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The map label for this gene is ybbF [H]

Identifier: 157371895

GI number: 157371895

Start: 4053158

End: 4054525

Strand: Reverse

Name: ybbF [H]

Synonym: Spro_3660

Alternate gene names: 157371895

Gene position: 4054525-4053158 (Counterclockwise)

Preceding gene: 157371896

Following gene: 157371894

Centisome position: 74.41

GC content: 58.63

Gene sequence:

>1368_bases
ATGGACAAGACAACGGCACTGGCGACACAGATTCTGGCGGGCATTGGCGGCGAGGGGAATATCCTTCGGCTGGAGAACTG
CATGACACGAGTACGTGTCGAGGTTAGCGATGAGCAACGCCTTGACCTGGCGGCGCTCAAGCAGTTACCGGGTGTTAAGG
GCTATATCAAACAGGGCGAACAGCATCAGTTTATTGTTGGGCCGGGTGCCGCTGCCAAGGTGGTCGATGCCATGCGTTCA
CTGCTCACCGGCGCACCGGTTGCCGTGGCCAGCGGAGATGACGTCGCCCGTAACAAGGCACAAGCCAAACAAAAGTATGC
TGCGCCAATGAGCGGCGCGCTGAAAAAGTTGGCCGATGTCTTTATTCCGTTGATCCCGGCGTTCATCGCCTCCGGTCTGA
TTACCGGCATCATCAACCTGCTGAAACGGCCGGATATTGCCGGCGAACTGGCGCTAAATTACCCCAACGTGCTGGGACTG
CTGGCGATTTTTGGCAGTGCCGTGTTCGCCATCATGAATATTCTGGTCGGGGTTAACGCCGCCAGGGTGTTTGGCGGTTC
ACAGGCGATGGGCGGCGTCATGGCCGGTATTCTCTCCAGCCCGGCGTTGGCGCAGATCACTTTGTTCGGTGAGGCGTTGC
AGCCGGGCCGTGGCGGGGTGATTGCAGTGCTGCTGGTGGTGGCGTTGATGTGCTGGGTTGAAAGGCGGCTGCGTAATTGG
CTGCCGGAGTCGATCGAGCTGATCCTCAATCCGCTGATCACCACGCTGGTGACCGCATCGTTGGCGATTGTGATCCTGCA
GCCGCTGGGCGGCTATATTTCGGATGCCATCGCCCACGGCGCTAACCTGGCTATCGACAAAGGGGGCCTGCTGGTGGGAG
CAGTGCTGTCCGGGCTGTTTCTGCCGTTGGTGTTGTCCGGTTTGCATCAGGGGCTGGTGCCGATCCACGTCGAGCTGGTG
CAGGCACATGGTTCCAACCCATTGCTGCCGATTTTGGCGATGGCGGGTGTTGGTCAGGTCGGGGCTGCGCTGGCCGTATT
AATGAAAACCCGTAATGCACGGCTGAAAAAGGTGATCAAGGGAGCGTTGCCGGTCGGTATTCTCGGTATTGGCGAGCCCT
TGATTTTCGGGGTGACCTTGCCGCTGGGGAGGCCCTTTGTCGCCGCGTGTCTCGGCGGTGCCGTGGGCGGGGCGCTGATC
AGCTACTGGAAGGTGGCGACGGTGATCACCTTCGGTATCTCGGGTTTACCTTTGGCGTTAACCATTGTCTCGGGTAAGGT
GATGTTGTATCTGACGGGTCTGGTGATTACCATTGCCGCTGGATTTATTTTCACCTGGATAATGGGATTCAACGATCCTG
AGGAGTAG

Upstream 100 bases:

>100_bases
CTGGCCGGTATCGACGCACAAGAGGCGCAGCAGCGCTTACAACAGCATGACGGCTATTTGCGCGCGGCATTGACGCGCTA
AACCACACCTGGGGCGGGCG

Downstream 100 bases:

>100_bases
CGCTTGAGCGAGAAACAGAGCCAACAGGCCGCGGAAGGCCGCCGTGTGGTGTTCTTTGATCTGGACGGCACGCTGCATCA
GGAAGACATGTTTGGTAGTT

Product: phosphotransferase system EIIC

Products: NA

Alternate protein names: Phosphotransferase enzyme IIB component; PTS system EIIB component; Permease IIC component; PTS system EIIC component [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MDKTTALATQILAGIGGEGNILRLENCMTRVRVEVSDEQRLDLAALKQLPGVKGYIKQGEQHQFIVGPGAAAKVVDAMRS
LLTGAPVAVASGDDVARNKAQAKQKYAAPMSGALKKLADVFIPLIPAFIASGLITGIINLLKRPDIAGELALNYPNVLGL
LAIFGSAVFAIMNILVGVNAARVFGGSQAMGGVMAGILSSPALAQITLFGEALQPGRGGVIAVLLVVALMCWVERRLRNW
LPESIELILNPLITTLVTASLAIVILQPLGGYISDAIAHGANLAIDKGGLLVGAVLSGLFLPLVLSGLHQGLVPIHVELV
QAHGSNPLLPILAMAGVGQVGAALAVLMKTRNARLKKVIKGALPVGILGIGEPLIFGVTLPLGRPFVAACLGGAVGGALI
SYWKVATVITFGISGLPLALTIVSGKVMLYLTGLVITIAAGFIFTWIMGFNDPEE

Sequences:

>Translated_455_residues
MDKTTALATQILAGIGGEGNILRLENCMTRVRVEVSDEQRLDLAALKQLPGVKGYIKQGEQHQFIVGPGAAAKVVDAMRS
LLTGAPVAVASGDDVARNKAQAKQKYAAPMSGALKKLADVFIPLIPAFIASGLITGIINLLKRPDIAGELALNYPNVLGL
LAIFGSAVFAIMNILVGVNAARVFGGSQAMGGVMAGILSSPALAQITLFGEALQPGRGGVIAVLLVVALMCWVERRLRNW
LPESIELILNPLITTLVTASLAIVILQPLGGYISDAIAHGANLAIDKGGLLVGAVLSGLFLPLVLSGLHQGLVPIHVELV
QAHGSNPLLPILAMAGVGQVGAALAVLMKTRNARLKKVIKGALPVGILGIGEPLIFGVTLPLGRPFVAACLGGAVGGALI
SYWKVATVITFGISGLPLALTIVSGKVMLYLTGLVITIAAGFIFTWIMGFNDPEE
>Mature_455_residues
MDKTTALATQILAGIGGEGNILRLENCMTRVRVEVSDEQRLDLAALKQLPGVKGYIKQGEQHQFIVGPGAAAKVVDAMRS
LLTGAPVAVASGDDVARNKAQAKQKYAAPMSGALKKLADVFIPLIPAFIASGLITGIINLLKRPDIAGELALNYPNVLGL
LAIFGSAVFAIMNILVGVNAARVFGGSQAMGGVMAGILSSPALAQITLFGEALQPGRGGVIAVLLVVALMCWVERRLRNW
LPESIELILNPLITTLVTASLAIVILQPLGGYISDAIAHGANLAIDKGGLLVGAVLSGLFLPLVLSGLHQGLVPIHVELV
QAHGSNPLLPILAMAGVGQVGAALAVLMKTRNARLKKVIKGALPVGILGIGEPLIFGVTLPLGRPFVAACLGGAVGGALI
SYWKVATVITFGISGLPLALTIVSGKVMLYLTGLVITIAAGFIFTWIMGFNDPEE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788769, Length=476, Percent_Identity=39.0756302521008, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI1790159, Length=467, Percent_Identity=29.7644539614561, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI2367362, Length=404, Percent_Identity=26.7326732673267, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI48994906, Length=473, Percent_Identity=26.4270613107822, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 47038; Mature: 47038

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKTTALATQILAGIGGEGNILRLENCMTRVRVEVSDEQRLDLAALKQLPGVKGYIKQGE
CCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHEEEECCCHHHHHHHHHHCCCCCHHHCCCC
QHQFIVGPGAAAKVVDAMRSLLTGAPVAVASGDDVARNKAQAKQKYAAPMSGALKKLADV
CCEEEECCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
FIPLIPAFIASGLITGIINLLKRPDIAGELALNYPNVLGLLAIFGSAVFAIMNILVGVNA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
ARVFGGSQAMGGVMAGILSSPALAQITLFGEALQPGRGGVIAVLLVVALMCWVERRLRNW
HHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LPESIELILNPLITTLVTASLAIVILQPLGGYISDAIAHGANLAIDKGGLLVGAVLSGLF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCEEECCCCHHHHHHHHHHH
LPLVLSGLHQGLVPIHVELVQAHGSNPLLPILAMAGVGQVGAALAVLMKTRNARLKKVIK
HHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GALPVGILGIGEPLIFGVTLPLGRPFVAACLGGAVGGALISYWKVATVITFGISGLPLAL
HCCCCCEEECCCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
TIVSGKVMLYLTGLVITIAAGFIFTWIMGFNDPEE
HHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDKTTALATQILAGIGGEGNILRLENCMTRVRVEVSDEQRLDLAALKQLPGVKGYIKQGE
CCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHEEEECCCHHHHHHHHHHCCCCCHHHCCCC
QHQFIVGPGAAAKVVDAMRSLLTGAPVAVASGDDVARNKAQAKQKYAAPMSGALKKLADV
CCEEEECCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
FIPLIPAFIASGLITGIINLLKRPDIAGELALNYPNVLGLLAIFGSAVFAIMNILVGVNA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
ARVFGGSQAMGGVMAGILSSPALAQITLFGEALQPGRGGVIAVLLVVALMCWVERRLRNW
HHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LPESIELILNPLITTLVTASLAIVILQPLGGYISDAIAHGANLAIDKGGLLVGAVLSGLF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCEEECCCCHHHHHHHHHHH
LPLVLSGLHQGLVPIHVELVQAHGSNPLLPILAMAGVGQVGAALAVLMKTRNARLKKVIK
HHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GALPVGILGIGEPLIFGVTLPLGRPFVAACLGGAVGGALISYWKVATVITFGISGLPLAL
HCCCCCEEECCCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
TIVSGKVMLYLTGLVITIAAGFIFTWIMGFNDPEE
HHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]