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The map label for this gene is emrA [H]

Identifier: 157371978

GI number: 157371978

Start: 4132103

End: 4133275

Strand: Direct

Name: emrA [H]

Synonym: Spro_3743

Alternate gene names: 157371978

Gene position: 4132103-4133275 (Clockwise)

Preceding gene: 157371977

Following gene: 157371979

Centisome position: 75.83

GC content: 58.4

Gene sequence:

>1173_bases
ATGAGCGCAAGCGCGGAGACTCAGACCCCGCCACAACCTGTCGGCAAAAAGAAGCAGCGCAAGTTTTGGCTGCTGTTGAT
GACGGTTATTTTCGTTGTTATTGGGGTGGCATATTTAGTGTATTGGTTCCTGGTGTTGCGTCATCATCAGGAAACGGACG
ACGCCTATGTGTCCGGTAACCAGGTGCAGATCATGGCCCAGGTGAACGGTAGCGTTAACAGCGTCAACTTCGACAATACC
GACTACGTGAAGCAGGGGGATGTGCTGCTCACGCTGGATCCGACCGATGCCGAACAGGCGCTTGAACGCGCCAAAACCGG
CCTGGCCAACAGCGTGCGTCAGACCCACCAGCTGATCATCAACAGCAAGCAGTATCAGGCCAACATTGCCCTGCGCAAGT
CTGACCTGAGCAAGGCGGAGAACGATCTGAAGCGCCGCGTGGTACTGGGCAGCCTTGACGCCATCGGCCGCGAAGAACTG
CAACATGCGCGTGATGCGGTCGACAGCGCCAAAGCGGCACTGGAAGTGGCAGTTCAGCAATACAACGCCAACCAGGCGAT
GGTGCTGAACACCCCGCTGGAGCAACAGCCGGCTATCCAACAGTCCGCTGCGCAAATGCGTGACGCCTGGCTGGCGCTGC
AACGTACCAAAATCATCAGCCCGATCACCGGCTATGTGTCCCGTCGCAGCGTACAGGTTGGCGCGCAAATCGCCGCCGGT
TCCCCGCTGATGGCGGTGGTGCCTGCCGATCACATCTGGGTGGACGCCAACTTCAAAGAAACCCAGATCGCCAATATGCG
CATCGGTCAGTCGGCCACCGTGGTCAGTGATATTTACGGTGACGACGTGGTGTACAGCGGCAAAGTGGTCGGTATCGATA
TGGGCACCGGCAGCGCCTTCTCTCTGCTGCCTGCGCAGAATGCTACCGGTAACTGGATCAAGGTGGTTCAGCGCCTGCCG
GTACGTATTGAACTGGATGCCAAGCAAATCGCCGATCATCCGCTGCGTATCGGTCTGTCGACGCTGGTGAAAGTGGACAC
CACCAACCTGGACGGCCGCGTGCTGTCTGACGTGGTGCGTGACAAGCCGTTGTATCAGACCGACGCGCTGGCGTTGAACC
TGGCACCGGTTAACCAGATGATCGCCGACGTGATCCATGCGAATGCCGGCTAA

Upstream 100 bases:

>100_bases
CAGGCGAAGATAGGACTGATCCGTGCGCTGGGCGGTGGCTATCAGGCCCCGGTCGCTGCGGATTCAAAAGCATAATAAAA
TAGAGAGCTTGGAGAACACC

Downstream 100 bases:

>100_bases
GCGCGTGGGAGGCTACCTTGGCACAGAAACCGCTTGAAGGTGCCCAGCTCGCCTGGATGACGGTCGCGCTCGCCATGGCG
ACCTTTATGCAGGTGCTGGA

Product: efflux pump membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSASAETQTPPQPVGKKKQRKFWLLLMTVIFVVIGVAYLVYWFLVLRHHQETDDAYVSGNQVQIMAQVNGSVNSVNFDNT
DYVKQGDVLLTLDPTDAEQALERAKTGLANSVRQTHQLIINSKQYQANIALRKSDLSKAENDLKRRVVLGSLDAIGREEL
QHARDAVDSAKAALEVAVQQYNANQAMVLNTPLEQQPAIQQSAAQMRDAWLALQRTKIISPITGYVSRRSVQVGAQIAAG
SPLMAVVPADHIWVDANFKETQIANMRIGQSATVVSDIYGDDVVYSGKVVGIDMGTGSAFSLLPAQNATGNWIKVVQRLP
VRIELDAKQIADHPLRIGLSTLVKVDTTNLDGRVLSDVVRDKPLYQTDALALNLAPVNQMIADVIHANAG

Sequences:

>Translated_390_residues
MSASAETQTPPQPVGKKKQRKFWLLLMTVIFVVIGVAYLVYWFLVLRHHQETDDAYVSGNQVQIMAQVNGSVNSVNFDNT
DYVKQGDVLLTLDPTDAEQALERAKTGLANSVRQTHQLIINSKQYQANIALRKSDLSKAENDLKRRVVLGSLDAIGREEL
QHARDAVDSAKAALEVAVQQYNANQAMVLNTPLEQQPAIQQSAAQMRDAWLALQRTKIISPITGYVSRRSVQVGAQIAAG
SPLMAVVPADHIWVDANFKETQIANMRIGQSATVVSDIYGDDVVYSGKVVGIDMGTGSAFSLLPAQNATGNWIKVVQRLP
VRIELDAKQIADHPLRIGLSTLVKVDTTNLDGRVLSDVVRDKPLYQTDALALNLAPVNQMIADVIHANAG
>Mature_389_residues
SASAETQTPPQPVGKKKQRKFWLLLMTVIFVVIGVAYLVYWFLVLRHHQETDDAYVSGNQVQIMAQVNGSVNSVNFDNTD
YVKQGDVLLTLDPTDAEQALERAKTGLANSVRQTHQLIINSKQYQANIALRKSDLSKAENDLKRRVVLGSLDAIGREELQ
HARDAVDSAKAALEVAVQQYNANQAMVLNTPLEQQPAIQQSAAQMRDAWLALQRTKIISPITGYVSRRSVQVGAQIAAGS
PLMAVVPADHIWVDANFKETQIANMRIGQSATVVSDIYGDDVVYSGKVVGIDMGTGSAFSLLPAQNATGNWIKVVQRLPV
RIELDAKQIADHPLRIGLSTLVKVDTTNLDGRVLSDVVRDKPLYQTDALALNLAPVNQMIADVIHANAG

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=390, Percent_Identity=70.2564102564102, Blast_Score=558, Evalue=1e-160,
Organism=Escherichia coli, GI1788711, Length=374, Percent_Identity=50, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI87081951, Length=308, Percent_Identity=28.2467532467532, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1790519, Length=311, Percent_Identity=27.0096463022508, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789637, Length=316, Percent_Identity=27.5316455696203, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42623; Mature: 42492

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASAETQTPPQPVGKKKQRKFWLLLMTVIFVVIGVAYLVYWFLVLRHHQETDDAYVSGN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCC
QVQIMAQVNGSVNSVNFDNTDYVKQGDVLLTLDPTDAEQALERAKTGLANSVRQTHQLII
EEEEEEEECCCEEEECCCCCCCEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
NSKQYQANIALRKSDLSKAENDLKRRVVLGSLDAIGREELQHARDAVDSAKAALEVAVQQ
CCCCEEEEEEEEHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
YNANQAMVLNTPLEQQPAIQQSAAQMRDAWLALQRTKIISPITGYVSRRSVQVGAQIAAG
HCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCEEECC
SPLMAVVPADHIWVDANFKETQIANMRIGQSATVVSDIYGDDVVYSGKVVGIDMGTGSAF
CCEEEEECCCEEEEECCCCCCEEEEEECCCCHHHHHHHCCCCEEECCEEEEEECCCCCCE
SLLPAQNATGNWIKVVQRLPVRIELDAKQIADHPLRIGLSTLVKVDTTNLDGRVLSDVVR
EEECCCCCCCHHHHHHHHCCCEEEECHHHHCCCCHHCCHHHEEEEECCCCCHHHHHHHHC
DKPLYQTDALALNLAPVNQMIADVIHANAG
CCCCCCCCCEEEEECHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SASAETQTPPQPVGKKKQRKFWLLLMTVIFVVIGVAYLVYWFLVLRHHQETDDAYVSGN
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCC
QVQIMAQVNGSVNSVNFDNTDYVKQGDVLLTLDPTDAEQALERAKTGLANSVRQTHQLII
EEEEEEEECCCEEEECCCCCCCEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
NSKQYQANIALRKSDLSKAENDLKRRVVLGSLDAIGREELQHARDAVDSAKAALEVAVQQ
CCCCEEEEEEEEHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
YNANQAMVLNTPLEQQPAIQQSAAQMRDAWLALQRTKIISPITGYVSRRSVQVGAQIAAG
HCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCEEECC
SPLMAVVPADHIWVDANFKETQIANMRIGQSATVVSDIYGDDVVYSGKVVGIDMGTGSAF
CCEEEEECCCEEEEECCCCCCEEEEEECCCCHHHHHHHCCCCEEECCEEEEEECCCCCCE
SLLPAQNATGNWIKVVQRLPVRIELDAKQIADHPLRIGLSTLVKVDTTNLDGRVLSDVVR
EEECCCCCCCHHHHHHHHCCCEEEECHHHHCCCCHHCCHHHEEEEECCCCCHHHHHHHHC
DKPLYQTDALALNLAPVNQMIADVIHANAG
CCCCCCCCCEEEEECHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]