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The map label for this gene is proW [H]

Identifier: 157371969

GI number: 157371969

Start: 4123265

End: 4124389

Strand: Direct

Name: proW [H]

Synonym: Spro_3734

Alternate gene names: 157371969

Gene position: 4123265-4124389 (Clockwise)

Preceding gene: 157371968

Following gene: 157371970

Centisome position: 75.67

GC content: 61.96

Gene sequence:

>1125_bases
ATGAGTGATACTACACAGGATCCATGGGCCACTGCGCCTGCCGACGCCGGTGCCAGTGCCGCACCGGCCACTGACGCTGC
AAATTCAGCGGCTCCCGCCGGCGATGCCTGGTCGAGCGCTCCGCCACCGGCAAGCCATGACGCCGCTAATCAGGCCGCTC
AGGGCGCCGACTGGCTGAATAGCGCACCGGCTCAGCCTGAGCATTTCAACCTGCTCGATCCGTTCCACAAGACCTGGGTA
CCGTTTGACTCCTGGGTCACCCACGGCATTGATTGGCTGGTGCTGCACTTTCGCCCGCTGTTCCAGGGGATCCGCGTACC
GGTCGACTTTATCCTCAGCGGTTTTCAACAACTGCTGCTGGGCATGCCGGCACCGATCGCCATTTTGCTGTTCTCGCTGA
TTGCCTGGCAACTCTCCAGCCTCGGTATGGGTGCCGCCACGCTGGTCTCACTGATTGCCATTGGCGCTATTGGTGCCTGG
TCGCAGGCCATGGTGACGCTGGCGCTGGTATTGACCGCCCTGTTCTTCTGTATCGTCATCGGCTTGCCGCTCGGCATCTG
GCTGGCGCGCAGCAAGCACGCCGGCAAGGTCATACGGCCGTTGCTTGACGCCATGCAAACCACGCCGGCCTTCGTCTATC
TGGTGCCGATCGTCATGCTGTTCGGTATCGGTAACGTGCCGGGGGTGGTGGTTACCATTATCTTCGCCCTGCCGCCCATC
GTGCGGTTGACCATTCTGGGTATTAAGCAGGTGCCGGAAGATCTGATCGAAGCCGCTGAATCCTTTGGCGCCAGCCCGCG
CCAACTGCTGTTTAAAGTGCAGTTGCCATTGGCGATGCCGACCATTATGGCCGGGGTAAACCAAACGCTGATGCTGGCAC
TGTCGATGGTGGTGATCGCCTCGATGATCGCCGTCGGCGGTCTGGGCCAGATGGTGCTGCGCGGTATCGGCCGCCTCGAT
ATGGGGCTGGCCGCCGTCGGCGGGGTCGGTATCGTGATCCTGGCGATTATTCTCGACCGTCTCACCCAGTCGCTGGGGCG
CGATCGCCGCAGTAAAGGCATGGGGCGTTGGTACGCACACGGCCCCATCGGCCTGGTCACCCGGCCCTTCATCAAAAAGC
CTTAA

Upstream 100 bases:

>100_bases
GCGCCTTGCGCGGTACCTGTGGTCTGCGAAGAAAATAACTATATCGGAATTATTTCCAAGGCCATGCTGCTGCAGGCCCT
GGATAAAGAGGGCCCAACGA

Downstream 100 bases:

>100_bases
TTGCCCTGCCCCCGGCCAGCGTCGGGGGCACCGATAAAATCGAAGGAACCACTATGCGCTCGACAGGAATTTGGGCTTTG
GCCCTGACTTCATTGCTTGG

Product: glycine betaine transporter membrane protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MSDTTQDPWATAPADAGASAAPATDAANSAAPAGDAWSSAPPPASHDAANQAAQGADWLNSAPAQPEHFNLLDPFHKTWV
PFDSWVTHGIDWLVLHFRPLFQGIRVPVDFILSGFQQLLLGMPAPIAILLFSLIAWQLSSLGMGAATLVSLIAIGAIGAW
SQAMVTLALVLTALFFCIVIGLPLGIWLARSKHAGKVIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPI
VRLTILGIKQVPEDLIEAAESFGASPRQLLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIGRLD
MGLAAVGGVGIVILAIILDRLTQSLGRDRRSKGMGRWYAHGPIGLVTRPFIKKP

Sequences:

>Translated_374_residues
MSDTTQDPWATAPADAGASAAPATDAANSAAPAGDAWSSAPPPASHDAANQAAQGADWLNSAPAQPEHFNLLDPFHKTWV
PFDSWVTHGIDWLVLHFRPLFQGIRVPVDFILSGFQQLLLGMPAPIAILLFSLIAWQLSSLGMGAATLVSLIAIGAIGAW
SQAMVTLALVLTALFFCIVIGLPLGIWLARSKHAGKVIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPI
VRLTILGIKQVPEDLIEAAESFGASPRQLLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIGRLD
MGLAAVGGVGIVILAIILDRLTQSLGRDRRSKGMGRWYAHGPIGLVTRPFIKKP
>Mature_373_residues
SDTTQDPWATAPADAGASAAPATDAANSAAPAGDAWSSAPPPASHDAANQAAQGADWLNSAPAQPEHFNLLDPFHKTWVP
FDSWVTHGIDWLVLHFRPLFQGIRVPVDFILSGFQQLLLGMPAPIAILLFSLIAWQLSSLGMGAATLVSLIAIGAIGAWS
QAMVTLALVLTALFFCIVIGLPLGIWLARSKHAGKVIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIV
RLTILGIKQVPEDLIEAAESFGASPRQLLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIGRLDM
GLAAVGGVGIVILAIILDRLTQSLGRDRRSKGMGRWYAHGPIGLVTRPFIKKP

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=319, Percent_Identity=85.5799373040752, Blast_Score=520, Evalue=1e-149,
Organism=Escherichia coli, GI1788451, Length=191, Percent_Identity=31.9371727748691, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1786564, Length=154, Percent_Identity=32.4675324675325, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788449, Length=175, Percent_Identity=29.7142857142857, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 39509; Mature: 39378

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTTQDPWATAPADAGASAAPATDAANSAAPAGDAWSSAPPPASHDAANQAAQGADWLN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHH
SAPAQPEHFNLLDPFHKTWVPFDSWVTHGIDWLVLHFRPLFQGIRVPVDFILSGFQQLLL
CCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GMPAPIAILLFSLIAWQLSSLGMGAATLVSLIAIGAIGAWSQAMVTLALVLTALFFCIVI
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLPLGIWLARSKHAGKVIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPI
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VRLTILGIKQVPEDLIEAAESFGASPRQLLFKVQLPLAMPTIMAGVNQTLMLALSMVVIA
HHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHH
SMIAVGGLGQMVLRGIGRLDMGLAAVGGVGIVILAIILDRLTQSLGRDRRSKGMGRWYAH
HHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEECC
GPIGLVTRPFIKKP
CCCHHHCCCCCCCC
>Mature Secondary Structure 
SDTTQDPWATAPADAGASAAPATDAANSAAPAGDAWSSAPPPASHDAANQAAQGADWLN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHH
SAPAQPEHFNLLDPFHKTWVPFDSWVTHGIDWLVLHFRPLFQGIRVPVDFILSGFQQLLL
CCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GMPAPIAILLFSLIAWQLSSLGMGAATLVSLIAIGAIGAWSQAMVTLALVLTALFFCIVI
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLPLGIWLARSKHAGKVIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPI
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VRLTILGIKQVPEDLIEAAESFGASPRQLLFKVQLPLAMPTIMAGVNQTLMLALSMVVIA
HHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHH
SMIAVGGLGQMVLRGIGRLDMGLAAVGGVGIVILAIILDRLTQSLGRDRRSKGMGRWYAH
HHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEECC
GPIGLVTRPFIKKP
CCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503 [H]