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The map label for this gene is tolC [H]

Identifier: 157372501

GI number: 157372501

Start: 4731421

End: 4732914

Strand: Direct

Name: tolC [H]

Synonym: Spro_4268

Alternate gene names: 157372501

Gene position: 4731421-4732914 (Clockwise)

Preceding gene: 157372494

Following gene: 157372502

Centisome position: 86.83

GC content: 57.1

Gene sequence:

>1494_bases
ATGAAGAAACTGCTCCCCCTTCTTATCGGACTGAGCCTGGGCGGCTTCAGTGCAATGAGCCAGGCAGAGAACCTGCTGCA
GGTCTACAAACAGGCCAGGGAAAGTAACCCGGATCTGCGCAAAACCGCCGCTGACCGTGACGCCGCATTCGAAAAAATCA
ACGAAGCACGCAGCCCGTTGCTGCCGCAGTTGGGTTTGAGCGCCGGTTACACTTACACCAATGGCTACCGTGACAGCAAA
GATGCCAACAGCGATGCCACCAGTGGCTCCCTGGCGTTGACCCAGACTATCTTCGACATGTCCAAATGGCGTGCGCTGAC
GCTGCAGGAAAAAACCGCCGGCATTTCCGACGTGACTTTCCAAACCTCGTCACAGCAGCTGATCCTCGATACCGCTACCG
CCTATTTTAACGTGCTGAGCGCCATCGATACGCTGTCCTACACCCAGGCGAACAAGCAAGCGGTTTACCGCACCCTGGAC
CAGACCACCCAACGCTTTAACGTGGGCCTGGTCGCGATCACCGACGTGCAAAACGCCCGTTCGTCCTACGATACCGTGCT
GGCGGCCGAAGTCACCGCCCGTAACAACCTGGACAACGCGCTGGAAAAACTGCGCCAGGTCACCGGCACCTTCTATCCGG
AACTGGCCTCGTTGAATACCGACCGTTTCAACACCAAACGCCCGGATGCAGTCAATAATCTGCTGAAAGAAGCCGAAAGC
CGTAACCTGAGCCTGTTGTCCGCTCGCCTGAGCCAGGATCTGGCCCGTGAGCAGATCCGTTCCGCACAGACCGGTTATAT
GCCTACCGTTGATTTCAGCGCATCCACTGCGGTGAGCAATACTAATTACAGCGGTTCTCGCAACGTGAACAACGACGCTG
ATATTGGTCAGAACAAAGTGGGCCTGAGCTTTAACTTGCCGTTGTACAGCGGCGGCCAGACCAACTCACAGGTGCAGCAG
GCGCAGTACAACTTCGTTGGCGCCAGTGAGCAACTGGAAAGCGCCCACCGCAGCGTAGTGCAGACCGTGCGTTCTTCGTT
CAATAACGTGAATGCCTCGATCAGCAGCATCAACGCCTACCAACAAGCGGTAGTGTCTGCCCAGAGTTCATTGGATGCGA
CCGAGGCCGGTTACCAGGTAGGTACCCGTACCATCGTCGACGTGCTGGATGCGACCAGTACGCTGTATAACGCCAAGCAA
CAGCTCTCCAGCGCGCGTTATACCTACCTGATCAACCAACTGAACATCAAGTCGGCGCTCGGTACCCTGAACGAGAACGA
TCTGATGATGCTGAATGGCGCATTGGGTAAACCGATTTCTACTTCGCAAGACGTGGTAGCGCCACCGACTACCGCACAGG
ACGCTTACGCTGAAGGCTATAACGGCAACGCCCCTGCGCCACAAACTGCAGCACCGGTTGCCACCCGCGCCTCCGCACCG
GCGGCCACCACCAGCCAGCCTGCACGCACCAGCGGTAATCCATTCCGTAATTGA

Upstream 100 bases:

>100_bases
TTCAGCCACCTTAGGCGTACAACAGACTCTGCTAGAATCGGCAACTATTTCGTCTATCGTCAGCTAACTCCGCAATATTG
CTGCACAACAAGGAATGCAA

Downstream 100 bases:

>100_bases
TTGCCGTACTGCGCTGAATACGGGGTCCGGCATCTGCCGCACCCCGTTTTTTTTGCCTGCTCATCGGTTCTGCCCACCGC
CTCACAGCCGTTCCCCCACC

Product: outer membrane channel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MKKLLPLLIGLSLGGFSAMSQAENLLQVYKQARESNPDLRKTAADRDAAFEKINEARSPLLPQLGLSAGYTYTNGYRDSK
DANSDATSGSLALTQTIFDMSKWRALTLQEKTAGISDVTFQTSSQQLILDTATAYFNVLSAIDTLSYTQANKQAVYRTLD
QTTQRFNVGLVAITDVQNARSSYDTVLAAEVTARNNLDNALEKLRQVTGTFYPELASLNTDRFNTKRPDAVNNLLKEAES
RNLSLLSARLSQDLAREQIRSAQTGYMPTVDFSASTAVSNTNYSGSRNVNNDADIGQNKVGLSFNLPLYSGGQTNSQVQQ
AQYNFVGASEQLESAHRSVVQTVRSSFNNVNASISSINAYQQAVVSAQSSLDATEAGYQVGTRTIVDVLDATSTLYNAKQ
QLSSARYTYLINQLNIKSALGTLNENDLMMLNGALGKPISTSQDVVAPPTTAQDAYAEGYNGNAPAPQTAAPVATRASAP
AATTSQPARTSGNPFRN

Sequences:

>Translated_497_residues
MKKLLPLLIGLSLGGFSAMSQAENLLQVYKQARESNPDLRKTAADRDAAFEKINEARSPLLPQLGLSAGYTYTNGYRDSK
DANSDATSGSLALTQTIFDMSKWRALTLQEKTAGISDVTFQTSSQQLILDTATAYFNVLSAIDTLSYTQANKQAVYRTLD
QTTQRFNVGLVAITDVQNARSSYDTVLAAEVTARNNLDNALEKLRQVTGTFYPELASLNTDRFNTKRPDAVNNLLKEAES
RNLSLLSARLSQDLAREQIRSAQTGYMPTVDFSASTAVSNTNYSGSRNVNNDADIGQNKVGLSFNLPLYSGGQTNSQVQQ
AQYNFVGASEQLESAHRSVVQTVRSSFNNVNASISSINAYQQAVVSAQSSLDATEAGYQVGTRTIVDVLDATSTLYNAKQ
QLSSARYTYLINQLNIKSALGTLNENDLMMLNGALGKPISTSQDVVAPPTTAQDAYAEGYNGNAPAPQTAAPVATRASAP
AATTSQPARTSGNPFRN
>Mature_497_residues
MKKLLPLLIGLSLGGFSAMSQAENLLQVYKQARESNPDLRKTAADRDAAFEKINEARSPLLPQLGLSAGYTYTNGYRDSK
DANSDATSGSLALTQTIFDMSKWRALTLQEKTAGISDVTFQTSSQQLILDTATAYFNVLSAIDTLSYTQANKQAVYRTLD
QTTQRFNVGLVAITDVQNARSSYDTVLAAEVTARNNLDNALEKLRQVTGTFYPELASLNTDRFNTKRPDAVNNLLKEAES
RNLSLLSARLSQDLAREQIRSAQTGYMPTVDFSASTAVSNTNYSGSRNVNNDADIGQNKVGLSFNLPLYSGGQTNSQVQQ
AQYNFVGASEQLESAHRSVVQTVRSSFNNVNASISSINAYQQAVVSAQSSLDATEAGYQVGTRTIVDVLDATSTLYNAKQ
QLSSARYTYLINQLNIKSALGTLNENDLMMLNGALGKPISTSQDVVAPPTTAQDAYAEGYNGNAPAPQTAAPVATRASAP
AATTSQPARTSGNPFRN

Specific function: Required for proper expression of outer membrane protein genes such as ompF, nmpC, protein 2, hemolysin, colicin V, or colicin E1. May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=503, Percent_Identity=73.558648111332, Blast_Score=714, Evalue=0.0,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53530; Mature: 53530

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLPLLIGLSLGGFSAMSQAENLLQVYKQARESNPDLRKTAADRDAAFEKINEARSPL
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
LPQLGLSAGYTYTNGYRDSKDANSDATSGSLALTQTIFDMSKWRALTLQEKTAGISDVTF
CCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEHHHHCCCCCEEE
QTSSQQLILDTATAYFNVLSAIDTLSYTQANKQAVYRTLDQTTQRFNVGLVAITDVQNAR
ECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHH
SSYDTVLAAEVTARNNLDNALEKLRQVTGTFYPELASLNTDRFNTKRPDAVNNLLKEAES
HHHHHEEEEHHHHHCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHC
RNLSLLSARLSQDLAREQIRSAQTGYMPTVDFSASTAVSNTNYSGSRNVNNDADIGQNKV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GLSFNLPLYSGGQTNSQVQQAQYNFVGASEQLESAHRSVVQTVRSSFNNVNASISSINAY
CEEECCCEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QQAVVSAQSSLDATEAGYQVGTRTIVDVLDATSTLYNAKQQLSSARYTYLINQLNIKSAL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTLNENDLMMLNGALGKPISTSQDVVAPPTTAQDAYAEGYNGNAPAPQTAAPVATRASAP
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHCCCCC
AATTSQPARTSGNPFRN
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKLLPLLIGLSLGGFSAMSQAENLLQVYKQARESNPDLRKTAADRDAAFEKINEARSPL
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
LPQLGLSAGYTYTNGYRDSKDANSDATSGSLALTQTIFDMSKWRALTLQEKTAGISDVTF
CCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEHHHHCCCCCEEE
QTSSQQLILDTATAYFNVLSAIDTLSYTQANKQAVYRTLDQTTQRFNVGLVAITDVQNAR
ECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHH
SSYDTVLAAEVTARNNLDNALEKLRQVTGTFYPELASLNTDRFNTKRPDAVNNLLKEAES
HHHHHEEEEHHHHHCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHC
RNLSLLSARLSQDLAREQIRSAQTGYMPTVDFSASTAVSNTNYSGSRNVNNDADIGQNKV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GLSFNLPLYSGGQTNSQVQQAQYNFVGASEQLESAHRSVVQTVRSSFNNVNASISSINAY
CEEECCCEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QQAVVSAQSSLDATEAGYQVGTRTIVDVLDATSTLYNAKQQLSSARYTYLINQLNIKSAL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTLNENDLMMLNGALGKPISTSQDVVAPPTTAQDAYAEGYNGNAPAPQTAAPVATRASAP
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHCCCCC
AATTSQPARTSGNPFRN
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8801424 [H]