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The map label for this gene is nrdF [H]

Identifier: 157371959

GI number: 157371959

Start: 4113010

End: 4113981

Strand: Direct

Name: nrdF [H]

Synonym: Spro_3724

Alternate gene names: 157371959

Gene position: 4113010-4113981 (Clockwise)

Preceding gene: 157371958

Following gene: 157371963

Centisome position: 75.48

GC content: 57.2

Gene sequence:

>972_bases
ATGAATAGCATTAAACCGGCGCAACTGGTGCGCGCAGTCAACTGGAACATCATCGAAGACGACAAGGATCTGGAGGTGTG
GAACCGTCTGACCTCCAACTTCTGGCTGCCGGAGAAGGTGCCGCTGTCGAACGATATTCCCTCGTGGGCCACGCTGACAC
CGAAGGAACAGCAACTGACCATCCGGGTTTTCACCGGCCTGACGCTGCTCGATACCATTCAAAATACCGTCGGGGCACCC
GCGCTGATCGCCGATGCCATTACGCCGCACGAGGAGGCGGTGTACTCCAATATCAGCTTTATGGAGGCGGTACACGCCCG
CTCCTACAGCTCGATTTTCTCCACGCTGTGCCAAACGCCGGACGTCGACGACGCCTACCGCTGGAGCGAGGAAAACCGTG
CGCTGCAGAAAAAGGCCAGCATCATTTTGGCCCACTACCGCAGTGACGATCCGCTGATGAAGAAGGTCGCCAGCGTGTTC
CTTGAGTCGTTCCTGTTCTATTCGGGCTTTTACCTGCCGATGTACTGGTCGAGCCGCGCCAAACTGACCAACACCGCCGA
CCTGATCCGGCTGATCATCCGCGACGAGGCGGTACACGGCTATTACATTGGCTATAAATTCCAGAAAGGGTTGGAGAAGG
TCGATGCCGCCCGCCGGCAACAGGTGAAAAACTTCGCCTTCGATCTGCTGCAGGATCTGTATGACAACGAGGTGCGGTAC
ACCGAAGAGCTGTACGACGGGGTGGGTTGGACCGAGGACGTGAAAACCTTCCTGCATTATAACGCCAACAAGGCGCTGAT
GAATCTGGGGTACGAAGCGCTGTTCCCACCGTCGATGGCGGAGGTCAATCCGGCGATTCTGTCGGCGCTGTCGCCGAACG
CCGATGAGAACCACGACTTCTTCTCCGGCTCCGGCTCGTCATACGTGATTGGCAAAGCGGTCAATACCGAGGATGACGAC
TGGGATTTTTGA

Upstream 100 bases:

>100_bases
GTAAAGGCATCAAAACCATTTATTACATCCGCCTGCGCCAGATGGCGTTGGAAGGCACCGAGGTGCAGGGCTGCGTGTCC
TGTGCACTGTGAGGCAAATT

Downstream 100 bases:

>100_bases
GGTTAATAGCAGGGGCGAATAACTTCGCCTTATAACGATAACCGGTGAACGGGCGCAGCATGCAGCGCCCCTACAATTCA
TTCTTTTGCTAGCTGCACAT

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: R2F protein; Ribonucleotide reductase 2 [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNSIKPAQLVRAVNWNIIEDDKDLEVWNRLTSNFWLPEKVPLSNDIPSWATLTPKEQQLTIRVFTGLTLLDTIQNTVGAP
ALIADAITPHEEAVYSNISFMEAVHARSYSSIFSTLCQTPDVDDAYRWSEENRALQKKASIILAHYRSDDPLMKKVASVF
LESFLFYSGFYLPMYWSSRAKLTNTADLIRLIIRDEAVHGYYIGYKFQKGLEKVDAARRQQVKNFAFDLLQDLYDNEVRY
TEELYDGVGWTEDVKTFLHYNANKALMNLGYEALFPPSMAEVNPAILSALSPNADENHDFFSGSGSSYVIGKAVNTEDDD
WDF

Sequences:

>Translated_323_residues
MNSIKPAQLVRAVNWNIIEDDKDLEVWNRLTSNFWLPEKVPLSNDIPSWATLTPKEQQLTIRVFTGLTLLDTIQNTVGAP
ALIADAITPHEEAVYSNISFMEAVHARSYSSIFSTLCQTPDVDDAYRWSEENRALQKKASIILAHYRSDDPLMKKVASVF
LESFLFYSGFYLPMYWSSRAKLTNTADLIRLIIRDEAVHGYYIGYKFQKGLEKVDAARRQQVKNFAFDLLQDLYDNEVRY
TEELYDGVGWTEDVKTFLHYNANKALMNLGYEALFPPSMAEVNPAILSALSPNADENHDFFSGSGSSYVIGKAVNTEDDD
WDF
>Mature_323_residues
MNSIKPAQLVRAVNWNIIEDDKDLEVWNRLTSNFWLPEKVPLSNDIPSWATLTPKEQQLTIRVFTGLTLLDTIQNTVGAP
ALIADAITPHEEAVYSNISFMEAVHARSYSSIFSTLCQTPDVDDAYRWSEENRALQKKASIILAHYRSDDPLMKKVASVF
LESFLFYSGFYLPMYWSSRAKLTNTADLIRLIIRDEAVHGYYIGYKFQKGLEKVDAARRQQVKNFAFDLLQDLYDNEVRY
TEELYDGVGWTEDVKTFLHYNANKALMNLGYEALFPPSMAEVNPAILSALSPNADENHDFFSGSGSSYVIGKAVNTEDDD
WDF

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2F contains the tyrosyl radical required for catalysis [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1789031, Length=314, Percent_Identity=78.9808917197452, Blast_Score=525, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 36819; Mature: 36819

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSIKPAQLVRAVNWNIIEDDKDLEVWNRLTSNFWLPEKVPLSNDIPSWATLTPKEQQLT
CCCCCHHHHHHHHCCCEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEE
IRVFTGLTLLDTIQNTVGAPALIADAITPHEEAVYSNISFMEAVHARSYSSIFSTLCQTP
EEEHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DVDDAYRWSEENRALQKKASIILAHYRSDDPLMKKVASVFLESFLFYSGFYLPMYWSSRA
CCCHHHHHCHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
KLTNTADLIRLIIRDEAVHGYYIGYKFQKGLEKVDAARRQQVKNFAFDLLQDLYDNEVRY
CCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
TEELYDGVGWTEDVKTFLHYNANKALMNLGYEALFPPSMAEVNPAILSALSPNADENHDF
HHHHHCCCCCHHHHHHHHHCCCCHHHHHCCHHHCCCCCHHHCCHHHHHHCCCCCCCCCCC
FSGSGSSYVIGKAVNTEDDDWDF
CCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MNSIKPAQLVRAVNWNIIEDDKDLEVWNRLTSNFWLPEKVPLSNDIPSWATLTPKEQQLT
CCCCCHHHHHHHHCCCEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEE
IRVFTGLTLLDTIQNTVGAPALIADAITPHEEAVYSNISFMEAVHARSYSSIFSTLCQTP
EEEHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DVDDAYRWSEENRALQKKASIILAHYRSDDPLMKKVASVFLESFLFYSGFYLPMYWSSRA
CCCHHHHHCHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
KLTNTADLIRLIIRDEAVHGYYIGYKFQKGLEKVDAARRQQVKNFAFDLLQDLYDNEVRY
CCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
TEELYDGVGWTEDVKTFLHYNANKALMNLGYEALFPPSMAEVNPAILSALSPNADENHDF
HHHHHCCCCCHHHHHHHHHCCCCHHHHHCCHHHCCCCCHHHCCHHHHHHCCCCCCCCCCC
FSGSGSSYVIGKAVNTEDDDWDF
CCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8195103; 11677609; 2691838; 2548994; 9748343 [H]