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The map label for this gene is queF [H]

Identifier: 157372430

GI number: 157372430

Start: 4651208

End: 4652050

Strand: Direct

Name: queF [H]

Synonym: Spro_4197

Alternate gene names: 157372430

Gene position: 4651208-4652050 (Clockwise)

Preceding gene: 157372429

Following gene: 157372431

Centisome position: 85.36

GC content: 55.75

Gene sequence:

>843_bases
ATGCATATCAAAAAACAAGATAAAATCACCCATCTTGGCGCCAACTCCGATTACCCGGACCAGTATGCTCCCGAGTTGCT
CGAAGCCCTGCCGCGCGCACGCGGTCGCGATCTGATCGGCGTGGATGAACGCAAACTGCCATTCAGCGGCTTCGACCTGT
GGACCGCCTTCGAACTTTCCTGGCTCAACGCCAAAGGCAAACCGGTGGTGGGGATTGGTGAATTTACCCTGCCCCATTCG
TCCACCAACCTGATCGAGTCCAAATCATTCAAACTGTATCTCAACAGCTTTAACCAGACGCGCTTCGACAGCGTGGAACA
GGTCAGCGCCGCGATGCAAAAAGATCTGTCGCAGGCCGCCAATGGTCAGGTTGGGGTGAAGCTGTATCCGGGGTTGGACG
GTTATTCAGCGCAGATCGACGCCCTGCCGGGCATCAATATCGACGACCTGGATATCGCCGTGGATGACTTTGACTTCAAG
CCGGAATATCTGATTGGTGCCACCAGCGAACAGGCGGAAATAGTGACGGAGACGCTGTCGTCCAACCTGCTGAAATCCAA
CTGTCTGGTGACCAATCAGCCGGACTGGGGCAGCGTGGTGATCCACTATCAGGGCCGTAAAATCGATCGCGAGCGCCTGC
TGCGCTATCTGATCTCCTTCCGCCAGCATAATGAGTTCCACGAGCAGTGTGTCGAGCGTATTTTTAACGACATCAAACAA
AGCTGCCAGCCGGAGAAACTCAGCGTGTTCGCCCGCTATACCCGCCGCGGCGGTCTGGACATCAACCCGTTCCGCAGCGA
TTTTGAAACGGCGCCGACCCTGGGCCGTTTGATCAGGCAATAA

Upstream 100 bases:

>100_bases
ATCAAGGTGGTCTACGCCCTGGTCGGTGTTGGGCCGATTTACGCCGCCCGCAGCCTGTTTAACCGTTATATCAATACCGA
TCTTGCAAGGAGCAATGAAT

Downstream 100 bases:

>100_bases
CCGTTCCCTTGTCATGCCCCCGCCCGGGGGCATTATTTCTCCCCGTCATCTGTTTGTTATTTTTCGCCATTACCGTCGTT
AACAACTATCTTCAAAACAC

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MHIKKQDKITHLGANSDYPDQYAPELLEALPRARGRDLIGVDERKLPFSGFDLWTAFELSWLNAKGKPVVGIGEFTLPHS
STNLIESKSFKLYLNSFNQTRFDSVEQVSAAMQKDLSQAANGQVGVKLYPGLDGYSAQIDALPGINIDDLDIAVDDFDFK
PEYLIGATSEQAEIVTETLSSNLLKSNCLVTNQPDWGSVVIHYQGRKIDRERLLRYLISFRQHNEFHEQCVERIFNDIKQ
SCQPEKLSVFARYTRRGGLDINPFRSDFETAPTLGRLIRQ

Sequences:

>Translated_280_residues
MHIKKQDKITHLGANSDYPDQYAPELLEALPRARGRDLIGVDERKLPFSGFDLWTAFELSWLNAKGKPVVGIGEFTLPHS
STNLIESKSFKLYLNSFNQTRFDSVEQVSAAMQKDLSQAANGQVGVKLYPGLDGYSAQIDALPGINIDDLDIAVDDFDFK
PEYLIGATSEQAEIVTETLSSNLLKSNCLVTNQPDWGSVVIHYQGRKIDRERLLRYLISFRQHNEFHEQCVERIFNDIKQ
SCQPEKLSVFARYTRRGGLDINPFRSDFETAPTLGRLIRQ
>Mature_280_residues
MHIKKQDKITHLGANSDYPDQYAPELLEALPRARGRDLIGVDERKLPFSGFDLWTAFELSWLNAKGKPVVGIGEFTLPHS
STNLIESKSFKLYLNSFNQTRFDSVEQVSAAMQKDLSQAANGQVGVKLYPGLDGYSAQIDALPGINIDDLDIAVDDFDFK
PEYLIGATSEQAEIVTETLSSNLLKSNCLVTNQPDWGSVVIHYQGRKIDRERLLRYLISFRQHNEFHEQCVERIFNDIKQ
SCQPEKLSVFARYTRRGGLDINPFRSDFETAPTLGRLIRQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=270, Percent_Identity=58.1481481481482, Blast_Score=325, Evalue=3e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 31730; Mature: 31730

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIKKQDKITHLGANSDYPDQYAPELLEALPRARGRDLIGVDERKLPFSGFDLWTAFELS
CCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHEEEE
WLNAKGKPVVGIGEFTLPHSSTNLIESKSFKLYLNSFNQTRFDSVEQVSAAMQKDLSQAA
EECCCCCEEEEECCEECCCCCCCHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
NGQVGVKLYPGLDGYSAQIDALPGINIDDLDIAVDDFDFKPEYLIGATSEQAEIVTETLS
CCCEEEEEECCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCEEEECCCHHHHHHHHHHH
SNLLKSNCLVTNQPDWGSVVIHYQGRKIDRERLLRYLISFRQHNEFHEQCVERIFNDIKQ
HHHHHCCCEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
SCQPEKLSVFARYTRRGGLDINPFRSDFETAPTLGRLIRQ
HCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MHIKKQDKITHLGANSDYPDQYAPELLEALPRARGRDLIGVDERKLPFSGFDLWTAFELS
CCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHEEEE
WLNAKGKPVVGIGEFTLPHSSTNLIESKSFKLYLNSFNQTRFDSVEQVSAAMQKDLSQAA
EECCCCCEEEEECCEECCCCCCCHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
NGQVGVKLYPGLDGYSAQIDALPGINIDDLDIAVDDFDFKPEYLIGATSEQAEIVTETLS
CCCEEEEEECCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCEEEECCCHHHHHHHHHHH
SNLLKSNCLVTNQPDWGSVVIHYQGRKIDRERLLRYLISFRQHNEFHEQCVERIFNDIKQ
HHHHHCCCEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
SCQPEKLSVFARYTRRGGLDINPFRSDFETAPTLGRLIRQ
HCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]