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The map label for this gene is nadB [H]

Identifier: 157371912

GI number: 157371912

Start: 4069320

End: 4070957

Strand: Direct

Name: nadB [H]

Synonym: Spro_3677

Alternate gene names: 157371912

Gene position: 4069320-4070957 (Clockwise)

Preceding gene: 157371907

Following gene: 157371914

Centisome position: 74.68

GC content: 58.06

Gene sequence:

>1638_bases
ATGAATTTCACTCTTCTTCTGCACACATCATACGCTATGCAACCATCATCTGAACATGTTAGCGATGTACTGATCGTCGG
TAGTGGTGCTGCCGGCCTGTCACTGGCGCTGCGCCTGGCGCAGCATTGCAAGGTTACCGTACTCAGCAAGGGGCCGCTCA
ACGAAGGCGCCACATTTTACGCCCAAGGCGGGATCGCCGCGGTTTTCGATGAAACGGACAGCATTGCCTCACACGTAGAT
GACACTTTGATTGCCGGAGCCGGCCTGTGTGACAAAGACGCCGTTGAGTTTATCGCCGGCAATGCGCGCCACTGCGTGCA
GTGGTTGATCGATCAGGGGGTATTGTTCGACACCGAGGTCAACGCTCAGGGCGAAGCACATTACCACCTGACGCGGGAAG
GGGGGCACAGCCACCGCCGTATCCTGCATGCCGCTGATGCTACAGGTAAAGAAGTAGAGACTACCCTGGTGGGGAAAGCA
AGCAACCACCCGAATATTTGCGTGATGGAACGCCGTAATGCGGTCGATCTGATCACCTCGAACAAAATCGGCCTGCCGGG
CACTCGTCGGGTGGTCGGCGCCTATGTGTGGAACCGCGAGCTGGAGCAGGTAGAAACCTACCGCGCCAAAACAGTGGTGC
TGGCTACCGGCGGTGCAGCCAAGGTTTATCAATACACCACCAACCCGGATATCTCATCCGGCGACGGGATCGCCATGGCC
TGGCGCGCAGGTTGCCGGGTCGCCAACCTTGAATTCAATCAGTTCCACCCGACCTGTCTGTTCCATCCGCAGGCGCGTAA
CTTCCTGCTGACCGAAGCGTTGCGCGGTGAAGGTGCCTATCTGAAGCGCCCGGATGGCAGCCGCTTTATGCCCGATTTTG
ACCCTCGCGGCGAACTGGCACCACGGGACGTCGTTGCGCGAGCCATCGACCACGAAATGAAGCGTCTGGGTGCGGACTGC
ATGTATCTGGACATCAGCCATAAACCAGCTGAATTCATTACCCAGCATTTCCCGATGATCCATGAAAAACTGCTGACGTT
GGGCTTTGACCTGACCAGCCAGCCGATTCCTATCGTCCCTGCCGCGCACTATACCTGCGGCGGCGTGATGGTCGATCAGC
ATGGCCGTACCGACCTTGACGGGCTGTATGCCATTGGCGAGGTCAGCTATACCGGCCTGCACGGCGCTAACCGCATGGCG
TCCAACTCACTGCTGGAATGTCTGGTCTACGGTTGGTCAGCGGCGGAAGACATCATGATGCGTCTGCCGTTTATCAAGCT
GGCGAAACAGCTGCCGCAGTGGGATGAAAGCCGGGTTGACGACGCCGACGAACGCGTGGTGATCCAACATAACTGGCATG
AGTTGCGATTGTTTATGTGGGACTACGTGGGGATCGTGCGCACCACCAAACGGCTGGAGCGCGCATTGCGCCGCATCAAT
ACCTTGCAGGCAGAAATTGACGAGTACTATGCCAACTTCCGCATCTCCAACAACCTGCTGGAACTGCGCAATCTGGTGCA
GGTGGCGGAGCTGATCGTCCGCAGCGCCATGGAGCGCAAGGAAAGCCGCGGGTTGCACTATACGCTGGACTATCCGGATC
TGCTGCCGCAAGCGCTGCCGACCATTCTGCAGCCCTAA

Upstream 100 bases:

>100_bases
ACTCGCTTGGAGTTCATACAGAACACGAAGTTCCGTATTGCCATTGTTTTTTATGTTGATGTCACCCATCCGTGGCTTTT
GCCCGGACAATTAGGCTAAC

Downstream 100 bases:

>100_bases
TGATGCCGCCTGGGCGCCTGACCATGGCGCCCTTTTCATTTCAGTAAAACAAATAGAAATCAGTAATCAGACGGCGAAAA
TCATCGGTGTAACTGCCATC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MNFTLLLHTSYAMQPSSEHVSDVLIVGSGAAGLSLALRLAQHCKVTVLSKGPLNEGATFYAQGGIAAVFDETDSIASHVD
DTLIAGAGLCDKDAVEFIAGNARHCVQWLIDQGVLFDTEVNAQGEAHYHLTREGGHSHRRILHAADATGKEVETTLVGKA
SNHPNICVMERRNAVDLITSNKIGLPGTRRVVGAYVWNRELEQVETYRAKTVVLATGGAAKVYQYTTNPDISSGDGIAMA
WRAGCRVANLEFNQFHPTCLFHPQARNFLLTEALRGEGAYLKRPDGSRFMPDFDPRGELAPRDVVARAIDHEMKRLGADC
MYLDISHKPAEFITQHFPMIHEKLLTLGFDLTSQPIPIVPAAHYTCGGVMVDQHGRTDLDGLYAIGEVSYTGLHGANRMA
SNSLLECLVYGWSAAEDIMMRLPFIKLAKQLPQWDESRVDDADERVVIQHNWHELRLFMWDYVGIVRTTKRLERALRRIN
TLQAEIDEYYANFRISNNLLELRNLVQVAELIVRSAMERKESRGLHYTLDYPDLLPQALPTILQP

Sequences:

>Translated_545_residues
MNFTLLLHTSYAMQPSSEHVSDVLIVGSGAAGLSLALRLAQHCKVTVLSKGPLNEGATFYAQGGIAAVFDETDSIASHVD
DTLIAGAGLCDKDAVEFIAGNARHCVQWLIDQGVLFDTEVNAQGEAHYHLTREGGHSHRRILHAADATGKEVETTLVGKA
SNHPNICVMERRNAVDLITSNKIGLPGTRRVVGAYVWNRELEQVETYRAKTVVLATGGAAKVYQYTTNPDISSGDGIAMA
WRAGCRVANLEFNQFHPTCLFHPQARNFLLTEALRGEGAYLKRPDGSRFMPDFDPRGELAPRDVVARAIDHEMKRLGADC
MYLDISHKPAEFITQHFPMIHEKLLTLGFDLTSQPIPIVPAAHYTCGGVMVDQHGRTDLDGLYAIGEVSYTGLHGANRMA
SNSLLECLVYGWSAAEDIMMRLPFIKLAKQLPQWDESRVDDADERVVIQHNWHELRLFMWDYVGIVRTTKRLERALRRIN
TLQAEIDEYYANFRISNNLLELRNLVQVAELIVRSAMERKESRGLHYTLDYPDLLPQALPTILQP
>Mature_545_residues
MNFTLLLHTSYAMQPSSEHVSDVLIVGSGAAGLSLALRLAQHCKVTVLSKGPLNEGATFYAQGGIAAVFDETDSIASHVD
DTLIAGAGLCDKDAVEFIAGNARHCVQWLIDQGVLFDTEVNAQGEAHYHLTREGGHSHRRILHAADATGKEVETTLVGKA
SNHPNICVMERRNAVDLITSNKIGLPGTRRVVGAYVWNRELEQVETYRAKTVVLATGGAAKVYQYTTNPDISSGDGIAMA
WRAGCRVANLEFNQFHPTCLFHPQARNFLLTEALRGEGAYLKRPDGSRFMPDFDPRGELAPRDVVARAIDHEMKRLGADC
MYLDISHKPAEFITQHFPMIHEKLLTLGFDLTSQPIPIVPAAHYTCGGVMVDQHGRTDLDGLYAIGEVSYTGLHGANRMA
SNSLLECLVYGWSAAEDIMMRLPFIKLAKQLPQWDESRVDDADERVVIQHNWHELRLFMWDYVGIVRTTKRLERALRRIN
TLQAEIDEYYANFRISNNLLELRNLVQVAELIVRSAMERKESRGLHYTLDYPDLLPQALPTILQP

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=511, Percent_Identity=31.8982387475538, Blast_Score=189, Evalue=5e-48,
Organism=Escherichia coli, GI1788928, Length=533, Percent_Identity=82.7392120075047, Blast_Score=919, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=524, Percent_Identity=32.6335877862595, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1786942, Length=560, Percent_Identity=30.8928571428571, Blast_Score=182, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI17550100, Length=535, Percent_Identity=32.1495327102804, Blast_Score=204, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI17505833, Length=534, Percent_Identity=30.1498127340824, Blast_Score=196, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI71986328, Length=289, Percent_Identity=29.757785467128, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322701, Length=527, Percent_Identity=32.4478178368121, Blast_Score=215, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6322416, Length=558, Percent_Identity=32.258064516129, Blast_Score=204, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6320788, Length=482, Percent_Identity=26.5560165975104, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17137288, Length=559, Percent_Identity=31.8425760286225, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24655642, Length=559, Percent_Identity=31.8425760286225, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24655647, Length=559, Percent_Identity=31.8425760286225, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24663005, Length=554, Percent_Identity=29.6028880866426, Blast_Score=182, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 60708; Mature: 60708

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFTLLLHTSYAMQPSSEHVSDVLIVGSGAAGLSLALRLAQHCKVTVLSKGPLNEGATFY
CCEEEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCEEEEEECCCCCCCCEEE
AQGGIAAVFDETDSIASHVDDTLIAGAGLCDKDAVEFIAGNARHCVQWLIDQGVLFDTEV
ECCCEEEEECCHHHHHHHHCHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHCCEEEEEEC
NAQGEAHYHLTREGGHSHRRILHAADATGKEVETTLVGKASNHPNICVMERRNAVDLITS
CCCCCEEEEEECCCCCCHHHEEEECCCCCCEEEEEEEECCCCCCCEEEEECCCCEEEEEC
NKIGLPGTRRVVGAYVWNRELEQVETYRAKTVVLATGGAAKVYQYTTNPDISSGDGIAMA
CCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCEEEEEECCCCCCCCCCEEEE
WRAGCRVANLEFNQFHPTCLFHPQARNFLLTEALRGEGAYLKRPDGSRFMPDFDPRGELA
ECCCCEEEEEECCCCCCEEEECCCCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCC
PRDVVARAIDHEMKRLGADCMYLDISHKPAEFITQHFPMIHEKLLTLGFDLTSQPIPIVP
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
AAHYTCGGVMVDQHGRTDLDGLYAIGEVSYTGLHGANRMASNSLLECLVYGWSAAEDIMM
CCCCCCCCEEEECCCCCCCCCEEEECCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
RLPFIKLAKQLPQWDESRVDDADERVVIQHNWHELRLFMWDYVGIVRTTKRLERALRRIN
HCCHHHHHHHCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLQAEIDEYYANFRISNNLLELRNLVQVAELIVRSAMERKESRGLHYTLDYPDLLPQALP
HHHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHH
TILQP
HHCCC
>Mature Secondary Structure
MNFTLLLHTSYAMQPSSEHVSDVLIVGSGAAGLSLALRLAQHCKVTVLSKGPLNEGATFY
CCEEEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCEEEEEECCCCCCCCEEE
AQGGIAAVFDETDSIASHVDDTLIAGAGLCDKDAVEFIAGNARHCVQWLIDQGVLFDTEV
ECCCEEEEECCHHHHHHHHCHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHCCEEEEEEC
NAQGEAHYHLTREGGHSHRRILHAADATGKEVETTLVGKASNHPNICVMERRNAVDLITS
CCCCCEEEEEECCCCCCHHHEEEECCCCCCEEEEEEEECCCCCCCEEEEECCCCEEEEEC
NKIGLPGTRRVVGAYVWNRELEQVETYRAKTVVLATGGAAKVYQYTTNPDISSGDGIAMA
CCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCEEEEEECCCCCCCCCCEEEE
WRAGCRVANLEFNQFHPTCLFHPQARNFLLTEALRGEGAYLKRPDGSRFMPDFDPRGELA
ECCCCEEEEEECCCCCCEEEECCCCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCC
PRDVVARAIDHEMKRLGADCMYLDISHKPAEFITQHFPMIHEKLLTLGFDLTSQPIPIVP
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
AAHYTCGGVMVDQHGRTDLDGLYAIGEVSYTGLHGANRMASNSLLECLVYGWSAAEDIMM
CCCCCCCCEEEECCCCCCCCCEEEECCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
RLPFIKLAKQLPQWDESRVDDADERVVIQHNWHELRLFMWDYVGIVRTTKRLERALRRIN
HCCHHHHHHHCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLQAEIDEYYANFRISNNLLELRNLVQVAELIVRSAMERKESRGLHYTLDYPDLLPQALP
HHHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHH
TILQP
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]