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The map label for this gene is cueO [H]

Identifier: 157372233

GI number: 157372233

Start: 4433592

End: 4435211

Strand: Reverse

Name: cueO [H]

Synonym: Spro_3999

Alternate gene names: 157372233

Gene position: 4435211-4433592 (Counterclockwise)

Preceding gene: 157372241

Following gene: 157372232

Centisome position: 81.4

GC content: 58.64

Gene sequence:

>1620_bases
ATGTTACGCCGTGATTTTATCAAATTGACCGCTGCGCTGGGCGCGGCAAGTGCTTTACCCTTGTGGAGCAGGGGCGTGTG
GGCAGCCGAGCTGCCGGCGTTGCCGATCCCGCCGCTGCTGATGCCGGACGCTCAAGGGAACATAGCGCTGGCGCTGCAAA
CCGGCGAAATGAACTGGCTGCCGGGCAAGGCGACCCAAACCTGGGGCGTTAATGGGGCGTTATTGGGGCCTGCGGTGCGA
TTGCAGCGCGGCAAGCCGGTGACGGTGGATATTCGCAATGGCTTGCCGGAGGCCAGTACCGTTCACTGGCACGGTCTGGA
AATCCCCGGTGACGTTGATGGCGGCCCGCAGGCACTGATTCAGCCAGGTACCACGCGCAGGGTGCAGTTCAAGGTCGAAC
AGCCCGCAGCCACCTGCTGGTTCCATCCGCATACTCACGGCAAGACCGGCCAGCAAGTGATGATGGGTTTGGCGGGGCTG
GTTCTGCTGGAAGACGACGAGAGCAGCAAGTTGCCGCTGCCTAAAACCTGGGGGCAGGATGATATTCCGGTGATCCTGCA
GGACAAACGGTTGGGTAAAGATGCACAGATTGAATACCAGCTGGACGTAATGAGTGCCGCAGTAGGCTGGTTCGGTGACC
GCATGTTCACCAACGGTGCCCAGTATCCACGGCATGTGGCACCGCGCGGTTGGCTGCGTTTGCGTTTCCTCAACGGTTGT
AATGCGCGTTCACTGAATCTGGCAACCAGCGATAATCGGCCGCTCTACGTGATCGCCAGCGACGGCGGCTTTTTGGCCGA
GCCGGTCAAGCTGACCGAACTGTCGATGCTGATGGGCGAACGCTTTGAAGTGCTGGTGGATGCCTCCGACGGCAAGCCTT
TCGATATTGTTACGCTGCCGGTTAAACAGATGGGCATGACGCTGGCCCCGTTCGACCAGCCGCTGCCGGTATTGCATATT
CAGCCTTCGCTGGCGCAGGGTATCAAGAGCATGCCGGACAGCCTGGTCAAATTGCCCGCAATACCGGCGGTAAGCGGGAT
CCAGGAGCGCTGGTTGCAACTGATGATGAACCCGCAGTTGGATAAGCTGGGTATGCAGGCGTTGATGGACCGCTACGGCC
ATCAGGCCATGGCCGGCATGAGCATGGACCACGGCAGCATGCCTAAAGACGGTGGTGATATGGCCGGCATGAAAGGCATG
GACCATGGCAGTATGAAGGGGATGGACCACGGCAATATGAAAGGGATGGATCACGGCGCCAAGTCGTTCGACTTCAGCCA
TGCCAATATGATCAACGGTAAGGCCTTCGACATGACCAAGCCGATGTTTGAGGCCAAGCGCGGCAAATACGAAAAATGGA
CCATTTCGGGTGAGGGCGACATGATGCTGCATCCGTTCCATATTCACGGCACCCAGTTCCGCATCCTGTCGGAAAACGGC
AAACCTCCGGCGGCTCACCGCAGCGGCTGGAAAGACACGGTACGGGTCGAAGGTTGGCGCAGTGAAGTATTGGTGCGCTT
TGATCACCCAGCCAATAGTGATCATGCCTATATGGCCCACTGCCACCTGTTGGAACATGAAGATACCGGCATGATGCTGG
GCTTTACCGTCACCGACTGA

Upstream 100 bases:

>100_bases
GGTTTAGGCTGCGAACAGATCAATCTATACCCATACTCTTTCAAGTTGCAGTGCAGCTTGTGCCGCATTAAGTGCCGATC
ACTGAAAAAGGGAGTCAGCG

Downstream 100 bases:

>100_bases
TCTACCCCCATGGAAAACCGGGCGGCGCACTCGCGTCGCCAGCGGCGTCAGGCTATGCTAAACTCTGCGCCCTTCTTCCG
GCAACTGACCTGAAAACTAT

Product: multicopper oxidase

Products: NA

Alternate protein names: Copper efflux oxidase [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MLRRDFIKLTAALGAASALPLWSRGVWAAELPALPIPPLLMPDAQGNIALALQTGEMNWLPGKATQTWGVNGALLGPAVR
LQRGKPVTVDIRNGLPEASTVHWHGLEIPGDVDGGPQALIQPGTTRRVQFKVEQPAATCWFHPHTHGKTGQQVMMGLAGL
VLLEDDESSKLPLPKTWGQDDIPVILQDKRLGKDAQIEYQLDVMSAAVGWFGDRMFTNGAQYPRHVAPRGWLRLRFLNGC
NARSLNLATSDNRPLYVIASDGGFLAEPVKLTELSMLMGERFEVLVDASDGKPFDIVTLPVKQMGMTLAPFDQPLPVLHI
QPSLAQGIKSMPDSLVKLPAIPAVSGIQERWLQLMMNPQLDKLGMQALMDRYGHQAMAGMSMDHGSMPKDGGDMAGMKGM
DHGSMKGMDHGNMKGMDHGAKSFDFSHANMINGKAFDMTKPMFEAKRGKYEKWTISGEGDMMLHPFHIHGTQFRILSENG
KPPAAHRSGWKDTVRVEGWRSEVLVRFDHPANSDHAYMAHCHLLEHEDTGMMLGFTVTD

Sequences:

>Translated_539_residues
MLRRDFIKLTAALGAASALPLWSRGVWAAELPALPIPPLLMPDAQGNIALALQTGEMNWLPGKATQTWGVNGALLGPAVR
LQRGKPVTVDIRNGLPEASTVHWHGLEIPGDVDGGPQALIQPGTTRRVQFKVEQPAATCWFHPHTHGKTGQQVMMGLAGL
VLLEDDESSKLPLPKTWGQDDIPVILQDKRLGKDAQIEYQLDVMSAAVGWFGDRMFTNGAQYPRHVAPRGWLRLRFLNGC
NARSLNLATSDNRPLYVIASDGGFLAEPVKLTELSMLMGERFEVLVDASDGKPFDIVTLPVKQMGMTLAPFDQPLPVLHI
QPSLAQGIKSMPDSLVKLPAIPAVSGIQERWLQLMMNPQLDKLGMQALMDRYGHQAMAGMSMDHGSMPKDGGDMAGMKGM
DHGSMKGMDHGNMKGMDHGAKSFDFSHANMINGKAFDMTKPMFEAKRGKYEKWTISGEGDMMLHPFHIHGTQFRILSENG
KPPAAHRSGWKDTVRVEGWRSEVLVRFDHPANSDHAYMAHCHLLEHEDTGMMLGFTVTD
>Mature_539_residues
MLRRDFIKLTAALGAASALPLWSRGVWAAELPALPIPPLLMPDAQGNIALALQTGEMNWLPGKATQTWGVNGALLGPAVR
LQRGKPVTVDIRNGLPEASTVHWHGLEIPGDVDGGPQALIQPGTTRRVQFKVEQPAATCWFHPHTHGKTGQQVMMGLAGL
VLLEDDESSKLPLPKTWGQDDIPVILQDKRLGKDAQIEYQLDVMSAAVGWFGDRMFTNGAQYPRHVAPRGWLRLRFLNGC
NARSLNLATSDNRPLYVIASDGGFLAEPVKLTELSMLMGERFEVLVDASDGKPFDIVTLPVKQMGMTLAPFDQPLPVLHI
QPSLAQGIKSMPDSLVKLPAIPAVSGIQERWLQLMMNPQLDKLGMQALMDRYGHQAMAGMSMDHGSMPKDGGDMAGMKGM
DHGSMKGMDHGNMKGMDHGAKSFDFSHANMINGKAFDMTKPMFEAKRGKYEKWTISGEGDMMLHPFHIHGTQFRILSENG
KPPAAHRSGWKDTVRVEGWRSEVLVRFDHPANSDHAYMAHCHLLEHEDTGMMLGFTVTD

Specific function: Probably involved in periplasmic detoxification of copper by oxidizing Cu(+) to Cu(2+) and thus preventing its uptake into the cytoplasm. Possesses phenoloxidase and ferroxidase activities and might be involved in the production of polyphenolic compounds

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 plastocyanin-like domains [H]

Homologues:

Organism=Escherichia coli, GI1786314, Length=538, Percent_Identity=67.1003717472119, Blast_Score=737, Evalue=0.0,
Organism=Escherichia coli, GI1789394, Length=316, Percent_Identity=37.6582278481013, Blast_Score=209, Evalue=4e-55,
Organism=Drosophila melanogaster, GI24650186, Length=249, Percent_Identity=28.5140562248996, Blast_Score=74, Evalue=4e-13,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011706
- InterPro:   IPR011707
- InterPro:   IPR002355
- InterPro:   IPR008972
- InterPro:   IPR006311 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07731 Cu-oxidase_2; PF07732 Cu-oxidase_3 [H]

EC number: NA

Molecular weight: Translated: 59241; Mature: 59241

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00080 MULTICOPPER_OXIDASE2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
6.5 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRDFIKLTAALGAASALPLWSRGVWAAELPALPIPPLLMPDAQGNIALALQTGEMNWL
CCCHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
PGKATQTWGVNGALLGPAVRLQRGKPVTVDIRNGLPEASTVHWHGLEIPGDVDGGPQALI
CCCCCEECCCCCEEECCEEEECCCCEEEEECCCCCCCCCEEEEECEECCCCCCCCCCEEE
QPGTTRRVQFKVEQPAATCWFHPHTHGKTGQQVMMGLAGLVLLEDDESSKLPLPKTWGQD
CCCCCEEEEEEEECCCEEEEECCCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCCCC
DIPVILQDKRLGKDAQIEYQLDVMSAAVGWFGDRMFTNGAQYPRHVAPRGWLRLRFLNGC
CCCEEEECCCCCCCCEEEEEEEHHHHHHHHHCCHHCCCCCCCCCCCCCCCEEEEEEECCC
NARSLNLATSDNRPLYVIASDGGFLAEPVKLTELSMLMGERFEVLVDASDGKPFDIVTLP
CCCEEEEEECCCCEEEEEECCCCCEECCEEHHHHHHHHCCCEEEEEECCCCCCEEEEEEC
VKQMGMTLAPFDQPLPVLHIQPSLAQGIKSMPDSLVKLPAIPAVSGIQERWLQLMMNPQL
HHHHCCEECCCCCCCCEEEECHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHCCCCH
DKLGMQALMDRYGHQAMAGMSMDHGSMPKDGGDMAGMKGMDHGSMKGMDHGNMKGMDHGA
HHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KSFDFSHANMINGKAFDMTKPMFEAKRGKYEKWTISGEGDMMLHPFHIHGTQFRILSENG
CCCCCCCCCCCCCCEECCCCHHHHHHCCCCEEEEECCCCCEEEEEEEECCEEEEEEECCC
KPPAAHRSGWKDTVRVEGWRSEVLVRFDHPANSDHAYMAHCHLLEHEDTGMMLGFTVTD
CCCCHHCCCCCCCEEEECCCCEEEEEECCCCCCCCEEEEEEEEEEECCCCEEEEEEECC
>Mature Secondary Structure
MLRRDFIKLTAALGAASALPLWSRGVWAAELPALPIPPLLMPDAQGNIALALQTGEMNWL
CCCHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
PGKATQTWGVNGALLGPAVRLQRGKPVTVDIRNGLPEASTVHWHGLEIPGDVDGGPQALI
CCCCCEECCCCCEEECCEEEECCCCEEEEECCCCCCCCCEEEEECEECCCCCCCCCCEEE
QPGTTRRVQFKVEQPAATCWFHPHTHGKTGQQVMMGLAGLVLLEDDESSKLPLPKTWGQD
CCCCCEEEEEEEECCCEEEEECCCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCCCC
DIPVILQDKRLGKDAQIEYQLDVMSAAVGWFGDRMFTNGAQYPRHVAPRGWLRLRFLNGC
CCCEEEECCCCCCCCEEEEEEEHHHHHHHHHCCHHCCCCCCCCCCCCCCCEEEEEEECCC
NARSLNLATSDNRPLYVIASDGGFLAEPVKLTELSMLMGERFEVLVDASDGKPFDIVTLP
CCCEEEEEECCCCEEEEEECCCCCEECCEEHHHHHHHHCCCEEEEEECCCCCCEEEEEEC
VKQMGMTLAPFDQPLPVLHIQPSLAQGIKSMPDSLVKLPAIPAVSGIQERWLQLMMNPQL
HHHHCCEECCCCCCCCEEEECHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHCCCCH
DKLGMQALMDRYGHQAMAGMSMDHGSMPKDGGDMAGMKGMDHGSMKGMDHGNMKGMDHGA
HHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KSFDFSHANMINGKAFDMTKPMFEAKRGKYEKWTISGEGDMMLHPFHIHGTQFRILSENG
CCCCCCCCCCCCCCEECCCCHHHHHHCCCCEEEEECCCCCEEEEEEEECCEEEEEEECCC
KPPAAHRSGWKDTVRVEGWRSEVLVRFDHPANSDHAYMAHCHLLEHEDTGMMLGFTVTD
CCCCHHCCCCCCCEEEECCCCEEEEEECCCCCCCCEEEEEEEEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]