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The map label for this gene is emrB [H]

Identifier: 157371979

GI number: 157371979

Start: 4133293

End: 4134828

Strand: Direct

Name: emrB [H]

Synonym: Spro_3744

Alternate gene names: 157371979

Gene position: 4133293-4134828 (Clockwise)

Preceding gene: 157371978

Following gene: 157371981

Centisome position: 75.86

GC content: 59.44

Gene sequence:

>1536_bases
TTGGCACAGAAACCGCTTGAAGGTGCCCAGCTCGCCTGGATGACGGTCGCGCTCGCCATGGCGACCTTTATGCAGGTGCT
GGACTCCACCATTGCCAACGTGGCGATCCCGACCATCGCGGGTAACCTCGGGTCTTCCAACTCGCAGGGTACCTGGGTGA
TCACCTCGTTTGGCGTGGCGAACGCTATCTCGATCCCGATCACCGGCTGGCTGGCGAAGCGCGTCGGTGAAGTGCGGCTG
TTCCTTTGGTCCACCGCACTGTTCGTGCTCGCTTCCTGGCTGTGCGGCATCTCCAACAGCCTGGGCATGCTGATCTTCTT
CCGCGTGATCCAGGGCGTGGTCGCGGGGCCACTGATCCCGCTGTCGCAAAGTCTGTTGCTGAACAACTATCCGCCCGCCA
AGCGAGCGATGGCCCTGGCGCTATGGTCGATGACGGTGATCGTCGCGCCAATATTCGGGCCTATCCTCGGCGGTTATATC
AGTGATAACTATCACTGGGGCTGGATCTTCTTCATCAATATTCCAATTGGCGCCGTAGTGATCCTGGTGGCGATGTCGAC
GTTGAAAGGCAGGGAAACCAAAACCGAGATCAAACCGATCGACACCATCGGTCTGGTGCTGTTGATCGTCGGTATTGGTT
CGCTGCAGGTGATGCTCGATCAGGGCAAGGAGCTGGACTGGTTCAACTCCACCGAGATAATAGTGCTAACGGTGGTGGCG
GTGGTGGCGCTGGTGTTCCTGATCATCTGGGAACTGACGGATGACCACCCGGTGGTGGATCTGTCGCTGTTCAAGTCGCG
CAACTTTACCGTCGGCTGCCTGTGTATCAGCCTGGCCTATATGCTGTACTTCGGCGCCATCGTGCTATTGCCGCAGCTGC
TGCAGGAGGTGTATGGCTATACCGCCACCTGGGCGGGGCTGGCTTCGGCGCCAATCGGGTTGATACCGGTATTGCTGTCA
CCAATCATCGGCAAGTTCGGTAACCGGCTCGACATGCGACGGCTGGTGACCTTCAGCTTCATTATGTATGCCGTGTGCTT
TTACTGGCGCGCCTATACGTTTGAACCGGGAATGGACTTTGGCGCCTCGGCCTGGCCGCAGTTTGTCCAGGGCTTCGCCA
TTGCCTGCTTCTTTATGCCATTGACCACCATTACGCTGTCCGGCCTGCCGCCGGAACGTATGGCGGCGGCATCGAGCCTG
TCGAACTTTACCCGTACGCTGGCGGGGTCGATCGGGACGTCGATCACCACCACGCTGTGGACTCAGCGCGAGTCGCAACA
CCACTCGCAGCTGGCGGAGTTTGTGAATCCTTACAGCCCGCAGTCGCAGGACATGTACCGGCAACTGGAACAGATTGGCA
TGAGCAAGCAACAGGCGTCGGCCTATATCGCCAACGAGATCACCGCGCAGGGGCTGATTATCTCGGCTAACGAGATATTC
TGGCTGTCCGCTGGGGTGTTCCTGGTGCTGCTGGCGCTGGTCTGGGTGGCGAAACCGCCATTCAGCTCCGGCGGTGGGGG
CGGCGGCGCTCACTAA

Upstream 100 bases:

>100_bases
TGTATCAGACCGACGCGCTGGCGTTGAACCTGGCACCGGTTAACCAGATGATCGCCGACGTGATCCATGCGAATGCCGGC
TAAGCGCGTGGGAGGCTACC

Downstream 100 bases:

>100_bases
ACAGTAGAAAGCAAAACGGGCACCCCTGGGTGCCCGTTTTTTTATAAAATATTATGTATACCCTATGAATTTCAAGTTGT
AGCTAGGCGACCAACTCACT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MAQKPLEGAQLAWMTVALAMATFMQVLDSTIANVAIPTIAGNLGSSNSQGTWVITSFGVANAISIPITGWLAKRVGEVRL
FLWSTALFVLASWLCGISNSLGMLIFFRVIQGVVAGPLIPLSQSLLLNNYPPAKRAMALALWSMTVIVAPIFGPILGGYI
SDNYHWGWIFFINIPIGAVVILVAMSTLKGRETKTEIKPIDTIGLVLLIVGIGSLQVMLDQGKELDWFNSTEIIVLTVVA
VVALVFLIIWELTDDHPVVDLSLFKSRNFTVGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPIGLIPVLLS
PIIGKFGNRLDMRRLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFVQGFAIACFFMPLTTITLSGLPPERMAAASSL
SNFTRTLAGSIGTSITTTLWTQRESQHHSQLAEFVNPYSPQSQDMYRQLEQIGMSKQQASAYIANEITAQGLIISANEIF
WLSAGVFLVLLALVWVAKPPFSSGGGGGGAH

Sequences:

>Translated_511_residues
MAQKPLEGAQLAWMTVALAMATFMQVLDSTIANVAIPTIAGNLGSSNSQGTWVITSFGVANAISIPITGWLAKRVGEVRL
FLWSTALFVLASWLCGISNSLGMLIFFRVIQGVVAGPLIPLSQSLLLNNYPPAKRAMALALWSMTVIVAPIFGPILGGYI
SDNYHWGWIFFINIPIGAVVILVAMSTLKGRETKTEIKPIDTIGLVLLIVGIGSLQVMLDQGKELDWFNSTEIIVLTVVA
VVALVFLIIWELTDDHPVVDLSLFKSRNFTVGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPIGLIPVLLS
PIIGKFGNRLDMRRLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFVQGFAIACFFMPLTTITLSGLPPERMAAASSL
SNFTRTLAGSIGTSITTTLWTQRESQHHSQLAEFVNPYSPQSQDMYRQLEQIGMSKQQASAYIANEITAQGLIISANEIF
WLSAGVFLVLLALVWVAKPPFSSGGGGGGAH
>Mature_510_residues
AQKPLEGAQLAWMTVALAMATFMQVLDSTIANVAIPTIAGNLGSSNSQGTWVITSFGVANAISIPITGWLAKRVGEVRLF
LWSTALFVLASWLCGISNSLGMLIFFRVIQGVVAGPLIPLSQSLLLNNYPPAKRAMALALWSMTVIVAPIFGPILGGYIS
DNYHWGWIFFINIPIGAVVILVAMSTLKGRETKTEIKPIDTIGLVLLIVGIGSLQVMLDQGKELDWFNSTEIIVLTVVAV
VALVFLIIWELTDDHPVVDLSLFKSRNFTVGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPIGLIPVLLSP
IIGKFGNRLDMRRLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFVQGFAIACFFMPLTTITLSGLPPERMAAASSLS
NFTRTLAGSIGTSITTTLWTQRESQHHSQLAEFVNPYSPQSQDMYRQLEQIGMSKQQASAYIANEITAQGLIISANEIFW
LSAGVFLVLLALVWVAKPPFSSGGGGGGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=499, Percent_Identity=83.1663326653307, Blast_Score=852, Evalue=0.0,
Organism=Escherichia coli, GI1788710, Length=503, Percent_Identity=60.8349900596421, Blast_Score=627, Evalue=0.0,
Organism=Escherichia coli, GI1790195, Length=436, Percent_Identity=31.651376146789, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI1788392, Length=446, Percent_Identity=30.7174887892377, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87081983, Length=425, Percent_Identity=28.7058823529412, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6322958, Length=539, Percent_Identity=22.0779220779221, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321663, Length=152, Percent_Identity=30.2631578947368, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319770, Length=291, Percent_Identity=24.3986254295533, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=143, Percent_Identity=26.5734265734266, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55504; Mature: 55373

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQKPLEGAQLAWMTVALAMATFMQVLDSTIANVAIPTIAGNLGSSNSQGTWVITSFGVA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
NAISIPITGWLAKRVGEVRLFLWSTALFVLASWLCGISNSLGMLIFFRVIQGVVAGPLIP
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
LSQSLLLNNYPPAKRAMALALWSMTVIVAPIFGPILGGYISDNYHWGWIFFINIPIGAVV
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCEEEEEEEEECCHHHHH
ILVAMSTLKGRETKTEIKPIDTIGLVLLIVGIGSLQVMLDQGKELDWFNSTEIIVLTVVA
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHHHH
VVALVFLIIWELTDDHPVVDLSLFKSRNFTVGCLCISLAYMLYFGAIVLLPQLLQEVYGY
HHHHHHHHHHHCCCCCCEEEHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TATWAGLASAPIGLIPVLLSPIIGKFGNRLDMRRLVTFSFIMYAVCFYWRAYTFEPGMDF
HHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GASAWPQFVQGFAIACFFMPLTTITLSGLPPERMAAASSLSNFTRTLAGSIGTSITTTLW
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
TQRESQHHSQLAEFVNPYSPQSQDMYRQLEQIGMSKQQASAYIANEITAQGLIISANEIF
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEECCCEE
WLSAGVFLVLLALVWVAKPPFSSGGGGGGAH
EHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AQKPLEGAQLAWMTVALAMATFMQVLDSTIANVAIPTIAGNLGSSNSQGTWVITSFGVA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
NAISIPITGWLAKRVGEVRLFLWSTALFVLASWLCGISNSLGMLIFFRVIQGVVAGPLIP
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
LSQSLLLNNYPPAKRAMALALWSMTVIVAPIFGPILGGYISDNYHWGWIFFINIPIGAVV
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCEEEEEEEEECCHHHHH
ILVAMSTLKGRETKTEIKPIDTIGLVLLIVGIGSLQVMLDQGKELDWFNSTEIIVLTVVA
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHHHH
VVALVFLIIWELTDDHPVVDLSLFKSRNFTVGCLCISLAYMLYFGAIVLLPQLLQEVYGY
HHHHHHHHHHHCCCCCCEEEHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TATWAGLASAPIGLIPVLLSPIIGKFGNRLDMRRLVTFSFIMYAVCFYWRAYTFEPGMDF
HHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GASAWPQFVQGFAIACFFMPLTTITLSGLPPERMAAASSLSNFTRTLAGSIGTSITTTLW
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
TQRESQHHSQLAEFVNPYSPQSQDMYRQLEQIGMSKQQASAYIANEITAQGLIISANEIF
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEECCCEE
WLSAGVFLVLLALVWVAKPPFSSGGGGGGAH
EHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]