCCCGCCAAATGGCACCCGGAGATCGACACCGGCATTCATACCCTGATGACGCTGGCTATCGCTGCC
CAGCTCAGCCCTGAAGTGGACGTACGCTTCTCCGCACTCTGTCACGACCTCGGCAAGGGGCAGACGCCAAAAGAGCTCTG
GCCGCATCACCACGGTCATGGACCGGCGGGCGTTCTGTTGGTCGAAGCCCTGTGCCGGCGGCTGCGAGTCCCCAATCCGG
TGCGCGATCTGGCCAAGCTGGTTGCCGAATATCACGATCTGATCCATACGGTAAACAAGCTGCGCCCTGAGACCTTGCTG
AAACTGTTTGATGCGGTCGATGTTTGGCGCAAACCGCAGCGGCTGGAACAGATGATCCTCACCAGCGAAGCCGACGCTCG
CGGCCGTACCGGCTTTGAAGACAACCCTTATCCGCAGGGCGACTATCTGCGCCAGGCGTTTCAGGTGGCGAATGCGGTAT
CGGTCAAAGAGGTGGTGGCCAGCGGCCTGCAAGGGCTGGCTGTCCGCGACGAGCTGAAACGCCGCCGCCAACAAGCGCTA
GCCGAGTGGAAACAAACTCAGGATATCCCTCTCGATCAGGCATAA

Upstream 100 bases:

>100_bases
TTGGGCGCCTGCGGGGCGCCCTTTTTATTGGTTTCGGCAGCGCCCCGCTAATTGAGGTAATCTGGTCAACAGAGCCTTAG
GATAAATTCAGGAGTTAACA

Downstream 100 bases:

>100_bases
AAAAACCCCGCAGCGCGGGGTTTCTTATTGCCACTCGGCTTACATGAAGACCATGTAAACCACGCCGGCCACGATAAAGC
GATAGATGGCAAACGGCACA

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MKTYLVGGAVRDSLLEIPVVDRDWVVVGAAPSELTALGYQQVGKDFPVFLNPKTHEEYALARTERKSGQGYTGFTCYAAP
DVTLEEDLQRRDLTINAIARSDDGELIDPFNGVADLQARVLRHVSDAFGEDPLRVLRVARFAARFAHLGFSIAPETAELM
RQMARSGELAALTAERVWKETEKALQSQSPQVYFQVLRDCEALGVLFPEIDTLFGVPAPAKWHPEIDTGIHTLMTLAIAA
QLSPEVDVRFSALCHDLGKGQTPKELWPHHHGHGPAGVLLVEALCRRLRVPNPVRDLAKLVAEYHDLIHTVNKLRPETLL
KLFDAVDVWRKPQRLEQMILTSEADARGRTGFEDNPYPQGDYLRQAFQVANAVSVKEVVASGLQGLAVRDELKRRRQQAL
AEWKQTQDIPLDQA

Sequences:

>Translated_414_residues
MKTYLVGGAVRDSLLEIPVVDRDWVVVGAAPSELTALGYQQVGKDFPVFLNPKTHEEYALARTERKSGQGYTGFTCYAAP
DVTLEEDLQRRDLTINAIARSDDGELIDPFNGVADLQARVLRHVSDAFGEDPLRVLRVARFAARFAHLGFSIAPETAELM
RQMARSGELAALTAERVWKETEKALQSQSPQVYFQVLRDCEALGVLFPEIDTLFGVPAPAKWHPEIDTGIHTLMTLAIAA
QLSPEVDVRFSALCHDLGKGQTPKELWPHHHGHGPAGVLLVEALCRRLRVPNPVRDLAKLVAEYHDLIHTVNKLRPETLL
KLFDAVDVWRKPQRLEQMILTSEADARGRTGFEDNPYPQGDYLRQAFQVANAVSVKEVVASGLQGLAVRDELKRRRQQAL
AEWKQTQDIPLDQA
>Mature_414_residues
MKTYLVGGAVRDSLLEIPVVDRDWVVVGAAPSELTALGYQQVGKDFPVFLNPKTHEEYALARTERKSGQGYTGFTCYAAP
DVTLEEDLQRRDLTINAIARSDDGELIDPFNGVADLQARVLRHVSDAFGEDPLRVLRVARFAARFAHLGFSIAPETAELM
RQMARSGELAALTAERVWKETEKALQSQSPQVYFQVLRDCEALGVLFPEIDTLFGVPAPAKWHPEIDTGIHTLMTLAIAA
QLSPEVDVRFSALCHDLGKGQTPKELWPHHHGHGPAGVLLVEALCRRLRVPNPVRDLAKLVAEYHDLIHTVNKLRPETLL
KLFDAVDVWRKPQRLEQMILTSEADARGRTGFEDNPYPQGDYLRQAFQVANAVSVKEVVASGLQGLAVRDELKRRRQQAL
AEWKQTQDIPLDQA

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=193, Percent_Identity=32.6424870466321, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1789436, Length=405, Percent_Identity=73.5802469135803, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI87081691, Length=231, Percent_Identity=32.9004329004329, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321016, Length=235, Percent_Identity=28.0851063829787, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_SERP5 (A8GJU7)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480515.1
- ProteinModelPortal:   A8GJU7
- SMR:   A8GJU7
- STRING:   A8GJU7
- GeneID:   5604463
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4293
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   GHGQKGL
- ProtClustDB:   PRK10885
- BioCyc:   SPRO399741:SPRO_4293-MONOMER
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 46185; Mature: 46185

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTYLVGGAVRDSLLEIPVVDRDWVVVGAAPSELTALGYQQVGKDFPVFLNPKTHEEYAL
CCEEEECHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEECCCCCHHHHH
ARTERKSGQGYTGFTCYAAPDVTLEEDLQRRDLTINAIARSDDGELIDPFNGVADLQARV
HHHHHHCCCCCCCEEEECCCCCCHHHHHHHHCCEEEEEEECCCCCEECCCCCHHHHHHHH
LRHVSDAFGEDPLRVLRVARFAARFAHLGFSIAPETAELMRQMARSGELAALTAERVWKE
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHH
TEKALQSQSPQVYFQVLRDCEALGVLFPEIDTLFGVPAPAKWHPEIDTGIHTLMTLAIAA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
QLSPEVDVRFSALCHDLGKGQTPKELWPHHHGHGPAGVLLVEALCRRLRVPNPVRDLAKL
HCCCCCCHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
VAEYHDLIHTVNKLRPETLLKLFDAVDVWRKPQRLEQMILTSEADARGRTGFEDNPYPQG
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
DYLRQAFQVANAVSVKEVVASGLQGLAVRDELKRRRQQALAEWKQTQDIPLDQA
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKTYLVGGAVRDSLLEIPVVDRDWVVVGAAPSELTALGYQQVGKDFPVFLNPKTHEEYAL
CCEEEECHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEECCCCCHHHHH
ARTERKSGQGYTGFTCYAAPDVTLEEDLQRRDLTINAIARSDDGELIDPFNGVADLQARV
HHHHHHCCCCCCCEEEECCCCCCHHHHHHHHCCEEEEEEECCCCCEECCCCCHHHHHHHH
LRHVSDAFGEDPLRVLRVARFAARFAHLGFSIAPETAELMRQMARSGELAALTAERVWKE
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHH
TEKALQSQSPQVYFQVLRDCEALGVLFPEIDTLFGVPAPAKWHPEIDTGIHTLMTLAIAA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
QLSPEVDVRFSALCHDLGKGQTPKELWPHHHGHGPAGVLLVEALCRRLRVPNPVRDLAKL
HCCCCCCHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
VAEYHDLIHTVNKLRPETLLKLFDAVDVWRKPQRLEQMILTSEADARGRTGFEDNPYPQG
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
DYLRQAFQVANAVSVKEVVASGLQGLAVRDELKRRRQQALAEWKQTQDIPLDQA
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA