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The map label for this gene is nrdE [H]

Identifier: 157371958

GI number: 157371958

Start: 4110857

End: 4113001

Strand: Direct

Name: nrdE [H]

Synonym: Spro_3723

Alternate gene names: 157371958

Gene position: 4110857-4113001 (Clockwise)

Preceding gene: 157371957

Following gene: 157371959

Centisome position: 75.44

GC content: 55.38

Gene sequence:

>2145_bases
TTGGCAACGACAGAACTGACCAGGCCCACCACCAGCGCGCTGGATTATCATTCGCTCAACGCGATGCTCAATCTTTATGA
TGCCGAAGGCCGCATCCAGTTTGATAAGGATCGACTGGCGGCACGACAATACTTCCTGCAGCACGTGAATCAAAACACCG
TGTTCTTCCACAACCTGGAAGAAAAGCTGCGCTATTTGGTGGAAGAAGGCTATTACGAGCAAAGCGTGCTGGCGCAATAC
GACTTTGCCTTTATCAAGCAGTTGTTCCAGCAGGCCTACGCGAAAAAATTCCGCTTCGAGACCTTTCTCGGCGCCTTTAA
GTACTACACCAGCTACACGCTGAAAACCTTTGACGGCAAACGCTATCTGGAACGCTATGAAGACCGGGTGTGCATGGTCG
CCATGACGCTGGCCGCAGGCGATACCGCACTGGCGCAGGATTTGGTAGAAGAAATGATCTCCGGTCGCTTCCAGCCGGCG
ACGCCGACCTTCCTCAACTGCGGCAAACAGCAGCGCGGCGAACTGGTTTCCTGCTTCCTGCTGCGCATTGAAGACAACAT
GGAGTCGATTGGCCGGGCGGTGAATTCTGCGCTGCAACTGTCGAAACGCGGCGGCGGCGTGGCATTTTTGCTGACCAATA
TCCGTGAAGTCGGTGCACCGATCAAACGCATTGAGAACCAATCCTCCGGCGTCATTCCGATCATGAAAATGCTCGAAGAC
GCCTTCTCTTACGCCAACCAACTGGGCGCGCGTCAGGGCGCCGGAGCGGTCTATCTCAATGCTCACCACCCGGATATCCT
GCGTTTTCTCGATACCAAGCGCGAAAACGCCGACGAGAAGATCCGCATTAAAACGCTGTCGCTGGGGGTGGTGATCCCGG
ATATCACCTTCGAGCTGGCGAAAAACAACGAAGAGATGTACCTGTTCTCGCCGTATGACGTCGAGCAGGTGTATGGCGTG
CCCTTCTCGGAAATCAGCGTCAGCGAGAAATATCGCGAAATGGTGGATGACAAGCGCATCCGCAAATCACGCATCAATGC
GCGTGAGTTTTTCCAGGTGCTGGCGGAGATCCAGTTCGAATCTGGTTACCCGTACATGATGTTTGAAGATACCGTCAATC
GTGAGAACCCGATCGCCGGACGCATCAATATGAGTAACCTGTGCTCGGAGATTTTGCAGGTTAACCGCGCCAGCACTTAC
CACGATGACCTGAGCTATGACCAGGTGGGGAAGGATATCTCCTGCAACCTCGGCTCGCTAAACATCGCCAAAACCATGGA
TGCGCCGGACTTCGGCAAAGCCGTCGACACCGCGATCCGTGCGCTGACTGCGGTGGCGGACATGAGCGACATTCGTTCGG
TGCCGTCGATCGCCGAAGGCAATCGCAGCTCGCACGCCATCGGTCTGGGCCAGATGAACCTGCATGGCTATCTGGCACGC
GAACGCATCTTTTATGGCTCGGAGGAAGGGATTGATTTCACCAACATCTATTTCTACGCCGTTGCTTACCACGCCATTCG
CGCCTCTAACCAATTGGCGATTGAACGCGGCAGCGCCTTCGGCGGCTTTGAGGATTCCACCTACGCCTCCGGTGAGTATT
TCAGCAAATACACCGAGCGGGATTGGCTGCCACAGACCGAGCGAGTTCGCACACTGTTCGCCGACGCCAATATCGCCATT
CCAGGCCGTGAAGAGTGGCTGGCACTGCGCCAGTCGGTGATGATGCACGGGCTGTACAACCAGAATCTGCAGGCGGTGCC
ACCAACCGGTTCCATTTCTTACATCAATAATTCGACCTCCAGCATTCATCCGATTGTGTCACGCATCGAGATCCGCAAAG
AGGGCAAGATTGGTCGCGTGTATTATCCGGCGCCCTATATGACCAACGACAACCTGGAGTACTACCAGGATGCCTATGAC
ATCGGGCCGGAAAAGATCATTGATACCTATGCCGCCGCCAGCCAGCACGTCGATCAGGGATTGTCGCTGACGTTGTTCTT
CCGCGATACCGCCACTACGCGCGACATCAACCGCGCGCAGATTTACGCCTGGCGTAAAGGCATCAAAACCATTTATTACA
TCCGCCTGCGCCAGATGGCGTTGGAAGGCACCGAGGTGCAGGGCTGCGTGTCCTGTGCACTGTGA

Upstream 100 bases:

>100_bases
ACATCATCGCCAAAAAATGTCAGGTGCCTTTTCTTTACCGTTTTGAACTGCTCGGCACCACACAAGACGTTGCAAACGTT
CGACAGGGAGTAACCGCATT

Downstream 100 bases:

>100_bases
GGCAAATTATGAATAGCATTAAACCGGCGCAACTGGTGCGCGCAGTCAACTGGAACATCATCGAAGACGACAAGGATCTG
GAGGTGTGGAACCGTCTGAC

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 714; Mature: 713

Protein sequence:

>714_residues
MATTELTRPTTSALDYHSLNAMLNLYDAEGRIQFDKDRLAARQYFLQHVNQNTVFFHNLEEKLRYLVEEGYYEQSVLAQY
DFAFIKQLFQQAYAKKFRFETFLGAFKYYTSYTLKTFDGKRYLERYEDRVCMVAMTLAAGDTALAQDLVEEMISGRFQPA
TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLTNIREVGAPIKRIENQSSGVIPIMKMLED
AFSYANQLGARQGAGAVYLNAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKNNEEMYLFSPYDVEQVYGV
PFSEISVSEKYREMVDDKRIRKSRINAREFFQVLAEIQFESGYPYMMFEDTVNRENPIAGRINMSNLCSEILQVNRASTY
HDDLSYDQVGKDISCNLGSLNIAKTMDAPDFGKAVDTAIRALTAVADMSDIRSVPSIAEGNRSSHAIGLGQMNLHGYLAR
ERIFYGSEEGIDFTNIYFYAVAYHAIRASNQLAIERGSAFGGFEDSTYASGEYFSKYTERDWLPQTERVRTLFADANIAI
PGREEWLALRQSVMMHGLYNQNLQAVPPTGSISYINNSTSSIHPIVSRIEIRKEGKIGRVYYPAPYMTNDNLEYYQDAYD
IGPEKIIDTYAAASQHVDQGLSLTLFFRDTATTRDINRAQIYAWRKGIKTIYYIRLRQMALEGTEVQGCVSCAL

Sequences:

>Translated_714_residues
MATTELTRPTTSALDYHSLNAMLNLYDAEGRIQFDKDRLAARQYFLQHVNQNTVFFHNLEEKLRYLVEEGYYEQSVLAQY
DFAFIKQLFQQAYAKKFRFETFLGAFKYYTSYTLKTFDGKRYLERYEDRVCMVAMTLAAGDTALAQDLVEEMISGRFQPA
TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLTNIREVGAPIKRIENQSSGVIPIMKMLED
AFSYANQLGARQGAGAVYLNAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKNNEEMYLFSPYDVEQVYGV
PFSEISVSEKYREMVDDKRIRKSRINAREFFQVLAEIQFESGYPYMMFEDTVNRENPIAGRINMSNLCSEILQVNRASTY
HDDLSYDQVGKDISCNLGSLNIAKTMDAPDFGKAVDTAIRALTAVADMSDIRSVPSIAEGNRSSHAIGLGQMNLHGYLAR
ERIFYGSEEGIDFTNIYFYAVAYHAIRASNQLAIERGSAFGGFEDSTYASGEYFSKYTERDWLPQTERVRTLFADANIAI
PGREEWLALRQSVMMHGLYNQNLQAVPPTGSISYINNSTSSIHPIVSRIEIRKEGKIGRVYYPAPYMTNDNLEYYQDAYD
IGPEKIIDTYAAASQHVDQGLSLTLFFRDTATTRDINRAQIYAWRKGIKTIYYIRLRQMALEGTEVQGCVSCAL
>Mature_713_residues
ATTELTRPTTSALDYHSLNAMLNLYDAEGRIQFDKDRLAARQYFLQHVNQNTVFFHNLEEKLRYLVEEGYYEQSVLAQYD
FAFIKQLFQQAYAKKFRFETFLGAFKYYTSYTLKTFDGKRYLERYEDRVCMVAMTLAAGDTALAQDLVEEMISGRFQPAT
PTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLTNIREVGAPIKRIENQSSGVIPIMKMLEDA
FSYANQLGARQGAGAVYLNAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKNNEEMYLFSPYDVEQVYGVP
FSEISVSEKYREMVDDKRIRKSRINAREFFQVLAEIQFESGYPYMMFEDTVNRENPIAGRINMSNLCSEILQVNRASTYH
DDLSYDQVGKDISCNLGSLNIAKTMDAPDFGKAVDTAIRALTAVADMSDIRSVPSIAEGNRSSHAIGLGQMNLHGYLARE
RIFYGSEEGIDFTNIYFYAVAYHAIRASNQLAIERGSAFGGFEDSTYASGEYFSKYTERDWLPQTERVRTLFADANIAIP
GREEWLALRQSVMMHGLYNQNLQAVPPTGSISYINNSTSSIHPIVSRIEIRKEGKIGRVYYPAPYMTNDNLEYYQDAYDI
GPEKIIDTYAAASQHVDQGLSLTLFFRDTATTRDINRAQIYAWRKGIKTIYYIRLRQMALEGTEVQGCVSCAL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=656, Percent_Identity=25.3048780487805, Blast_Score=181, Evalue=3e-45,
Organism=Escherichia coli, GI1789030, Length=714, Percent_Identity=73.9495798319328, Blast_Score=1119, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=560, Percent_Identity=26.7857142857143, Blast_Score=179, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17555242, Length=642, Percent_Identity=25.0778816199377, Blast_Score=174, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6320914, Length=654, Percent_Identity=25.5351681957187, Blast_Score=177, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6322123, Length=653, Percent_Identity=25.5742725880551, Blast_Score=177, Evalue=6e-45,
Organism=Drosophila melanogaster, GI17352481, Length=638, Percent_Identity=24.9216300940439, Blast_Score=164, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 81194; Mature: 81063

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTELTRPTTSALDYHSLNAMLNLYDAEGRIQFDKDRLAARQYFLQHVNQNTVFFHNLE
CCCCCCCCCCHHHHHHHHHHHHHEEEECCCCEEECHHHHHHHHHHHHHCCCCEEEEECHH
EKLRYLVEEGYYEQSVLAQYDFAFIKQLFQQAYAKKFRFETFLGAFKYYTSYTLKTFDGK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCH
RYLERYEDRVCMVAMTLAAGDTALAQDLVEEMISGRFQPATPTFLNCGKQQRGELVSCFL
HHHHHHHCCEEEEEEEHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHCCHHHHHH
LRIEDNMESIGRAVNSALQLSKRGGGVAFLLTNIREVGAPIKRIENQSSGVIPIMKMLED
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHH
AFSYANQLGARQGAGAVYLNAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFELA
HHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEE
KNNEEMYLFSPYDVEQVYGVPFSEISVSEKYREMVDDKRIRKSRINAREFFQVLAEIQFE
CCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
SGYPYMMFEDTVNRENPIAGRINMSNLCSEILQVNRASTYHDDLSYDQVGKDISCNLGSL
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCE
NIAKTMDAPDFGKAVDTAIRALTAVADMSDIRSVPSIAEGNRSSHAIGLGQMNLHGYLAR
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEECCCCHHHHHHH
ERIFYGSEEGIDFTNIYFYAVAYHAIRASNQLAIERGSAFGGFEDSTYASGEYFSKYTER
HHEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHCC
DWLPQTERVRTLFADANIAIPGREEWLALRQSVMMHGLYNQNLQAVPPTGSISYINNSTS
CCCCCHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEECCCCC
SIHPIVSRIEIRKEGKIGRVYYPAPYMTNDNLEYYQDAYDIGPEKIIDTYAAASQHVDQG
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
LSLTLFFRDTATTRDINRAQIYAWRKGIKTIYYIRLRQMALEGTEVQGCVSCAL
CEEEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure 
ATTELTRPTTSALDYHSLNAMLNLYDAEGRIQFDKDRLAARQYFLQHVNQNTVFFHNLE
CCCCCCCCCHHHHHHHHHHHHHEEEECCCCEEECHHHHHHHHHHHHHCCCCEEEEECHH
EKLRYLVEEGYYEQSVLAQYDFAFIKQLFQQAYAKKFRFETFLGAFKYYTSYTLKTFDGK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCH
RYLERYEDRVCMVAMTLAAGDTALAQDLVEEMISGRFQPATPTFLNCGKQQRGELVSCFL
HHHHHHHCCEEEEEEEHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHCCHHHHHH
LRIEDNMESIGRAVNSALQLSKRGGGVAFLLTNIREVGAPIKRIENQSSGVIPIMKMLED
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHH
AFSYANQLGARQGAGAVYLNAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFELA
HHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEE
KNNEEMYLFSPYDVEQVYGVPFSEISVSEKYREMVDDKRIRKSRINAREFFQVLAEIQFE
CCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
SGYPYMMFEDTVNRENPIAGRINMSNLCSEILQVNRASTYHDDLSYDQVGKDISCNLGSL
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCE
NIAKTMDAPDFGKAVDTAIRALTAVADMSDIRSVPSIAEGNRSSHAIGLGQMNLHGYLAR
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEECCCCHHHHHHH
ERIFYGSEEGIDFTNIYFYAVAYHAIRASNQLAIERGSAFGGFEDSTYASGEYFSKYTER
HHEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHCC
DWLPQTERVRTLFADANIAIPGREEWLALRQSVMMHGLYNQNLQAVPPTGSISYINNSTS
CCCCCHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEECCCCC
SIHPIVSRIEIRKEGKIGRVYYPAPYMTNDNLEYYQDAYDIGPEKIIDTYAAASQHVDQG
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
LSLTLFFRDTATTRDINRAQIYAWRKGIKTIYYIRLRQMALEGTEVQGCVSCAL
CEEEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]