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The map label for this gene is thcD [H]

Identifier: 157372197

GI number: 157372197

Start: 4391868

End: 4393079

Strand: Reverse

Name: thcD [H]

Synonym: Spro_3963

Alternate gene names: 157372197

Gene position: 4393079-4391868 (Counterclockwise)

Preceding gene: 157372198

Following gene: 157372196

Centisome position: 80.62

GC content: 63.04

Gene sequence:

>1212_bases
ATGAGCCAGCCAGAATCGGCGGGCATCCTGATCATCGGCGGCGGGCAGGCCGGGGGTTGGGCAGCGAAAACGCTGCGTGA
CCGTGGCTACACCGGCAAGCTGACGGTGGTCAGTGACGAACCCTATGACTTTTATGAACGTCCACCGCTTTCCAAGGCGG
CGTTGCTGGACGGCAATGCGCCGCTCAGCCGGCTGTTCAGTGAGCAGGTGGTGGCCGGGTTGAACATCAACTGGTGTCGC
CCGTTGCGTGCCGAAGCCATTGATGCACAGCAACAAATTGTCACTCTCAGCGACGGCCGACGGCTGCAGTTTGATCAACT
GCTGATCGCCACCGGTGGGCGACCGCGTCTGCCGGACGCCCGTTGGGCCAGTCATCCGCGCGTTATGACGCTGCGCTCCT
GGGACGATGCGACCCGACTGCGCCAGGCGCTGCAGGGTTGCCGCCGGCTTGCCATCGTCGGCGGCGGTTGGATTGGCCTG
GAGATCGCCGCTTCGGCACGGCGTCTCGGGGCTGGGGTAACGGTGTTTGAGCGCCAGCCTGCATTGTGCATGCGCAGCGT
GGGTGCGGACGTCTCCCAGGCGTTGCTTGAACTGCATCAGCAGCAGGGCGTAACGGTGCATTGCGGCTGCGGTGACATCC
AACTCGAAGATCGTGGCGGCAGTGCCTGGATCGGCAGCGACAGCAGCGATCCTCAGGAGTTTGATCTGGTGGTGGTGGGG
ATTGGCGTGGAGTTGAATCTGGAACTGGCGCATAGCGCCGGGCTGAAGGTTGACGCCGGCATCGTGGTTGACGGCCAGGG
ACGCACCAGTCACCCGGCGATCTTCGCCGCCGGAGACGTAGCTCGCCACCCGACGCTGGGTCTGTGCCTGCAATCCTGGG
CCTATGCGCAGAATCAGGCGATCAGCACCGCCTGCGCGATGCTGGATGCCTTTGCCTCACCTTACGATGACGTGGCCTGG
CTTTGGTCGGATCAATACGACGCCAATATCCAGATCCTCGGTATCCCGACCGGCGGCGTGCATCACGTAGTGCGACGCAC
GCCGCAGTCACAGGTGTTCTTCACGCTGAATGCCGACCGGCAGTTGGTGCAGATGGTGGCGTTTAACGACGCACGCACCA
TCAAGCTGGGCAAACGCTGGCTGGCCAGTGGGCGAGTGTTGGAGCCACAGCAGTTGGCGGACGCGGAGTTTTCTTTGATG
GCGTTGAAATAA

Upstream 100 bases:

>100_bases
CACGCGAGCTGGAGCACTCACTGGTGTCGTTCACCGAGTGGGCAGTGGAAGGCGGTATCGACCACGATACACGTCGCCAG
CATAAAAAGCCGGAGGCGTT

Downstream 100 bases:

>100_bases
CGCCCGTAACCTCGGGAGCGTTTCATGACTACGCAAGATATTGACGCCCGCGCTGGACGGGTGGGGGAAACCGTTGCCGA
AAATCCGCAGCAGCGGGTGC

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSQPESAGILIIGGGQAGGWAAKTLRDRGYTGKLTVVSDEPYDFYERPPLSKAALLDGNAPLSRLFSEQVVAGLNINWCR
PLRAEAIDAQQQIVTLSDGRRLQFDQLLIATGGRPRLPDARWASHPRVMTLRSWDDATRLRQALQGCRRLAIVGGGWIGL
EIAASARRLGAGVTVFERQPALCMRSVGADVSQALLELHQQQGVTVHCGCGDIQLEDRGGSAWIGSDSSDPQEFDLVVVG
IGVELNLELAHSAGLKVDAGIVVDGQGRTSHPAIFAAGDVARHPTLGLCLQSWAYAQNQAISTACAMLDAFASPYDDVAW
LWSDQYDANIQILGIPTGGVHHVVRRTPQSQVFFTLNADRQLVQMVAFNDARTIKLGKRWLASGRVLEPQQLADAEFSLM
ALK

Sequences:

>Translated_403_residues
MSQPESAGILIIGGGQAGGWAAKTLRDRGYTGKLTVVSDEPYDFYERPPLSKAALLDGNAPLSRLFSEQVVAGLNINWCR
PLRAEAIDAQQQIVTLSDGRRLQFDQLLIATGGRPRLPDARWASHPRVMTLRSWDDATRLRQALQGCRRLAIVGGGWIGL
EIAASARRLGAGVTVFERQPALCMRSVGADVSQALLELHQQQGVTVHCGCGDIQLEDRGGSAWIGSDSSDPQEFDLVVVG
IGVELNLELAHSAGLKVDAGIVVDGQGRTSHPAIFAAGDVARHPTLGLCLQSWAYAQNQAISTACAMLDAFASPYDDVAW
LWSDQYDANIQILGIPTGGVHHVVRRTPQSQVFFTLNADRQLVQMVAFNDARTIKLGKRWLASGRVLEPQQLADAEFSLM
ALK
>Mature_402_residues
SQPESAGILIIGGGQAGGWAAKTLRDRGYTGKLTVVSDEPYDFYERPPLSKAALLDGNAPLSRLFSEQVVAGLNINWCRP
LRAEAIDAQQQIVTLSDGRRLQFDQLLIATGGRPRLPDARWASHPRVMTLRSWDDATRLRQALQGCRRLAIVGGGWIGLE
IAASARRLGAGVTVFERQPALCMRSVGADVSQALLELHQQQGVTVHCGCGDIQLEDRGGSAWIGSDSSDPQEFDLVVVGI
GVELNLELAHSAGLKVDAGIVVDGQGRTSHPAIFAAGDVARHPTLGLCLQSWAYAQNQAISTACAMLDAFASPYDDVAWL
WSDQYDANIQILGIPTGGVHHVVRRTPQSQVFFTLNADRQLVQMVAFNDARTIKLGKRWLASGRVLEPQQLADAEFSLMA
LK

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=403, Percent_Identity=24.8138957816377, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI65787454, Length=403, Percent_Identity=24.8138957816377, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI21389617, Length=397, Percent_Identity=24.9370277078086, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI4757732, Length=360, Percent_Identity=26.3888888888889, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI22202629, Length=360, Percent_Identity=26.3888888888889, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788892, Length=400, Percent_Identity=36, Blast_Score=184, Evalue=6e-48,
Organism=Escherichia coli, GI1789065, Length=277, Percent_Identity=28.8808664259928, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789765, Length=373, Percent_Identity=26.0053619302949, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789915, Length=220, Percent_Identity=28.6363636363636, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17559934, Length=292, Percent_Identity=26.7123287671233, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24639257, Length=285, Percent_Identity=27.0175438596491, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI281359715, Length=285, Percent_Identity=27.0175438596491, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI281359713, Length=285, Percent_Identity=27.0175438596491, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24639250, Length=285, Percent_Identity=27.0175438596491, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI18543267, Length=285, Percent_Identity=27.0175438596491, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24639252, Length=285, Percent_Identity=27.0175438596491, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24585130, Length=283, Percent_Identity=22.9681978798587, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 43581; Mature: 43450

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPESAGILIIGGGQAGGWAAKTLRDRGYTGKLTVVSDEPYDFYERPPLSKAALLDGNA
CCCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCCCHHCCCCCCCEEEECCCC
PLSRLFSEQVVAGLNINWCRPLRAEAIDAQQQIVTLSDGRRLQFDQLLIATGGRPRLPDA
HHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCEEEEECCCCEEEEEEEEEECCCCCCCCCC
RWASHPRVMTLRSWDDATRLRQALQGCRRLAIVGGGWIGLEIAASARRLGAGVTVFERQP
CCCCCCEEEEEECCCHHHHHHHHHHHHHEEEEECCCEEEEEEEHHHHHHCCCEEEEECCC
ALCMRSVGADVSQALLELHQQQGVTVHCGCGDIQLEDRGGSAWIGSDSSDPQEFDLVVVG
HHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCEEEECCCCEEEECCCCCCCCEEEEEEEE
IGVELNLELAHSAGLKVDAGIVVDGQGRTSHPAIFAAGDVARHPTLGLCLQSWAYAQNQA
ECEEEEEEEECCCCCEEECEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCHH
ISTACAMLDAFASPYDDVAWLWSDQYDANIQILGIPTGGVHHVVRRTPQSQVFFTLNADR
HHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHCCCCCEEEEEECCCH
QLVQMVAFNDARTIKLGKRWLASGRVLEPQQLADAEFSLMALK
HHHHHHHCCCCEEEEHHHHHHHCCCCCCHHHHCCCCEEEEEEC
>Mature Secondary Structure 
SQPESAGILIIGGGQAGGWAAKTLRDRGYTGKLTVVSDEPYDFYERPPLSKAALLDGNA
CCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCCCHHCCCCCCCEEEECCCC
PLSRLFSEQVVAGLNINWCRPLRAEAIDAQQQIVTLSDGRRLQFDQLLIATGGRPRLPDA
HHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCEEEEECCCCEEEEEEEEEECCCCCCCCCC
RWASHPRVMTLRSWDDATRLRQALQGCRRLAIVGGGWIGLEIAASARRLGAGVTVFERQP
CCCCCCEEEEEECCCHHHHHHHHHHHHHEEEEECCCEEEEEEEHHHHHHCCCEEEEECCC
ALCMRSVGADVSQALLELHQQQGVTVHCGCGDIQLEDRGGSAWIGSDSSDPQEFDLVVVG
HHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCEEEECCCCEEEECCCCCCCCEEEEEEEE
IGVELNLELAHSAGLKVDAGIVVDGQGRTSHPAIFAAGDVARHPTLGLCLQSWAYAQNQA
ECEEEEEEEECCCCCEEECEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCHH
ISTACAMLDAFASPYDDVAWLWSDQYDANIQILGIPTGGVHHVVRRTPQSQVFFTLNADR
HHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHCCCCCEEEEEECCCH
QLVQMVAFNDARTIKLGKRWLASGRVLEPQQLADAEFSLMALK
HHHHHHHCCCCEEEEHHHHHHHCCCCCCHHHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]