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The map label for this gene is ppnK

Identifier: 157371920

GI number: 157371920

Start: 4077322

End: 4078200

Strand: Direct

Name: ppnK

Synonym: Spro_3685

Alternate gene names: 157371920

Gene position: 4077322-4078200 (Clockwise)

Preceding gene: 157371918

Following gene: 157371921

Centisome position: 74.83

GC content: 54.15

Gene sequence:

>879_bases
ATGAATAAAAAATTCGCCTGTATTGGCATTGTTGGCCACCCGCGTCACCCTTCGGCGCTGGCAACGCATGAGATGTTATT
TCACTGGCTGGTCGCCCGAGGCTATTCAGTGATGGTTGAACGGCAGATAGCCCAGGATTTAGGGCTGAAAGACGCTGTCA
CCGGCAGCCTGGCAGAGATTGGCCAAAAGGCCGATTTGGCCGTGGTGGTCGGCGGCGACGGTAATATGCTGGGCGCAGCG
CGCGTGCTGGCACGTTACGATATTAAAGTGATCGGTGTTAACCGCGGAAATCTCGGTTTCCTGACCGATCTTGATCCCGA
TAACGCGTTGCAGCAATTGGCCGACGTGCTGGAAGGCGAATATATCGATGAGCAGCGCTTCCTGCTGGAAACCATCGTCC
ACAAGGAAAACCAGCAGTGCCGCATCAGCACCGCTATCAACGAAGTGGTGTTGCACCCCGGTAAAGTGGCGCACATGATT
GAGTTCGAAGTGTATATCGACGATCGCTTCGCCTTCTCACAGCGCTCTGACGGCCTGATCATCGCCACCCCGACTGGTTC
GACCGCCTATTCGCTCTCTGCCGGCGGGCCGATCCTGACGCCGTCGCTGGAAGCCATCGCGCTGGTGCCGATGTTCCCGC
ACACCCTTTCAGCCCGCCCGTTGGTGATCAACGGCAACAGCACTATCCGGCTGAAGTTTTCGCAGATTGGTAGCGATCTG
GAGATCAGCTGCGACAGCCAAATCGCGTTGCCTATCCAGGAAGGTGAAGAGGTATTGATACGCCGTAGTGATTTCCATTT
GAATCTTATTCATCCAAAGGACTACAGCTATTTCAATACGTTAAGCACCAAGCTGGGTTGGTCAAAAAAATTATTCTAA

Upstream 100 bases:

>100_bases
ATCTCGCAACTTGGCTTATTATGGGGATCAAAACCGGGGTTTCAAGTCAACCGGTCACAATGTGGGGCATAAACTGGTCC
CGGTGAGGAAAATCACAACA

Downstream 100 bases:

>100_bases
AAACTGCGCCAACCTCTTTACTGTATATAAAACCAGTCTATACTGTATGAAACCACAGTTATGTATTTATACACAGGAAG
GTTCCCATGCTGGCTCAATT

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MNKKFACIGIVGHPRHPSALATHEMLFHWLVARGYSVMVERQIAQDLGLKDAVTGSLAEIGQKADLAVVVGGDGNMLGAA
RVLARYDIKVIGVNRGNLGFLTDLDPDNALQQLADVLEGEYIDEQRFLLETIVHKENQQCRISTAINEVVLHPGKVAHMI
EFEVYIDDRFAFSQRSDGLIIATPTGSTAYSLSAGGPILTPSLEAIALVPMFPHTLSARPLVINGNSTIRLKFSQIGSDL
EISCDSQIALPIQEGEEVLIRRSDFHLNLIHPKDYSYFNTLSTKLGWSKKLF

Sequences:

>Translated_292_residues
MNKKFACIGIVGHPRHPSALATHEMLFHWLVARGYSVMVERQIAQDLGLKDAVTGSLAEIGQKADLAVVVGGDGNMLGAA
RVLARYDIKVIGVNRGNLGFLTDLDPDNALQQLADVLEGEYIDEQRFLLETIVHKENQQCRISTAINEVVLHPGKVAHMI
EFEVYIDDRFAFSQRSDGLIIATPTGSTAYSLSAGGPILTPSLEAIALVPMFPHTLSARPLVINGNSTIRLKFSQIGSDL
EISCDSQIALPIQEGEEVLIRRSDFHLNLIHPKDYSYFNTLSTKLGWSKKLF
>Mature_292_residues
MNKKFACIGIVGHPRHPSALATHEMLFHWLVARGYSVMVERQIAQDLGLKDAVTGSLAEIGQKADLAVVVGGDGNMLGAA
RVLARYDIKVIGVNRGNLGFLTDLDPDNALQQLADVLEGEYIDEQRFLLETIVHKENQQCRISTAINEVVLHPGKVAHMI
EFEVYIDDRFAFSQRSDGLIIATPTGSTAYSLSAGGPILTPSLEAIALVPMFPHTLSARPLVINGNSTIRLKFSQIGSDL
EISCDSQIALPIQEGEEVLIRRSDFHLNLIHPKDYSYFNTLSTKLGWSKKLF

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=328, Percent_Identity=25.9146341463415, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1788968, Length=292, Percent_Identity=82.8767123287671, Blast_Score=503, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6320794, Length=243, Percent_Identity=30.4526748971193, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6325068, Length=189, Percent_Identity=37.037037037037, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6322509, Length=240, Percent_Identity=30, Blast_Score=114, Evalue=1e-26,
Organism=Drosophila melanogaster, GI28573832, Length=307, Percent_Identity=27.0358306188925, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28573828, Length=307, Percent_Identity=27.0358306188925, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28573830, Length=307, Percent_Identity=27.0358306188925, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28573826, Length=307, Percent_Identity=27.0358306188925, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI161077047, Length=307, Percent_Identity=27.0358306188925, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI20129957, Length=276, Percent_Identity=25, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281363323, Length=276, Percent_Identity=25, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653422, Length=276, Percent_Identity=25, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281363321, Length=276, Percent_Identity=25, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653424, Length=276, Percent_Identity=25, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_SERP5 (A8GI41)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479909.1
- ProteinModelPortal:   A8GI41
- SMR:   A8GI41
- STRING:   A8GI41
- GeneID:   5606970
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3685
- eggNOG:   COG0061
- HOGENOM:   HBG713904
- OMA:   DGTMLNA
- ProtClustDB:   PRK03378
- BioCyc:   SPRO399741:SPRO_3685-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 32092; Mature: 32092

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKFACIGIVGHPRHPSALATHEMLFHWLVARGYSVMVERQIAQDLGLKDAVTGSLAEI
CCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCHHEEHHHHHHHHCCCCHHCCCCHHHC
GQKADLAVVVGGDGNMLGAARVLARYDIKVIGVNRGNLGFLTDLDPDNALQQLADVLEGE
CCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHCCC
YIDEQRFLLETIVHKENQQCRISTAINEVVLHPGKVAHMIEFEVYIDDRFAFSQRSDGLI
CCCHHHHHHHHHHCCCCCCEEEEHHHHHHHCCCCCEEEEEEEEEEEECEEEECCCCCCEE
IATPTGSTAYSLSAGGPILTPSLEAIALVPMFPHTLSARPLVINGNSTIRLKFSQIGSDL
EEECCCCCEEEECCCCCEECCCCCEEEEEECCCCCCCCCEEEECCCCEEEEEHHHCCCCE
EISCDSQIALPIQEGEEVLIRRSDFHLNLIHPKDYSYFNTLSTKLGWSKKLF
EEECCCEEEEECCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNKKFACIGIVGHPRHPSALATHEMLFHWLVARGYSVMVERQIAQDLGLKDAVTGSLAEI
CCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCHHEEHHHHHHHHCCCCHHCCCCHHHC
GQKADLAVVVGGDGNMLGAARVLARYDIKVIGVNRGNLGFLTDLDPDNALQQLADVLEGE
CCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHCCC
YIDEQRFLLETIVHKENQQCRISTAINEVVLHPGKVAHMIEFEVYIDDRFAFSQRSDGLI
CCCHHHHHHHHHHCCCCCCEEEEHHHHHHHCCCCCEEEEEEEEEEEECEEEECCCCCCEE
IATPTGSTAYSLSAGGPILTPSLEAIALVPMFPHTLSARPLVINGNSTIRLKFSQIGSDL
EEECCCCCEEEECCCCCEECCCCCEEEEEECCCCCCCCCEEEECCCCEEEEEHHHCCCCE
EISCDSQIALPIQEGEEVLIRRSDFHLNLIHPKDYSYFNTLSTKLGWSKKLF
EEECCCEEEEECCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA