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The map label for this gene is mutY [H]

Identifier: 157372277

GI number: 157372277

Start: 4479915

End: 4481060

Strand: Direct

Name: mutY [H]

Synonym: Spro_4043

Alternate gene names: 157372277

Gene position: 4479915-4481060 (Clockwise)

Preceding gene: 157372269

Following gene: 157372278

Centisome position: 82.22

GC content: 57.77

Gene sequence:

>1146_bases
ATGCTGCAATCGCGCTTCATTTTCTGCCGGATATCGAATCTGCTTATGATGCAAGCACAACAGTTCGCACAGGTGGTGCT
TGACTGGTACCAGCGTTACGGCCGTAAAACCCTGCCGTGGCAGCTTGATAAAACCGCCTATAAAGTATGGCTCTCTGAGG
TCATGTTGCAACAAACTCAGGTTGCCACCGTGATCCCTTACTTTGAACGCTTTATGGCACGTTTTCCCAACGTGCGTGCG
CTGGCAGAAGCGCCGCTGGACGAAGTGCTGCACCTGTGGACCGGCCTGGGTTACTACGCCCGTGCTCGCAACCTGCACAA
GGCCGCGCAGACTATTGTCGCACAGCACGGCGGCGAGTTCCCGACAACCTTTGAAGAAATCCACGCCCTGCCCGGCATTG
GCCGCTCAACGGCCGGTGCGGTACTGTCATTGGCACTCGGCCAGCATTACCCGATCCTCGACGGCAACGTGAAACGCGTG
TTGGCTCGCTGCTATGCAGTCGAAGGCTGGCCGGGCAAAAAAGAGGTCGAAAACCGGCTGTGGCAGATCAGCGAAGACGT
CACCCCGGCGCAGGGCGTCGGCCAGTTCAATCAGGCGATGATGGACCTGGGGGCGATGGTTTGCACCCGCTCCAAACCCA
AGTGCGAGCTGTGCCCGCTCAACCTCGGCTGCATCGCCTATGCCCATCACAGTTGGGCGAAATACCCCGGCAAAAAGCCC
AAGCAGACGCTGCCGGAAAAAACCGCCTACTTTTTATTGCTGCAACACGGCGAACGAGTCTGGCTGGAACAGCGCCCGGC
CGTCGGCTTATGGGGCGGCCTGTTCTGCTTCCCGCAGTTCGGCGAACGCGAAGAGATGGAACTCTGGCTGCAACAACGCG
GTCTGAACAACAATCGCCAACAGCAGTTGACCGCATTTCGTCATACTTTCAGTCATTTCCATCTCGATATCGTGCCGATA
TGGTTGGAAATGAACGACGCGGCGGCCAGCATGGATGAGGGCGCCGGTCTCTGGTATAACTTGGCGCAGCCGCCATCGGT
CGGGCTGGCAGCGCCGGTTGACCGCCTGTTACAACAGTTGGCAAAACAGTCCCCGCGCCAACAGGGTTTATTTGGCGATA
GAGCCATTGATGAGGAATTAGCATGA

Upstream 100 bases:

>100_bases
GAAACGCGCATTATGCAAAGATACATTGGTTTAGCAAGTGTTCATCTTGTGTACCTGCCGTGCGACGTCGGAAAGTTCCG
GTTTACAGCATCATGACTCT

Downstream 100 bases:

>100_bases
GCAGAACCATTTTTTGTACCTTCCTGCAGCGCGACGCCGAAGGGCAGGATTTCCAGCTTTATCCAGGGGAAGTCGGCAAA
CGCATCTATAACGAGATCTC

Product: adenine DNA glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MLQSRFIFCRISNLLMMQAQQFAQVVLDWYQRYGRKTLPWQLDKTAYKVWLSEVMLQQTQVATVIPYFERFMARFPNVRA
LAEAPLDEVLHLWTGLGYYARARNLHKAAQTIVAQHGGEFPTTFEEIHALPGIGRSTAGAVLSLALGQHYPILDGNVKRV
LARCYAVEGWPGKKEVENRLWQISEDVTPAQGVGQFNQAMMDLGAMVCTRSKPKCELCPLNLGCIAYAHHSWAKYPGKKP
KQTLPEKTAYFLLLQHGERVWLEQRPAVGLWGGLFCFPQFGEREEMELWLQQRGLNNNRQQQLTAFRHTFSHFHLDIVPI
WLEMNDAAASMDEGAGLWYNLAQPPSVGLAAPVDRLLQQLAKQSPRQQGLFGDRAIDEELA

Sequences:

>Translated_381_residues
MLQSRFIFCRISNLLMMQAQQFAQVVLDWYQRYGRKTLPWQLDKTAYKVWLSEVMLQQTQVATVIPYFERFMARFPNVRA
LAEAPLDEVLHLWTGLGYYARARNLHKAAQTIVAQHGGEFPTTFEEIHALPGIGRSTAGAVLSLALGQHYPILDGNVKRV
LARCYAVEGWPGKKEVENRLWQISEDVTPAQGVGQFNQAMMDLGAMVCTRSKPKCELCPLNLGCIAYAHHSWAKYPGKKP
KQTLPEKTAYFLLLQHGERVWLEQRPAVGLWGGLFCFPQFGEREEMELWLQQRGLNNNRQQQLTAFRHTFSHFHLDIVPI
WLEMNDAAASMDEGAGLWYNLAQPPSVGLAAPVDRLLQQLAKQSPRQQGLFGDRAIDEELA
>Mature_381_residues
MLQSRFIFCRISNLLMMQAQQFAQVVLDWYQRYGRKTLPWQLDKTAYKVWLSEVMLQQTQVATVIPYFERFMARFPNVRA
LAEAPLDEVLHLWTGLGYYARARNLHKAAQTIVAQHGGEFPTTFEEIHALPGIGRSTAGAVLSLALGQHYPILDGNVKRV
LARCYAVEGWPGKKEVENRLWQISEDVTPAQGVGQFNQAMMDLGAMVCTRSKPKCELCPLNLGCIAYAHHSWAKYPGKKP
KQTLPEKTAYFLLLQHGERVWLEQRPAVGLWGGLFCFPQFGEREEMELWLQQRGLNNNRQQQLTAFRHTFSHFHLDIVPI
WLEMNDAAASMDEGAGLWYNLAQPPSVGLAAPVDRLLQQLAKQSPRQQGLFGDRAIDEELA

Specific function: Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine:7,8-dihydro-8- oxoguanine (8-oxo-dGTP) [H]

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI6912520, Length=357, Percent_Identity=35.5742296918768, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI190358497, Length=357, Percent_Identity=35.5742296918768, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI115298648, Length=357, Percent_Identity=35.5742296918768, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI115298650, Length=357, Percent_Identity=35.5742296918768, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI115298654, Length=357, Percent_Identity=35.5742296918768, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI115298652, Length=357, Percent_Identity=35.5742296918768, Blast_Score=186, Evalue=2e-47,
Organism=Escherichia coli, GI1789331, Length=349, Percent_Identity=75.9312320916905, Blast_Score=565, Evalue=1e-162,
Organism=Escherichia coli, GI1787920, Length=129, Percent_Identity=31.7829457364341, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 43366; Mature: 43366

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQSRFIFCRISNLLMMQAQQFAQVVLDWYQRYGRKTLPWQLDKTAYKVWLSEVMLQQTQ
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
VATVIPYFERFMARFPNVRALAEAPLDEVLHLWTGLGYYARARNLHKAAQTIVAQHGGEF
HHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCC
PTTFEEIHALPGIGRSTAGAVLSLALGQHYPILDGNVKRVLARCYAVEGWPGKKEVENRL
CCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
WQISEDVTPAQGVGQFNQAMMDLGAMVCTRSKPKCELCPLNLGCIAYAHHSWAKYPGKKP
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCC
KQTLPEKTAYFLLLQHGERVWLEQRPAVGLWGGLFCFPQFGEREEMELWLQQRGLNNNRQ
HHHCCHHHHEEEEECCCCEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCHH
QQLTAFRHTFSHFHLDIVPIWLEMNDAAASMDEGAGLWYNLAQPPSVGLAAPVDRLLQQL
HHHHHHHHHHHHHEEEEEEEEEEECHHHHCCCCCCCCEEECCCCCCCCCHHHHHHHHHHH
AKQSPRQQGLFGDRAIDEELA
HHCCCHHCCCCCCHHHHHCCC
>Mature Secondary Structure
MLQSRFIFCRISNLLMMQAQQFAQVVLDWYQRYGRKTLPWQLDKTAYKVWLSEVMLQQTQ
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
VATVIPYFERFMARFPNVRALAEAPLDEVLHLWTGLGYYARARNLHKAAQTIVAQHGGEF
HHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCC
PTTFEEIHALPGIGRSTAGAVLSLALGQHYPILDGNVKRVLARCYAVEGWPGKKEVENRL
CCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
WQISEDVTPAQGVGQFNQAMMDLGAMVCTRSKPKCELCPLNLGCIAYAHHSWAKYPGKKP
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCC
KQTLPEKTAYFLLLQHGERVWLEQRPAVGLWGGLFCFPQFGEREEMELWLQQRGLNNNRQ
HHHCCHHHHEEEEECCCCEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCHH
QQLTAFRHTFSHFHLDIVPIWLEMNDAAASMDEGAGLWYNLAQPPSVGLAAPVDRLLQQL
HHHHHHHHHHHHHEEEEEEEEEEECHHHHCCCCCCCCEEECCCCCCCCCHHHHHHHHHHH
AKQSPRQQGLFGDRAIDEELA
HHCCCHHCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2197596; 2001994; 9278503; 9846876 [H]